838 Acta Pharmaceutica Sinica 2003 38(11) :838-842 3 ( 430070) : ; M w S 2 1Π2 A 2 1104 10 6 (10510 019) nm ( - 1159 0128) 10-3 mol ml g - 2 M w ΠM n 1102 Mark2Houwink [] = 5196 10-2 M 0173 w ; M L = 982182 nm - 1 q = 27193 nm d = 0174 nm h = 0126 nm L = 1 054111 nm : ; ; ; ; : R912 :A : 0513-4870(2003) 11-0838 - 05 Study on molecular chain morphology and chain parameters of konjac glucomannan LI Bin XIE Bi2jun 3 ( Natural Product Chemistry Research Laboratory Food Science Department of Huazhong Agricultural University Wuhan 430070 China) Abstract : Aim To study the molecular chain morphology and chain parameters of konjac glucomannan ( KGM). Methods Solution behavior was studied by using light scattering(ls) gel permeation chromatograph (GPC) and method of viscosity. The molecular morphology was observed directly by atom force microscope (AFM) and transmission electron microscope ( TEM). Results The average molecular weight ( M w ) root2 mean2square ratio of gyration (S 2 1Π2 ) second Viral coefficients ( A 2 ) and multi2disperse coefficients ( M w Π M n ) are 1104 10 6 10510 019 nm ( - 1159 0128) 10-3 mol ml g - 2 and ( 11020 01003) respectively ; Mark2Houwink equation was established as [] = 5196 10-2 M 0173 w the molecular chain parameters were as follows : M L = 982182 nm - 1 q = 27193 nm d = 0174 nm h = 0126 nm L = 1 054111 nm. Conclusion Both the result showed by direct observation and the deduction drawn by solution behavior confirmed that the KGM molecular is stentering semi2flexible linear chain without branch. Key words : konjac glucomannan ; molecular chain parameter ; molecular morphology ; atom force microscope ; light scattering [14 ] : 2003201218. : (2001AA207B01) 3 Tel : 86-27 - 87283201 E2mail : zpp @public3. bta. net. cn [5 ]
510 g L - 1 Waters GPC 2000 Sephadex G2100 20 L 012 mol L - 1 NaCl ( konjac glucomannan KGM) 110 L min - 1 20 min 2D2 ( Glc) 2D2 (Man) 1 116 [6 ] Pullulans 1 1169 [7 ] Maeda [6 ] : DAWN2DSP KGM 21 42 (Wyatt Technology Co. USA) P100 [810 ] C 3 TSK2gel G7000 HHR (718 mm 300 mm) 21 32 32 150 (DRM21020 3 19 1 Otsuka Electronics Co. ) 633 nm 25 [6 Maeda ] KGM 1112 10 6 21619 10 5 Xu [8 ] 48 h KGM 8109 10 5 KGM 012m (M2HJV) KGM 012 mol L - 1 NaCl (d n Πd c ) 0114 ml g - 1 KGM Astra 50 % 50 ml 3 Π (2 1) 50 ml 40 8 h 016 % 115 10-3 g L - 1 TM ( Himac Centrifuge (6m 6m) (20m 20m) ( Hitachi) 16 000 r min - 1 20 min ) XY < 014 nm Z < 011 nm 20 mm 011 m Tapping ; 34 nn Sevag 5 (20 ) 40 % 60 %80 % 30 % (Alphal2Z Germany) KGM 1 KGM KGM KGM M n 1104 10 6 M p 1103 10 6 M w 1109 10 6 M w ΠM n = 1104 4 KGM ( KGM 1 KGM 2 KGM 3 KGM 4 ) KGM 2 : 839 : RI2 (0158 mm) 25 ( 0105 ) 012 mol L - 1 NaCl ( Amorphophallus konjac K. KGM : KGM Koch) 1 % 1510 Pa s (40 ku) 3 5 - (DNS ) (60 ku) Sigma 115 10-4 g L - 1 KGM 1010 g 7 ( Philips Tem400st 011 % ( ) Holand) 40 000 : AJ2III SPM
