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7 T Nakasone et al : Development of HIV Infectious Diseases Integrated Database + : HIV,* : -/0-0* +33.., : HIV HIV BCG -0 :.-/ /. - Yamanaka T, Fujimura Y, Ishimoto S, Yoshioka A, Konishi M, Narita N, Mimaya J, Meguro T, Nakasone T, Okamoto Y, Yoshizaki H, Yamada K, Honda M : Correlation of titer of antibody to principal neutralizing domain of HIV MN strain with disease progression in Japanese hemophiliacs seropositive for HIV type +. AIDS Res Hum Retrovir +- : -+1 -,0, Yasuda S, Iwasaki M, Oka S, Naganawa S, NakasoneT, Honda M, Sata T, Kojima A, Matsuda S, Takemori T, Tsunetsugu-Yokota Y : Detection of HIV-gag p,.- specific antibodies in sera and saliva of HIV-+-infected adults and in sera of infants born to HIV-+-infected mothers. Microbiol Immunol., : -*/ -++, / NakasoneT, Totani R, Yamazaki S, Honda M : HIV-+ subtypea in Japan. AIDS +, : 3/* 3/,, Yoshino N, Naganawa S, NakasoneT, Imura S, National Cooperative Study Investigators on Vertical Transmission of HIV-+, Kita T, Honda M : Vertical transmission of human immunodeficiency virus type + (HIV-+) in Japan, : presence of two subtypes B and E with subtype E predominance. Acta Pediatrica Japonica.* : /*- /*2, NakasoneT, Takamatsu J, WatanabeK, Naganawa S, Someya K, Yoshino N, Kaizu M, Ohsu T, Takizawa M, Izumi Y, Kawahara M, Hara T, Fujimura Y, Yamada K, Nagai Y, Yamazaki S, Honda M : Decline in the HIV-+ isolation ratein Japan : A +, year observation. Microbiol Immunol.. : 3.3 3/,,,***. 2 Hara T, Yoshino N, Takayama N, Minamitani M, Naganawa S, Ohkubo H, Takizawa M, Izumi Y, KantakeM, Suzuki S, Takano M, Kita T, Totani R, Nagai Y, Honda M, Nakasone T : Presence of multiple HIV type + subtypes among mothers and children in Japan. AIDS Res and Hum Retrovir +1 : /03 /1/,,**+. 3 Naganawa S, Sato S, Nossik D, Takahashi K, Hara T, Tochikubo O, Kitamura K, Honda M, NakasoneT : First report of CRF*-_AB recombinant HIV type + in injecting drug users in Ulraine. AIDS Res and Hum Retrovir +2 : ++./ ++.3,,**,. +* http : // ++ http : // gensoku.htm +, Los Alamos HIV Database http : //hiv-web. lanl.gov/content/index.2.2

8 The Journal of AIDS Research Vol. 0 No. +,**. Development of HIV Infectious Diseases Integrated Database Tadashi NAKASONE +, Takashi HARA +),,, Kenji SOMEYA +,,, Kazuho IKEO -, Takashi GOJOBORI -, Naoki YAMAMOTO + and Mitsuo HONDA +) + AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan, Japan Science and Technology Agency, Tokyo, Japan - CenterforInformation Biology and DNA Data Bank of Japan, Shizuoka, Japan Objective : In order to make the wide use of the accumulated data for the development of HIV/AIDS vaccines, we developed the HIV Infectious Diseases Integrated Database (HIV-DB ; URL https : //aids.nih.go.jp) and made it available to any HIV-related researchers via Internet. Outline : The HIV-DB provides virological and genetic data, such as results of virus isolation, nucleotide sequences information on HIV-+ and structural information on the virus proteins, integrated with indispensable clinical data, which have been collected and analyzed at the National Institute of Infectious Diseases (NIID) since Contents : The HIV-DB includes the HIV DNA database of the DNA Databank of Japan (DDBJ), results of virus isolation (n -,,+1), C,V- DNA data (n -0+), V- loop structural data (PDB format, n,03), and corresponding clinical data (birth year, sex, CD. lymphocyte count, plasma virus lord, drug history, etc.), which have been obtained from /1, HIV- +-infected individuals in Japan. Several functions including FASTA similarity search analysis against the HIV DNA database of DDBJ, phylogenetic tree analysis, genosubtyping of HIV- +, creation of individual user divisions, browsing -D view of V- loop structure and search for corresponding clinical data, are also available on the WEB site. Remarks : The HIV-DB was developed by collaboration of NIID and the Japan Science and Technology Corporation. Key words : HIV infections diseases, database, HIV gene, HIV protein -D structure.3.3