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1 ,22 (1) :60 64 Journal of Nuclear Agricultural Sciences : (2008) , (11, ; 21, ) : H 2 O 2,, min NaOH 0116molΠL H 2 O %, %, : ; ; ; OPTIMIZATION OF AL KALINE EXTRACTION CONDITIONS FOR ARABINOXYLAN FROM WHEAT BRAN ZHANG Xiao2na 1 WANG Shi2ping 1 ZHOU Su2mei 2 FU Xiu2min 1 (11 College of food science and nutritional engineering, China Agricultural University, Beijing ; 21 Institute Food Science & Technology, Chinese Academy of Agricultural Sciences, Beijing ) Abstract : In order to enhance the extraction yield of arabinoxylan from wheat bran, the effect of temperature, time, the concentrations of sodium hydroxide and hydrogen peroxide and shaking speed on yield of arabinoxylan were investigated. Based on this, the optimization of extraction conditions of arabinoxylan was carried out and mathematical model was established through the quadratic regression orthogonal rotary combination design. Results indicated that when the extraction temperature, time, the concentrations of sodium hydroxide and hydrogen peroxide were 88, 200min, 0116molΠL and 115 % respectively, the yield ( Y) reached the maximum (21123 %), which was consistent with the validate experiment result. Key words :arabinoxylan ; alkaline extraction ; orthogonal rotation combination design ; optimization 2000 (Arabinoxylan,AX, ), 20 % [1 ],AX : ; ; [2 ] AX, [3 ], (015molΠL NaOH Ba (OH) 2 Ca (OH) 2 ) [4 7 ],,,, [8,9 ], AX ( Alkaline arabinoxylan,aeax) extractable,,,,,, : : : ( ) : (19772),,,, E2mail : (19712),,,,, Tel : ; E2mail yahoo. com. cn

2 1 61, [10 ] NaOH H 2 O 2 4,, AX, rΠmin,AEAX 2, 1, 115 % H 2 O 2, 140rΠmin,AEAX 100rΠmin,,H 2 O 2 140rΠmin %H 2 O 2 ( 1 20) NaOH H 2 O 2 (4200rΠmin 10min) ( ) (AEAX) AEAX ( ) : - [11 ] ;AEAX ( ) : AEAX Π ( %) ( rΠmin) ( Π gπml ) ( ) ( min) NaOH ( molπl) H 2 O 2 ( 30 % 0 % 015 % 110 % 115 % 310 %) 6 AEAX, AEAX, 11313, rΠmin, 4 (1Π2) NaOH H 2 O 2, 23 DPS AX AEAX 1, NaOH 0110molΠL, 1 20,60 1h,,AEAX 1 AEAX Fig. 1 Influence of shaking speed 2 H 2 O 2 AEAX Fig. 2 Influence of shaking speed and 115 % hydrogen peroxide AEAX,, ( 3) : NaOH 0110molΠL,100rΠmin 60 1h,,AEAX 1 20,AEAX,, AEAX 4, NaOH 0110molΠL, 1 20,100rΠ min 1h,AEAX,90,,,,,,

3 AEAX Fig. 3 Influence of the wheat branπwater ratio 6 NaoH AEAX Fig. 6 Influence of alkaline concentration 4 AEAX Fig. 4 Influence of the action temperature AEAX AEAX 5, NaOH 0110molΠL, 1 20,100rΠ min 60,AEAX, 2h min AEAX,,,,,,, 0110molΠL H 2 O 2 AEAX 7, NaOH 0110molΠL, 1 20,100rΠ min 60,H 2 O 2 AEAX 60min,015 % 110 % H 2 O 2 AEAX 115 % 310 %, 120min,, H 2 O 2,, H 2 O 2, H 2 O % 110 %, min 5 AEAX Fig. 5 Influence of the time NaOH AEAX 6, 1 20, 100rΠmin 60 1h, 7 H 2 O 2 AEAX Fig. 7 Influence of hydrogen peroxide concentration 212 AEAX [10 ],

4 1 63 2, (X 1 ) (X 2 ) NaOH (X 3 ) H 2 O 2 (X 4 ) 4, 5 ( 1) levels 1 Table 1 Factors and levels of the orthogonal (X 1 ) temperature ( ) regression tests (X 2 ) time (min) NaOH (X 3 ) concentration of sodium hydroxide (molπl) H 2 O 2 (X 4 ) concentration of hydrogen peroxide ( %) , DPS, : Y = X X X X X X X X X 1 X X 1 X X 1 X X 2 X X 2 X X 3 X 4 (1) , F, 3 F 0105 (2, 6) = 5114 < F 1 = < F 0101 (2, 6) = 10192, = 0105, = 0101,,, F 2 = > F 0101 (14, 8) = 5156, = 0101, , = 0110, : Y = X X X X X X 2 4 (2) AEAX 4 AEAX NaOH H 2 O 2 R 2 = Π %, %, 4124 % Table 2 The quadratic rotary combination design and treat ment experimentation results Experimental scheme X1 X2 X3 X4 AEAX yields of AEAX ( %) [10 ] 3, : Y 1 = X X 2 1 Y 2 = X X 2 2 Y 3 = X X 2 3 Y 4 = X X 2 4 ( ) 4 :NaOH(X 3 ) (X 1 ) (X 2 ) H 2 O 2 ( X 4 ) ( 8), 8, Xi 11682, NaOH H 2 O 2, 4 NaOH 1 AEAX, 1, H 2 O AEAX, 015,, AEAX

