SSR, SSR Ana lysis of Genetic D iversity of Pear Cultivars

Σχετικά έγγραφα
Evaluation of Resistance to Scab in Pear Germplasms

SSR. : SSR ( Simp le sequence repeat) 29 ( ), 6 SSR (BGA35 KU10 BGT23b NH004a NH011b NH015a) NH004a, 3, 6

Error ana lysis of P2wave non2hyperbolic m oveout veloc ity in layered media

Identification of Fish Species using DNA Method

Genetic Rela tion sh ip of Som e Cultivars of Petun ia Hybr ids Using SRAP M arker

A Preliminary Analysis of Genetic Relationships for Prunus mume Sieb. et Zucc. by AFLP

( ) , ) , ; kg 1) 80 % kg. Vol. 28,No. 1 Jan.,2006 RESOURCES SCIENCE : (2006) ,2 ,,,, ; ;

Effects of Plan t Growth Regula tors on the Con ten t of Phenolics in Sweet Cherry D orman t Flower Buds and D ormancy

ΤΕΧΝΟΛΟΓΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΚΥΠΡΟΥ ΣΧΟΛΗ ΓΕΩΤΕΧΝΙΚΩΝ ΕΠΙΣΤΗΜΩΝ ΚΑΙ ΔΙΑΧΕΙΡΙΣΗΣ ΠΕΡΙΒΑΛΛΟΝΤΟΣ. Πτυχιακή εργασία

cm cm 92. 0% DH S 917

SAMPL ING STRATEGY FOR PRESERVATION OF GERM PLASM FROM DAVIDIA INVOLUCRATA

Jie He. To cite this version: HAL Id: halshs

ΠΑΝΕΠΙΣΤΗΜΙΟ ΠΑΤΡΩΝ ΤΜΗΜΑ ΔΙΟΙΚΗΣΗΣ ΕΠΙΧΕΙΡΗΣΕΩΝ ΜΕΤΑΠΤΥΧΙΑΚΟ ΠΡΟΓΡΑΜΜΑ ΜΒΑ

Approximation Expressions for the Temperature Integral

Accumulation of Soil Arsenic by Panax notoginseng and Its Associated Health Risk

Genetic study of 46 Greek grape cultivars by random amplified polymorphic DNA markers (RAPD- PCR). K. Biniari and M.N.Stavrakakis

Studies on the Binding Mechanism of Several Antibiotics and Human Serum Albumin

Antimicrobial Ability of Limonene, a Natural and Active Monoterpene

«Συντήρηση αχλαδιών σε νερό. υπό την παρουσία σπόρων σιναπιού (Sinapis arvensis).»

30s 56 60s 72 60s dntp cm s s s 23

The toxicity of three chitin synthesis inhibitors to Calliptamus italicus Othoptera Acridoidea

, Litrrow. Maxwell. Helmholtz Fredholm, . 40 Maystre [4 ], Goray [5 ], Kleemann [6 ] PACC: 4210, 4110H

,,, (, ) , ;,,, ; -

Analysis on the Ratio of Flesh Content and Nutritional. Quality of Esox lucius

DNA, , DNA, 1 D NA . DNA

HIV HIV HIV HIV AIDS 3 :.1 /-,**1 +332

Optimizing Microwave-assisted Extraction Process for Paprika Red Pigments Using Response Surface Methodology

ΠΑΝΔΠΗΣΖΜΗΟ ΠΑΣΡΩΝ ΣΜΖΜΑ ΖΛΔΚΣΡΟΛΟΓΩΝ ΜΖΥΑΝΗΚΩΝ ΚΑΗ ΣΔΥΝΟΛΟΓΗΑ ΤΠΟΛΟΓΗΣΩΝ ΣΟΜΔΑ ΤΣΖΜΑΣΩΝ ΖΛΔΚΣΡΗΚΖ ΔΝΔΡΓΔΗΑ

LUO, Hong2Qun LIU, Shao2Pu Ξ LI, Nian2Bing

RAPD. ( B rassica rapa) RFL P ( Restriction Fragment Length Polymorphism) RFL P ; RFL P, rapa. CHIN ESE BIODIV ERSIT Y 6 (2) :99 104, May, 1998 RAPD

