Development of Methods for the Specific Enrichment of Glycopeptides for the LC/MS Analysis

Μέγεθος: px
Εμφάνιση ξεκινά από τη σελίδα:

Download "Development of Methods for the Specific Enrichment of Glycopeptides for the LC/MS Analysis"

Transcript

1 LC/MS Development of Methods for the Specific Enrichment of Glycopeptides for the LC/MS Analysis Masahiro Yodoshi, Tomoko Ikuta, Yukie Mohri, Takehiro Oyama, Shigeo Suzuki School of Pharmacy, Kinki University, 3 4 1, Kowakae, Higashi osaka, , Japan. Abstract Glycosylation affects various biological functions of proteins, and it also recognized as an indicator of disease. Recent glycoproteomics studies require methods capable to enrich glycopeptides from protease digests, because MS sensitivity of glycopeptides is much lower than that of peptides. In addition salts or detergents added in the course of sample preparation impair ionization efficiency of glycoconjugates. Therefore, many researchers have been surveyed the methods to enrich glycoproteins and glycopeptides from crude samples such as urine, plasma and cells. Here we overview recent progresses of the enrichment methods of glycopeptides and glycans applied for the determination of glycosylation sites, structural analysis of carbohydrates, and their applications to glycomics or glycoproteomics studies. After that we introduced our recent studies on three novel methods for the enrichment of glycoconjugates; serotonin immobilized silica SPE for trapping sialic acid containing glycoconjugates, borate form anion exchanger to trap all of glycoconjugates, and various lectins and centrifugal ultrafiltration membrane for specific recovery of glycoconjugates based on the specificities of lectins to glycan structures. Keywords: solid phase extraction, glycoproteomics, MS, enrichment, serotonin bonded silica, borate form anion exchanger, lectin. [1] [2] [3, 4] microheterogeneity [5 7] School of Pharmacy, Kinki University, 3 4 1, Kowakae, Higashi osaka, , Japan. Correspondence autor : Shigeo Suzuki Tel : Fax : E mail : suzuki@phar.kindai.ac.jp

2 [7 11] LC MALDI MS LC ESI MS LC /ESI MS [12 15] [16 21] LC MS MS n [22] LC/MS SPE Scheme 1 [23] Scheme 1. Compexation between boric acid or phenylboronic acid and saccharides. OH [24 27] [28] [29] [30] [31] SPE SPE Hypercarb TFA

3 MS [32 38] Packer SPE [39] [40] MALDI MS [41] SPE hydrophilic interaction chromatography; HILIC [42 44] HILIC [18, 45 47] HILIC LC/ESI MS [48 50] Takegawa Deguchi zwitterionic HILIC ZIC HILIC [51 54] HILIC Shimizu N N [55] SPE MALDI MS MS n [56, 57] v/v v/v Yu SPE MALDI MS [58] [47, 57] [59] [60] Con ALCA Con A LCA [61 63] [64] [65] MALDI MS LC/ESI MS [28] [66, 67] [68, 69] Hirabayashi [70 72] [73] [74] MAM

4 SSA 5HT N NeuAc Sturgeon [75] µm nm γ 5HT 5HT Scheme 2 [65] SPE Fig. 1 NeuAc M AP Fig. 2 Fig. 2. Reversed phase HPLC analysis of AP labeled glycans derived from human transferrin before (a) and after (b) specific extraction with Si 5HT SPE cartridge. Analytical conditions: column, ODS silica column ( mm, Nacalai Tesque); buffer, (A)10 mm NaH2PO4 (ph 3.8), (B)buffer A containing 0.5% 1 BuOH; eluent, 70% to 5% of buffer A for 100 min; flow rate, 1.0 ml/min; detection, fluorescence at 310 (ex)/390 (em) nm. Scheme 2. Synthesis of the serotonin immobilized silica. Fig. 1. Procedure for purification of sialylated glycan derivatives and/or peptides using Si 5HT SPE. Scheme 1 SPE ph B RNase B Fig. 3 LC/MS TIC Fig. 3(a) SPE

5 Fig. 3. Total ion current chromatograms of reversed phase LC/ESI MS of tryptic digests of ribonuclease B before (a) and after (b) specific extraction with borate form anion exchange resins. MS scan range: m/z. GlcNac; N acethyl glucosamine, Man; Mannose. Fig. 4. Total ion current chromatograms of reversed phase LC/ESI MS of tryptic digests of ribonuclease B before (a) and after (b) specific extraction with Con A. MS scan range: m/z. GlcNac; N acethyl glucosamine, Man; Mannose. Fig. 3(b) SPE µg MNaCl mm CaCl mm MnCl mm MgCl M Tris HCl ph µg TFA ml Fig. 4 RNase B Con A α SSA SPE SPE

6 Reference [1] Apweiler, R.; Hermjakob, H.; Sharon, N., Biochim. Biophys. Acta, 1999, 1473, 4 8. [2] Takahashi, N.; Kato, K., Trends in Glycosci. Glycotech., 2003, 15, [3] Moloney, D. J.; Panin, V. M.; Johnston, S. H.; Chen, J.; Shao, L.; Wilson, R.; Wang, Y.; Stanley, P.; Irvine, K. D.; Haltiwanger, R. S.; Vogt, T. F., Nature, 2000, 406, [4] Lehle, L.; Strahl, S.; Tanner, W., Angew. Chem. Int. Ed., 2006, 45, [5] Varki, A., Glycobiology, 1993, 3, [6] Dwek, R. A., Chem. Rev., 1996, 96, [7] Rudd, P. M.; Dwek, R. A., Crit. Rev. Biochem. Mol. Biol., 1997, 32, [8] Banks, R. E.; Dunn, M. J.; Hochstrasser, D. F.; Sanchez, J. C.; Blackstock, W.; Pappin, D. J.; Selby, P. J., Lancet, 2000, 356, [9] Harvey, D. J., Proteomics, 2001, 1, [10] Kuster, B.; Krogh, T. N.; Mortz, E.; Harvey, D. J., Proteomics, 2001, 1, [11] Spiro, R. G., Glycobiology, 2002, 12, 43R 56R. [12] Kawasaki, N.; Ohta, M.; Itoh, S.; Hyuga, M.; Hyuga, S.; Hayakawa, T., Biologicals, 2002, 30, [13] Karlsson, N. G.; Schulz, B. L.; Packer, N. H., J. Am. Soc. Mass Spectrom., 2004, 15, [14] Kremmer, T.; Szollosi, E.; Boldizsar, M.; Vincze, B.; Ludanyi, K.; Imre, T.; Schlosser, G.; Vekey, K., Biomed. Chromatogr., 2004, 18, [15] Zamfir, A.; Seidler, D. G.; Schonherr, E.; Kresse, H.; Peter Katalinic, J., Electrophoresis, 2004, 25, [16] Harvey, D. J., Expert Rev. Proteomics, 2005, 2, [17] Kawasaki, N.; Itoh, S.; Harazono, A.; Hashii, N.; Matsuishi, Y.; Hayakawa, T.; Kawanishi, T., Trends in Glycosci. Glycotech., 2005, 17, [18] Wuhrer, M.; Deelder, A. M.; Hokke, C. H., J. Chromatogr. B, 2005, 825, [19] Geyer, H.; Geyer, R., Biochim. Biophys. Acta, 2006, 1764, [20] Kaji, H.; Isobe, T., Trends in Glycosci. Glycotech., 2006, 18, [21] Wuhrer, M.; Catalina, M. I.; Deelder, A. M.; Hokke, C. H., J. Chromatogr. B, 2007, 849, [22] Annesley, T. M., Clin. Chem., 2003, 49, [23] Hageman, J. H.; Kuehn, G. D., Methods Mol. Biol., 1992, 11, [24] Gould, B. J.; Hall, P. M.; Cook, J. G., Ann. Clin. Biochem., 1984, 21 (Pt 1), [25] Rendell, M.; Rasbold, K.; Nierenberg, J.; Krohn, R.; Hermanson, G.; Klenk, D.; Smith, P. K., Clin. Biochem., 1986, 19, [26] Danze, P. M.; Tarjoman, A.; Rousseaux, J.; Fossati, P.; Dautrevaux, M., Clin. Chim. Acta, 1987, 166, [27] Gould, B. J.; Hall, P. M., Clin. Chim. Acta, 1987, 163, [28] Sparbier, K.; Koch, S.; Kessler, I.; Wenzel, T.; Kostrzewa, M., J. Biomol. Tech., 2005, 16, [29] Zhang, Q.; Tang, N.; Brock, J. W.; Mottaz, H. M.; Ames, J. M.; Baynes, J. W.; Smith, R. D.; Metz, T. O., J. Proteome. Res., 2007, 6, [30] Xu, Y.; Wu, Z.; Zhang, L.; Lu, H.; Yang, P.; Webley, P. A.; Zhao, D., Anal. Chem., 2009, 81, [31] Monzo, A.; Bonn, G. K.; Guttman, A., Anal. Bioanal. Chem., 2007, 389, [32] Davies, M.; Smith, K. D.; Harbin, A. M.; Hounsell, E. F., J. Chromatogr., 1992, 609, [33] Fan, J. Q.; Kondo, A.; Kato, I.; Lee, Y. C., Anal. Biochem., 1994, 219, [34] Koizumi, K., J. Chromatogr. A, 1996, 720, [35] Kawasaki, N.; Ohta, M.; Hyuga, S.; Hashimoto, O.; Hayakawa, T., Anal. Biochem., 1999, 269, [36] Itoh, S.; Kawasaki, N.; Ohta, M.; Hyuga, M.; Hyuga, S.; Hayakawa, T., J. Chromatogr. A, 2002, 968, [37] Hashii, N.; Kawasaki, N.; Itoh, S.; Hyuga, M.; Kawanishi, T.; Hayakawa, T., Proteomics, 2005, 5, [38] Itoh, S.; Kawasaki, N.; Hashii, N.; Harazono, A.; Matsuishi, Y.; Hayakawa, T.; Kawanishi, T., J. Chromatogr. A, 2006, 1103, [39] Packer, N. H.; Lawson, M. A.; Jardine, D. R.; Redmond, J. W., Glycoconj. J., 1998, 15, [40] Nakano, M.; Kakehi, K.; Lee, Y. C., J. Chromatogr. A, 2003, 1005, [41] Larsen, M. R.; Hojrup, P.; Roepstorff, P., Mol. Cell. Proteomics, 2005, 4, [42] Alpert, A. J., J Chromatogr, 1990, 499, [43] Alpert, A. J.; Shukla, M.; Shukla, A. K.; Zieske, L. R.; Yuen, S. W.; Ferguson, M. A.; Mehlert, A.; Pauly, M.; Orlando, R., J. Chromatogr. A, 1994, 676, [44] Boersema, P. J.; Mohammed, S.; Heck, A. J., Anal. Bioanal.

