Place of Birth Date of Birth FACULTY POSITION: 2009-today 2004-2009 1999-2004 ZOI LYGEROU Associate Professor, School of Medicine, University of Patras, Greece Thessaloniki, Greece 06 / 01/ 1970 Associate Professor of Biology, School of Medicine, University of Patras, Greece Assistant Professor of Biology, School of Medicine, University of Patras, Greece Lecturer in Biology, School of Medicine, University of Patras, Greece POSTDOCTORAL RESEARCH: 1996-1999 Nov 1995- April 1996 EDUCATION: Imperial Cancer Research Fund, Cell Cycle Laboratory, Group Leader Paul Nurse. Project: "Cell Cycle Control and the regulation of DNA replication in fission yeast EMBL, Heidelberg, Gene Expression Program, Group Leader Bertrand Seraphin Project: "Identification and characterization of the human Pop1 protein: an autoantigenic protein subunit of RNase P and RNase MRP ribonucleoprotein complexes 1991-1995 1987-1991 AWARDS: PhD. EMBL Heidelberg, Gene Expression Program, Group: Iain Mattaj, Supervisor Bertrand Seraphin. Project: Studies of the composition, biogenesis and function of snrnp particles in the yeast Saccharomyces cerevisiae PhD (Dr Rer Nat) awarded by the University of Heidelberg (Magna cum laude) Diploma. Department of Biology, University of Athens, Greece. Grade Excellent 1990-1991: Diploma Research, National Hellenic Research Foundation, Athens, Greece SET-Routes University Ambassador (2007-) EMBO Young Investigator 2001 Awards from the State Scholarship Foundation for every year of University Studies (1987-1991) RESEARCH ACTIVITIES: PI of a team studying cell cycle control, currently numbering 9 members. We study the control mechanisms which safeguard genomic stability by ensuring once per cell cycle replication in eukaryotic cells and how defects in this control may lead to tumorigenesis. To this end, we combine: molecular-cell biology studies in human cells, genetic studies in fission yeast, analyses of tumour specimens, advanced live cell imaging and modelling of biological networks. OTHER RESEARCH RELATED ACTIVITIES: Head of the Confocal Microscopy Facility of the University of Patras (co-head: S. Taraviras) Organization of common facilities at Patras Medical School (securing funding, operation schemes etc) Reviewer of Research Grants for the Medical Research Council-UK, Association for International Cancer Research, Portuguese Research Ministry, Greek Ministry of Education and EMBO fellowships Reviewer for the Journals Development, J Cell Biol, Mol Biol Cell, Nucl Acid Res, J Cell Sci, Trends Cell Biol, Br J Cancer, Mol Carcinogen, Cancer Letters, PlosOne Editor for Frontiers in Biosciences Symposia Organization: 1 st and 2 nd Cell Cycle Mini-Symposium (2001-Patras, 2005-Athens); Biotechnology Colloquiuum 2009-Patras; Advanced Light Microscopy Symposium 2010-Patras >35 invited lectures at Research Institutes and Universities in Europe and the USA (eg NIMR-MRC, CR-UK, U-Oxford, U-Sussex, NKI-NL, EMBL, Helmholtz Munich, U-Copenhagen, U-Geneva, Rockefeller, Tufts) International collaborations: Paul Nurse UK-CMRI, P. Bastiaens MPI-Dortmund, E. Stelzer Uni-Frankfurt, J. Lygeros ETH-Zurich, H. Nishitani -Japan, A. Perrakis-NKI 1
