Chinese Journal of Ecology 2011 30 12 2803-2808 * 1 2 2 3** 2 2 1 225009 2 210008 3 264003 / GC-MS 3 5-7 7 0. 1 g L -1 0. 1 g L -1 GC-MS Q946. 8 A 1000-4890 2011 12-2803-06 Identification of peanut root exudates and their allelopathic effects. WANG Xiao-bing 1 2 LUO Yong-ming 2 3** LIU Wu-xing 2 LI Zhen-gao 2 1 College of Environmental Sciences and Engineering Yangzhou University Yangzhou 225009 Jiangsu China 2 Key Laboratory of Soil Environment and Pollution Remediation of Chinese Academy of Sciences Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 China 3 Yantai Institute of Coastal Zone Research Chinese Academy of Sciences Ynatai 264003 Shandong China. Chinese Journal of Ecology 2011 30 12 2803-2808. Abstract The root exudates of peanut collected by a modified continuous collecting device and XAD-4 ion exchange resin were identified by GC-MS and their allelophatic effects on Ralstonia solanacearum were studied. The root exudates mainly contained acetophenone glycerol benzoic acid 3 5-dimethyl benzaldehyde stearic acid palmitic acid and lactic acid among which only acetophenone had obvious promotion effect on the growth of R. solanacearum at concentration <0. 1 g L -1 and significant inhibitory effect at concentration >0. 1 g L -1. These findings could provide a credible basis for using acetophenone to control the occurrence of R. solanacearum. Key words peanut root exudates GC-MS Ralstonia solanacearum allelopathy. 2009 2011 2005 Sorensen et al. 2005 2006 2007 2010 Kong * 40432005 KSCX2-YW-G-053-3 Gil et al. ** E-mail ymluo@ issas. ac. cn 2011-05-14 2011-09-02 et al. 2008 Li et al. 2010 Senarathne et al. 2010 2011
2804 30 12 Perez & Oremeno-Nunez 1991 1. 5 75% 1 CRETS min 3 ~ 5 0. 1% HgCl Ralstonia solanacearum 5 min 3 ~ 5 25 2 d 1 1. 1 10 d 3 ~ 4 5 ph 5. 8 2. 2 ms cm -1 5 ml min -1 1. 2 12 Teflon 10 cm 15 15 cm BT100-1L 45 Toflen 10 ml 20 mm 250 mm 30 ml 0. 45 μm 0. 5 ~ 1. 0 mm -20 5 mm 1 1 1. 3 1. 6 GC-MS 2 ml L -1 GC- HCl 24 h 10% HCl 1 MS ph 2 ml CH 2 Cl 2 1 ml 250 μl 170 2 h Teflon 121 20 min 1 /10 Hoagland 16 h 25 8 h 16 60% 5 d BSTFA Sigma 75 ~ 80 1 h 1. 4 / GC-MS Agilent 7890GC- 5975MSD CTC 2010 XAD-4 80 1 min 30 min -1 200 6 min -1 250 5 min 30 min -1 270 5 min 260 Hao et al. 2007 XAD-4 GC MS 280 He Sigma 50 1 1. 2 ml min -1 3 1. 0 μl 24 h 2009 1. 7 20 mm 200 mm 190 mm 175 ml 10 ~ 15 0. 375 g 15
2805 g L -1 ph 7. 0 0. 15 1. 5 3. 0 7. 5 15 g L -1 1 ml 30 ml 0 0. 005 0. 05 0. 1 0. 25 0. 5 g L -1 YGPA 3 d 2 ml 10 μl 200 r min -1 30 18 h 4 5 g L -1 0. 5 g L -1 1 1. 5 ph 7. 0 121 20 min YGPA 10 g 5 g 5 g ph 7. 2 2 2. 1 1 Fig. 1 Cycling collection device of peanut root exudates Shen et al. 2001 Hoffland et al. 2. 2 1989 C 14 McDougall & Rovira 1970 Shen et al. 2005 10 d 3 ~ 4 5 d Tang & Young 1982 microdialysis 2005 / GC-MS2 2 CRETS Tang 15. 3 min Young 1982 3 m /z 149 167 279 1995 2003 2010 1 1 Teflon BSTFA m /z Teflon 73 C n H 2n+1 2 m /z 77 66 65 51 40 39 m /z 91 105 1
2806 30 12 2 Fig. 2 GAS-chromatogram of peanut root exudates 3 Fig. 3 GAS-chromatogram of CK 1 Table 1 Identification of peanut root exudates min % 1 3. 596 10. 15 2 3. 711 2. 95 3 4. 421 10. 15 3 5-4 4. 495 2. 52 5 4. 579 3. 57 6 9. 457 9. 81 7 11. 500 9. 42 2. 3 YGPA C 18 h 2 2. 4 18 h 3 3 0. 1 g L -1 0. 1 g L -1 P<0. 05 0. 1 g L -1 P < 0. 05 3 Uselman 2000 60%
2807 2 Table 2 Absorbance of peanut root exudates on Ralstonia solanacearum 3 5- OD 600 0. 030 0. 021 0. 012 0. 013 0. 024 0. 015 0. 012 0. 068 0. 1 g L -1 Hyder et al. 2002 4 Fig. 4 Effect of acetophenone on Ralstonia solanacearum Tang & Young 1982 Hao et al. 2007. 2003.. 25 6 5-10.. 2005.. 20 3 330-337.. 2003. XAD-4. 35 4 311-313. GC-MS. 2009.. 7 11 4 107-111.. 2009.. 21 8 38-39. 7 GC-MS. 2007.. 2 32-36.. 2005.. 34 2 17-22. NMR. 2011.. 48 4 53-58.. 2010. 2009. 39 2 6-10.. 2010.. 47 4 747-752.. 2006.. 35 1 29-33.. 2010..
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