Supplementary Materials for

www.sciencemag.org/content/350/6266/1383/suppl/dc1 Supplementary Materials for RNA polymerase II associated factor 1 regulates the release and phosphorylation of paused RNA polymerase II Ming Yu, Wenjing Yang, Ting Ni, Zhanyun Tang, Tomoyoshi Nakadai, Jun Zhu, Robert G. Roeder* *Corresponding author. E-mail: roeder@rockefeller.edu Published 11 December 2015, Science 350, 1383 (2015) DOI: 10.1126/science.aad2338 This PDF file includes: Materials and Methods Supplementary Text Figs. S1 to S11 Tables S1 to S5 References

Materials and Methods Cell culture and treating cells with chemicals The human AML THP1 cells and the human ALL CCRF-CEM cells were cultured in RPMI- 1640 + 10% FBS + 2% Penicillin/Streptomycin + 2 mm L-Glutamine + 55 µm β- mercaptoethanol. Flavopiridol (Sigma, cat. no. F3055) treatment was performed as previously described (2). THP1 cells were cultured in standard medium supplemented with DMSO or 1 µm flavopridol in DMSO for 1 hour before fixation for ChIP. Chromatin immunoprecipitation (ChIP) and ChIP-sequencing (ChIP-seq) ChIP assays were performed as previously described with minor modifications (24). Normally, cells were fixed with 0.4% (v/v) formaldehyde at room temperature for 10 min. To improve the ChIP efficiency, double fixation was used. For double fixation with EGS (Thermo, cat. no. 21565) and formaldehyde, cells were fixed initially with 1.5 mm EGS at room temperature for 30 min, and subsequently with 0.4% formaldehyde at room temperature for 10 min. For double fixation with DMA (Thermo, cat. no. 20660) and formaldehyde, cells were fixed initially with 25 mm DMA at room temperature for 1 hour, and subsequently with 0.4% formaldehyde at room temperature for 10 min. For sonication, fixed cells were washed twice with PBS and resuspended in ice-cold RIPA-0.3 buffer (10 mm Tris-HCl, 1 mm EDTA, 1% Triton X-100, 0.1% SDS, 0.1% NaDOC, and 0.3 M NaCl, ph 7.4) supplemented with Protease Inhibitor Cocktail (Millipore, cat. no. 535140) at a concentration of 40 million cells/ml; genomic DNA was disrupted to a size range of 100 to 500 bp. For immunoprecipitation, on day 1 antibodies were diluted in RIPA-0.3 and bound to Dynabeads protein A (Thermo, cat. no. 10002D) by incubating at 4 C for 3 hours. Afterwards, the bead-antibody complexes were washed twice with RIPA-0.3 and then incubated with sonicated chromatin at 4 C overnight. On day 2, after 2 washes with RIPA-0.5, 1 wash with RIPA-0.3, 1 wash with RIPA-0, 2 washes with LiCl buffer (10 mm Tris-HCl, 1 mm EDTA, 0.25 M LiCl, 0.25% NP-40, and 0.25% NaDOC, ph 7.4), and 2 washes with TE buffer, bound protein-dna complexes were resuspended in elution buffer (10 mm Tris-HCl, 1mM EDTA, 0.2 M NaCl, and 1% SDS, ph 7.4) supplemented 2

with 10 µg/ml RNase A for both elution and RNA digestion, and incubated at 55 C for 1 hour. Then Proteinase K was added to a final concentration of 200 µg/ml, and after 30 min incubation the temperature was increased to 65 C for crosslink reversal. After incubation for 4 to 6 hours, DNA was purified by ChIP DNA Clean & Concentrator (Zymo Research, cat. no. D5205). For qpcr, a site 2 kb downstream of the TSS of the NANOG gene was used as an internal control, and fold-enrichment was calculated by the 2 ΔCt method. The antibodies and the corresponding fixation conditions that were used for ChIP assays are listed in table S4. Real-time PCR primers for ChIP are listed in table S5. ChIP-seq libraries were constructed with 2 10 ng immunoprecipitated DNA. After endrepair, A-tailing, and barcode ligation, barcoded DNA was amplified by 16- to 18-cycle PCR. Libraries were sequenced on Illumina Genome Analyzer II, HiSeq 2000, or HiSeq 2500 by following the manufacturer's protocols. After decoding, raw reads were mapped to reference genome (hg19) using BWA through GALAXY. ChIP-seq analysis HOMER (25) was used to generate BedGraph files and for calculating peak genomic distribution. ChIP-enriched regions were identified using SICER (26), and plots of binding-onan-average-gene were generated as previously described (2). RNA interference plko.1 TRC control and mission shrna clones were purchased from Sigma Aldrich. For lentivirus production and transduction, 60-90% confluent 293T cells in antibiotic-free medium were transfected on day 1 with TRC control or gene-specific shrna clones along with packaging plasmids pspax2 and pmd2.g. On day 2 in the morning, medium containing transfection reagent was replaced by fresh medium containing 2% Penicillin-Streptomycin (Sigma Aldrich, cat. no. P0781). On day 3 in the afternoon, THP1 or CCRF-CEM cells were resuspended in virus-containing medium, and spun at 2,000 rpm at 20 C for 1 hour. After spin infection, virus-containing medium was removed and cells were resuspended in fresh medium and cultured overnight. On day 4 in the morning, cells were washed twice with PBS and 3