A summation formula ramified with hypergeometric function and involving recurrence relation

IL - 13 /IL - 18 ELISA PCR RT - PCR. IL - 13 IL - 18 mrna. 13 IL - 18 mrna IL - 13 /IL Th1 /Th2

Σχέση µεταξύ της Μεθόδου των ερµατοπτυχών και της Βιοηλεκτρικής Αντίστασης στον Υπολογισµό του Ποσοστού Σωµατικού Λίπους

(A ntifreeze p roteins, A FP s) (afp ),

ΕΠΑΝΑΛΗΨΗ ΨΕΥΔΟΛΕΞΕΩΝ ΑΠΟ ΠΑΙΔΙΑ ΜΕ ΕΙΔΙΚΗ ΓΛΩΣΣΙΚΗ ΔΙΑΤΑΡΑΧΗ ΚΑΙ ΠΑΙΔΙΑ ΤΥΠΙΚΗΣ ΑΝΑΠΤΥΞΗΣ

Study on Wheat Heterotic Group

Elucidation of fine-scale genetic structure of sandfish (Holothuria scabra) populations in Papua New Guinea and northern Australia

J. of Math. (PRC) Banach, , X = N(T ) R(T + ), Y = R(T ) N(T + ). Vol. 37 ( 2017 ) No. 5

Quick algorithm f or computing core attribute

Καρδιακή Συχνότητα και Πρόσληψη Οξυγόνου Ατόμων Μέσης Ηλικίας κατά την Εκτέλεση Ελληνικών Παραδοσιακών Χορών

D etection on the Sen sitiv ity of Pota to Phytoph thora infestans to M eta laxyl and Cym oxan il

Ελληνικό Περιοδικό Διοίκηση Αθλητισμού & Αναψυχής, 11 (2), 24 35, Δεκέμβριος, τόμος 11 (2), Δημοσιεύτηκε: Δεκέμβριος 2014

Stress Relaxation Test and Constitutive Equation of Saturated Soft Soil

Πανεπιστήµιο Μακεδονίας Οικονοµικών και Κοινωνικών Επιστηµών Τµήµα Εφαρµοσµένης Πληροφορικής

Cytogenetics and RAPD Ana lysis of In terspec if ic Hybr ids from the Cross of F raga ria m andschu rica Staudt and F. ananassa D uch.

ΑΚΑ ΗΜΙΑ ΕΜΠΟΡΙΚΟΥ ΝΑΥΤΙΚΟΥ ΜΑΚΕ ΟΝΙΑΣ ΣΧΟΛΗ ΜΗΧΑΝΙΚΩΝ ΠΤΥΧΙΑΚΗ ΕΡΓΑΣΙΑ

M in ing Recursive Function s Ba sed on Gene Expression Programm ing

Μελέτη της έκφρασης του ογκοκατασταλτικού γονιδίου Cyld στον καρκίνο του μαστού

Comparison of Evapotranspiration between Indigenous Vegetation and Invading Vegetation in a Bog

Studies on nuclear phase and genetic attribute of the basidiospores of Flammulina velutipes

1 (forward modeling) 2 (data-driven modeling) e- Quest EnergyPlus DeST 1.1. {X t } ARMA. S.Sp. Pappas [4]

C lon ing of 62phosphoglucona te D ehydrogena se Gene ( 6PGD H ) and M olecu2 lar Conf irma tion of A llotetraplo id in C ucum is

ΜΕΛΕΤΗ ΑΠΟΤΙΜΗΣΗΣ ΠΑΡΑΓΟΜΕΝΗΣ ΕΝΕΡΓΕΙΑΣ Φ/Β ΣΥΣΤΗΜΑΤΩΝ ΕΓΚΑΤΕΣΤΗΜΕΝΩΝ ΣΕ ΕΛΛΑ Α ΚΑΙ ΤΣΕΧΙΑ

Stud ies on Spore Propaga tion of P teris cretica A lbo2linea ta

Correction of chromatic aberration for human eyes with diffractive-refractive hybrid elements