7 Chem., 2008, 391, [45] Hagglund, P.; Bunkenborg, J.; Elortza, F.; Jensen, O. N.; Roepstorff, P., J. Proteome. Res., 2004, 3, [46] Wuhrer, M.; Koeleman, C. A.; Hokke, C. H.; Deelder, A. M., Anal. Chem., 2005, 77, [47] Ruhaak, L. R.; Huhn, C.; Waterreus, W. J.; de Boer, A. R.; Neususs, C.; Hokke, C. H.; Deelder, A. M.; Wuhrer, M., Anal. Chem., 2008, 80, [48] Grumbach, E. S.; Wagrowski Diehl, D. M.; Mazzeo, J. R.; Alden, B.; Iraneta, P. C., LC GC North America 2004, 22, [49] Shou, W. Z.; Naidong, W., J. Chromatogr. B, 2005, 825, [50] Hemstrom, P.; Irgum, K., J. Sep. Sci., 2006, 29, [51] Takegawa, Y.; Deguchi, K.; Ito, H.; Keira, T.; Nakagawa, H.; Nishimura, S., J. Sep. Sci., 2006, 29, [52] Takegawa, Y.; Deguchi, K.; Keira, T.; Ito, H.; Nakagawa, H.; Nishimura, S., J. Chromatogr. A, 2006, 1113, [53] Deguchi, K., Chromatography, 2008, 29, [54] Deguchi, K.; Keira, T.; Yamada, K.; Ito, H.; Takegawa, Y.; Nakagawa, H.; Nishimura, S., J. Chromatogr. A, 2008, 1189, [55] Shimizu, Y.; Nakata, M.; Kuroda, Y.; Tsutsumi, F.; Kojima, N.; Mizuochi, T., Carbohydr. Res., 2001, 332, [56] Wada, Y.; Tajiri, M.; Yoshida, S., Anal. Chem., 2004, 76, [57] Tajiri, M.; Yoshida, S.; Wada, Y., Glycobiology, 2005, 15, [58] Yu, Y. Q.; Gilar, M.; Kaska, J.; Gebler, J. C., Rapid Commun Mass Spectrom., 2005, 19, [59] Boyd, W. C.; Reguera, R. M., J. Immunol., 1949, 62, [60] Goldstein, I. J.; Hughes, R. C.; Monsigny, M.; Osawa, T.; Sharon, N., Nature, 1980, 285, 66. [61] Ogata, S.; Muramatsu, T.; Kobata, A., J. Biochem, 1975, 78, [62] Krusius, T.; Finne, J.; H., R., FEBS Lett., 1976, 71, [63] Kornfeld, K.; Reitman, M. L.; Kornfeld, R., J. Biol. Chem., 1981, 256, [64] Cummings, R. D.; Kornfeld, S., J. Biol. Chem., 1982, 257, [65] Larsson, P. O., Methods Enzymol., 1984, 104, [66] Madera, M.; Mechref, Y.; Novotny, M. V., Anal. Chem., 2005, 77, [67] Madera, M.; Mechref, Y.; Klouckova, I.; Novotny, M. V., J. Chromatogr. B, 2007, 845, [68] Yang, Z.; Hancock, W. S., J. Chromatogr. A, 2004, 1053, [69] Yang, Z.; Hancock, W. S., J. Chromatogr. A, 2005, 1070, [70] Hirabayashi, J.; Arata, Y.; Kasai, K., Proteomics, 2001, 1, [71] Hirabayashi, J.; Hayama, K.; Kaji, H.; Isobe, T.; Kasai, K., J. Biochem., 2002, 132, [72] Hirabayashi, J.; Kasai, K., J. Chromatogr. B, 2002, 771, [73] Hirabayashi, J., Glycoconj. J., 2004, 21, [74] Mechref, Y.; Madera, M.; Novotny, M. V., Methods Mol. Biol., 2008, 424, [75] Sturgeon, R. J.; Sturgeon, C. M., Carbohydr. Res., 1982, 103,

Analysis of monosaccharides in the saffron corm glycoconjugate by capillary electrophoresis

Analysis of monosaccharides in the saffron corm glycoconjugate by capillary electrophoresis 2012 3 Vol. 30 No. 3 March 2012 Chinese Journal of Chromatography 304 ~ 308 DOI 10. 3724 /SP. J. 1123. 2011. 11015 * 210009 9. 5% v /v 80 2 h 234 nm 60 cm 50 cm 50 μm 25 20 kv 350 mmol /L ph 10. 21 3.

Διαβάστε περισσότερα

- A. A Biphenol A BPA BPA BPA LLE 5 SPE Electrospinning Kang. Packed-fiber solid-phase extraction PFSPE 1 ml

- A. A Biphenol A BPA BPA BPA LLE 5 SPE Electrospinning Kang. Packed-fiber solid-phase extraction PFSPE 1 ml 38 200 4 FENXI HUAXUE Chinese Journal of Analytical Chemistry 4 503 ~ 507 DOI 0. 3724 /SP. J. 096. 200. 00503 6 - A * 2 2 * 2 20009 2 20096 6 - A ph 6 0 ml ph 8. 0 3 ml /min. 5 mg 6 300 μl A 6 0. 20 ~

Διαβάστε περισσότερα

Free Radical Initiated Coupling Reaction of Alcohols and. Alkynes: not C-O but C-C Bond Formation. Context. General information 2. Typical procedure 2

Free Radical Initiated Coupling Reaction of Alcohols and. Alkynes: not C-O but C-C Bond Formation. Context. General information 2. Typical procedure 2 Free Radical Initiated Coupling Reaction of Alcohols and Alkynes: not C-O but C-C Bond Formation Zhongquan Liu,* Liang Sun, Jianguo Wang, Jie Han, Yankai Zhao, Bo Zhou Institute of Organic Chemistry, Gannan

Διαβάστε περισσότερα

Copper-catalyzed formal O-H insertion reaction of α-diazo-1,3-dicarb- onyl compounds to carboxylic acids with the assistance of isocyanide