resuspended in fresh medium. On day 5 in the morning, puromycin was added to a final concentration of 2 µg/ml. Usually, cells were cultured for additional 72 hours before being harvested for qrt-pcr, Western blot, and ChIP. For Western blots and ChIP assays comparing the protein levels or chromatin occupancies of total Pol II and CTD serine-phosphorylated Pol II in control and knockdown cells, cells were cultured for additional 120 hours before being harvested. Mission shrna clones used in this study are listed in table S5. RNA extraction, reverse transcription, real-time PCR, and RNA-seq RNA was extracted from cells using RNeasy Plus Mini Kit (Qiagen, cat. no. 74134) or Quick-RNA MiniPrep Kit (Zymo Research, cat. no. R1054) by following the manufacturer s protocol. For reverse transcription, single-strand cdna was synthesized using iscript cdna Synthesis Kit (Bio-Rad, cat. no. 1708891). For real time PCR, GAPDH was used as the internal control. Relative abundance of transcripts was calculated by the 2 ΔCt method. Primers for real time PCR are listed in table S5. Libraries of strand-specific RNA-seq were constructed as previously described (27). Co-immunoprecipitation (co-ip) and pull-down assay Co-IP experiments were performed as previously described with minor modification (24). On day 1, antibodies were incubated with Dynabeads protein A at 4 C for 3 hours and then crosslinked to beads by 25 mm DMA at room temperature for 1 hour. Nuclear extract (NE) from THP1 cells was diluted 2 to 3 fold by NE dilution buffer (31 mm Tris-HCl, 1.6 mm EDTA, and 0.16 % NP-40 (v/v), ph 7.5); 0.5 to 1 mg NE was used for each co-ip, and incubated with beadantibody complexes at 4 C overnight. On day 2 in the morning, bead-antibody-protein complexes were washed with BC150 buffer (10 mm Tris-HCl, 0.2 mm EDTA, 150 mm KCl, 20% glycerol, and 0.1% NP-40, ph 7.9) for 4 times. Afterwards, protein complexes were eluted from beads by 50 mm glycine (ph 2.4), immediately neutralized in 1 M Tris-HCl, ph 7.4, and analyzed by Western blot. Pol II was purified as previously described (28). For pull-down assays, around 10 pmol tagged bait protein was bound to anti-affinity tag antibody-coated magnetic beads by incubation 4

at 4 C for 2 hours. After 4 washes with BC300 and 2 washes with BC150, purified prey protein was added, and incubated at 4 C for 2 hours. After 4 washes with BC150, the protein complexes were eluted by boiling beads for 2 min in 1 X SDS-PAGE loading buffer and analyzed by Western blot. Antibodies used for Western blot and Co-IP experiments are listed in table S4. P-TEFb release assay P-TEFb release assays were performed as previously described (29). Briefly, LARP7 antibody (Bethyl, A303-724A) was used to immunoprecipitate 7SK snrnp complex. The 7SK snrnp complex was incubated with 4 µg RNase A (Life Technologies, cat. no. AM2270) or increasing amounts of purified PAF1C at 37 C for 15 min. After washing, CDK9 that remained on beads was analyzed by Western blot. 5

Supplementary Text Author Contributions M.Y. and R.G.R. designed the experiments. M.Y. performed the experiments and analyzed the data. W.Y., M.Y., T. Ni, and J.Z. performed the bioinformatics analyses. Z.T. purified PAF1C, and T. Nakadai purified Pol II. M.Y. and R.G.R wrote the paper. 6