(N , 22 ),

ΠΑΝΕΠΙΣΤΗΜΙΟ ΘΕΣΣΑΛΙΑΣ

ΠΑΡΑΜΕΤΡΟΙ ΕΠΗΡΕΑΣΜΟΥ ΤΗΣ ΑΝΑΓΝΩΣΗΣ- ΑΠΟΚΩΔΙΚΟΠΟΙΗΣΗΣ ΤΗΣ BRAILLE ΑΠΟ ΑΤΟΜΑ ΜΕ ΤΥΦΛΩΣΗ

Free Radical Initiated Coupling Reaction of Alcohols and. Alkynes: not C-O but C-C Bond Formation. Context. General information 2. Typical procedure 2

ΠΕΡΙΕΧΟΜΕΝΑ. Μάρκετινγκ Αθλητικών Τουριστικών Προορισμών 1

ΤΕΧΝΟΛΟΓΙΚΟ ΕΚΠΑΙΔΕΥΤΙΚΟ ΙΔΡΥΜΑ ΚΡΗΤΗΣ ΣΧΟΛΗ ΔΙΟΙΚΗΣΗΣ ΚΑΙ ΟΙΚΟΝΟΜΙΑΣ (ΣΔΟ) ΤΜΗΜΑ ΛΟΓΙΣΤΙΚΗΣ ΚΑΙ ΧΡΗΜΑΤΟΟΙΚΟΝΟΜΙΚΗΣ

MSM Men who have Sex with Men HIV -

c Key words: cultivation of blood, two-sets blood culture, detection rate of germ Vol. 18 No

E#ects of Drying on Bacterial Activity and Iron Formation in Acid Sulfate Soils

Journal of Ningxia Medical University BRCA2. M2610I 2405 delt stp ± t = P > %

FENXI HUAXUE Chinese Journal of Analytical Chemistry. Savitzky-Golay. n = SG SG. Savitzky-Golay mmol /L 5700.

ΓΕΩΠΟΝΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙO ΑΘΗΝΩΝ ΤΜΗΜΑ ΑΞΙΟΠΟΙΗΣΗΣ ΦΥΣΙΚΩΝ ΠΟΡΩΝ & ΓΕΩΡΓΙΚΗΣ ΜΗΧΑΝΙΚΗΣ

; +302 ; +313; +320,.

þÿ Ç»¹º ³µÃ ± : Ãż²» Ä Â

ER-Tree (Extended R*-Tree)

Φπκαηίσζε: ε επηδεκηνινγηθή δηεξεύλεζε θξνπζκάησλ κε κνξηαθέο ηερληθέο

Electronic Supplementary Information

ΔΙΠΛΩΜΑΣΙΚΗ ΕΡΓΑΙΑ. Το πέραςμα από την Επιχειρηματολογία ςτην Απόδειξη. ςτη Γεωμετρία: Ο Ρόλοσ τησ Οπτικοποίηςησ ΚΑΡΑΣΡΑΟΓΛΟΤ ΑΘΑΝΑΙΑ

Πτυχιακή Εργασία Η ΠΟΙΟΤΗΤΑ ΖΩΗΣ ΤΩΝ ΑΣΘΕΝΩΝ ΜΕ ΣΤΗΘΑΓΧΗ

Ποιοτική Αξιολόγηση ενός Προγράμματος Διδασκαλίας Δεξιοτήτων Ζωής στη Φυσική Αγωγή

ΓΕΩΠΟΝΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΑΘΗΝΩΝ ΤΜΗΜΑ ΑΓΡΟΤΙΚΗΣ ΟΙΚΟΝΟΜΙΑΣ & ΑΝΑΠΤΥΞΗΣ

1 h, , CaCl 2. pelamis) 58.1%, (Headspace solid -phase microextraction and gas chromatography -mass spectrometry,hs -SPME - Vol. 15 No.

