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1 a b *2**/1, 302/,**- * -*,**.,,,., BMS No. 1/ (,) :0/11,,**.. -.*,**- - -,**- / 0,***,**,,,.,,. CLM,, : / : : : : a,312/-- b **23/* : fax : *//..,,/, s*yamada@nlbc.go.jp 1/, : 0/11,,**. 0/

2 CLM CLF ,***,3 /*. 331, * 333 1, /22,,** / 3,**, 3,/ /* ,**, *,** / 3,**,, 2 / ,/,,**-, - 1.* m3.* m TDN1-.*...3 TDN 1,.*./,../ kg., 2 / 01 WinRoof 0 01 / cm */,. 0 1* -* Waner-Bratzler. 1 CV, kg, / kg 131.*3.3 kg CV,.. -.-,./, ,03.*..1 kg CV/./, kg CV-.3 /2*./-.3 kg CV,.. /1/.,.1.1 kg CV2.- CV..,3 kg -*.,0 kg 1/, : 0/11,,**. 00

3 Table. Date of birth, birth weight and body weight of the clones and the donor during fattening period Body weight (kg) Date of birth Birth weight starting, the middle at the start of period - at the start of the latter period. finishing / CLM */,2/32 3//** 3/,/** 3/,/** 3/,/**, /,./,3,,3.,11,10.2 /* /0 /*. /*, 0/ 1** 1*0 03* 03, 11 12, 2** 12, 2,. Average of clone -342,2.41 /*24* 0314* 1314* Di#erence.43,4... -/ 3 34/ -4-,3., , /0 /*, *0 03* 0 343,4/ 2,. 12,.,,/2/31 1/-/** 1/./**,*..,3 3. -*.,0 --* * 0-0 /-, 0, *3 Average of clone -04/,2,41./04* /2.4* Di#erence /.- 2* *. 2* Di#erence means the di#erence between maximum and the minimum value in the steer, and the di#erence of two clones in the heifer., The starting time of fattening was * day. - The start day of the middle period was 02th day from the start of fattening.. The start day of the latter period was --0th day from the start of fattening. / The finishing time were /*.th day in steer and /22th day in heifer from the start of fattening *3 kg DG, DG DG CV.2. CLM,,.*..2 DG 321 *.20*.*0 kg CV *.32 *. kg CV.,- CV DG *.,, kg *.. kg *.-* kg *.,- kg - /..1 cm.0..., cm CV.. * ,./-.. cm CV,.0 1/, : 0/11,,**. 01

4 Fig.. Increase of body weight of clone (a) steer, (b) heifer, observed during fattening period...0 *., cm *.2 cm,.,**, 2.,,**,, 3, kg,,,* kg 1/ 02 kg, DG *.20 *.2, kg 1/, : 0/11,,**. 02

5 Table,. Daily gain of the clones and the donor during fattening period Daily gain (kg/day) in the first period, in the middle period, in the latter period - total 4-* 4-, 4-/ CLM 4* *43- *4.3 *4/0 *4// *413 4*. *431 4** 4** 4*3 Average of clone *40* 4*, Di#erence *4*, 42 4-/ 4-* *4*/ *4* *4*, *4-,,4* *413 *4.3 *4-* *4*/.42 4*3 *431 *4 *42 4. *43, *4/3 *40 *4- *423 *42- *403 *41. *42- *4/3 Average of clone 4*- *410 *4.0 *41 Di#erence *4,, *4. *4-* *4,- Di#erence means the di#erence between maximum and the minimum value in the steer, and the di#erence of two clones in the heifer., Both of the first period and the middle period were for 02 days. - The latter periods were for 02 days in steer and,/, days in heifer. DG,./,2/ kg kg.* kg,2 kg 3.,2- kg 0,3 033 kg 23 kg 1* kg DG.* /. cm..*.0.. cm *1.*,.. cm -* cm,, 1/, : 0/11,,**. 03

6 Table -. Withers height of the clones and the donor during fattening period Withers height (cm) starting, the middle at the start of period - at the start of the latter period. finishing / CLM 4* /42 04,,40 /40,.4* * -*4* -,4. -.4, *4*.40.4*.04*.14, Average of clone /4 -,4/ Di#erence ,, * -*4*.4* *4* -42 4, * ,40 *14*.40 *4,,*4,,140,,40,14* --4*,140 -* *42 Average of clone,4.,/4 -* Di#erence.4. /4* /4. 14* Di#erence means the di#erence between maximum and the minimum value in the steer, and the di#erence of two clones in the heifer., The starting time of fattening was * day. - The start day of the middle period was 02th day from the start of fattening.. The start day of the latter period was --0th day from the start of fattening. / The finishing times were /*.th day in steer and /22th day in heifer from the start of fattening.,** -. -,. / CLM DG CV 1.* /.*,,./ kg *. kg 1/, : 0/11,,**. 1*