Fig. S1. PAF1C occupancy positively correlates with gene expression level. (A) to (C) Metagene analyses showing positive correlation between the occupancies of CDC73 (A), LEO1 (B), and CTR9 (C) and gene mrna levels (High, Medium, Low, Silent). (D) to (F) IGV browser snapshots comparing the occupancy of the PAF1, CDC73, LEO1, and CTR9 subunits of PAF1C, total Pol II, and CTD serine 2-phosphorylated Pol II (ser-2p) within the RUNX1 (D), RUNX2 (E), and MEF2C (F) loci in THP1 cells. 7

Fig. S2. PAF1 knockdown increases promoter-proximal pausing of Pol II in THP1 cells. (A) and (B) IGV browser snapshots comparing the occupancies of PAF1, total Pol II, and CTD serine 5- phosphorylated Pol II (ser-5p) within the c-myc (A) and the CTNNB1 (B) loci in control and PAF1 knockdown THP1 cells. 8

Fig. S3 PAF1C is a critical regulator of promoter-proximal pausing release of Pol II in human cells. (A) Comparison of PAF1 levels in control and PAF1 knockdown CCRF-CEM cells by Western blot. PAF1 shrna #2 was used for the knockdown, and β-actin was used as a loading control. (B) and (C) Comparison of the occupancies of PAF1 (B) and Pol II (C) on an average gene in control and PAF1 knockdown CCRF-CEM cells. (D) and (E) IGV browser snapshots comparing the occupancies of PAF1 and Pol II within the RUNX1 (D) and the BCL11A (E) loci in control and PAF1 knockdown CCRF-CEM cells. (F) and (G) Comparison of the occupancies of PAF1 (A) and Pol II (B) within the c-myc locus in control and PAF1 knockdown CCRF-CEM cells. Error bars indicate mean ± SD (N=3). 9

Fig. S4 Flavopiridol treatment increases promoter-proximal pausing of Pol II but decreases the chromatin occupancy of LEO1 in THP1 cells. (A) and (B) IGV browser snapshots comparing the occupancies of Pol II and LEO1 within the c-myc (A) and the c-myb (B) loci in DMSOand flavopiridol-treated THP1 cells. 10

Fig. S5 Knockdown of the SPT5 subunit of DSIF reduces the occupancy of both Pol II and PAF1. (A) Comparison of the levels of SPT5 in control and SPT5 knockdown THP1 cells. Two shrnas were able to efficiently reduce the level of SPT5. β-actin was used as a loading control. (B) to (D) ChIP-qPCR data comparing the occupancies of SPT5 (B), Pol II (C), and PAF1 (D) within the c-myc locus in control and SPT5 knockdown THP1 cells. Error bars indicate mean ± SD (N=3). 11

Fig. S6 CDK9 knockdown preferentially reduces the CDK9 fraction in 7SK snrnp. (A) Comparison of Pol II occupancies on an average gene in control and CDK9 knockdown THP1 cells. (B) CDK9 knockdown preferentially reduces the CDK9 fraction in 7SK snrnp. 12

Fig. S7 PAF1C does not effect the release of P-TEFb from 7SK snrnp. PAF1C is incapable of extracting P-TEFb from 7SK snrnp in release assays. RNase A was used as a positive control of P-TEFb release. 13

Fig. S8 PAF1 knockdown reduces Pol II CTD serine-2 phosphorylation but increases Pol II CTD serine-5 phosphorylation. (A) Comparison of the levels of PAF1, Cyclin K, CDK9, total Pol II, Pol II (ser-5p), and Pol II (ser-2p) in control, PAF1 knockdown, CDK9 knockdown, and Cyclin K knockdown THP1 cells by Western blot. β-actin was used as a loading control. (B) and (C) ChIP-qPCR data comparing the occupancies of Pol II (ser-5p) (B) and Pol II (ser-2p) (C) within the c-myc and the CTNNB1 loci in control and PAF1 knockdown THP1 cells. Error bars indicate mean ± SD (N=3). 14

Fig. S9 PAF1 knockdown reduces the chromatin occupancy of CDK12 but not that of BRD4. (A) and (B) ChIP-qPCR data comparing of the occupancies of CDK12 (A) and BRD4 (B) within the c-myc locus in control and PAF1 knockdown THP1 cells. Error bars indicate mean ± SD (N=3). 15