Studies on Cold Tolerance of Rice, Oryza sativa L.

China Academic Journal Electronic Publishing House. All rights reserved. O ct., 2005

Code Breaker. TEACHER s NOTES

8Q5SAC) 8Q5SAC UV2Vis 8500 ( ) ; PHS23C ) ;721 ( ) :1 4. ;8Q5SAC : molπl ;Britton2Robinson Q5SAC BSA Britton2Robinson,

1) Abstract (To be organized as: background, aim, workpackages, expected results) (300 words max) Το όριο λέξεων θα είναι ελαστικό.

Supporting Information

2 PbO 2. Pb 3 O 4 Sn. Ti/SnO 2 -Sb 2 O 4 -CF/PbO x SnO 2 -Sb PbO 2. Sn-Sb 1:1. 1 h. Sn:Sb=10:1. PbO 2 - CeO 2 PbO 2. [8] SnO 2 +Sb 2 O 4 _

Rh(III)-Catalyzed C-H Amidation with N-hydroxycarbamates: A. new Entry to N-Carbamate Protected Arylamines

Reaction of a Platinum Electrode for the Measurement of Redox Potential of Paddy Soil

Supporting Information

ΔΗΜΟΚΡΙΤΕΙΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΘΡΑΚΗΣ ΣΧΟΛΗ ΕΠΙΣΤΗΜΩΝ ΑΓΩΓΗΣ

Δθαξκνζκέλα καζεκαηηθά δίθηπα: ε πεξίπησζε ηνπ ζπζηεκηθνύ θηλδύλνπ ζε κηθξνεπίπεδν.

Μέτρηση της Ρυθµικής Ικανότητας σε Μαθητές Γυµνασίου που Ασχολούνται µε Αθλητικές ραστηριότητες Συνοδευµένες ή Όχι από Μουσική

GPS, 0. 5 kg ( In tegrated Fertility Index, IF I) 1. 1 SPSS 10. IF I =

«ΑΓΡΟΤΟΥΡΙΣΜΟΣ ΚΑΙ ΤΟΠΙΚΗ ΑΝΑΠΤΥΞΗ: Ο ΡΟΛΟΣ ΤΩΝ ΝΕΩΝ ΤΕΧΝΟΛΟΓΙΩΝ ΣΤΗΝ ΠΡΟΩΘΗΣΗ ΤΩΝ ΓΥΝΑΙΚΕΙΩΝ ΣΥΝΕΤΑΙΡΙΣΜΩΝ»

Development of a Seismic Data Analysis System for a Short-term Training for Researchers from Developing Countries

Table S1 RHSW and PHSW from various productive area. RHSW productive area PHSW productive area

ΠΣΤΥΙΑΚΗ ΔΡΓΑΙΑ. Μειέηε Υξόλνπ Απνζηείξσζεο Κνλζέξβαο κε Τπνινγηζηηθή Ρεπζηνδπλακηθή. Αζαλαζηάδνπ Βαξβάξα

«ΙΕΡΕΥΝΗΣΗ ΤΩΝ ΠΑΡΑΓΟΝΤΩΝ ΠΟΥ ΕΠΙ ΡΟΥΝ ΣΤΗΝ ΑΦΟΣΙΩΣΗ ΤΟΥ ΠΕΛΑΤΗ ΣΕ ΕΠΩΝΥΜΑ ΠΡΟΪΟΝΤΑ ΤΡΟΦΙΜΩΝ. Η ΠΕΡΙΠΤΩΣΗ ΤΩΝ ΕΠΩΝΥΜΩΝ ΓΑΛΑΚΤΟΚΟΜΙΚΩΝ ΠΡΟΪΟΝΤΩΝ»

1(a) moat. moat. moat. 1(b) moving magnetic feature

Study on the Strengthen Method of Masonry Structure by Steel Truss for Collapse Prevention

ΙΠΛΩΜΑΤΙΚΗ ΕΡΓΑΣΙΑ. ΘΕΜΑ: «ιερεύνηση της σχέσης µεταξύ φωνηµικής επίγνωσης και ορθογραφικής δεξιότητας σε παιδιά προσχολικής ηλικίας»