Copper-catalyzed formal O-H insertion reaction of α-diazo-1,3-dicarb- onyl compounds to carboxylic acids with the assistance of isocyanide Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 Copper-catalyzed formal O-H insertion reaction of α-diazo-1,3-dicarb- onyl compounds to carboxylic

Διαβάστε περισσότερα

LUO, Hong2Qun LIU, Shao2Pu Ξ LI, Nian2Bing

LUO, Hong2Qun LIU, Shao2Pu Ξ LI, Nian2Bing 2003 61 3, 435 439 ACTA CHIMICA SINICA Vol 61, 2003 No 3, 435 439 2 ΞΞ ( 400715), 2, 2, 2, 3/ 2 2,, 2,, Ne w Methods for the Determination of the Inclusion Constant between Procaine Hydrochloride and 2Cyclodextrin

Διαβάστε περισσότερα

Supporting Information. Asymmetric Binary-acid Catalysis with Chiral. Phosphoric Acid and MgF 2 : Catalytic

Supporting Information. Asymmetric Binary-acid Catalysis with Chiral. Phosphoric Acid and MgF 2 : Catalytic Supporting Information Asymmetric Binary-acid Catalysis with Chiral Phosphoric Acid and MgF 2 : Catalytic Enantioselective Friedel-Crafts Reactions of β,γ- Unsaturated-α-Ketoesters Jian Lv, Xin Li, Long

Διαβάστε περισσότερα

8Q5SAC) 8Q5SAC UV2Vis 8500 ( ) ; PHS23C ) ;721 ( ) :1 4. ;8Q5SAC : molπl ;Britton2Robinson Q5SAC BSA Britton2Robinson,

8Q5SAC) 8Q5SAC UV2Vis 8500 ( ) ; PHS23C ) ;721 ( ) :1 4. ;8Q5SAC : molπl ;Britton2Robinson Q5SAC BSA Britton2Robinson, 31 2003 8 (FENXI HUAXUE) 8 Chinese Journal of Analytical Chemistry 976 980 22( 82 252 272 )21,82 23,62 3 (, 510631) 22(82 252 272 )21,82 23,62 (BSA), 8Q5SAC BSA 298K, 35 40 6. 1 10 5 LΠmol 8Q5SAC, ph =

Διαβάστε περισσότερα

Metal-free Oxidative Coupling of Amines with Sodium Sulfinates: A Mild Access to Sulfonamides

Metal-free Oxidative Coupling of Amines with Sodium Sulfinates: A Mild Access to Sulfonamides Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Supporting information for Metal-free Oxidative Coupling of Amines with Sodium Sulfinates:

Διαβάστε περισσότερα

Separation and Determination of Ephedrine Pseudoephedrine and Methylephedrine by Capillary Electrophoresis with Electrochemiluminescence Detection

Separation and Determination of Ephedrine Pseudoephedrine and Methylephedrine by Capillary Electrophoresis with Electrochemiluminescence Detection 31 2 2012 2 FENXI CESHI XUEBAO Journal of Instrumental Analysis Vol. 31 No. 2 127 ~ 132 - * 730070 PB - Eu - 3 8 min 0. 025 ~ 10 0. 025 ~ 25 0. 05 ~ 10 mg /L 3 1. 00 mg /L 3 6 RSD 4. 5% 0. 95% 3 101%~

Διαβάστε περισσότερα

Rapid Determination of Seven Fungicides in Citrus Fruits

Rapid Determination of Seven Fungicides in Citrus Fruits 228 Vol. 56, No. 5 7 27 5 29 * Rapid Determination of Seven Fungicides in Citrus Fruits Naoki Yoshioka*, Sachiko Hayashi and Tadaaki Inada Hyogo Prefectural Institute of Public Health and Consumer Sciences:

Διαβάστε περισσότερα

Electrolyzed-Reduced Water as Artificial Hot Spring Water

Electrolyzed-Reduced Water as Artificial Hot Spring Water /-,**- + +/ 0 +, - + + +, - + +. ++,3 +/. +. Electrolyzed-Reduced Water as Artificial Hot Spring Water Shoichi OKOUCHI +, Daisuke TAKEZAKI +, Hideyuki OHNAMI +, Yuhkoh AGISHI,, Yasuo KANROJI -, and Shigeo

Διαβάστε περισσότερα

and Selective Allylic Reduction of Allylic Alcohols and Their Derivatives with Benzyl Alcohol

and Selective Allylic Reduction of Allylic Alcohols and Their Derivatives with Benzyl Alcohol FeCl 3 6H 2 O-Catalyzed Disproportionation of Allylic Alcohols and Selective Allylic Reduction of Allylic Alcohols and Their Derivatives with Benzyl Alcohol Jialiang Wang, Wen Huang, Zhengxing Zhang, Xu

Διαβάστε περισσότερα

Protease-catalysed Direct Asymmetric Mannich Reaction in Organic Solvent

Protease-catalysed Direct Asymmetric Mannich Reaction in Organic Solvent Supplementary information for the paper Protease-catalysed Direct Asymmetric Mannich Reaction in Organic Solvent Yang Xue, Ling-Po Li, Yan-Hong He * & Zhi Guan * School of Chemistry and Chemical Engineering,

Διαβάστε περισσότερα

Studies on the Binding Mechanism of Several Antibiotics and Human Serum Albumin

Studies on the Binding Mechanism of Several Antibiotics and Human Serum Albumin 2005 63 Vol. 63, 2005 23, 2169 2173 ACTA CHIMICA SINICA No. 23, 2169 2173 a,b a a a *,a ( a 130012) ( b 133002), 26 K A 1.98 10 4, 1.01 10 3, 1.38 10 3, 5.97 10 4 7.15 10 4 L mol 1, n 1.16, 0.86, 1.19,

Διαβάστε περισσότερα

. K HPLC. 2 ~ 50 μg / ml r ~ mg / kg 15

. K HPLC. 2 ~ 50 μg / ml r ~ mg / kg 15 HPLC 527 2 ECONOMOU A FIELDEN P R. Applications potentialities and limitations of adsorptive stripping analysis on mercury film electrodesj. Trends Anal Chem 1997 16 5 286-292. 3 OL IVEIRA M FSACZK A AOKUMURA

Διαβάστε περισσότερα

Hydroxyapatite chromatography of guanidine denatured proteins 1. Guanidine containing phosphate buffer system

Hydroxyapatite chromatography of guanidine denatured proteins 1. Guanidine containing phosphate buffer system Original Hydroxyapatite chromatography of guanidine denatured proteins 1. Guanidine containing phosphate buffer system Tomohiko Yoshitake* 1),Shintaro Kobayashi 1),Tetsuro Ogawa 1) and Tsuneo Okuyama 2)

Διαβάστε περισσότερα

SFO-DLLME 12 DLLME DLLME SFO-DLLME. 1 L NaCl 1 mol /L H 3 PO 4

SFO-DLLME 12 DLLME DLLME SFO-DLLME. 1 L NaCl 1 mol /L H 3 PO 4 38 2010 10 FENXI UAXUE Chinese Journal of Analytical Chemistry 10 1400 ~ 1404 DOI 10. 3724 /P. J. 1096. 2010. 01400 - - * 442700 442000 - FO-DLLME - 3 24 1-200 μl 300 μl 1. 2 g NaCl 1 mol /L 3 PO 4 200

Διαβάστε περισσότερα

Supporting Information

Supporting Information Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Synthesis of 3-omosubstituted Pyrroles via Palladium- Catalyzed Intermolecular Oxidative Cyclization

Διαβάστε περισσότερα

Supporting Information

Supporting Information Supporting Information Lewis acid catalyzed ring-opening reactions of methylenecyclopropanes with diphenylphosphine oxide in the presence of sulfur or selenium Min Shi,* Min Jiang and Le-Ping Liu State

Διαβάστε περισσότερα

College of Life Science, Dalian Nationalities University, Dalian , PR China.

College of Life Science, Dalian Nationalities University, Dalian , PR China. Electronic Supplementary Material (ESI) for New Journal of Chemistry. This journal is The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2018 Postsynthetic modification

Διαβάστε περισσότερα

HPLC- ESI-MS HPLC-ESI-MS HPLC-ESI-MS HPLC 11 HPLC HPLC-ESI-MS. Asterias rollestoni Bell. LC- MS. Vol.11 No.1

HPLC- ESI-MS HPLC-ESI-MS HPLC-ESI-MS HPLC 11 HPLC HPLC-ESI-MS. Asterias rollestoni Bell. LC- MS. Vol.11 No.1 HPLC- ESI-MS * 200 Vol.11 No.1 ** 266061 266061 361005 266003 - HPLC-ESI-MS HPLC-ESI-MS HPLC HPLC-ESI-MS 11 HPLC - Asterias rollestoni Bell. [1~7] [1] [5~7] - - [8~] LC- MS * ** 173 2008-11-04 200-01-06

Διαβάστε περισσότερα

1 h, , CaCl 2. pelamis) 58.1%, (Headspace solid -phase microextraction and gas chromatography -mass spectrometry,hs -SPME - Vol. 15 No.

