Development of Methods for the Specific Enrichment of Glycopeptides for the LC/MS Analysis
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1 LC/MS Development of Methods for the Specific Enrichment of Glycopeptides for the LC/MS Analysis Masahiro Yodoshi, Tomoko Ikuta, Yukie Mohri, Takehiro Oyama, Shigeo Suzuki School of Pharmacy, Kinki University, 3 4 1, Kowakae, Higashi osaka, , Japan. Abstract Glycosylation affects various biological functions of proteins, and it also recognized as an indicator of disease. Recent glycoproteomics studies require methods capable to enrich glycopeptides from protease digests, because MS sensitivity of glycopeptides is much lower than that of peptides. In addition salts or detergents added in the course of sample preparation impair ionization efficiency of glycoconjugates. Therefore, many researchers have been surveyed the methods to enrich glycoproteins and glycopeptides from crude samples such as urine, plasma and cells. Here we overview recent progresses of the enrichment methods of glycopeptides and glycans applied for the determination of glycosylation sites, structural analysis of carbohydrates, and their applications to glycomics or glycoproteomics studies. After that we introduced our recent studies on three novel methods for the enrichment of glycoconjugates; serotonin immobilized silica SPE for trapping sialic acid containing glycoconjugates, borate form anion exchanger to trap all of glycoconjugates, and various lectins and centrifugal ultrafiltration membrane for specific recovery of glycoconjugates based on the specificities of lectins to glycan structures. Keywords: solid phase extraction, glycoproteomics, MS, enrichment, serotonin bonded silica, borate form anion exchanger, lectin. [1] [2] [3, 4] microheterogeneity [5 7] School of Pharmacy, Kinki University, 3 4 1, Kowakae, Higashi osaka, , Japan. Correspondence autor : Shigeo Suzuki Tel : Fax : E mail : suzuki@phar.kindai.ac.jp
2 [7 11] LC MALDI MS LC ESI MS LC /ESI MS [12 15] [16 21] LC MS MS n [22] LC/MS SPE Scheme 1 [23] Scheme 1. Compexation between boric acid or phenylboronic acid and saccharides. OH [24 27] [28] [29] [30] [31] SPE SPE Hypercarb TFA
3 MS [32 38] Packer SPE [39] [40] MALDI MS [41] SPE hydrophilic interaction chromatography; HILIC [42 44] HILIC [18, 45 47] HILIC LC/ESI MS [48 50] Takegawa Deguchi zwitterionic HILIC ZIC HILIC [51 54] HILIC Shimizu N N [55] SPE MALDI MS MS n [56, 57] v/v v/v Yu SPE MALDI MS [58] [47, 57] [59] [60] Con ALCA Con A LCA [61 63] [64] [65] MALDI MS LC/ESI MS [28] [66, 67] [68, 69] Hirabayashi [70 72] [73] [74] MAM
4 SSA 5HT N NeuAc Sturgeon [75] µm nm γ 5HT 5HT Scheme 2 [65] SPE Fig. 1 NeuAc M AP Fig. 2 Fig. 2. Reversed phase HPLC analysis of AP labeled glycans derived from human transferrin before (a) and after (b) specific extraction with Si 5HT SPE cartridge. Analytical conditions: column, ODS silica column ( mm, Nacalai Tesque); buffer, (A)10 mm NaH2PO4 (ph 3.8), (B)buffer A containing 0.5% 1 BuOH; eluent, 70% to 5% of buffer A for 100 min; flow rate, 1.0 ml/min; detection, fluorescence at 310 (ex)/390 (em) nm. Scheme 2. Synthesis of the serotonin immobilized silica. Fig. 1. Procedure for purification of sialylated glycan derivatives and/or peptides using Si 5HT SPE. Scheme 1 SPE ph B RNase B Fig. 3 LC/MS TIC Fig. 3(a) SPE
5 Fig. 3. Total ion current chromatograms of reversed phase LC/ESI MS of tryptic digests of ribonuclease B before (a) and after (b) specific extraction with borate form anion exchange resins. MS scan range: m/z. GlcNac; N acethyl glucosamine, Man; Mannose. Fig. 4. Total ion current chromatograms of reversed phase LC/ESI MS of tryptic digests of ribonuclease B before (a) and after (b) specific extraction with Con A. MS scan range: m/z. GlcNac; N acethyl glucosamine, Man; Mannose. Fig. 3(b) SPE µg MNaCl mm CaCl mm MnCl mm MgCl M Tris HCl ph µg TFA ml Fig. 4 RNase B Con A α SSA SPE SPE
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