Archive of SID. نشانمند كردن ژنه يا برگرداننده باروري بوسيله تجزيه كلاس مغلوب در برنج چكيده مقدمه

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1 نشانمند كردن ژنه يا برگرداننده باروري بوسيله تجزيه كلاس مغلوب در برنج (-), ياراحمدي محمد مهدي سوهاني * ابوبكر جوهرعلي بابك ربيعي (// : -// : ) سعيد و ع يل 5 اكبر عبادي چكيده WA. IR42686R IR58025A 3. / / (WA) RM271 RM6737 RM315 RM / / / RM /.. CMS/ واژهه يا كليدي:..1988). 1.(Ahmadikhah & Karlov, 2006) % WA مقدمه. -/.. 1. Wild abortive (Virmani & Wan, (CMS/) msohani_99@yahoo.com - : : *

2 /). ( ;(Govinda & Virmani, 1988; Wang, ) (Bharaj et al., 1995; Zhou, 1983) WA 1980) ( %/. (Pei, 1980). ( ). (IRH1).. %. (Allard, 1956). LOD.. :: :( ). ( ) :( ) LOD=3 ( LOD ) ( ). 1. Kosambi.(Allard, 1956) 1 مواد و روشها F 1 IR58025A/IR42686R.. (IRRI) IR36. (). I 2 -KI. % DNA. CTAB.(Murray & Thompson, 1990) (PCR).

... :. / C234. G4003 (6) / G4003 (1998) Nguyen et al.. RG140 RAPD OPW0L-350 (1998) Zhang et al. OPU10-100 OPK05-800 LP 3.. 3 6 RM591 RM271 RM6737 RM258 / / / / (2006) Ahmadikhah, & Karlov.

3 ... :. / C234. G4003 (6) / G4003 (1998) Nguyen et al.. RG140 RAPD OPW0L-350 (1998) Zhang et al. OPU OPK LP RM591 RM271 RM6737 RM258 / / / / (2006) Ahmadikhah, & Karlov.() 6 RM6737 RM171 / / 6 RM6737 /. نتايج و بحث /.(Dorosti et al., 2006)..(Jing et al., 2001; Zhang et al., 1994).. LOD. RM315 RM443 3 / / (1997) Yao et al..() 4 3 P 1P 2A A A A A A A R A A A A H A A A A A H A A A A H A A A A A A H A A A A A A A A A H A A A A P 2 P 1. PCR -. R H

4 - : : - χ 2 * (/) (/) (/) / (/) (/) (/) / (/) (/) (/) / (/) () (/) / (/) () (/) / (/) (/) (/) / (/) (/) (/) / () () () / (/) (/) (/) / (/) () (/) / (/) () (/) / () () () / (/) (/) (/) / RM17 (/) (/) (/) / (/) () (/) / RM443 RM315 RM237 RM505 RM6344 RM4098 RM258 RM591 RM271 RM6737 RM3019 RM7003 RM3226 :* % : : LOD - LOD* RM443 3 / / / RM315 3 / / / RM / / / RM258 6 / / / RM591 6 / / / RM271 6 / / / RM / / / 7 / / / RM / / / HL RM RM1108 6(t). SBD07 QTL. RM5373 RM5373 RM6737 (1998) Tan et al. بازگرداننده C LOD :* 1 (2004) Tao et al.. Dian1 RM6100 RM171 / /. (2004) Liu et al. ( 5 6(t))

5 ... : / / QTL. %/ RM7003.() %/. (2001) Jing et al. RG257 R SSLP WA... Basmati ) IR42686 IRRI. RM228 Zhehan97A RM171 IR24 4. / / RM244 IR64 (2003) Mishra et al.. RM258 / Huang et al.. /. RM171 HL (2000). / / HL (1996) Akagi et al. RM258. / 4 RM6344 RM171 / (1995) Bharaj et al..(). Yoshimura et (1). Ram Tulasi, Mohan, Bhog, IR Zehan97A IR Basmati T3 IR (Haa Raj IET 4888 IR IR48 2 IR36. IR58025B IR58025A. WA WA-2 -WA-1. Boro (1982) al..

