(DPPH) -1-2 2 3 2 1 1 * -1-2 -3 : * (021) 44196524 : (021) 44196522 : 14115-336 : mbb@modares.ac.ir : 85/9/10 : 84/8/6 : : (Thymus vulgaris L.) GC/MS. :. (Syzygium aromaticum L.) (Satureja hortensis L.) :. (DPPH ) -1-2 2. 3 2 1 0/5 180 120. ( ) :... DPPH :. : 1 54
....[11 20]. 1.[20] 1.[11 20]. 14-21 70-80.[11 20].[18 19].. -1- -2 2 2.[21] (DPPH ) DPPH ) 515 ( 12500 M -1 cm -1 515 DPPH DPPH DPPH.[21] 515-517 DPPH.[21 22].[23] DPPH.[21] DPPH 515-517 DPPH.[21] 5 :.[24 25] 30 5-30 1 Myrtus cummunis L. 2 2,2-diphenyl-1-picrylhydrazyl radical..[1 2 3]. -..[4 5]. TBHQ BHA BHT.[6 7 8].[9 10].[11] -.[12 13 14 15 16 17 18]. 1 55 1386
Carvacrol Thymol Eugenol -1 83-84. GC/MS. DPPH. ( ) GC/MS HP 6890N 0/25 30 split split. HP-5MS 0/25 :. 20:1 :ratio 40 240 1/08 280 3 10 250. 6. 1cm/s HP 5973N. DPPH + AH DPPH LH + A DPPH + R DPPH R DPPH ( ) DPPH ( ).[21]. 30 15 ( ) IC50 50.[21] DPPH.. ١ ( ) 3 2 ( ) 1 Thymus vulgaris L. 3 Syzygium aromaticum L. 2 Satureja hortensis L. 56
... 3. 2 ( ) 5 ( 180 120). ( 3 2 1 0/5 0). 3 2 1 ( 19/59) ( 33/14) ( 6/52) ( 16/00) 21/59) ( 22/16) 10/30) ( 12/21) ( ( 72/37) ( ( 13/14) ( 15/94).. DPPH 2. 180 120. 5 4 3 EI 69/9. 230 200 180 120 ) Memert 3 2 1 0/5 DPPH (. (mg/ml) 1/4 DPPH 0/2 0/6 30 515. 0/6 1/4. DPPH ١ DPPH. =A Sample (A %RSA = = %RSA. = A Control.[3] Control A A Control Sample ) 100 IC50. IC50 50. DPPH 1 Radical scavenging activity (RSA) 57 1386
-1 ( ) 0/12 10/59 α-phellandrene 1/84 0/34 α-pinene 0/34 0/53 12/93 13/14 β -Pinene β-octanone 4 0/72 14/27 α-terpinene 6/52 15/23 ρ-cymene 0/44 15/35 Limonene 0/67 15/50 1,8-Cineol 2/43 16/77 γ-terpinene 16/00 19/07 Linalool 0/51 19/14 Ho-trienol 0/55 22/68 3-Cyclohexene 0/42 23/32 α-terpineol 0/81 23/57 Estragol 0/88 25/12 Methyl thymylether 19/59 25/67 Carvacrol 0/35 27/72 Trans-anethol 33/14 28/36 Thymol 0/50 30/90 Thymyl acetate 0/11 31/13 Neryl acetate 0/81 31/94 Granyl acetate 2/03 33/94 α-caryophyllene 0/17 35/41 β-humelen 1/52 40/83 Caryophyllene oxide 0/25 42/84 Vulgarol 0/15 43/58 Aromandrene 58
... -2 1/24 1/77 0/15 0/92 1/98 0/12 2/93 10/30 1/55 12/21 0/17 21/59 22/16 0/75 0/34 ( ) 10/61 10/99 11/66 12/96 13/16 14/44 14/79 15/36 15/94 17/02 22/8 28/87 29/18 31/88 34/86 α-thujene α-pinene Camphene β-pinene Myrecene α-phellandrene α-terpinene ρ-cymene Limonene γ-terpinene Terpinene-4-ol Carvacrol Thymol Carvacryl acetate Aromanderene -3 0/10 0/34 72/37 15/94 2/62 0/44 13/14 0/18 1/06 ( ) 26/81 30/58 31/51 43/29 35/29 37/18 38/26 40/63 40/87 Chavicol Cubenere Eugenol β-caryophyllene α-humelene E,E-Frances Eugeyl acetate +Spathulenol Caryophyllene oxide 59 1386
IC50 (mg) 12 10 8 6 4 2 0 c T0 T120 T180 :T0.( 3) (IC50) -2 180 3 :T180 120 3 :T120. 11 10 A120 A180 IC50 (mg) 9 8 7 0 1 2 3 ( ) A180 120 A120-3. 180 IC50 (mg) 7 S120 6.5 S180 6 5.5 5 4.5 4 0 1 2 3 ( ) S180 120 S120-4 180 60
... 0.5 0.45 C120 C180 IC50 (mg) 0.4 0.35 0.3 0 1 2 3 ( ) C180 120 C120-5. 180. 1 2 3. ( ) 120 120 2. 3 120. 180... 2 180 2005. 2 1 0/5 120 ( ) 3 180. 120 2 3. 120. 1 2 2. 3. 180 120 0/5. 180. 61 1386