1 h, , CaCl 2. pelamis) 58.1%, (Headspace solid -phase microextraction and gas chromatography -mass spectrometry,hs -SPME - Vol. 15 No. 2 0 1 5 7 Journal of Chinese Institute of Food Science and Technology Vol. 15 No. 7 Jul. 2 0 1 5 1,2 1 1 1 1* ( 1 315211 2 315502) : ( ) : (E-Nose), - : GC-MS, 20.98% 2.5%, 53.15% 11.2%, 0. 51% 71.86%

Διαβάστε περισσότερα

Electronic Supplementary Information

Electronic Supplementary Information Electronic Supplementary Information NbCl 3 -catalyzed [2+2+2] intermolecular cycloaddition of alkynes and alkenes to 1,3-cyclohexadiene derivatives Yasushi Obora,* Keisuke Takeshita and Yasutaka Ishii*

Διαβάστε περισσότερα

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Sensitivity of [Ru(phen) 2 dppz] 2+ Light Switch Emission to Ionic Strength, Temperature, and DNA Sequence and Conformation Andrew W. McKinley, Per Lincoln and Eimer M. Tuite* SUPPLEMENTARY INFORMATION

Διαβάστε περισσότερα

J. of Math. (PRC) 6 n (nt ) + n V = 0, (1.1) n t + div. div(n T ) = n τ (T L(x) T ), (1.2) n)xx (nt ) x + nv x = J 0, (1.4) n. 6 n

J. of Math. (PRC) 6 n (nt ) + n V = 0, (1.1) n t + div. div(n T ) = n τ (T L(x) T ), (1.2) n)xx (nt ) x + nv x = J 0, (1.4) n. 6 n Vol. 35 ( 215 ) No. 5 J. of Math. (PRC) a, b, a ( a. ; b., 4515) :., [3]. : ; ; MR(21) : 35Q4 : O175. : A : 255-7797(215)5-15-7 1 [1] : [ ( ) ] ε 2 n n t + div 6 n (nt ) + n V =, (1.1) n div(n T ) = n

Διαβάστε περισσότερα

Study of Ne w Chemiluminescence Technique Recognition of Sodium Azide by External Reference Method

Study of Ne w Chemiluminescence Technique Recognition of Sodium Azide by External Reference Method 2004 62 8, 794 798 ACTA CHIMICA SINICA Vol 62, 2004 No 8, 794 798 Ξ Ξ ( 200032),, : :H 2 O 2 2CH 3 CN2 ( ) H 2 O 2 2CH 3 CN2N2 252 ( ),,, IgG Study of Ne w Chemiluminescence Technique Recognition of Sodium

Διαβάστε περισσότερα

Distribution of Mercury and Selenium in the Proteins of Porcine Liver and Kidney Under Different Mercury Exposure Level

Distribution of Mercury and Selenium in the Proteins of Porcine Liver and Kidney Under Different Mercury Exposure Level ISSN 100727626 CN 1123870ΠQ 2003 6 Chinese Journal of Biochemistry and Molecular Biology 19 (3) :377 382, 3,, (, 918 100039), (, 550002) (Sephadex G2100),, ;, ;, 11 kd (MT),,,,,,,,, Q51,X132 Distribution

Διαβάστε περισσότερα

Site-Selective Suzuki-Miyaura Cross-Coupling Reactions of 2,3,4,5-Tetrabromofuran

Site-Selective Suzuki-Miyaura Cross-Coupling Reactions of 2,3,4,5-Tetrabromofuran 1 Site-Selective Suzuki-Miyaura Cross-Coupling Reactions of 2,3,4,5-Tetrabromofuran Munawar Hussain, a Rasheed Ahmad Khera, a Nguyen Thai Hung, a Peter Langer* a,b a Institut für Chemie, Universität Rostock,

Διαβάστε περισσότερα

B

B 534 CHINESE JOURNAL OF FOOD HYGIENE 2011 23 6 100021 25% 2-5% 60 mmol / L 0. 1% 2- Supelco C 18 150 mm 4. 6 mm 5 μm KBH 4 0. 7 μg / L 4. 6% O657. 7 B 1004-8456 2011 06-0534-06 Determination of methylmercury

Διαβάστε περισσότερα

In vitro και in vivo φαρμακοκινητική ανάλυση των παραγώγων ανθρακινόνης σε φυτικά σκευάσματα

In vitro και in vivo φαρμακοκινητική ανάλυση των παραγώγων ανθρακινόνης σε φυτικά σκευάσματα ΑΡΙΣΤΟΤΕΛΕΙΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΘΕΣΣΑΛΟΝΙΚΗΣ ΤΜΗΜΑ ΦΑΡΜΑΚΕΥΤΙΚΗΣ ΤΟΜΕΑΣ ΦΑΡΜΑΚΟΓΝΩΣΙΑΣ ΦΑΡΜΑΚΟΛΟΓΙΑΣ ΠΡΟΓΡΑΜΜΑ ΜΕΤΑΠΤΥΧΙΑΚΩΝ ΣΠΟΥΔΩΝ ΚΑΤΕΥΘΥΝΣΗ ΦΑΡΜΑΚΟΛΟΓΙΑ ΚΑΙ ΘΕΡΑΠΕΥΤΙΚΗ In vitro και in vivo φαρμακοκινητική

Διαβάστε περισσότερα

Supporting Information

Supporting Information Supporting Information Wiley-VC 007 9 Weinheim, Germany ew ear Infrared Dyes and Fluorophores Based on Diketopyrrolopyrroles Dipl.-Chem. Georg M. Fischer, Dipl.-Chem. Andreas P. Ehlers, Prof. Dr. Andreas

Διαβάστε περισσότερα

) ; GSP ) ;PXD g, 100 ml

) ; GSP ) ;PXD g, 100 ml 30 (FENXI HUAXUE) 10 2002 10 Chinese Journal of Analytical Chemistry 1163 1167 3 3 3 (, 225002) PVC, 1. 0 10-4 1. 0 10-8 molπl, 58. 6 mvπdecade ; 2. 5 10-9 molπl, 2 3,,,,, 1, PVC Pretsch 1, EDTA,, 5 10-12

Διαβάστε περισσότερα

Rapid determination of soluble reactive silicate in seawater by flow injection analysis with spectrophotometric detection and its application

Rapid determination of soluble reactive silicate in seawater by flow injection analysis with spectrophotometric detection and its application 31 5 2011 5 Acta Scientiae Circumstantiae Vol 31 No 5 May 2011 2011 - J 31 5 935-940 Huang Y M Yuan D X Peng Y Z et al 2011 Rapid determination of soluble reactive silicate in seawater by flow injection

Διαβάστε περισσότερα

Extract Isolation Purification and Identification of Polysaccharides from Exocarp of Unripe Fruits of Juglans mandshurica

Extract Isolation Purification and Identification of Polysaccharides from Exocarp of Unripe Fruits of Juglans mandshurica 35 1 2 0 1 7 1 DOI 10. 13193 /j. issn. 1673-7717. 2017. 01. 035 1 1 1 1 2 1. 150036 2. 150040 D101 DEAE Cellulose 52 Sephacryl S - 300 - - PJP - 1a PJP - 3a 1. 34 10 3 Da 1. 70 10 7 Da - PJP - 3a 4. 56

Διαβάστε περισσότερα

Emulsifying Properties of Egg Yolk as a Function of Diacylglycerol Oil

Emulsifying Properties of Egg Yolk as a Function of Diacylglycerol Oil 354 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No.0, -/.-0* (,**0) 38 * * Emulsifying Properties of Egg Yolk as a Function of Diacylglycerol Oil Tomoko Kawakami, Kyoko Ohashi* and Atsuko Shimada Graduate

Διαβάστε περισσότερα

Supplementary Materials: Development of Amyloseand β-cyclodextrin-based Chiral Fluorescent Sensors Bearing Terthienyl Pendants