6 . 4 سپاسگزاري. ().. / 7. / RM6344 REFERENCES 1. Ahmadikhah, A. & Karlov, G. I. (2006). Molecular mapping of the fertility-restoration gene 4 for WA-cytoplasmic male sterility in rice. Plant Breeding, 125, Akagi, H., Yokozeki Y., Nakamura, A. & Fujimura, T. (1996). A codominant DNA marker closely linked to the rice nuclear restorer gene, -1, identified with inter-ssr fingerprinting, Genome, 39, Allard, R. W. (1956). Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia, 24, Bharaj, T. S., Virmani, S. S. & Khush, G. S. (1995). Chromosomal location of fertility restoring genes for WA abortive cytoplasmic male sterility using primary trisomics in rice. Euphytica, 83, Dorosti, H., Ali, A. J., Nematzadeh, G., Ghodsi, H., Allahgholipour M., Nouri, M. Z., Vallizadeh, A., Nahvi, M., Karbalai, M., Eefani, A. R. & Alinia, F. (2006). IRH1-the first aromatic hybrid rice in Iran. International Rice Research Notes, 31, Govinda, R. K. & Virmani, S. S. (1988). Genetics of fertility restoration of WA type cytoplasmic male sterility in rice. Crop Science, 28, Huang, C. S, Tseng, T. H. & Liu, C. (1986). Inheritance of fertility restoration of cytoplasmic male sterility in indica rice. In: Rice genetics, International Rice Research Ititue, Manila, Philippines. Pp Huang, Q. Y., He, Y. Q., Jing, R. C., Zhu, R. S. & Zhu, Y. G. (2000). Mapping of the nuclear fertility restorer gene for HL cytoplasmic male sterility in rice using micro-satellite markers. Chinese Science Bulletin, 45, Jing, R., Li, X., Yi, P. & Zhu, Y. (2001). Mapping fertility-restoring genes of rice WA cytoplasmic male sterility using SSLP markers. Botanical Bulletin of Academia Sinica, 42, Liu, X. Q., Xu, X., Tan, Y. P., Li, S. Q., Hu, J., Huang, J. Y., Yang, D. C., Li, Y. S. & Zhu, Y. G. (2004). Inheritance and molecular mapping of two fertility-restoring loci for Honglian gametophytic cytoplasmic male sterility in rice (Oryza sativa L.). Molecular Genetics and Genomics, 271, Mishra, G. P., Singh, R. K., Mohapatra, T., Singh, A. K., Prabhu, K. V., Zaman F. U. & Sharma R. K. (2003). Molecular mapping of a gene for fertility restoration of wild abortive (WA) cytoplasmic male sterility using a basmati rice restorer line. Journal of. Plant Biochemistry & Biotechnology, 12, Murray, M. G. & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, Nguyen, T. L., Zhang, G., Magpantay, G., Virmani, S. S., Huang, N., Brar, D. S., Kush, G. S. & Li, Z. K. (1998). PCR-based DNA markers for the WA-CMS fertility restoring gene -3 in rice. Rice Genetics Newsletter, 15, Pei, X. S (1980). A new view on inheritance of male sterility in plant. Agricultural Science and Technology of Hunan, 3, Tan. X. L., Vanavichit, A., Amorilpa, S. & Tragoonrung, S. (1998). Genetics analysis of rice CMS- WA fertility restoration based on QTL mapping. Theoretical and Applied Genetics, 96, Tao. D., Xu, P., Li, J., Hu, F., Yang, Y., Zhou, J., Tan, X. & Jones, M. P. (2004). Inheritance and mapping of male sterility restoration gene in upland japonica restorer lines. Euphytica, 138(3), Virmani, S. S. & Wan B. H. (1988). Development of CMS lines in hybrid rice breeding, Pp in: Hybrid rice. International Rice Research Ititue, Manila, Philippines. 18. Wang, S. L. (1980). Study on inheritance of restoring factor and approach of developing new restorer in rice. Agricultural Science and Technology of Hunan, 4, 1-4.

7 ... : 19. Yao, F. Y., Xu, C. G., Yu, S. B., Li, J. X., Gao, Y. J., Li, X. H. & Zhang, Q. (1997). Mapping and genetic analysis of two fertility restorer loci in the wild-abortive cytoplasmic male sterility system of rice (Oryza sativa L.). Euphytica, 98, Yoshimura, A., Iwata, N. & Omura, T. (1982). Linkage analysis by reciprocal tralocation method in rice plants (Oryza Sativa L.). III. Marker genes located in chromosomes 2, 3, 4, and 7. Japanese Journal of Breeding, 32, Zhang, G., Bharaj, T. S., Virmani, S. S. & Huang, N. (1997). Mapping of the 3 nuclear fertility restoring gene for WA cytoplasmic male sterility in rice using RAPD and LP markers. Theoretical and Applied Genetics, 94, Zhang, Q., Shen, B. Z., Dai, X. K., Saghai Maroof, M. A. & Li, Z. B. (1994). Using bulked extemes and recessive class to map genes for photoperiod-seitive genic male sterility in rice. In: Proceedings of the National Academy of Sciences. USA, 91, Zhou, T. (1983). Analysis of R genes in hybrid indica rice of WA type. Acta Agronomica Sinica, 9,

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