. GC/MS...[26] 1. Milan S. Spice antioxidants isolation and their antiradical activity. J. Food Compos. Anal. 2006; 19: 531-537. 2. Capecka E, Mareczek A and Leja M. Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chem. 2005; 93: 223-226. 3. Zheng W and Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chem. 2001; 49: 5165-5170. 4. Karpin A, ska M, Borowski J and Danowska- Oziewicz M. Use of natural antioxidants in readyto-serve food. Food Chem. 2001; 72: 5-9. 5. Ames BM. Dietary carcinogens and anticarcinogens: oxygen radical and degenerative diseases. Sci. 1983; 221: 1256-1263. 6. Frankel EN. Recent advances in lipid oxidation. J. Sci. Food Agric. 54: 495-511. 7. Gardner HW. Lipid hydroperoxide reactivity with proteins and amino acids. J. Agric. Food Chem. 1979; 27: 220-229. 8. Baardseth P. Effect of selected antioxidants on the stability of dehydrated mashed potatoes. Food Addit. Contam. 1989; 6: 201-207. 9. Lee SJ, Umano K, Shibamoto T and Lee KG. Identification of volatile components in basil (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chem. 2005; 91: 131-137. 10. Dormana HJD, Peltoketo A, Hiltunen R and Tikkanen MJ. Characterisation of the antioxidant properties of deodourised aqueous extracts from selected Lamiaceae herbs. Food Chem. 2003; 83: 255-262. 11. Omidbaigi R. Production and Processing of Medicinal Plants. Vol. 3. Astan Quds Publication. Tehran. 2000, pp: 397. 12. Che Man Y and Jaswir I. Effect of rosemary and sage extracts on frying performance of refined, bleached and deodorized (RBD) palm olein during deep-fat frying. Food Chem. 2000; 69: 301-307. 13. Demo A, Petrakis C, Kefalas P and Boskou D. Nutrient antioxidants in some herbs and Mediterranean plant leaves. Food Res. Int. 1998; 31: 351-354. 14. Tepe B, Sokmen M, Akpulat HA, Daferera D, Polissiou M and Sokmen A. Antioxidative activity of the essential oils of Thymus sipyleus subsp. sipyleus var. sipyleus and Thymus sipyleus subsp. sipyleus var. rosulans. J. Food Engineer. 2005; 66: 447-454. 15. Sokmen A, Gulluce M, Askin Akpulat H, Daferera D, Tepe T, Polissiou M, Sokmen M and Sahin F. The in vitro antimicrobial and antioxidant 62
... activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food Control. 2005; 15: 627-634. 16. Herodez SS, Hadolin M, Skerget M and Knez Z. Solvent extraction study of antioxidant from balm leaves. Food Chem. 2003; 80: 275-282. 17. Javanmardi J, Stushnoff C, Locke E and Vivanco JM. Antioxidant activity and total phenolic conent of Iranian occimum accessions. Food Chem. 83: 547-550. 18. Nedyalka V, Yanishlieva NV, Marinova EM, Gordon MH and Raneva VG. Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chem. 1999; 64: 59-66. 19. Lee KG and Shibamoto T. Antioxidant property of aroma extract isolated from clove buds (Syzygium aromaticum L.). Food Chem. 2001; 74: 443-448. 20. Ghasemi Dehkordi N (Ed.). Iranian Herbal Pharmacopoeia. Ministry of Health Publisher. Tehran. 2002, pp: 795. 21. Pokorny J, Yanishlieva N and Gordon M. Antioxidants in Food. CRC Press. 2001, pp: 70-80. 22. Mau JL, Lai EYC, Wang NP, Chen CC, Chang CH and Chyau CC. Composition and antioxidant activity of the essential oil from Curcuma zedoaria. Food Chem. 2003; 82: 583-591. 23. Sun T and Ho CT. Antioxidant activity of buckwheat extracts. Food Chem. 2005; 90: 743-749. 24. Miliauskas G, Venskutonis PR and Beek TAV. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004; 85: 231-237. 25. Xu J, Chen S and Hu Q. Antioxidant activity of brown pigment and extracts from black sesame seed (Sesamum indicum L.). Food Chem. 2005; 91: 79-83. 26. Tomaino A, Cimino F, Zimbalatti V, Venuti V, Sulfaro V, De Pasquale A and Saija A. Influence of heating on antioxidant activity and the chemical composition of some spice essential oils. Food Chem. 2005; 89: 549-554. 63 1386