840 Acta Pharmaceutica Sinica 2003 38(11) :838-842 M w ΠM n = 1102 M n M w M z 1103 10 6 1104 10 6 1104 10 6 S 2 10510 nm 2 2. 2 KGM ( [14 ) KGM KGM ( MwΠ[]) 2 1Π3 = I + SM 1Π2 w I = 11541 6 10-8 A 0 M L (g 1Π3 cm - 1 ) KGM 012 mol L NaCl Zimm (1) KGM M w 1103 10 6 S 2 1Π2 10410 nm M L nm - 1 A 2 ( - 1159 0128) 10-3 mol ml g 2 L p A 2 B 0 11051108 KGM B 0 = 11065 KGM S 2 1Π2 KGM KGM 1 KGM 4 M w [] [11 ] ( MwΠ[]) 2 1Π3 M 1Π2 [12 ] w I S I = 201151 S = 0168 L2F v [13 ] KGM d 2 rπa 0 = (4 0 Π11215N A ) (ΠI) S 4 Figure 1 Zimm plot of konjac glucomannan ( KGM) in 012 mol L - 1 NaCl 2 KGM 2. 1 x KGM KGM 1 2KGM 4 M w [] log[]logm w X R 2 01987 : KGM M L = 982182 nm - 1 L p = 27193 nm d = 0174 nm K = 5196 10-2 = 0173 h = 0126 nm L = 1 054111 nm X = 4 054127 KGM Mark2Houwink [] = 5196 10-2 M L ( ) M w 0173 = 0173 KGM S = 11541 6 10-8 B 0 (2L p ΠM L ) - 1Π2 (g 1Π3 cm - 1 ) log( d 2 rπa 0 ) = 01173 + 21158 log d r 0 Flory d r 011 2186 10 23 N A Avogadro ( ) nm - 1 KGM M L 982182 nm - 1 2D2 1 030 nm - 1 (1 940 nm - 1 ) (2 170 nm - 1 ) d 2 rπa 0 = 1123 10-4 d r = 13115 10-3 A 0 = 1133 d = d r 2L p h = ( M 0 Πx)ΠM L L = M w ΠM L X = LΠd d nm h ( ) nm M 0 M 0 = 162 L nm 500 2200
520 nm - 1 KGM L p L p : (17 nm) < KGM (27193 nm) < 2D2 (90 nm) < (120 nm) < (200 nm) KGM L p 3 : 841 TEM Figure 2 TEM image of KGM AFM 10 [15 ] 315 % KGM KGM ; 34 nn M L = KGM 982182 nm - 1 L 9501 100 nm 1 020 nm p = 27193 nm d = 0174 nm h = 0126 017110 nm nm L = 1 054111 nm 315 % AFM KGM 3510 nm References : ( [1 ] Yan BX Li YL Su YF et al. Action of glucomannan in amorphophallus rivieri durieu on mutured obese rats [ J ]. 0111 nm) Northwest Pharm J ( ) 1998 13 (4) :32-35. [ 2 ] Gu YD Shi JX Hu ZY. Fight against senium action of konjac polysaccharides [J ]. Chin Tradit Herb Drugs ( Bustamanter [16 ] ) 1999 30 (2) :78-81. DNA [ 3 ] Mao CP Yu NY Gu ZL. KGM 40 (2) Hypoglycemic effects of amorphophallus konjac in alloxan diabetic rats [J ]. Chin J KGM Mod Appl Pharm ( ) 2001 18 (3) :12 Xu [8 ] - 17. Ogawa [17 ] [ 4 ] Liu H. Therapeutic effect of glucomanan on experimental diabetic mice [J ]. ( ) ) 2002 18 (1) :12-17. 9501 125 nm 1 050 [5 ] Chong ZZ. Konjac Sience ( ) [ M ]. Chongqin : nm AFM AFM Chongqin Food Industry Institute 1987. 95-98. [6 ] Maeda M Shimahara H Sugiyama N. Detailed examination ( ) of the braced structure of konjac glucomannan [ J ]. Agric 015110 Biol Chem 1980 44 (2) :245-252. nm( 017 nm) [7 ] Jia CY Chen SW Mo WP. Study on amorphophallus albus Chin Pharmacol Bull (
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