$z{! Fig.,. Side views of. clone steers and, clone heifers at ﬁnishing. PQRS ^_` CV 3 PQ4 3,.1ῌ abcd ef3 0.ῌ BMS No. 3,..ῌ g h#3../ῌ ijkl h#3 /.1ῌ M\ PQ4 abcde f g h# CV 3F#m BMS No.$

7 z{! Fig.,. Side views of. clone steers and, clone heifers at ﬁnishing. PQRS ^_` CV 3 PQ4 3,.1ῌ abcd ef3 0.ῌ BMS No. 3,..ῌ g h#3../ῌ ijkl h#3 /.1ῌ M\ PQ4 abcde f g h# CV 3F#m BMS No. ijkl nomg pq$r ' ] * Ns E3 PQ Ntu -0/ kg abcdef 3 1. Ntu /- cm, BMS No. 3 * Ntu 2 g h #3 3., Ntu 1.* cm ijkl h#3.* Ntu,.0 cm M\ v Q w0 _` E$ ' Q w0 xy E3 z{ }~ Ns { E z{2 $ { 9 t ' = BMS No. E3 ] * Y Z *3K M ' j,**, 3, [! "#$%&# ' () *,-./ : 4 ( ) ;< =$>?@ 3AB C23() =$;< 9 : D2 E3F#B G ' >?HIJ 3123K L M $ C2 NB () $": 9 : () =$O B ' -ῌ ῐ PQRS TU V 0 % ' PQWX3 YZ * 3 WX3 A/ $, [ A. $, [ M\ ] * 3 WX3 A/ $ [ A. $ [ M ' YZ * 1/, : 0/ῌ11,,**. 1

8 Table.. Amount of concentrated feed intake of the clones and the donor during fattening period Feed intake (kg/head) in the first period, in the middle period, in the latter period - total 2-0 5*13 5* 5*/2 5*/. 5/- 5.2, * 5.,. 5/* CLM /,- 5., 5/,* ** -53.* Average of clone 5* ,.5**2 Di#erence,,,4* 5* 5*/..1, , 5.,. /2 /2-43 5/,- 5., ** -53.* 0* 200 5,2 5*-2 5,.0 5/,2 5,. 522-,5/* 5, /.523/ -5//, Average of clone 5, ,.5,,. Di#erence 2, Di#erence means the di#erence between maximum and the minimum value in the steer, and the di#erence of two clones in the heifer., Both of the first period and the middle period were for 02 days. - The latter periods were for 02 days in steer and,/, days in heifer kg /2 /0 cm, BMS No. 1 3, 2.1 cm, -.- cm BMS No. BMS No.,. 33/ *..,*.. kg BMS No. *.. cm BMS No. Mannen 332 DNA BMS DNA DNA DNA BMS No. 1/, : 0/11,,**. 1,

9 Table /. Feed conversion ratio of concentrated feed of the clones and the donor during fattening period Feed conversion (kg/head) in the first period, in the middle period, in the latter period - total /4* / 340 CLM 14/ 04, /4-4/ Average of clone /4 142 Di#erence,40 14* 14/ 4/ 04* *4, -4/ /4*.41 *4- *4,, / *4. *4. /4* * *43 *41,40.4* 04/ 34, *4 *4, Average of clone 04/ *42 /4- *4 Di#erence * *4. *4, 4. *4, 41 4.,4/ Di#erence means the di#erence between maximum and the minimum value in the steer, and the di#erence of two clones in the heifer., Both of the first period and the middle period were for 02 days. - The latter periods were for 02 days in steer and,/, days in heifer. *4 *42 *4 BMS No. BMS No. - 1 CV 2., ,**- / CV,.2...,**,, 33-1/, : 0/11,,**. 1-