Fig. S10 Cyclin K knockdown does not affect the chromatin occupancy of PAF1C. (A) Comparison of Cyclin K levels in control and Cyclin K knockdown THP1 cells by Western blot. (B) to (D) ChIP-qPCR data comparing the occupancies of CDK12 (B), PAF1 (C) and Pol II (D) within the c-myc locus in control and Cyclin K knockdown THP1 cells. Error bars indicate mean ± SD (N=3). 16

Fig. S11 Model for PAF1C function in the release and phosphorylation of promoter-proximal paused Pol II. The model emphasizes (i) the previously established (1) role of DSIF and NELF in the establishment of Pol II (CTD ser-5p) pausing and the role of P-TEFb in paused Pol II release through phosphorylation of NELF (leading to its dissociation), DSIF (leading to its conversion to a positive regulator and the creation of PAF1C binding sites on the SPT5 subunit) and Pol II CTD (ser-2), (ii) the herein established role of PAF1C as a positive regulator of pausing release, with PAF1C recruitment and Pol II association facilitated by direct interaction with promoter-associated P-TEFb, and (iii) the herein established recruitment of CDK12/CycK to the elongating Pol II-PAF1C complex, through a direct PAF1C CDK12/CycK interaction, for consequent downstream Pol II CTD (ser-2) phosphorylation. Not shown are factors implicated in P-TEFb recruitment and PAF1C interactions implicated in histone modifications and transcription-coupled RNA processing events (6). Also, as discussed in the text, recent and current studies have documented both positive and negative functions for PAF1C that are dependent on the genetic background and physiological states of the cells. The asterisks in the figure represent phosphorylation events and the short solid black bars indicate direct interactions that are newly described in the current study. SEC refers to the super elongation complex comprised of P-TEFb (CDK9+CycT), AF9, AFF1/AFF4, and ELL 17

Table S1. Lists of direct target genes of PAF1 in THP1 cells 18

table S1. Direct taget genes of PAF1 Downregulated (FC >2) #GeneName FC(WT/KD) P- value PDR 6- Sep 2.04087268 1.42E- 05 0.00034008 AARS 2.45141433 1.42E- 08 1.16E- 06 ACAT2 2.2692087 3.27E- 07 1.55E- 05 ACOT11 2.0430517 1.72E- 05 0.00040111 ACOT7 2.1605579 1.72E- 06 6.03E- 05 ADCK2 2.25391867 4.32E- 07 1.95E- 05 ADM2 4.52831048 2.22E- 06 7.39E- 05 AES 2.00318551 1.29E- 05 0.00031694 AIG1 2.63994465 1.28E- 09 1.52E- 07 AKR7A2 2.12051254 2.13E- 06 7.14E- 05 ALKBH3 2.05599539 1.31E- 05 0.0003198 ANKLE1 2.61701517 8.25E- 09 7.27E- 07 ARL6IP6 2.86446876 2.27E- 10 3.32E- 08 ARMC7 2.17843364 9.05E- 06 0.00023635 ASF1B 2.11120021 2.76E- 06 8.84E- 05 ATF4 3.81051626 1.09E- 16 9.18E- 14 ATIC 2.18333403 7.75E- 07 3.13E- 05 ATP8B3 2.30371628 3.53E- 07 1.66E- 05 AXL 2.16656077 6.08E- 06 0.00016951 B3GNT7 2.48008906 1.70E- 08 1.33E- 06 B4GALT2 2.80894292 1.67E- 10 2.52E- 08 BAG2 2.08064505 6.49E- 06 0.00017869 BATF 2.121087 5.19E- 06 0.0001488 BATF3 2.83640531 3.44E- 08 2.38E- 06 BDH1 2.65452812 7.85E- 09 7.01E- 07 BEND5 3.56609113 4.50E- 06 0.00013266 BRCA1 2.19989044 6.73E- 07 2.79E- 05 BRIP1 2.53396693 5.97E- 08 3.67E- 06 BTBD2 2.40109185 3.46E- 08 2.38E- 06 C12orf10 2.100941 4.70E- 06 0.00013714 C14orf149 2.86092168 3.66E- 08 2.49E- 06 C14orf159 2.01541929 1.41E- 05 0.00033748 C16orf59 2.69902067 4.03E- 09 4.12E- 07 C16orf93 2.43531615 2.64E- 07 1.29E- 05 C19orf54 2.06626661 1.37E- 05 0.0003294 C19orf70 2.21252472 1.05E- 06 3.97E- 05 C1orf200 3.01461117 1.08E- 10 1.75E- 08 C1orf21 2.22948563 4.45E- 06 0.00013159 19

