Differences in Contents of Neutral Aroma Components and Sensory Evaluation in Air-cured Burley Leaves from Different Curing Barns

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1 > > > > > > > > > > > > > > > S572 A Differences in Contents of Neutral Aroma Components and Sensory Evaluation in Air-cured Burley Leaves from Different Curing Barns SHI Hong-zhi 1 YANG Xing-you 2 ZHOU Kai-xu 2 LI Hao 1 ZHAO Yong-li 1 ZHANG Ding-gui 2 XIE Zi-fa 2 1. National Tobacco Cultivation & Physiology & Biochemistry Research Center of Henan Agricultural University Zhengzhou China 2. Dazhou Tobacco Research Institute Dazhou China Abstract GC /MS was used to analyze the contents of neutral aroma components for upper and middle stalk position air-cured leaves from 6 different curing barns and experimental cigarettes were made to conduct sensory evaluation. Results showed that the total contents of neutral volatile aroma components for middle position leaves followed the order of bamboo-mud barn > black plastic with grass barn > all-black plastic barn > plastic covering structure > wooden plate barn > tile-roofed barn and for upper position leaves the order was bamboo-mud barn > black plastic with grass barn > plastic covering structure > wooden plate barn > all-black plastic barn > tile-roofed barn. When neophytadiene was deducted from the total the lowest level was for plastic curing structure. High contents of carotenoid catabolites megastigmatrieonel β-damascone et al and cembratriendiol catabolite solanone in both stalk position leaves and phenylalanine and sugar catabolites in upper stalk position leaves were found for bamboo-mud barn and black plastic with grass barn. Lower levels of aroma components in middle stalk position leaves and carotenoid catabolites in upper stalk position leaves were found for wooden plate barn and tile-roofed barn. The lowest content of solanone was found in cured leaves from plastic covering structure and all-black plastic barn. The sensory evaluation score followed the order of bamboo-mud barn > black plastic with grass barn > all-black plastic barn > plastic covering structure > wooden plate barn > tile-roofed barn

2 Key words Burley Barn Neutral aroma components Sensory evaluation d 60% ml g g 500 μl 302 mg /L 350 ml 40 ml 250 ml 2. 5 h 250 ml 10 g 1 ml 6 1 GC /MS NIST HP5890Ⅱ-5972 GC /MS HP-5 60 m mm. i. d μm d. f. He 0. 8 ml /min min 5 /min min 5 /min min /min min L 70 ev EI 50 ~ hm amu NIST hm 2 13 kg kg 25 kg 150 kg kg 20 kg 1 1 ~ /hm

3 β- β β- β- Tab. 1 1 Effect of different curing barns on neutral aroma component contents in middle stalk leaves of burley tobacco Curing barns Components All-black Tile-roofed Bamboo-mud Black plastic Plastic covering Wooden plastic barn barn barn with grass barn structure plate barn Furfural Furfural alcohol Acetyl furan Methyl-2-furfural Benzyldehyde Methyl-5-hepten-2-one Methyl-5-hepten-2-ol dimethyl-2 5-furandione Benzyl furan Phenyl acetaldehyde Linalool Phenyl ethyl alcohol Keto-isophorone Indole Solanone β- β-damascone Geranyl acetone Dihydroactinidiolide Megastigmatrieonel Megastigmatrieonel β- 3-Hydroxy-β-dimascone Megastigmatrieonel a- 3-Oxo-a-ionol Solavetivone Neophytadiene Famesyl aectone Total > > > > > > > > > > > > > > > > > > > > μg / g

4 Tab. 2 2 Components Effect of different curing barns on neutral aroma component contents in upper stalk leaves of burley tobacco μg /g Curing barns All-black Tile-roofed Bamboo-mud Black plastic Plastic covering Wooden plastic barn barn barn with grass barn structure plate barn Furfural Furfural alcohol Acetyl furan Methyl-2-furfural Benzyldehyde Methyl-5-hepten-2-one Methyl-5-hepten-2-ol dimethyl-2 5-furandione Benzyl furan Phenyl acetaldehyde Linalool Phenyl ethyl alcohol Keto-isophorone Indole Solanone β- β-damascone Geranyl acetone dihydroactinidiolide Megastigmatrieonel Megastigmatrieonel β- 3-Hydroxy-β-dimascone Megastigmatrieonel a- 3-Oxo-a-ionol Solavetivone Neophytadiene Famesyl aectone Total β β

5 3 117 Tab. 3 3 Sensory evaluation scores for experimental cigarettes made from middle leaves from different curing barns Barns Style Aroma Aroma Strength Density After-taste Odor Total 10 quality 10 quantity All-black plastic barn Tile-roofed barn Bamboo-mud barn Black plastic with grass barn Plastic covering structure Wooden plate barn M M Sakari T. Analysis of the headspace volatiles of tobacco u- sing an ether trap J. Agric Biol Chem M J J J β- 8. J J J J ph J J J J J J

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