Supplementary Materials: Development of Amyloseand β-cyclodextrin-based Chiral Fluorescent Sensors Bearing Terthienyl Pendants S1 of S12 Supplementary Materials: Development of Amyloseand β-cyclodextrin-based Chiral Fluorescent Sensors Bearing Terthienyl Pendants Tomoyuki Ikai, Changsik Yun, Yutaka Kojima, Daisuke Suzuki, Katsuhiro

Διαβάστε περισσότερα

(bpy) 2 + , ECL, (polarization fluoroimmunometry) [8 ],HPLC [9 ] (CZE) [10 ] 2. 1 Ru(bpy) 3 Cl 2 6H 2 O Aldrich, ;

(bpy) 2 + , ECL, (polarization fluoroimmunometry) [8 ],HPLC [9 ] (CZE) [10 ] 2. 1 Ru(bpy) 3 Cl 2 6H 2 O Aldrich, ; 30 HUAXUE) 5 (FENXI 2002 5 Chinese Journal of Analytical Chemistry 513 517 3 (, 361005) (, 361005) [ Ru(bpy) 3 Cl 2 ] ( ECL), ECL, 0. 05 molπl (ph = 9. 0), (METH) ECL 6. 8 10-11 6. 8 10-3 molπl, 2 10-16

Διαβάστε περισσότερα

Supporting information for

Supporting information for Electronic Supplementary Material (ESI) for Green Chemistry. This journal is The Royal Society of Chemistry 2014 Supporting information for Mesoporous silica KIT-6 supported superparamagnetic CuFe 2 O

Διαβάστε περισσότερα

Hiyama Cross-Coupling of Chloro-, Fluoroand Methoxy- pyridyl trimethylsilanes : Room-temperature Novel Access to Functional Bi(het)aryl

Hiyama Cross-Coupling of Chloro-, Fluoroand Methoxy- pyridyl trimethylsilanes : Room-temperature Novel Access to Functional Bi(het)aryl Hiyama Cross-Coupling of Chloro-, Fluoroand Methoxy- pyridyl trimethylsilanes : Room-temperature Novel Access to Functional Bi(het)aryl Philippe Pierrat, Philippe Gros* and Yves Fort Synthèse Organométallique

Διαβάστε περισσότερα

Supporting information

Supporting information Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 2014 Supporting information Copper-catalysed intramolecular O-arylation: a simple

Διαβάστε περισσότερα

Supporting Information

Supporting Information Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Supporting Information A Convenient and Efficient Synthesis of Glycals by Zinc Nanoparticles

Διαβάστε περισσότερα

April 2013 Chinese Journal of Chromatography 380 ~ A

April 2013 Chinese Journal of Chromatography 380 ~ A 2013 4 Vol. 31 No. 4 April 2013 Chinese Journal of Chromatography 380 ~ 385 DOI 10. 3724 /SP. J. 1123. 2012. 10037-1 2 2 2 3* 1. 361005 2. 362006 3. 361005 - UHPLC-MS /MS BS EN 14362-1 2012 ISO 17234-1

Διαβάστε περισσότερα

A facile and general route to 3-((trifluoromethyl)thio)benzofurans and 3-((trifluoromethyl)thio)benzothiophenes

A facile and general route to 3-((trifluoromethyl)thio)benzofurans and 3-((trifluoromethyl)thio)benzothiophenes Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2014 A facile and general route to 3-((trifluoromethyl)thio)benzofurans and 3-((trifluoromethyl)thio)benzothiophenes

Διαβάστε περισσότερα

Rh(III)-Catalyzed C-H Amidation with N-hydroxycarbamates: A. new Entry to N-Carbamate Protected Arylamines

Rh(III)-Catalyzed C-H Amidation with N-hydroxycarbamates: A. new Entry to N-Carbamate Protected Arylamines Rh(III)-Catalyzed C-H Amidation with N-hydroxycarbamates: A new Entry to N-Carbamate Protected Arylamines Bing Zhou,* Juanjuan Du, Yaxi Yang,* Huijin Feng, Yuanchao Li Shanghai Institute of Materia Medica,

Διαβάστε περισσότερα

1-4 certified reference material CRM. DSC HPLC ± 0. 4 % k = 2 P = GBW

1-4 certified reference material CRM. DSC HPLC ± 0. 4 % k = 2 P = GBW 2014 6 33 6 779 * 1 1 2 3 1 2 1. 100050 2. 277500 3. 100050 DSC HPLC 99. 5 ± 0. 4 % k 2 P 0. 95 GBW09587 R978. 7 R927. 1 A 1004-0781 2014 06-0779 - 06 Purity Determination and Uncertainty Evaluation of

Διαβάστε περισσότερα

Supporting Information

Supporting Information Supporting Information 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Convenient and General Zinc-Catalyzed Borylation of Aryl Diazonium Salts and Aryltriazenes under Mild Conditions

Διαβάστε περισσότερα

Electronic Supplementary Information

Electronic Supplementary Information Electronic Supplementary Information Unprecedented Carbon-Carbon Bond Cleavage in Nucleophilic Aziridine Ring Opening Reaction, Efficient Ring Transformation of Aziridines to Imidazolidin-4-ones Jin-Yuan

Διαβάστε περισσότερα

Copper-Catalyzed Oxidative Dehydrogenative N-N Bond. Formation for the Synthesis of N,N -Diarylindazol-3-ones

Copper-Catalyzed Oxidative Dehydrogenative N-N Bond. Formation for the Synthesis of N,N -Diarylindazol-3-ones Electronic Supplementary Material (ESI) for Organic Chemistry Frontiers. This journal is the Partner Organisations 2016 Supporting information Copper-Catalyzed Oxidative Dehydrogenative - Bond Formation

Διαβάστε περισσότερα

N 2 O 15 N NH + NH 3 Rittenberg mg mmol L - 1 CuSO mg. 10 mol L. Pre-Concentration device PreCon

N 2 O 15 N NH + NH 3 Rittenberg mg mmol L - 1 CuSO mg. 10 mol L. Pre-Concentration device PreCon 50 1 Vol. 50 No. 1 2013 1 ACTA PEDOLOGICA SINICA Jan. 2013 N 2 O * N 210008 N 2 O N 2 O N N 2 O N N 5 ~ 20 μg N N 2 O N S8. 3 A N N 2 O N N N NO - 3 NO - 2 N N N MgO NH 3 Rittenberg 1 Dumas N 1 2 N N 2

Διαβάστε περισσότερα

Fluorinative Ring-opening of Cyclopropanes by Hypervalent Iodine Reagents. An Efficient Method for 1,3- Oxyfluorination and 1,3-Difluorination

Fluorinative Ring-opening of Cyclopropanes by Hypervalent Iodine Reagents. An Efficient Method for 1,3- Oxyfluorination and 1,3-Difluorination Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2016 Supporting Information Fluorinative Ring-opening of Cyclopropanes by Hypervalent Iodine

Διαβάστε περισσότερα

ENVIRONMENTAL CHEMISTRY

ENVIRONMENTAL CHEMISTRY 30 7 2011 7 ENVIRONMENTAL CHEMISTRY Vol 30No 7 July 2011 - N- * NDMA ** 100124 - GC-MS N- NDMA SPE SPE NDMA activated coconut charcoal SPE NDMA 1 μg L - 1 5 ng L - 1 15 μg L - 1 100 μg L - 1 NDMA 88 5%

Διαβάστε περισσότερα

Optimizing Microwave-assisted Extraction Process for Paprika Red Pigments Using Response Surface Methodology

Optimizing Microwave-assisted Extraction Process for Paprika Red Pigments Using Response Surface Methodology 2012 34 2 382-387 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com 212018 105 W 42 2 min 0. 631 TS202. 3 A 1000-2286 2012 02-0382 - 06 Optimizing

Διαβάστε περισσότερα

ΤΕΧΝΟΛΟΓΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΚΥΠΡΟΥ ΣΧΟΛΗ ΓΕΩΠΟΝΙΚΩΝ ΕΠΙΣΤΗΜΩΝ ΒΙΟΤΕΧΝΟΛΟΓΙΑΣ ΚΑΙ ΕΠΙΣΤΗΜΗΣ ΤΡΟΦΙΜΩΝ. Πτυχιακή εργασία

ΤΕΧΝΟΛΟΓΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΚΥΠΡΟΥ ΣΧΟΛΗ ΓΕΩΠΟΝΙΚΩΝ ΕΠΙΣΤΗΜΩΝ ΒΙΟΤΕΧΝΟΛΟΓΙΑΣ ΚΑΙ ΕΠΙΣΤΗΜΗΣ ΤΡΟΦΙΜΩΝ. Πτυχιακή εργασία ΤΕΧΝΟΛΟΓΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΚΥΠΡΟΥ ΣΧΟΛΗ ΓΕΩΠΟΝΙΚΩΝ ΕΠΙΣΤΗΜΩΝ ΒΙΟΤΕΧΝΟΛΟΓΙΑΣ ΚΑΙ ΕΠΙΣΤΗΜΗΣ ΤΡΟΦΙΜΩΝ Πτυχιακή εργασία ΜΕΛΕΤΗ ΠΟΛΥΦΑΙΝΟΛΩΝ ΚΑΙ ΑΝΤΙΟΞΕΙΔΩΤΙΚΗΣ ΙΚΑΝΟΤΗΤΑΣ ΣΟΚΟΛΑΤΑΣ Αναστασία Σιάντωνα Λεμεσός

Διαβάστε περισσότερα

Supplementary information

Supplementary information Electronic Supplementary Material (ESI) for MedChemComm. This journal is The Royal Society of Chemistry 2015 Supplementary information Synthesis of carboxyimidamide-substituted benzo[c][1,2,5]oxadiazoles

Διαβάστε περισσότερα

2015 10 Vol.33 No.10 October 2015 Chinese Journal of Chromatography 1040 ~ 1045 DOI 10.3724 / SP.J.1123.2015.06010 - / 1 1* 2 1 1 1 1 1 1. 210001 2. 210001 - / 0. 22 μm Carbohydrate 100 mm 2. 1 mm 2. 6

Διαβάστε περισσότερα

Fundamentals of Mass Spectrometry Fundamentals of Electrospray

Fundamentals of Mass Spectrometry Fundamentals of Electrospray J. Mass Spectrom. Soc. Jpn. Vol. 58, No. 4, 2010 REVIEW 8 Fundamentals of Mass Spectrometry Fundamentals of Electrospray Kenzo H>G6D@6 Kofu, YAMANASHI, JAPAN Clean Energy Research Center, University of

Διαβάστε περισσότερα

Supplementary Information

Supplementary Information Electronic upplementary Material (EI) for Photochemical & Photobiological ciences. This journal is The Royal ociety of Chemistry and wner ocieties 214 upplementary Information elective and sensitive fluorescence-shift

Διαβάστε περισσότερα

Novel and Selective Palladium-Catalyzed Annulation of 2-Alkynylphenols to Form 2-Substituted 3-Halobenzo[b]furans. Supporting Information

Novel and Selective Palladium-Catalyzed Annulation of 2-Alkynylphenols to Form 2-Substituted 3-Halobenzo[b]furans. Supporting Information Novel and Selective Palladium-Catalyzed Annulation of 2-Alkynylphenols to Form 2-Substituted 3-Halobenzo[b]furans Liang Yun, Shi Tang, Xu-Dong Zhang, Li-Qiu Mao, Ye-Xiang Xie and Jin-Heng Li* Key Laboratory

Διαβάστε περισσότερα

Investigation on Fast Determination of Trace N2Nitrosamines Assisted by Microwave Radiation

Investigation on Fast Determination of Trace N2Nitrosamines Assisted by Microwave Radiation 2002 60 5, 876 881 ACTA CHIMICA SINICA Vol 60, 2002 No 5, 876 881 Ξ ( 210093), NOBr NO x ( x < 2),, CrO 3, NO x ( x < 2) NO 2, 90 %, (TEA) (76 % 96 %) ; 2 10-8 mol/ L /,, 3 min, ( ),, Investigation on

Διαβάστε περισσότερα

Determination of Benzalkonium Chloride Homologs in Complex Chemical Disinfectant by High-Performance Liquid Chromatography

Determination of Benzalkonium Chloride Homologs in Complex Chemical Disinfectant by High-Performance Liquid Chromatography 2011 12 December 2011 ROCK A MINERAL ANALYSIS Vol. 30 No. 6 677 ~ 682 0254-5357 2011 06-0677 - 06 * 210093 Platisil ODS 5 μm 250 mm 4. 6 mm - 0. 2 mol /L 1% V /V ph = 6. 0 85 15 220 nm 3 0. 002 ~ 5 mg

Διαβάστε περισσότερα

HS - SPME - GC - MS. Abstract: Objective A rapid method was developed for multi2residues determination of organochlorine and pyrethroids

HS - SPME - GC - MS. Abstract: Objective A rapid method was developed for multi2residues determination of organochlorine and pyrethroids HS SPME GC MS 289 HS SPME GC MS 1,2 2 3 2,3 2 1 2 (11, 110161 ; 21, 100050 ; 31, 100069) :, 31 (23, 8 ), 6 10, 01002012gΠL,85 %110 %,3 %12 % : ;;,; ;,; Headspace Solid2Phase Microextraction Combined with

Διαβάστε περισσότερα

Determination of Organophosphate Pesticides in Soil Samples by Accelerated Solvent Extraction-Gas Chromatography

Determination of Organophosphate Pesticides in Soil Samples by Accelerated Solvent Extraction-Gas Chromatography 2010 10 October 2010 ROCK AND MINERAL ANALYSIS Vol. 29 No. 5 503 ~ 507 0254 5357 2010 05 0503 05-1 2 3 1 1 1. 100037 2. 710021 3. 100083 13 Carb Rtx - OPP2-0. 67 ~ 1. 50 ng /ml 0. 67 ~ 600 ng /ml 0. 999

Διαβάστε περισσότερα

Shiji ~ DOI /j. cnki. hxsj ARL Perform'X. Pb60Sn40 GBW GBW GBW % ~ 4.

Shiji ~ DOI /j. cnki. hxsj ARL Perform'X. Pb60Sn40 GBW GBW GBW % ~ 4. X- 43 DOI 10. 13822 /j. cnki. hxsj. 2016. 01. 010 2016 38 1 43 ~ 46 51 X- * 214431 X- 6 0. 999 0. 02% ~ 4. 2% X- O657. 34 A 0258-3283 2016 01-0043-04 Determination of As Cu Sb Sn Bi and Pb in Lead Alloy

Διαβάστε περισσότερα

Supporting Information

Supporting Information Supporting Information Copper/Silver Cocatalyzed Oxidative Coupling of Vinylarenes with ICH 2 CF 3 or ICH 2 CHF 2 Leading to β-cf 3 /CHF 2 -Substituted Ketones Niannian Yi, Hao Zhang, Chonghui Xu, Wei

Διαβάστε περισσότερα

74.3 % ~ 89.1 % 1.08 h

74.3 % ~ 89.1 % 1.08 h 31 Traditional Chinese Drug Research & Clinical Pharmacology 11 May Vol.22 No.3 /μg ml -1 2 - Figure 2 Concentration-time curve of the puerarin in compound puerarin or puerarin injection 2 4 6 6 413-414.

Διαβάστε περισσότερα

Comparison of HPLC fingerprint between enzymatic Calculus bovis and natural Calculus bovis

Comparison of HPLC fingerprint between enzymatic Calculus bovis and natural Calculus bovis HPLC * 271016 HPLC - DAD DiamonsilC 18 250 mm 4. 6 mm 5 μm - -1% 370 nm 1. 0 ml /min 40 R282. 7 A 1001-1528 2011 01-0001-05 Comparison of HPLC fingerprint between enzymatic Calculus bovis and natural Calculus

Διαβάστε περισσότερα

Diversity-oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens

Diversity-oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens Supporting Information for Diversity-oriented Enzymatic Modular Assembly of ABO Histo-blood Group Antigens Jinfeng Ye,, Xian-wei Liu,, Peng Peng, Wen Yi, Xi Chen, Fengshan Wang,, Hongzhi Cao*,, National

Διαβάστε περισσότερα

Synthesis of α-deoxymono and -Difluorohexopyranosyl. 1-Phosphates and Kinetic Evaluation with Thymidylyl- and. Guanidylyltransferases

Synthesis of α-deoxymono and -Difluorohexopyranosyl. 1-Phosphates and Kinetic Evaluation with Thymidylyl- and. Guanidylyltransferases Synthesis of α-deoxymono and -Difluorohexopyranosyl 1-Phosphates and Kinetic Evaluation with Thymidylyl- and Guanidylyltransferases Jian-She Zhu, a Nicole E. McCormick, a Shannon C. Timmons, b,c David