DNA2ITS, Iden tif ica tion of C o lle to trichum sp. from A n thu rium and raeanum and Its Sequenc ing of R ibosoma l D NA2ITS

Transcript:

2007, 34 (2) : 305-310 Acta Horticulturae Sinica SSR 1, 1, 1, 2, 1, 1, 23 ( 1, 125100; 2, 100093) : SSR 41, 12 SSR 114, 915 011517 017079, 014630 (BGT23b CHO2D11) 41 2, ; ;,,, : ; ; ; SSR : S 66112 : A : 05132353X (2007) 0220305206 SSR Ana lysis of Genetic D iversity of Pear Cultivars CAO Yu2fen 1, L IU Feng2zhi 1, GAO Yuan 1, J IANG L i2jie 2, WANG Kun 1, MA Zhi2yong 1, and ZHANG Kai2chun 23 ( 1 Research Institute of Pom ology, Chinese A cadem y of A gricultural Sciences, X ingcheng, L iaoning 125100, China; 2 Institute of Forestry and Pom ology, B eijing A cadem y of A griculture and Forestry Sciences, B eijing 100093, China) Abstract: Genetic diversity of 41 pear cultivars was analyzed by using SSR markers. Twelve SSR markers amp lified 114 alleles, w ith an average of 915 alleles per locus. Locus heterozygosity was 011517-017079, and the genetic diversity index was 014630. Two pairs of p rimer (BGT23b and CHO2D11) could distinguish all of the pear cultivars excep t the mutant cultivars. UPGMA cluster analysis showed that the cultivars could be classified into two group s, the occidental pear and oriental pear. Py rus bretschneideri Rehd., P. ussu riensis M axim., and some P. py rifolia ( B urm. f. ) Nakai cultivars originated in China interveined each other; Kuerlexiang and other P. sinkiangensis Y varieties could be clustered together. Chi2 nese P. py rifolia Baozhu and Deshengxiang, Japanese P. py rifolia Shinseiki and Housui, Korean P. py rifolia W hangkeum bae were clustered together in the dendrogram. The coefficient between Q iubai and M ili was high, and they belonged to the same population, so as Nanguo, M anyuanxiang, Q iuzi and B alixiang. J inhua origina2 ted correlatively w ith J inchuanxue and Cangxixue. Key words: Pear; Cultivar; Genetic diversity; SSR ( Pyrus L. ) (Bailey, : 2006-07 - 11; : 2006-12 - 04 3 Author for correspondence ( E2mail: zhangkaichun@baafs1net1cn) 1917), ( P. bretschneideri Rehd. ) [ P. pyrifolia (Burm. f. ) Nakai] ( P. ussuriensismaxim. ) ( P. comm unis L. ) ( P. sinkiangensis Y ), ( Yamamoto et al., 2001, 2002a, 2002b) SSR SSR,