10 Table 0. Carcass characteristics of the clones and the donor Carcass weight (kg) Grade Rib eye area (cm, ) BMS No. Rib thickness (cm) Subcutaneous fat thickness (cm) A-. A-. A-/ A-. A-/.2 / /..2 // / CLM /, ,4, 40 Average of clone.3/4* /,4* / 42 Di#erence -4, 04 //.2 1 4*,4. 3 1, *4..4/ *42-4-,41 /,.2-,3 *4- /41,4, 40 * / A-/ A-/ A-. /3 1. /- 3 * 2 14* 34, 14*,4* 4*,40 Average of clone.,1 0-4/ 34* Di#erence,.,,,4, 40 Di#erence means the di#erence of maximum and the minimum value by the steer, and the di#erence of two clones by the heifer.. 2 CV,./ *./ /- 0.32/.2 CV CV.*., 2.-,.1 CV CV*./ BMS No. CV,.. CV 2.* 33. BMS No. * 2 -.., -../- 1/, : 0/11,,**. 1.

11 Fig. -. Cross sections of carcass of. clone steers,, clone heifers and the donors. Table 1. Measurements of M. longissimus thoracis (ribeye) at cross-section between the 0th and the 1th ribbone of clones Length of major axis (cm) Length of minor axis (cm) Circumference length (cm) CLM CLM 34./ /* 14,24* **,34/3-4,0,241/ -*41* Average of clone 34.* 14/, -*4*1 *4,3-4 *40, 24, 4, -41 1/ ῌ, : 0/ῌ11,,**. 1/

12 Table 2. Physiological chemistry analysis of M. longissimus thoracis of the clones and the donor Moisture () Ether extract () Crude protein () Cooking loss () Shear force value (lb/cm, ) Water holding capacity () /*432 /, /4*,.04,0 -,421-4*/ -14/ * CLM /40- / /3-433,.41,,4.0,/4,,,4,/.4* * *4,2 2,40, /43 Average of clone /4.-.4/0, ,4/* Di#erence,42. /41 /,412.04,0 04/, -41- *4/ -340* -4*/ 24/0 *413 /4/ / *,/4,,,4,/ -431 *4-24*.4*1-4-1 *41*,4*.,4/ 2/43 2*4,2.43 /4*. /*4*/ , , -.4/- /40. /4,0 /4*2,-41,, ,.4-* 134,, 2*4/* Average of clone /41,-41*.4/ Di#erence *42 *4-, *42 *4*/ *4/, 4,2 Di#erence means the di#erence between maximum and the minimum value in the steer, and the di#erence of two clones in the heifer. 33-,3 :. 323, :../..,**- 1. : 1. Mannen H, Kojima T, Oyama K, Mukai F, Ishida T, Tsuji S E#ect of Mitochondrial DNA Variation on Carcass Traits of Japanese Black Cattle. Journal of Animal Science, 10 : -0..,**-,** / 0 :..,**, -/ :,2.*.,** -* : // : / /,2 : 13.,***,. 33. ***. 1/, : 0/11,,**. 10

13 Evaluation of Similarity in Growth and Carcass Characteristics of Somatic Cell d Beef Cattle Shinichi YAMADA, Atsushi NISHIYAMA, Tadashi KAWAMURA, Yuji GOTO, Toshihiro NADE, Toshiaki OKUMURA and Kenji YAMAUCHI National Livestock Breeding Center Tokachi Station, Otofuke-cho, Hokkaido *2**/1,, Japan National Livestock Breeding Center, Nishigo-mura, Fukushima-ken 302/, Japan Corresponding : Shinichi YAMADA (fax : 2 (*) //..,,/, s*yamada@nlbc.go.jp) Somatic cell cloned cattle of Japanese Black were fattened under the same condition, and similarity in growth and carcass characteristics were examined. Four somatic cell cloned steers and two somatic cell cloned heifers which were born at almost the same period were fattened according to similar fattening method of the donor. The clones of respective groups were compared with one another and with their donor on growth, feed intake, carcass characteristics and the physiological chemistry analysis of the M. longissimus thoracis. In each item, high similarity was recognized in the cloned steer, while di#erences in growth and meat quantity traits were recognized in the cloned heifer due to illness. Between the clones and the donor, there was an environmental factor by the di#erence in fattened time and place which caused the di#erence in the growth. On the other hand, similarity was suggested in BMS No. and ether extract of M. longissimus thoracis. High similarity was recognized among fattened somatic cell clones, although growth and meat quality traits might be a#ected by the condition of the clones. Nihon Chikusan Gakkaiho, 1/ (,) :0/11,,**. Key words : Somatic cell clone, Similarity, Beef cattle, Fattening 1/, : 0/11,,**. 11

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