Plant Tissue Culture S. S. Purohit AXIS BOOKS (INDIA)
Published by: AXIS BOOKS (INDIA) All Rights Reserved, 2010 Axis Books (India) ISBN No. (13): 978-93-80655-00-0 Price: Rs. 795.00 / US$ 40.00 Published by: Laser Typeset at: Cover Design by: Printed at:
Preface tissue culture plantlet culture micropropagation invitro Author
Contents 1 PLANT TISSUE CULTURE: AN OVERVIEW...1 Why Growth Occurs?... 3 Kinetics of Growth or Measurement and Pattern of Growth... 4 What Happens When Growth Occurs?... 4 Power of Regeneration in Plants... 5 Why Plants Regenerate?... 6 Callus Culture... 7 Meristem Culture... 7 Organ Culture... 7 Contrasts between Plant and Animal Cells... 8 2 HISTORY OF PLANT TISSUE AND CELL CULTURE...12 20 th Century Development...13 Knop and Prdifer developed synthetic media...13 Callus Culture of Carrots...14 F. C. Steward...15 H. E. Street...15 C. O. Miller...15 F. Skoog...16 Toshio Murashige...16 Vasil and Hilderbrandt...16 Tissue Culture Researches in India...22 Bioinformatics...25 Gene Banking, Conservation and Exchange in India...25
3 GENERAL METHODOLOGY OF PLANT TISSUE CULTURE...27 Cabinets or Shelves...27 Control of Bacterial and Fungal Contaminants by Antibiotics in Plant Tissue Culture...35 Procedure...37 Why Aeration is Essential?...38 Age of the Plant Tissue...39 General Procedure...39 Suspension Cell Culture from Callus in vitro...42 Nutrition of Callus Cultures...44 Inorganic Salts...45 Plant Growth Regulators...46 Vitamins...47 Carbon Source...47 Gelling Agent...47 Amino Acids and Amides...47 Antibiotics...48 Natural Complexes...48 Use and Storage of Coconut Water...50
Initiation of Embryogenic Tissue...54 Maturation of Embryos...57 Simplification of the Technique...57 Different Causes of Failures (and Remedies)...57 4 CULTURE MEDIA INGREDIENTS, PREPARATION AND RELATED PROBLEMS...59 Inorganic Salts...59 Guidelines for Maintaining Stock Solutions...62 Organic Chemicals...63 Growth Regulators...65 Other Growth Regulating Substances...67 Amino Acids...68 Antibiotics...68 Chemically Undefined Constitutes of Tissue Culture Media...69 Natural Complexes...71 Working with 32 P Labelled Compounds...72 Preparation of Dialysis Tubing...73 5 METHODS OF STERILIZATION AND DISINFESTATION...74 Sterilization by Heat...76 Thermal Death Point...76 Sterilization by Filtration...80 Laminar Flow Biological Safety Cabinets...80 Sterile Technique for Explants...82 Sterile Technique for Transfer...83 Chemical Disinfectants...85 Classification of Chemical Disinfectants...85 Testing for Solid-Surface Biological Contaminants...86
How to Observe Contaminants...87 6 ASEPTIC TECHNIQUES AND PREPARATION OF EXPLANTS...88 Cleaning (Preparation of Glasswares/ Plastic wares (Autoclavable)...89 Sterilization...89 Surfactants...89 Which Plant is to be selected for Explant?...91 How to Obtain Explants?...91 Cleaning and Treatment of Explant...92 7 HISTOLOGICAL AND PHOTOGRAPHIC TECHNIQUES FOR PLANT TISSUE CULTURE...94 General Considerations for Paraffin Studies...95 Immobilization of Specimens for Paraffin Sectioning... 100 Dissection and mounting of specimens... 101 Photographic Methods for Plant Cell and Tissue Culture... 103 Type of Photography for Plant Cell and Tissue Culture Research... 103 Macrophotography... 103 Microphotography with Stereomicroscopes... 104 Focusing and Cropping the Image... 105 Types of the Films... 105 Composition... 106 8 PROTOPLAST TECHNOLOGY...107 Enzymatic method... 108 Mechanical method... 109 Laboratory Facilities... 114 Enzyme Mixture and Osmotic Stabilizer (Osmotica)... 114 Sedimentation and Washing... 115 Flotation... 115 Other Purification Methods... 116
General Steps of Protoplast Culture... 116 Protoplast Culture Media for PC I Group... 117 Murashige and Skoog Medium... 118 Protoplast Culture Media PC II Group... 118 Protoplast Culture Media PC III Group... 119 Agar Embedded Culture... 120 Micro chambers... 120 Hanging Drop Cultures (HDC) Techniques... 120 Multidrop Array (MDA) Techniques... 120 Protoplast Culture: Regeneration of Cell Wall... 121 Development of Callus/Whole Plant... 123 General Steps of Protoplast Fusion... 126 Organogenesis... 129 Embryogenesis... 129 Handling of Regenerated Plantlets... 131 Incorporation of Foreign DNA... 132 Incorporation of Nuclei... 132 Incorporation of Chloroplasts... 132 Incorporation of Cyanobacteria... 133 Incorporation of Bacteria... 133 Incorporation of Virus... 133 Visual Selection... 134 Fluorescent Labels... 134 Fluorescence Activated Cells Sorting... 134 Nutritional Selection... 134 Drug Sensitivity and Resistance... 135 Plasmalemma... 136 Chloroplasts... 136 Mitochondria... 136 Vacuoles... 137
Plant Tissue Culture Publisher : Agrobios Publications ISBN : 9789380655000 Author : S S Purohit Type the URL : http://www.kopykitab.com/product/6931 Get this ebook