C1orf228 2.77076047 4.68E- 10 6.43E- 08 C20orf103 2.3526157 1.32E- 07 7.17E- 06 C20orf96 2.34969604 4.40E- 05 0.00085577 C21orf33 2.21047013 6.77E- 07 2.81E- 05 C5orf34 3.13693127 2.12E- 08 1.61E- 06 C5orf54 2.45315 6.21E- 06 0.00017271 C6orf125 3.13555817 3.54E- 12 9.33E- 10 C6orf223 2.69986868 2.30E- 05 0.00050693 C9orf116 3.58401063 4.31E- 05 0.00084382 C9orf40 2.47108201 7.43E- 08 4.45E- 06 CA2 2.21875687 3.71E- 07 1.72E- 05 CACNA2D3 2.63838015 3.15E- 08 2.21E- 06 CAMK2B 7.95202735 3.32E- 08 2.31E- 06 CAMKV 2.86039372 1.40E- 05 0.00033624 CBR4 2.4463628 2.20E- 05 0.00048814 CBS 2.90463871 4.27E- 11 7.84E- 09 CCDC106 3.63776879 1.15E- 13 4.35E- 11 CCDC111 2.17195651 1.93E- 05 0.00043832 CCDC28B 2.28081461 3.15E- 05 0.00065532 CCDC34 2.83400011 4.92E- 09 4.80E- 07 CCDC9 2.02961347 3.03E- 05 0.0006357 CCDC90B 2.47906989 7.57E- 07 3.08E- 05 CD19 3.50645079 8.43E- 06 0.00022351 CD302 2.48696218 9.95E- 08 5.69E- 06 CDC42EP1 2.61620008 4.36E- 09 4.35E- 07 CDC45 2.57371613 7.39E- 09 6.68E- 07 CDK5 2.23459704 1.73E- 06 6.07E- 05 CDT1 2.40188364 2.96E- 08 2.12E- 06 CENPH 2.88578734 3.08E- 08 2.19E- 06 CENPM 2.22850275 8.94E- 07 3.47E- 05 CHAF1A 2.27283673 2.43E- 07 1.21E- 05 CHAF1B 2.41568467 6.61E- 08 4.03E- 06 CHCHD5 2.03059972 3.31E- 05 0.00067813 CHST14 2.30221981 2.71E- 07 1.32E- 05 CHSY3 2.71354665 4.92E- 07 2.16E- 05 CKAP4 2.89839539 3.37E- 11 6.64E- 09 CKS1B 2.33880047 4.04E- 07 1.84E- 05 CLEC3B 2.41411258 6.01E- 06 0.00016775 CNTLN 2.2396848 1.71E- 06 6.02E- 05 COMTD1 2.47968568 1.92E- 05 0.00043757 CREB3L4 2.3637979 9.64E- 07 3.69E- 05 CRELD1 2.22287085 1.66E- 06 5.90E- 05 20

CRIP1 2.44186622 4.54E- 08 2.91E- 06 CRISPLD1 3.48038976 2.82E- 13 9.62E- 11 CRNDE 2.11659502 1.09E- 05 0.00027522 CSPG4 2.95793826 5.25E- 11 9.33E- 09 CSPG5 2.91382099 4.73E- 08 3.00E- 06 CTSG 2.52183488 4.18E- 09 4.23E- 07 CYB5R2 6.29675157 6.77E- 20 1.48E- 16 CYB5R3 2.02118074 8.68E- 06 0.0002287 DACH1 2.34158815 5.71E- 07 2.46E- 05 DDB2 2.34689749 5.37E- 07 2.35E- 05 DDIT3 2.24226151 1.43E- 06 5.20E- 05 DFNA5 2.26438172 3.40E- 05 0.00069476 DHRS3 2.15348543 9.58E- 06 0.00024897 DLEU1 2.24297026 6.69E- 06 0.00018338 DLEU2 2.22692465 2.86E- 05 0.00060846 DNAH11 2.48431527 1.13E- 07 6.37E- 06 DPH5 2.07562921 1.43E- 05 0.00034153 DTYMK 2.39418599 4.11E- 08 2.72E- 06 DUT 2.05044486 5.84E- 06 0.00016356 E2F8 2.04508779 1.15E- 05 0.00028732 EAF2 2.07366288 1.01E- 05 0.00026004 EIF4EBP1 2.11604482 2.64E- 06 8.51E- 05 EME1 2.02568757 3.63E- 05 0.00072667 EMILIN3 3.3817834 1.17E- 07 6.53E- 06 EML2 2.17296405 2.12E- 06 7.14E- 05 EP400NL 2.34627276 3.72E- 07 1.72E- 05 ETFB 2.12113731 2.06E- 06 6.97E- 05 ETV4 5.71842569 9.38E- 15 4.66E- 12 EXOSC8 2.53665028 7.03E- 08 4.26E- 06 FADS2 4.78360773 6.81E- 20 1.48E- 16 FAM116B 2.45869143 6.67E- 07 2.78E- 05 FAM151A 2.10289899 1.36E- 05 0.00032815 FAM189B 2.45066125 1.95E- 08 1.50E- 06 FAM54A 2.4974566 4.01E- 08 2.67E- 06 FAM69B 3.03526516 1.83E- 11 3.97E- 09 FAM82B 2.26809453 7.13E- 07 2.93E- 05 FAM92A1 2.31726305 2.23E- 06 7.39E- 05 FANCG 2.02447261 1.16E- 05 0.00028919 FARSB 2.05694957 5.55E- 06 0.00015759 FBF1 2.51367766 9.49E- 08 5.49E- 06 FBXO4 2.39271333 7.35E- 07 3.01E- 05 FBXO43 2.20982168 4.01E- 05 0.00079297 21