Διαβάστε περισσότερα

Supporting Information

Supporting Information Supporting Information Rhodium-catalyzed Intramolecular Dehydrogenative Aryl Aryl Coupling Using Air as Terminal Oxidant Hannah Baars, 1,2 Yuto Unoh, 1 Takeshi Okada, 1 Koji Hirano, 1 Tetsuya Satoh,* 1,3

Διαβάστε περισσότερα

9-amino-(9-deoxy)cinchona alkaloids-derived novel chiral phase-transfer catalysts

9-amino-(9-deoxy)cinchona alkaloids-derived novel chiral phase-transfer catalysts Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 2014 9-amino-(9-deoxy)cinchona alkaloids-derived novel chiral phase-transfer

Διαβάστε περισσότερα

Supplementary Information

Supplementary Information Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2017 Supplementary Information Immobilization of Pseudomonas fluorescens lipase on silk fibroin

Διαβάστε περισσότερα

Investigation of ORP (Oxidation-Reduction Potential) Measurement on Sulfur Springs and Its Application on Hot Spring Waters in Nozawa Onsen

Investigation of ORP (Oxidation-Reduction Potential) Measurement on Sulfur Springs and Its Application on Hot Spring Waters in Nozawa Onsen J. Hot Spring Sci. 0* 123* *+* + + - -+. 3 Investigation of ORP (Oxidation-Reduction Potential) Measurement on Sulfur Springs and Its Application on Hot Spring Waters in Nozawa Onsen Takuya M Hidekazu

Διαβάστε περισσότερα

k A = [k, k]( )[a 1, a 2 ] = [ka 1,ka 2 ] 4For the division of two intervals of confidence in R +

k A = [k, k]( )[a 1, a 2 ] = [ka 1,ka 2 ] 4For the division of two intervals of confidence in R + Chapter 3. Fuzzy Arithmetic 3- Fuzzy arithmetic: ~Addition(+) and subtraction (-): Let A = [a and B = [b, b in R If x [a and y [b, b than x+y [a +b +b Symbolically,we write A(+)B = [a (+)[b, b = [a +b

Διαβάστε περισσότερα

Design and Solid Phase Synthesis of New DOTA Conjugated (+)-Biotin Dimers Planned to Develop Molecular Weight-Tuned Avidin Oligomers

Design and Solid Phase Synthesis of New DOTA Conjugated (+)-Biotin Dimers Planned to Develop Molecular Weight-Tuned Avidin Oligomers Electronic Supplementary Material (ESI) for rganic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 2015 Supplementary Information Design and Solid Phase Synthesis of New DTA Conjugated

Διαβάστε περισσότερα

GS3. A liner offset equation of the volumetric water content that capacitance type GS3 soil moisture sensor measured

GS3. A liner offset equation of the volumetric water content that capacitance type GS3 soil moisture sensor measured J. Jpn. Soc. Soil Phys. No. 130, p.19 25 (2015) GS3 1 2 A liner offset equation of the volumetric water content that capacitance type GS3 soil moisture sensor measured Shoichi MITSUISHI 1 and Masaru MIZOGUCHI

Διαβάστε περισσότερα

Rapid Raman spectra identification and determination of levofloxacin hydrochloride injection *

Rapid Raman spectra identification and determination of levofloxacin hydrochloride injection * DOI:0.655/j.0254-793.20.09.039 75 * 2 2 3. 200435 2. 20203 3. 20000 ph R97 A 0254-793 20 09-75 - 05 Rapid Raman spectra identification and determination of levofloxacin hydrochloride injection * CHEN Bin

Διαβάστε περισσότερα

Supporting Information One-Pot Approach to Chiral Chromenes via Enantioselective Organocatalytic Domino Oxa-Michael-Aldol Reaction

Supporting Information One-Pot Approach to Chiral Chromenes via Enantioselective Organocatalytic Domino Oxa-Michael-Aldol Reaction Supporting Information ne-pot Approach to Chiral Chromenes via Enantioselective rganocatalytic Domino xa-michael-aldol Reaction Hao Li, Jian Wang, Timiyin E-Nunu, Liansuo Zu, Wei Jiang, Shaohua Wei, *

Διαβάστε περισσότερα

: Monte Carlo EM 313, Louis (1982) EM, EM Newton-Raphson, /. EM, 2 Monte Carlo EM Newton-Raphson, Monte Carlo EM, Monte Carlo EM, /. 3, Monte Carlo EM

: Monte Carlo EM 313, Louis (1982) EM, EM Newton-Raphson, /. EM, 2 Monte Carlo EM Newton-Raphson, Monte Carlo EM, Monte Carlo EM, /. 3, Monte Carlo EM 2008 6 Chinese Journal of Applied Probability and Statistics Vol.24 No.3 Jun. 2008 Monte Carlo EM 1,2 ( 1,, 200241; 2,, 310018) EM, E,,. Monte Carlo EM, EM E Monte Carlo,. EM, Monte Carlo EM,,,,. Newton-Raphson.

Διαβάστε περισσότερα

Eur. J. Inorg. Chem WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 2007 ISSN SUPPORTING INFORMATION

Eur. J. Inorg. Chem WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 2007 ISSN SUPPORTING INFORMATION Eur. J. Inorg. Chem. 2007 WILEY-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, 2007 ISSN 1434 1948 SUPPORTING INFORMATION Title: Synthesis of Cyclic Carbonates from Atmospheric Pressure Carbon Dioxide Using

Διαβάστε περισσότερα

ΜΕΛΕΤΗ ΤΩΝ ΣΥΝΘΗΚΩΝ ΕΚΧΥΛΙΣΗΣ ΦΑΙΝΟΛΙΚΩΝ ΣΥΣΤΑΤΙΚΩΝ ΑΠΟ ΤΟ ΦΥΤΟ Sideritis raeseri ΜΕ ΤΗ ΧΡΗΣΗ ΜΙΚΡΟΚΥΜΑΤΩΝ

ΜΕΛΕΤΗ ΤΩΝ ΣΥΝΘΗΚΩΝ ΕΚΧΥΛΙΣΗΣ ΦΑΙΝΟΛΙΚΩΝ ΣΥΣΤΑΤΙΚΩΝ ΑΠΟ ΤΟ ΦΥΤΟ Sideritis raeseri ΜΕ ΤΗ ΧΡΗΣΗ ΜΙΚΡΟΚΥΜΑΤΩΝ ΜΕΛΕΤΗ ΤΩΝ ΣΥΝΘΗΚΩΝ ΕΚΧΥΛΙΣΗΣ ΦΑΙΝΟΛΙΚΩΝ ΣΥΣΤΑΤΙΚΩΝ ΑΠΟ ΤΟ ΦΥΤΟ Sideritis raeseri ΜΕ ΤΗ ΧΡΗΣΗ ΜΙΚΡΟΚΥΜΑΤΩΝ Ι. Σαρακατσιάνος 1,2, Κ. Αδαμόπουλος 1, Β. Σαμανίδου 3, Α. Γούλα 4 1. Εργαστήριο Τεχνολογίας Βιομηχανιών

Διαβάστε περισσότερα

Studies on Diazetetraene Cobalt( ) Metallic Complex as Neutral Carriers for the Preparation of Salicylate2Selective Ion Electrodes

Studies on Diazetetraene Cobalt( ) Metallic Complex as Neutral Carriers for the Preparation of Salicylate2Selective Ion Electrodes 2002 60 12, 2192 2196 ACTA CHIMICA SINICA Vol 60, 2002 No 12, 2192 2196 ( 400715) Ξ Ξ ( ) {3,3 2[1,22 ( ) ] 22,42 ( ) } [ Co ( )2EBIBP] (Sal - ), Hofmeister, Sal - µ I - > SCN - > NO 2 - > SO 2 4 - > NO

Διαβάστε περισσότερα

Copper-promoted hydration and annulation of 2-fluorophenylacetylene derivatives: from alkynes to benzo[b]furans and benzo[b]thiophenes

Copper-promoted hydration and annulation of 2-fluorophenylacetylene derivatives: from alkynes to benzo[b]furans and benzo[b]thiophenes Supporting Information for Copper-promoted hydration and annulation of 2-fluorophenylacetylene derivatives: from alkynes to benzo[b]furans and benzo[b]thiophenes Yibiao Li* 1, Liang Cheng 1, Xiaohang Liu

Διαβάστε περισσότερα

Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea

Natural Products Research Institute, College of Pharmacy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea Supporting Information Nicrophorusamides A and B, Antibacterial Chlorinated Cyclic Peptides from a Gut Bacterium of the Carrion Beetle Nicrophorus concolor Yern-Hyerk Shin, Suhyun Bae, Jaehoon Sim,,ǁ Joonseong