TEACHING ACTIVITIES Supervisor of 8 PhD Theses, 19 MSc Theses and 4 Undergraduate Diploma Theses Member in over 20 PhD Examination Committees and over 30 MSc Examination Committees (other than as supervisor) in Greece and abroad. Postgraduate teaching in 3 MSc/PhD Programs at the University of Patras; Co-ordinator of the postgraduate courses Computational methods for genomic and proteomic analysis, Molecular Biology-Molecular Medicine, Research Methodology, Current Literature Analysis ; Active involvement in the organization of a new Interdisciplinary MSc Program in Life Sciences Informatics; Participation as invited lecturer to Postgraduate Programs run by the University of Athens and Crete, to courses to Medical Doctors under specialization and to High School Biology Teachers. Undergraduate teaching: First and second semester Biology teaching to Medical Students at Uni-Patras Participation in the translation to Greek of Genes VIII (Lewin) and The Cell: a molecular approach (Cooper) Active involvement in introducing innovative teaching methods at pregraduate and postgraduate level at Patras Medical School (small group teaching, problem based learning, e-learning, complementary skills) Coordinator of European Student Exchange and Credit Transfer for Patras Medical School (ECTS, 2004-2006) Organization of training activities for new technologies (Live-cell imaging, Microarray analysis, Modeling) RESEARCH GRANTS (Primary Investigator) International Grants: ERC-2011-StG, Project DYNACOM, From Genome Integrity to Genome Plasticity: Dynamic Complexes Controlling Once Per Cell Cycle Replication, 2012-2017 FP7-REGPOT-2011-1. Developing research potential grant SEE-DRUG, 2011-2014 Association for International Cancer Research. Research Grant 2009-2012 Title: Dynamic Complexes Maintaining Genome Integrity HYGEIA (FP6-NEST-STREP-04995). 2005-2007 Title: Hybrid Systems for Biochemical Network Modeling and Analysis Collaborating teams: ETH-Zurich (J. Lygeros, Coordinator), UPATRAS (Z., S.Taraviras), INRIA (H. de Jong), EMBL (J. Wittbrodt), Pavia (G. Ferrari-Trecate), Rockefeller (P. Nurse). HFSP Young Investigator Grant 2002-2005 Title: Controlling the onset of S-phase: interactions at the heart of origin licensing Collaborative team: Z. (CoOrdinator), P. Bastiaens (EMBL), H. Nishitani (Kyoto) EMBO Young Investigator Award 2001-2004 Title: Control over S-phase onset: from yeast to human cells. Association for International Cancer Research. Research Grant 2000-2003 Title: Regulation of S-Phase onset in fission yeast and human cells. National Grants: Heracletus II. 2010-2013. Support for two PhD fellows from the