FDPS 2.00401919 9.65E- 06 0.0002503 FKBP9 2.93495158 9.30E- 11 1.52E- 08 FNTB 2.65808034 1.50E- 08 1.21E- 06 G6PC3 2.70865901 5.06E- 10 6.80E- 08 GALE 2.27564606 4.44E- 06 0.00013145 GALK1 2.6175098 2.35E- 09 2.61E- 07 GAMT 2.57861116 3.59E- 09 3.74E- 07 GBAP1 3.20757928 1.27E- 07 6.95E- 06 GCAT 2.49798672 1.07E- 07 6.08E- 06 GCHFR 2.28656205 4.70E- 05 0.00089809 GDF11 4.55867896 1.37E- 15 8.81E- 13 GGCT 2.19219996 1.77E- 06 6.15E- 05 GHDC 2.06881853 4.78E- 06 0.00013899 GINS1 2.10882834 2.62E- 05 0.00056652 GINS4 2.60311958 1.56E- 08 1.24E- 06 GLYCTK 2.64169982 4.18E- 06 0.00012512 GMNN 2.47922484 2.15E- 07 1.10E- 05 GNA15 2.31612393 2.01E- 07 1.04E- 05 GNG4 4.18912634 3.37E- 08 2.34E- 06 GPR114 3.11118647 3.08E- 11 6.24E- 09 GPR153 2.44034463 4.63E- 06 0.00013548 GPR161 3.43525177 9.98E- 06 0.00025708 GPT2 3.71320329 2.57E- 14 1.16E- 11 GSTZ1 2.65138404 2.53E- 08 1.85E- 06 GTPBP3 2.14500217 7.86E- 06 0.00021004 HAAO 2.04250468 4.60E- 05 0.00088408 HAGHL 2.26723745 1.73E- 06 6.07E- 05 HIP1R 2.44886714 8.34E- 06 0.00022175 HIST1H4E 3.00262545 9.13E- 08 5.33E- 06 HMBS 2.83702409 1.79E- 10 2.65E- 08 HMMR 2.2744649 4.54E- 07 2.03E- 05 HSH2D 3.47901071 5.01E- 09 4.87E- 07 IFRD2 2.15155024 1.32E- 06 4.88E- 05 IGFBP4 2.30096808 2.88E- 07 1.38E- 05 IL21R 2.3329881 3.26E- 06 0.0001016 IMPA2 2.22864743 7.62E- 07 3.09E- 05 ING5 2.2157365 1.61E- 05 0.00037869 INTS3 2.82279776 1.16E- 10 1.86E- 08 ITGB4 2.79732653 1.85E- 06 6.33E- 05 KAT2A 3.42885985 5.16E- 14 2.17E- 11 KCNQ3 2.37318892 4.86E- 06 0.00014104 KCNQ4 2.2463028 1.61E- 06 5.77E- 05 22