Διαβάστε περισσότερα

Effect of extract method on pharmacokinetics of baicalin and berberine in dogs

Effect of extract method on pharmacokinetics of baicalin and berberine in dogs 7. J. 54-66. 2009 305 351-353. 12Yu LBridgers APolli Jet al. Vitamin E-TPGS increases ab- 8. sorption flux of an HIV protease inhibitor by enhancing its solubility and permeability J. J Pharm J. 2007234

Διαβάστε περισσότερα

Electronic Supplementary Information (ESI)

Electronic Supplementary Information (ESI) Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2016 Electronic Supplementary Information (ESI) Cyclopentadienyl iron dicarbonyl (CpFe(CO) 2 ) derivatives

Διαβάστε περισσότερα

Physical and Chemical Properties of the Nest-site Beach of the Horseshoe Crab Rehabilitated by Sand Placement

Physical and Chemical Properties of the Nest-site Beach of the Horseshoe Crab Rehabilitated by Sand Placement J. Jpn. Soc. Soil Phys. No. 33, p.//0-,**/ ******* * Physical and Chemical Properties of the Nest-site Beach of the Horseshoe Crab Rehabilitated by Sand Placement Masami OHTSUBO*, Hidekazu ISHIDA**, Hisakatsu

Διαβάστε περισσότερα

Studies on the Interaction between Copper( ) Complex with Phenanthroline and L2Methionine Ligands and D NA

Studies on the Interaction between Copper( ) Complex with Phenanthroline and L2Methionine Ligands and D NA 2003 61 2, 245 250 ACTA CHIMICA SINICA Vol 61, 2003 No 2, 245 250 ( ) DNA a, b c b a Ξ, a b b b Ξ ( a 510275) ( b 510631) ( c 510642) ph = 6186, ( ) ( EB) [ Cu (phen) ( H 2 O) ( L2Met) ] + (phen = 1,102,

Διαβάστε περισσότερα

Study on Purification Technology and Antioxidant Activity of Total Flavonoid from Eriobotryae Folium

Study on Purification Technology and Antioxidant Activity of Total Flavonoid from Eriobotryae Folium Interleukin-1beta causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung [J]. Am J Respir Cell Mol Biol, 2005, 32(4): 311-318. [10] FAN C H, LI S Y, LI M, et al. The clinical

Διαβάστε περισσότερα

Supporting Information

Supporting Information Electronic upplementary Material (EI) for Green Chemistry. This journal is The Royal ociety of Chemistry 204 upporting Information ynthesis of sulfonamides via I 2 -mediated reaction of sodium sulfinates

Διαβάστε περισσότερα

Reaction of a Platinum Electrode for the Measurement of Redox Potential of Paddy Soil

Reaction of a Platinum Electrode for the Measurement of Redox Potential of Paddy Soil J. Jpn. Soc. Soil Phys. No. +*0, p.- +*,**1 Eh * ** Reaction of a Platinum Electrode for the Measurement of Redox Potential of Paddy Soil Daisuke MURAKAMI* and Tatsuaki KASUBUCHI** * The United Graduate

Διαβάστε περισσότερα

Supplementary Table 1. Construct List with key Biophysical Properties of the expression

Supplementary Table 1. Construct List with key Biophysical Properties of the expression SPINE Benchmark Target ID Well Code Tag (N or C) Fusion MW (kda) Fusion pi Cleavage site Prot MW (Da) Prot pi 1 A1 OPPF 2585 N-His6 21.75 6.41 Protease 3C 19.77 5.43 2 B1 OPPF 2586 N-His6 15.6 6.27 Protease

Διαβάστε περισσότερα

J. of Math. (PRC) Banach, , X = N(T ) R(T + ), Y = R(T ) N(T + ). Vol. 37 ( 2017 ) No. 5

J. of Math. (PRC) Banach, , X = N(T ) R(T + ), Y = R(T ) N(T + ). Vol. 37 ( 2017 ) No. 5 Vol. 37 ( 2017 ) No. 5 J. of Math. (PRC) 1,2, 1, 1 (1., 225002) (2., 225009) :. I +AT +, T + = T + (I +AT + ) 1, T +. Banach Hilbert Moore-Penrose.. : ; ; Moore-Penrose ; ; MR(2010) : 47L05; 46A32 : O177.2

Διαβάστε περισσότερα

Enantioselective Organocatalytic Michael Addition of Isorhodanines. to α, β-unsaturated Aldehydes

Enantioselective Organocatalytic Michael Addition of Isorhodanines. to α, β-unsaturated Aldehydes Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 2016 Enantioselective Organocatalytic Michael Addition of Isorhodanines to α,

Διαβάστε περισσότερα

HPLC-FL. polycyclic aromatic hydrocarbon; PAH PAH PAH HPLC PAH HPLC US EPA DNA 4 US EPA. 2 1 US EPA 610 PAH Mix PAH PAH

HPLC-FL. polycyclic aromatic hydrocarbon; PAH PAH PAH HPLC PAH HPLC US EPA DNA 4 US EPA. 2 1 US EPA 610 PAH Mix PAH PAH BUNSEKI KAGAKU Vol. 63, No. 1, pp. 23-292014 2014 The Japan Society for Analytical Chemistry 23 HPLC 1 1 1 Thanyarat CHUESAARD 2 1 1 1 polycyclic aromatic hydrocarbon; PAH 1 PAH / IARC PAH 2 10 46 PAH

Διαβάστε περισσότερα

+, - functional food, nutraceutical , Food for Specified Health Uses (FoSHU) in future Makoto SHIMIZU, ,*+*

+, - functional food, nutraceutical , Food for Specified Health Uses (FoSHU) in future Makoto SHIMIZU, ,*+* -*,* +32. Food for Specified Health Uses (FoSHU) in future Makoto SHIMIZU, +, - - - 1 (+ -) functional food, nutraceutical +33+ +33-,,*+* / 3**,**1 1,***,**3 in vitro + + + (.), + MBP g A +, A Ca Ca a

Διαβάστε περισσότερα

Supporting Information

Supporting Information Supporting Information Montmorillonite KSF-Catalyzed One-pot, Three-component, Aza-Diels- Alder Reactions of Methylenecyclopropanes With Arylaldehydes and Aromatic Amines Li-Xiong Shao and Min Shi* General

Διαβάστε περισσότερα

(Glycoside Hydrolase, GH) (1) α-n (NagBb) (Hyp) β-l- (HypBA2) β-l- (HypBA1) HypBA2. SeMet-SAD. (2) Bifidobacterium bifidum, B. longum, B. B.

(Glycoside Hydrolase, GH) (1) α-n (NagBb) (Hyp) β-l- (HypBA2) β-l- (HypBA1) HypBA2. SeMet-SAD. (2) Bifidobacterium bifidum, B. longum, B. B. (Glycoside Hydrolase, GH) (1) B α- (AgaBb) α-n (NagBb) (Hyp) β-β-l- (HypBA2) β-l- (HypBA1) B (2) Bifidobacterium bifidum, B. longum, B. breve B. bifidum B. bifidum (3)β-L- β-l- EC 3.2.1.187 EC 3.2.1.185

Διαβάστε περισσότερα

Highly enantioselective cascade synthesis of spiropyrazolones. Supporting Information. NMR spectra and HPLC traces

Highly enantioselective cascade synthesis of spiropyrazolones. Supporting Information. NMR spectra and HPLC traces Highly enantioselective cascade synthesis of spiropyrazolones Alex Zea a, Andrea-Nekane R. Alba a, Andrea Mazzanti b, Albert Moyano a and Ramon Rios a,c * Supporting Information NMR spectra and HPLC traces

Διαβάστε περισσότερα

Divergent synthesis of various iminocyclitols from D-ribose

Divergent synthesis of various iminocyclitols from D-ribose Electronic Supplementary Material (ESI) for rganic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 205 Divergent synthesis of various iminocyclitols from D-ribose Ramu Petakamsetty,

Διαβάστε περισσότερα

Copper(II) complexes of salicylaldehydes and 2 hydroxyphenones: Synthesis, Structure,

Copper(II) complexes of salicylaldehydes and 2 hydroxyphenones: Synthesis, Structure, Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2015 Copper(II) complexes of salicylaldehydes and 2 hydroxyphenones: Synthesis, Structure, Thermal

Διαβάστε περισσότερα