Greek Ministry of Education. General Secretariat of Research and Technology, 2005-2007 Matching Funds for Program HYGEIA Pythagoras ΙΙ Research Grant (Greek Ministry of Education), 2005-2007 Title: The role of the cell cycle regulator Geminin during cellular senescence Program K. Karatheodori 2005, University of Patras, 2005-2008 Title: The licensing regulator Cdt1 and the DNA damage response Program K. Karatheodori 2000, University of Patras, 2000-2003 Title: The cell cycle regulator Cdt1: molecular mechanism of function and clinical applications. Empeirikio Foundation (Greek Charity), Research Award 2001 Title: Studies of HsCdt1 in normal and cancer cells. 2
Publication Record: Number of peer-reviewed scientific articles 35 Original Research Articles 23 Number of articles where first author 10 Number of articles where corresponding author 11 Scientific articles within the last 5 years 15 Original articles within the last 5 years 11 Mean journal impact factor (all original articles) 9 Total Citations >1400 h-index 18 Number of presentations at scientific meetings >80 Invited lectures at scientific meetings >15 Invited seminars >35 Selected Publications: 1. Spella M, Kyrousi C, Kritikou E, Stathopoulou A, Guillemot F, Koussis D, Pachnis, Z, Taraviras S (2011). Geminin regulates cortical progenitor proliferation and differentiation. Stem Cells, 29:1269-1282 2. Pefani DE, Dimaki M, Spella M, Karantzelis N, Mitsiki E, Kyrousi C, Symeonidou IE, Perrakis A, Taraviras S, Z. (2011). Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression. J. Biol. Chem, 286:23234-46 3. Roukos V, Kinkhabwala A, Colombelli J, Kotsantis P, Taraviras S, Nishitani H, Stelzer E, Bastiaens P, and Z (2011). Dynamic Recruitment of the Licensing Factor Cdt1 to Sites of DNA Damage J. Cell Science, 124:422-432 4. J. Lygeros, K. Koutroumpas, S. Dimopoulos, I. Legouras, P. Kouretas, C. Heichinger, P. Nurse, Z. (2008) Stochastic hybrid modelling of DNA replication across a complete genome. Proc Natl Acad Sci USA, 105:12295-12300. 5. G. Xouri, A. Squire, M. Dimaki, B. Geverts, P. J. Verveer, S. Taraviras, H. Nishitani, A. B. Houtsmuller, P.I. H. Bastiaens, Z..(2007) Dynamic chromatin association of Cdt1 throughout G1 leads to Geminin recruitment onto chromatin. EMBO J, 26:1303-1314. 6. V. Roukos, M. S. Iliou, H. Nishitani, M. Gentzel, M. Wilm, S. Taraviras, Z. (2007) Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit Brahma. J. Biol. Chem, 282:9346-9357 7. Nishitani H, Sugimoto N, Roukos V, Nakanishi Y, Saijo M, Obuse C, Tsurimoto T, Nakayama KI, Nakayama K, Fujita M, Z, Nishimoto T. (2006). Two E3 ubiquitin ligases, SCF-Skp2 and DDB1- Cul4, target human Cdt1 for proteolysis. EMBO J. 25(5):1126-36 8. V. Roukos, M. S. Iliou, H. Nishitani, M. Gentzel, M. Wilm, S. Taraviras, Z. (2007) Geminin cleavage during apoptosis by caspase-3 alters its binding ability to the SWI/SNF subunit Brahma. J. Biol. Chem, 