5 64 Table 3 source of variation 3 Experimentation results and variance analysis sum of degree of squares freedom mean F value of F p significant level X X X X X X X X X 1 X X 1 X X 1 X X 2 X X 2 X X 3 X Regression F 2 = Excess Lack of fit F 1 = Error Sum : , Note : 3 indicates significant difference at 5 %leve1, 33means significant at 1 % level. Π = 1102,,, : NaOH H 2 O min 0116molΠL 115 %, %, [12 ],, AX,, AX, AX H 2 O 2 0 (115 %), H 2 O % 110 %, 3 311, : 1 20, 140rΠmin, 80 90, 120min, NaOH 0110molΠL H 2 O % 312,, DPS : 88, 200min,NaOH 0116molΠL, H 2 O %, AEAX %, : NaOH H 2 O %, % Journal of Nuclear Agricultural Sciences 2008,22 (1) : min 0116molΠL : 8 Fig. 8 The main effects of experimental factors (1), NaOH H 2 O DPS, AEAX : (88 ), ( 200min), NaOH (0116molΠL), H 2 O 2 0 (115 %) AEAX %, AEAX %, [ 1 ] Maes C, Delcour J A. Structural characterization of water2extractable and water2unextractable arabinoxylans in wheat bran. Cereal Sci, 2002, 35 : [ 2 ],,,.., 2005, 38 (9) : [ 3 ] Saulnier L, Marot C, Chanliand E, et al. Cell wall polysaccharide interactions in maize bran. Carbohydr. Polym, 1995, 26 : [ 4 ] Girhammar U, Nair B M. Isolation, separation and characterisation of water soluble non2starchy polysaccharides from wheat and rye. Food Hydrocolloids, 1992, 6 : [ 5 ] Bergmans M F, Gruppen H G. Optimisation of the selective extraction of glucronoarabinoxylans from wheat bran : use of barium and clcium hydroxide solution at elevated temperatures. Journal of Cereal science, 1996, 23 : ( 104 )

6 104 Journal of Nuclear Agricultural Sciences 2008,22 (1) : ,,,,,,,, 40Gy,,,,,,,,, [6 ],,,,,,,,,,, 40Gy,,,,,,,,,,,,, : [ 1 ].., 1998, (3) :41 45 [ 2 ],,,..,2004,41 (2) : [ 3 ],,,..,2005,42 (3) : [ 4 ],,,..,2006, 26 (2) : [ 5 ]..,1998,12 (2) : [ 6 ],,,..,2005 [ 7 ],,. 60 Co Dacus citri., 1990,4 (2) : [ 8 ],,. 60 Co Dacus citri., 1991,5 (2) :79 82 [ 9 ],,,. F21., 2002,16 (4) : [10 ],,,..,2000, 14 (1) : [11 ],,,.., 2003, 16 (1) : [12 ] Lu Daguang. The feasibility of control of anoplophora glabripennis with sterile insect chinque. Acta Agriculture Nucleatae Sinica, 2001, 15 (5) : [13 ] Bartlett A C. Genetic changes during insect domestication. Advances and challenges in insects rearing, ARS, USDA New Orlean, 1984, 1 8 ( 64 ) [ 6 ] Schooneveld2Bergmans M F. Structural features of glucuronoarabinoxylans extracted from wheat bran by barium hydroxide. Journal of Cereal Science, 1999, 29 : [ 7 ] Hofmann R, Roza A. Structural characteristics of the cold water soluble arabinoxylans from the white flour of the soft wheat variety kadet. Carbohydrate Polymers, 1992, 15 : [ 8 ] Fincher G B, Stone B A. Cell Walls and Their Components in Cereal. Grain Technology, 1986, 7 : [ 9 ] Landis W D, Hoa K C, Marshall L. Improved Process for Isolation of Corn Fiber Gum. Cereal Chem, 1998, 75 (4) : [10 ],.. :, 1997, [11 ] Hashimoto S, Shogren M D, Pomeranz Y. Cereal Pentosans : Their Estimation and Significance I Pentosans in Wheat and Milled Wheat. Products. Cereal Chem, 1987, 64 (1) :30 34 [12 ].., 2002,12

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