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620 ( ) 29. Lau [27 ] 16 rdna, ITS2 12. 4 %, 29. 8 % 18. 8 %, ITS2.,, [28 ] rdna ITS, 28,. Cao [29 ] 6 18S rrna t rn K, 18S rrna 1 810 bp, 1, t rn K,, t rn K 6. [30 ] PCR ITS1, ITS1 248 bp, 7, 12,. [31 ] 18S rrna,, 2. [32 ] 4 18S rrna m at K,. Tomari [33 ] rps rp116 rp114, 2 DNA. Kojoma [34 ], t rnl - t rn F t rnl 1 3 4,. [35 ] 2 2 5 rbcl,,, 5. [36 ] 3 4 rdna ITS, ITS1, ITS2 2. 10 % 2. 09 %, ITS1, ITS2 8. 14 % 12. 82 %, ITS., [37 ] 10 12 rdna ITS, 2,2 15, 2. [38 ] ( ) rdna ITS1 5. 8S rrna 18S rrna,. [39 ] 16 rdna,,, ITS, ITS1 ITS1 ITS2, ITS,. Yang [40 ] 13 26 49 t rnl t rn F,,,,. Tsoi [41 ] 9 26S rdna D2 D3, DNA,, DNA. 3,.,,. DNA,,. DNA,. DNA, ;.,,, ;,,., DNA,.

6 : DNA 621 : [1 ],. [J ].,2000,18 (5) :2602262. [2 ] BOSTEIN D R,WHITE R L,SKOLN ICK M, et al. Construction of a geneticlinkage map in man using restriction frag2 ment length polymorphism [J ]. Am J Hum Genet,1980,32 :3142331. [ 3 ] WILL IAMS J G K, KUBEL IK A R,L IVSK K L, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers [J ]. Nucleic Acids Research,1990,18 (22) :6 53126 535. [4 ] VOS P. AFL P :A new technique for DNA fingerprinting [J ]. Nucl Acids Res,1995,23 :4 40724 414. [5 ],,,. DNA [J ].,2002,9 (5) :3152320. [6 ],,,. PCR [J ].,1999,30 (8) :6282630. [7 ],,,. PCR RFL P ( ) [J ]., 1995,37 (2) :972102. [ 8 ] MIZU KAMI H,SHIMIZU R, KOHDA H, et al. Restriction fragment length polymorphism of rdna and variation of es2 sential oil composition in astractylodes [J ]. Plants Biol Pull,1996,19 (4) :5772580. [9 ] N GAN F N, SHAW P C,BU T P P H, et al. Molecular authentication of Panax species [J ]. Phytochemistry Oxford, 1999,50 (5) :7872791. [10 ],,,. AFL P [J ].,2000,25 (12) :7072710. [11 ],,,. AFL P DNA [J ].,2000,35 (8) :6262629. [12 ],,,. 4 AFL P [J ].,2004,35 (7) :8082810. [13 ] NA KAI R,SHO YAMA Y,SHIRASHI S. Genetic characterization of epimedium spacies using random amplified poly2 morphic DNA ( RAPD) and PCR 2 restriction fragment length polymorphism ( RFL P) diagnosis [J ]. Biol Pharm Bull, 1996,19 (1) :67270. [14 ],,,. RAPD 7 [J ].,1996,27 (11) :6862687. [15 ],,,. RAPD [J ].,2000,31 (9) :6972700. [16 ] ZHAN G K Y B,L EUN G H W, YEUN G H W, et al. Differentiation of L ycium barbarum from its related L ycium species using random amplified polymorphic DNA [J ]. Planta Med,2001,67 :3792381. [17 ] LA Y H L,L IU H J,L IAO M H, et al. Genetic identification of Chinese drug materials in Yams by RAPD analysis [J ]. Food Drug Anal,2001,9 (3) :1322138. [18 ],,. RAPD [J ].,2001,26 (1) :8210. [19 ],,,. [J ]. ( ),2002,41 (6) :6642668. [20 ],,,. [J ].,2002,33 (12) :1 1132 1 116. [21 ],,,. RAPD [J ].,2003,5 (2) :1562157. [22 ],,. RAPD [J ].,2003,34 (6) :5572560. [23 ],,. RAPD [J ].,2004,27 (7) :4692471. [24 ],,. DNA [J ].,2004,27 (3) :1642169. [25 ],,,. RAPD [J ].,2003,26 (12) :8852886. [26 ],,. nrdna ITS [J ].,2000,9 (3) :38240. [27 ] LAU D T W,SHAW P C,WAN G J, et al. Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA [J ]. Planta Med,2001,67 :4562460. [28 ],,. nrdna ITS [J ].,2001,29 (3) :43246. [ 29 ] CAO H,SASA KI Y,FUSHIMI H, et al. Molecular analysis of medicinally used Chinese and Japanese Curcuma based on 18S rrna gene and t rn K gene sequences [J ]. Biol Pharm Bull,2001,24 (12) :1 38921 394. [30 ],,. ITS1 [J ].,2001,24 (7) :4812483. [31 ],,. ( ) DNA

622 ( ) 29 [J ].,2001,21 (6) :4232427. [32 ],,,. DNA [J ].,2001,24 (6) :3982402. [33 ] TOMARI N,ISHIZU KA I,MORIYA A, et al. Phylogenetic relationship of the Cistanche plants based on plastid rps2 gene and rp116 rp114 intengenic spacer sequences [J ]. Biol Pharm Bull,2002,68 :2182222. [ 34 ] KOJOMA M, KURIHARA K, YAMADA K, et al. Genetic identification of Cinnamon based on the t rnl t rnf chloro2 plast DNA [J ]. Planta Med,2002,68 :94296. [35 ],,,. rbcl [J ].,2003,38 (2) :1472152. [36 ],,. rdna ITS [J ].,2003,38 (3) :1732175. [37 ],,. ITS [J ].,2003,28 (8) :7062710. [38 ],,. rdna ITS [J ]. ( ),2004,27 (2) :1882191. [39 ],,,. rdna ITS [J ].,2004,35 (5) :5592562. [ 40 ] YAN G M H,ZHAN G D M,L IYJ Q, et al. A molecular marker that is specific to medicinal rhubarb based on chloroplast t rnl/ t rnf sequences [J ]. Planta Med,2001,67 (8) :7842786. [41 ] TSOI P Y,WOO H S,WON G M S, et al. Genotyping and species identification of Fritillaria by DNA chips [J ]. Acta Pharmaceutica Sinica,2003,38 (3) :1852190. Application of D NA Molecular Marking Technology in Identif ying Chinese Herbal Medicine L I Wen2qiang 1, HUAN G Shi2liang 1, N IU Yu2lu 1,2, ZHAO Jian2cheng 1, WAN G Xiao2rui 1 (1. College of Life Science,Hebei Normal Universit y,hebei Shijiazhuang 050016,China ; 2. Department of Biology,Hengshui College,Hebei Hengshui 053000,China) Abstract :DNA molecular marking technology wit h st rong specificity,reliability and sensitivit y lead to great progress in identification of Chinese herbal medicine. The techniques including RFL P, RAPD,AFL P and DNA sequencing have been extensively studied and great achievements have been acquired in the field. The principles and characteristics and t he most advances in application of t hese techniques in recent years are discussed. Furthermore,great future of these techniques is discussed. Key words : DNA molecular marking ; Chinese herbal medicine ; identification ; DNA finger2printing technique ; DNA sequencing ( )