282:9346-9357 9. Karakaidos, P., S. Taraviras, L. Vassiliou, P. Zacharatos, N. Kastrinakis, D. Kougiou, M. Kouloukoussa, H. Nishitani, A. Papavasileiou, Z. c and V. Gorgoulis c (2004). Overexpression of the replication licensing regulators hcdt1 and hcdc6 characterizes a subset of Non-Small-Cell Lung Carcinomas. American J Pathol, 165 (4): 1351-1365 c corresponding authors 10., Z and P. Nurse (2000) License withheld: blocking DNA replication onset by Geminin. Science, 290: 2271-2273 (Perspective) 11. Nishitani, H.,, Z.*, Nishimoto, T. and P. Nurse (2000) The Cdt1 protein is required to license DNA for replication in fission yeast Nature, 404:625-628 *equally contributing first author and corresponding author 12., Z., H. Pluk., W.J. van Venrooij and B. Séraphin (1996). hpop1: an autoantigenic protein subunit shared by the human RNase P and RNase MRP ribonucleoproteins. EMBO J., 15:5936-5948 13., Z., C. Allmang, D. Tollervey and B. Séraphin (1996).Accurate processing of a eukaryotic precursor ribosomal RNA by ribonuclease MRP in vitro. Science, 272: 268-270 3
Μέγιστο 2 σελίδες Ερευνητική Ταυτότητα Μέλους ΟΝΟΜΑΤΕΠΩΝΥΜΟ ΘΕΟΦΙΛΟΣ ΜΑΝΔΑΜΑΔΙΩΤΗΣ ΤΙΤΛΟΣ ΠΑΡΟΥΣΑΣ ΘΕΣΗΣ ΕΠΙΚ. ΚΑΘΗΓΗΤΗΣ Πεδίο Ερευνητικής Δραστηριότητας: ΒΙΟΓΡΑΦΙΚΑ ΣΤΟΙΧΕΙΑ ΚΥΡΙΟΥ ΕΡΕΥΝΗΤΗ ΕΚΠΑΙΔΕΥΣΗ ( Από το πρώτο πτυχίο μέχρι τις μεταδιδακτορικές σπουδές.) ΠΤΥΧΙΟ Ονομασία και τοποθεσία Ιδρύματος (εάν απονεμήθηκε) ΕΤΟΣ/ΕΤΗ ΠΕΔΙΟ ΣΠΟΥΔΩΝ Royal Melbourne Institute of Technology, Μελβούρνη B.App.Science 1985 Βιοχημεία Monash University, Μελβούρνη, Αυστραλία B.Sci. (Hons) 1989 Βιοχημεία Πανεπιστήμιο Μελβούρνη, Μελβούρνη, Αυστραλία Ph.D. 1993 Μοριακή Βιολογία ΘΕΣΕΙΣ ΕΡΓΑΣΙΑΣ Ονομασία και τοποθεσία Οργανισμού ΘΕΣΗ ΕΤΟΣ/ΕΤΗ Ludwig Inst Cancer Research, Μελβούρνη, Αυστραλία Post-doctoral research fellow 1 Deutsches Krebsforschungszentrum, Χαïδελβέργη Research Fellow 5 Peter MacCallum Cancer Centre, Μελβούρνη Senior Research Officer 5 Monash University, Μελβούρνη Senior Lecturer 3 ΤΙΜΕΣ και ΒΡΑΒΕΙΑ ΔΗΜΟΣΙΕΥΣΕΙΣ (σε διεθνή περιοδικά με κριτές) 33 ΑΝΑΦΟΡΕΣ: 894 Δείκτης h: 13 ΕΡΕΥΝΗΤΙΚΕΣ ΔΡΑΣΤΗΡΙΟΤΗΤΕΣ (στα πλαίσια του δικτύου) Ανάπτυξη και μελέτη ζωϊκών και κυτταρικών μοντέλων Τεχνολογίες μοριακής/κυτταρικής ανάλυσης Γονιδιωματική ανάλυση ΜΕΛΗ ΕΡΕΥΝΗΤΙΚΗΣ ΟΜΑΔΑΣ ΕΠΙΛΕΓΜΕΝΕΣ ΠΡΟΣΦΑΤΕΣ ΔΗΜΟΣΙΕΥΣΕΙΣ ΣΕ ΔΙΕΘΝΗ ΠΕΡΙΟΔΙΚΑ ΜΕ ΚΡΙΤΕΣ Darido C, Buchert M, Pannequin J, Bastide P, Zalzali H, Mantamadiotis T, Bourgaux JF, Garambois V, Jay P, Blache P, Joubert D, Hollande F. (2008) Defective claudin-7 regulation by Tcf-4 and Sox-9 disrupts the polarity and increases the tumorigenicity of colorectal cancer cells. Cancer Res., 68(11):4258-68 Malaterre J, *Mantamadiotis T, Dworkin S, Lightowler S., Nichols NR, Ransome MI, Turnley AM, Emambokus N, Frampton J, Ramsay RG. (2007) c-myb is required for neural progenitor cell proliferation and maintenance of the neural stem cell niche in adult brain. Stem Cells, 26(1):173-81 *Equal First and Corresponding Author Dworkin,S., Heath,J.K., Malaterre,J., Hogan,B.M., DeJong-Curtain,T., Ramsay,R.G., Mantamadiotis,T.. (2007) CREB regulates neural proliferation, midbrain-hindbrain organization and patterning in zebrafish. Dev. Biol. 147(2):373-87 Malaterre,J., Ramsay,R.G. and *Mantamadiotis,T., (2007) Wnt-frizzled signaling and the many paths to neural development and adult brain homeostasis. Frontiers in Bioscience, 12: 492-506. Mantamadiotis,T., Kretz,O., Ridder,S., Bleckmann,S.C., Bock,D., Gröne,H.-J., Malaterre,J., Dworkin,S., Ramsay,R.G., Schütz,G. (2006) Hypothalamic CREB loss causes anterior pituitary hypoplasia and dwarfism in mice. Molecular Endocrinology 20(1):204-211 Mantamadiotis,T., Lemberger,T., Bleckmann,SC., Kern,H., Kretz,O., Martin-Villalba,A., Tronche,F., Kellendonk,C, Gau,D., Kapfhammer,J., Otto,C., Schmid,W., Schütz,G. (2002) Disruption of CREB function in brain leads to neurodegeneration. Nature Genetics 31(1): 47-54
ΧΡΗΜΑΤΟΔΟΤΗΣΕΙΣ (ενδεικτικές πρόσφατες χρηματοδ., Ερευνητικά Πρωτόκολλα), ΠΑΤΕΝΤΕΣ, ΠΑΡΟΧΗ ΥΠΗΡΕΣΙΩΝ 2009-2012 EU Marie Curie Reintegration Grant 100.000 (Mantamadiotis) 2009-2011 Australian Research Council Discovery Grant $AUD360.000 (Porter, Scanlon, Mantamadiotis) 2002-2004 National Health & Medical Research Council $AUD255.000 (Mantamadiotis, Vogiagis, Ramsay) ΣΥΝΕΡΓΑΣΙΕΣ (ΔΙΕΘΝΕΙΣ-ΕΘΝΙΚΕΣ) Prof. Jon Frampton, Lab of Stem Cell Biology, Institute of Biomedical Research, University of Birmingham, UK Dr. Frédéric Hollande, Institut de Génomique Fonctionnelle, Département d'oncologie Cellulaire et Moléculaire, Montpellier, France Prof. Guenther Schuetz, Deutsches Krebsforschungszentrum, Heidelberg, Germany Dr. Stavros Taraviras, Medical School, University of Patras, Greece ΔΙΟΡΓΑΝΩΣΗ ΣΥΝΕΔΡΙΩΝ / ΘΕΡΙΝΩΝ ΣΧΟΛΕΙΩΝ ΥΠΟΣΤΗΡΙΞΗ ΜΕΤΑΠΤΥΧΙΑΚΩΝ ΠΡΟΓΡΑΜΜΑΤΩΝ ΣΠΟΥΔΩΝ / ΔΙΔΑΚΤΟΡΙΚΑ PhD Dr. Honor Hugo, 2005, University of Melbourne PhD Dr. Sebastian Dworkin, 2008, University of Melbourne PhD Dr. Ben Williams, 2009, University of Melbourne ΣΥΝΕΡΓΑΣΙΕΣ ΜΕ ΜΕΛΗ ΤΟΥ ΔΙΚΤΥΟΥ (κοινές δημοσιεύσεις, χρηματοδοτήσεις, διδακτορικά, Master, οργάνωση συνεδρίων, ερευνητικά πρωτόκολλα) Spella,M., Britz,O., Kotandaki,P.,,Z., Nishitani,H., Ramsay,R.G., Flordellis,C., Guillemot,F., Matamadiotis,T., Taraviras,S. (2007) Licensing regulators Geminin and Cdt1 identify progenitor cells of the mouse CNS in a specific phase of the cell cycle. Neuroscience 307(1):127-41 Taraviras,S., Mantamadiotis,T., Dong-Si,T., Mincheva,A., Lichter,P., Drewes,T., Ryffel,GU., Monaghan,AP., Schütz,G. (2000) Primary structure, chromosomal mapping, expression and transcriptional activity of the murine Hepatocyte Nuclear Factor 4γ (mhnf4γ) gene. Biochim. Biophys. Acta Gene Struct Expression 1490: 21-32 Mantamadiotis,T., Taraviras,S., Tronche,F., Schütz,G. (1998) PCR-based strategy for genotyping mice and ES cells harboring loxp sites. Biotechniques 25: 968-972