306 34 1 41, ( 1) 1 Table 1 Cultivars of pear for SSR ana lysis ( ) Species (parentage) Code Cultivar O rigin ( ) Species (parentage) Code Cultivar O rigin 1 22 P. bretschneideri Dangshan Suli Dangshan, Anhui Dananguo L iaoning Rehd. 2 23 Jinchuanxue Jinchuan, Sichuan P. pyrifolia Baozhu Chenggong, Yunnan 3 (Burm. f. ) 24 Jinli W anrong, Shanxi Nakai Deshengxiang Sichuan 4 25 Lanzhou Dongguo Longzhong, Gansu Cangxixue Cangxi, Sichuan 5 26 M ili Hebei Shinseiki Japan 6 27 Pingguoli Yanbian, Jilin Housui Japan 7 28 Q iubai Hebei Whangkeumbae Korea 8 29 Xuehua D ingxian, Hebei P. sinkiangensis Kuerlexiang Kuerle, Xinjiang 9 Y 30 Chili Chip ing, Shandong Kunqieke Yecheng, Xinjiang 10 31 Jinfeng Xingcheng, L iaoning Hongnahe Tulufan, Xinjiang 11 32 Jinhua Jinchuan, Sichuan Zaoshujuju Kuche, Xinjiang 12 33 Yali Hebei P. comm unis L. Bartlett B ritain 13 34 Chuizhi Yali Shijiazhuang, Hebei Flem ish BeautyBelgium 14 35 Jinxian Dayali Jinxian, Hebei Clapp Favorite America 15 36 P. ussuriensis Jianba L iaoning Yali Bartlett W ujiuxiang Xingcheng, L iaoning Maxim. 16 37 Huagai L iaoning Nanguo Bartlett Jinxiang Xingcheng, L iaoning 17 38 Manyuanxiang L iaoning Kuerle xiang Xuehua Yuluxiang Taiguo, Shanxi 18 39 Q iuzi Beizhen, L iaoning Pingguoli M ishirazu Zaosu Xingcheng, Liaoning 19 40 Balixiang L iaoning Clappπs Favorite Zaosu Bayuehong Meixian, Shaanxi 20 41 Daxiangshui L iaoning Xuehua Shinseiki Huangguan Shijiazhuang, Hebei 21 Nanguo Anshan, Liaoning

2 : SSR 307 CTAB DNA, 1, DNA SSR ( Gianfranceschi et al., 1998; Yamamoto et al., 2001, 2002a, 2002b) Sangon 22 12 SSR : 20 L 15 20 ng DNA, 10 buffer 210 L, MgCl 2 115 mmol/l, dntp 012 mmol/l, 015 mol/l, Taq 1 U PCR PE PTC100 PCR 94 4 m in; 94 1 m in, 61 58 1 m in, 72 2 m in, 10, 015 ; 94 1 m in, 1 m in, 72 2 m in, 30 ; 72 10 m in; 4 6%, SSR 1, 1, 1, 0 h = 1 - x 2 i, x i i, H = h / n, n NTSYS2pc211, D ice (UPGMA ) 2 211 SSR 12 SSR 41, 114, 3 15, 915 ( 1) F ig. 1 BGT23b 41 1 M: pbr322 DNA /M SP1 markers 1 Am plif ica tion of pr im er BGT23b in 41 pear cultivars The same code as in Table 1. M: 011517 017079, 014630 ( 2) 212 1 DNA,,, SSR, BGT23b 41 15, 30 SSR, BGT23b CHO2D11, 41 12, pbr322 DNA /M SP1 markers. 2 12 SSR 41 Table 2 Amplif ied results of 41 pear cultivars with 12 SSR primers Primer Annealing ( ) Number of alleles detected h Locus heterozygosity KU10 60 55 6 013403 KA4b 60 55 4 011553 KA14 60 55 5 012540 KA16 60 55 14 017079 KB16 60 55 3 011517 BGT23b 60 55 15 015848 NH004a 60 55 12 015140 NH015a 61 56 14 015509 NH009b 58 53 8 015235 NH013a 60 55 8 016401 NH017a 61 56 10 014961 CHO2D11 58 53 15 016371 Average 915 014630

308 34 015882,, ;, 015000 015714 015714,,,,, 015405 015556, 213 12, D ice UPGMA ( 2) 41 0 1100, 0133 0128,, ;,, ( ) ( ), ( ),, 3 F ig. 2 1 2 D endrogram of pear cultivars ba sed on SSR da ta The same code as in Table 1. 12 SSR ( ) ( ), Yamamoto (2001) ( O sa Gold

