49 (5) :693 697, 2003 A cta Zoologica S inica 3 33 (, 100080) (, 610081) (, 201300) Monitoring the menstrual cycle and pregnancy in the Sichuan golden monkey ( Rhinopithecus roxellana) by measuring urinary estradiol and progesterone 3 YAN Cai2E J IAN G Zhi2Gang 33 L I Chun2Wang ZEN G Yan TAN Ni2Ni XIA Shu2Zhong ( Instit ute of Zoology, Chi nese Academy of Sciences, Beiji ng 100080, Chi na) ( Chendu Zoo, Chengdu 610081, Chi na) ( S hanghai Wil d A ni mal Park, S hanghai 201300, Chi na) Abstract During two consecutive mating seasons from August 2001 to December 2002, we observed the sexual behavior of Sichuan golden monkeys in the Chengdu Zoo and the Shanghai Wild Animal Park. We collected urine samples from fe2 males in the early morning. We also measured urinary estradiol and progesterone levels using radioimmunoassay. Based on the estradiol concentration during the menstrual cycle, we discovered that the length of the menstrual cycle was 286133 1167 days. The length of pregnancy was about 200 days. The level of estradiol after impregnation was significantly different with the level during the same period in the non2receptive part of the cycle. Thus we could diagnose early pregnancy in female Sichuan golden monkeys by checking their urinary estradiol level [ Acta Zoologica Sinica 49 (5) : 693-697, 2003 ]. Key words Sichuan golden monkey ( Rhinopithecus roxellana), Estradiol, Progesterone, Radioimmunoassay, Menstrual cycle, Pregnancy 2001),,,, (Li et al., 2001 ; Wasser et al., 1988),, ( Zhang et al., 2000 ;, (Lasley et al.,, 2002) 1991 ; J urke et al., 2000),, ( Whitten et (Ren et al., 1995) (1995) al., 1998 ; Carosi et al., 1999 ; Bardi et al.,, 9 12, 2003201227, 2003204228 3 (No. KSCX221203, No. KSCX22SW2118) [ This research was funded by Knowledge Innovation Pro2 gram of the Chinese Academy of Sciences (No. KSCX221203, No. KSCX22SW2118) ] 33 (Corresponding author). E2mail : jiangzg @panda. ioz. ac. cn, 25, : E2mail : yance @panda. ioz. ac. cn ν 2003 Acta Zoologica Sinica
94 6 49 113, Abraham (1975) Yalow (1992),,,,,,, SN2682, 1 111 2001 8 2002 4, 1, 1 2 ( 1 ) 1,, 610 m 510 m 515 m,, 2001 10 2001 12 2002 9 211 2002 11, 2001 10 12, 14 3, 1 3 1 (2002, 4 ) 3 ; 1 1, (323 223 1 pg/ ml ) 0-2 900 m 2, 5 m 20 m, 0,, + 2 413 m 3 (31 22 pg/ ml ), 1,,, 112,, 1, 7 : 00 8 : 00,,,, 2 ml,, - 20 C,, 1 2 180 Fig. 1 Changes in estradiol concentration during 1 non2conceptive cycles in the Sichuan golden, 46, 2, 3 1 2001 10 12, 3 6 monkey ( Rhinopithecus roxellana)
5 : 695 4 (1 # 2 # 3 # 4 # ), 28133 1167, # 9 10, 4 # 9 18 2 # 10 22 (012 ng/ ml ) 212, 1995), 200, 2 : 1 # 2003 4 10, 3 # 3 29,, 4 # 4 6 : 1 # 2003 0, + 14, 4 12, 3 # 3 31, 4 # 4 10, (52 47 pg/ ml ),, 202 202 204 (185 65 pg/ ml ) (One2Way ANOVA, F 1,8 = 51489, P < 0105), + 40 0 70, 311 ( He et 212 al., 2001), 3 28133 1167,, 200, (193 204),, 2316 315, 204 ( He et al.,,, 2001),, He et al. (2001) 2001 9 6 2002 3 18, 193 214 : 1 # 9 22, 2 # 9 17, 3 ( Zhang et al., 2000 ; 3,, ( Hodges et al., 1986 ;, 2002 Nadler et al., 1993), 2 Fig. 2 Concentration of urinary estradiol before and after impregnation in the Sichuan golden monkey ( Rhinopithecus roxellana) ( The arrow indicates the estimated impregnate date)
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