KHK 2.5960592 6.48E- 07 2.72E- 05 KREMEN2 2.25023196 1.36E- 05 0.00032815 LAMP1 2.5051339 6.55E- 09 6.07E- 07 LAT2 2.05676176 6.00E- 06 0.00016762 LETMD1 2.2119772 4.31E- 06 0.00012818 LOC100128332.42913132 6.31E- 06 0.00017494 LOC285972 3.03861042 1.51E- 08 1.21E- 06 LOC388796 2.12061296 8.96E- 06 0.00023442 LOC440028 4.3573999 7.61E- 07 3.09E- 05 LOC728724 2.25178957 1.47E- 05 0.00034857 LOC81691 2.80589898 2.39E- 09 2.64E- 07 LONP1 2.02865705 6.44E- 06 0.00017757 LRRC34 3.30280316 1.80E- 09 2.06E- 07 LRRC42 2.62732129 3.40E- 09 3.61E- 07 LRRC45 2.72131086 9.95E- 10 1.21E- 07 LRRCC1 2.43611654 3.88E- 08 2.61E- 06 LTBP4 2.08237381 7.04E- 06 0.00019169 LTK 2.96120175 2.19E- 05 0.0004853 LYPLAL1 2.4048538 2.48E- 07 1.23E- 05 MACROD1 3.36902572 1.02E- 08 8.81E- 07 MANF 2.46880478 1.79E- 08 1.38E- 06 MAP4K1 2.28450312 2.53E- 07 1.24E- 05 MAPK12 2.06059924 1.36E- 05 0.00032815 MCM2 2.39634463 2.87E- 08 2.07E- 06 MCM3 2.30692585 1.07E- 07 6.08E- 06 MCM5 2.00829677 8.68E- 06 0.0002287 MCM6 2.1300863 1.58E- 06 5.69E- 05 MEI1 3.67567504 2.93E- 13 9.81E- 11 MELK 2.01935951 1.86E- 05 0.00042593 METTL7B 2.01498585 1.24E- 05 0.00030649 MGAT4B 2.24770424 3.59E- 07 1.68E- 05 MGC23270 2.27381137 3.16E- 06 9.91E- 05 MGST2 2.16719007 1.28E- 06 4.75E- 05 MLH1 2.10847143 3.22E- 06 0.00010054 MLST8 2.2292633 5.50E- 07 2.39E- 05 MMAB 2.29034963 1.21E- 06 4.55E- 05 MND1 2.93400701 7.58E- 09 6.82E- 07 MPDU1 2.1108628 3.66E- 06 0.0001119 MRM1 2.99019656 9.46E- 10 1.17E- 07 MRPL20 2.48253861 1.42E- 08 1.16E- 06 MRPL24 2.28629202 2.23E- 07 1.13E- 05 MS4A6A 2.51620547 6.81E- 09 6.27E- 07 23

MTHFD1 2.45287959 1.67E- 08 1.32E- 06 MYBL2 2.53260298 3.84E- 09 3.96E- 07 MYBPH 2.62517355 1.76E- 09 2.02E- 07 MYH14 2.11673845 2.89E- 06 9.18E- 05 N6AMT2 2.06992945 3.56E- 05 0.00071794 NAPSB 2.7040167 8.75E- 10 1.09E- 07 NCF2 3.27457697 2.21E- 12 6.04E- 10 NCS1 2.79202532 2.11E- 08 1.60E- 06 NDUFA7 2.96141953 5.52E- 11 9.58E- 09 NFU1 2.0341778 2.45E- 05 0.00053741 NIT2 2.45053978 4.37E- 08 2.81E- 06 NPM3 2.46851712 4.88E- 07 2.15E- 05 NPRL3 3.08545844 7.78E- 12 1.83E- 09 NUDT22 2.22077012 8.08E- 07 3.22E- 05 OBFC2B 2.18952466 1.86E- 06 6.36E- 05 OIP5 2.55246168 1.56E- 07 8.23E- 06 OSGEPL1 2.31601104 2.81E- 05 0.00060052 PACSIN3 3.86738147 3.10E- 08 2.19E- 06 PAF1 34.0840002 9.68E- 68 2.12E- 63 PAFAH1B3 2.89723762 8.21E- 11 1.38E- 08 PAQR4 2.03576568 1.08E- 05 0.00027272 PCCB 3.02220185 6.20E- 12 1.52E- 09 PCDH12 2.09459546 5.07E- 05 0.00095425 PCDH7 2.18205838 1.61E- 06 5.77E- 05 PCK2 2.3287386 1.84E- 07 9.60E- 06 PHF14 2.72491359 7.14E- 10 9.20E- 08 PHF19 2.14604459 1.84E- 06 6.31E- 05 PHGDH 2.19862021 6.12E- 07 2.60E- 05 PIH1D1 2.05867622 5.69E- 06 0.00016069 PKMYT1 2.21406438 5.55E- 07 2.40E- 05 PLAC8 2.48490863 8.69E- 09 7.63E- 07 POLA2 4.27987811 3.63E- 18 4.17E- 15 POLE2 2.66483578 6.50E- 07 2.72E- 05 PPIB 2.10900165 1.93E- 06 6.58E- 05 PPP1R14B 2.09955489 5.17E- 06 0.00014868 PRDX2 2.01035481 9.12E- 06 0.00023809 PRPS1 2.02445873 1.24E- 05 0.00030639 PRR19 3.07668264 1.39E- 10 2.20E- 08 PRTN3 2.24482016 2.47E- 07 1.22E- 05 PSAT1 2.23986301 8.10E- 07 3.23E- 05 PSPH 2.33557091 3.86E- 07 1.77E- 05 PTPRF 2.37291914 4.07E- 08 2.70E- 06 24