2 : SSR 309 ) ( ), SSR, SSR (Clapp Favorite) ( ) 12 SSR, D ice,,,,, (1946), ; Teng Tanabe ( 2004), P. pyrifolia var. sinensis (L indley) Y. Teng et K. Tanabe,, 016857, 015455, 015294 015455 015294 015806, 015405 015882;,, 015405 015556,,, (, 1963; Teng et al., 2001), 3, 015000 015714 015946,,, Teng (2001) 3, (, 2001), 415% (, 1983;, 1985;, 1986) NH015a NH015a CHO2D11 3, 12 1 2,,, SSR 20,,, SSR,,, References Bailey L H. 1917. Standard cyclopedia of horticulture. New York: Macm illan: 2865-2878. Chen Rui2yang, L i Xiu2lan, TongDe2yao, L iu Yu2xin, W u Q iu2qin, W ang Feng2shan, Pu Fu2shen, Huang L i2sen. 1983. Study on the chromo2 some number of pear ( Pyrus L. ) in China. Acta Horticulturae Sinica, 10 (1) : 13-15. ( in Chinese) 1,,,,,,,. 1983.., 10 (1) : 13-15. Gianfranceschi L, Seglias N, Tarchini R, Kom janc M, Gessler C. 1998. Simp le sequence repeats for the genetic analysis of app le. Theor. Appl. Genet., 96: 1069-1076. Huang L i2sen, L i Shu2ling, Pu Fu2shen. 1986. Study on the chromosome number of pear cultivars in China. China Fruit, (1) : 12-13. ( in Chinese)

310 34,,. 1986.., (1) : 12-13. Kikuchi A. 1946. A ssessment for Chinese pear species and cultivars. Collected Records for Horticultural Research, 3: 1-11. ( in Japanese). 1946.., 3: 1-11. Pu Fu2shen, Huang L i2sen, Sun B ing2jun, L i Shu2ling. 1985. Study on the chromosome number of wild and cultivated pears ( Pyrus sp. ) in China. Acta Horticulturae Sinica, 12 (3) : 155-158. ( in Chinese),,,. 1985.., 12 (3) : 155-158. Pu Fu2shen, Wang Yu2lin. 1963. China fruit flora, volume (pear). Shanghai: Shanghai Scientific and Technical Press: 304. ( in Chinese),. 1963. g g. : : 304. Teng Y, Tanabe K. 2004. Reconsideration on the origin of cultivated pears native to East A sia. Acta Horticulturae, 634: 175-182. Teng Y, Tanabe K, Tamura F, Itai A. 2001. Genetic relationship s of pear cultivars in Xinjiang, China as measured by RAPD markers. J. Hort. Sci. B iotech., 76: 771-779. Yamamoto T, Kimura T, Sawamura Y, Kotobuki K, Ban Y, Hayashi T, Matsuta N. 2001. SSR s isolated from app le can identify polymorphism and genetic diversity in pear. Theor. App l. Genet., 102: 865-870. Yamamoto T, Kimura T, Sawamura Y, Manabe T, Kotobuki K, Hayashi T, Ban Y, Matsuta N. 2002a. Simp le sequence repeats for genetic anal2 ysis in pear Euphytica, 124: 129-137. Yamamoto T, Kimura T, Shod AaM, Ban Y, Hayashi T, Ban Y, Matsuta N. 2002b. Development ofm icrosatellite markers in the Japanese pear ( P. pyrifolia Nakai). Molecular Ecology Notes, 2: 14-16. Yang Ji. 2001. The formation and evolution of polyploidy genomes in plants. Acta Phytotaxonom ica Sinica, 39 (4) : 357-371. ( in Chinese). 2001.., 39 (4) : 357-371. News CNKI ( 2007 4 ) 1.,,, /1982 /04 298 2, /1991 /02 147 3,,,, /1989 /04 114 4 RAPD,,,, /1998 /04 100 5, /1989 /02 99 6 AFLP,,,, /2000 /02 99 7 RAPD,, /1998 /02 96 8,, /1994 /03 89 9,,,, /1996 /02 83 10 RAPD,,,, /1998 /02 81 11 RAPD,, /1999 /04 79 12 RAPD,,, /1998 /02 79 13 ABA,,,, /1998 /01 78 14,,,, /1998 /04 78 15,, /1987 /02 76 16, /1990 /01 74 17, /1998 /03 73 18,,, /1998 /04 72 19,, /1994 /01 72 20,,,, /1997 /03 70 ( 402 )