PYCR1 2.28501416 2.31E- 07 1.17E- 05 R3HDM2 2.28604728 2.82E- 07 1.36E- 05 RAC3 4.50301158 6.73E- 16 5.07E- 13 RAD23A 2.20052836 5.76E- 07 2.48E- 05 RAD51 2.26690446 6.67E- 07 2.78E- 05 RAD54L 3.67875691 1.78E- 15 1.08E- 12 RANGRF 2.54786987 6.14E- 07 2.60E- 05 RFC4 2.25287757 8.56E- 07 3.38E- 05 RHBDL1 2.8159667 3.93E- 07 1.80E- 05 RIBC2 2.89145837 4.89E- 09 4.80E- 07 RIPK3 2.25765739 4.36E- 06 0.00012964 RLTPR 4.88526585 6.33E- 06 0.00017522 RNASEH2B 2.02153651 1.03E- 05 0.00026242 ROBO3 2.35436749 1.23E- 06 4.60E- 05 RPA3 2.88299657 1.26E- 09 1.51E- 07 RPL26L1 2.05832012 3.49E- 05 0.00070708 RPS26 3.7362957 1.30E- 09 1.53E- 07 RRP7A 2.03066572 9.77E- 06 0.00025308 RTKN 2.13719608 1.97E- 05 0.00044768 RUVBL1 2.02376577 8.71E- 06 0.00022912 SARS2 3.48519565 2.96E- 13 9.81E- 11 SERPINB2 2.04596442 8.85E- 06 0.00023216 SHF 5.23934025 1.31E- 05 0.0003198 SIRT5 2.75920423 6.23E- 07 2.63E- 05 SLC17A9 2.05591736 1.13E- 05 0.00028454 SLC24A1 2.1571809 2.21E- 05 0.0004893 SLC25A10 2.29555289 3.23E- 07 1.54E- 05 SLC2A4RG 2.05029923 4.90E- 06 0.00014169 SLC37A4 2.0696373 1.66E- 05 0.00038726 SLC39A4 2.0170028 1.37E- 05 0.00033052 SMC1B 2.61810483 7.45E- 07 3.04E- 05 SNHG11 2.16841662 5.24E- 05 0.00097887 SORBS3 2.34642392 1.32E- 07 7.18E- 06 SPNS3 2.85289179 1.43E- 10 2.26E- 08 SPTB 3.74682787 2.42E- 10 3.50E- 08 ST3GAL4 2.04215186 8.45E- 06 0.00022382 STRA13 2.26581499 5.84E- 06 0.00016356 STYXL1 2.01335745 1.52E- 05 0.00036003 TCF7 3.42156573 1.22E- 11 2.73E- 09 TEC 2.28899688 1.97E- 06 6.71E- 05 TERT 5.72768577 1.93E- 09 2.19E- 07 TFB1M 2.22509179 5.22E- 06 0.00014938 25

TGM4 2.62192297 3.00E- 05 0.00063163 TIFAB 2.26066369 7.10E- 06 0.00019258 TIMM10 2.37394643 1.46E- 07 7.79E- 06 TIPIN 2.05524874 2.49E- 05 0.00054414 TLCD1 2.84346127 8.77E- 06 0.00023048 TMBIM4 2.06653078 6.39E- 06 0.00017663 TMEM194A 2.31861152 4.60E- 07 2.05E- 05 TMEM38B 2.02118204 1.60E- 05 0.00037674 TOE1 2.01119397 1.43E- 05 0.00034127 TRAP1 2.05778135 4.49E- 06 0.00013245 TRIM65 2.20976436 1.34E- 06 4.93E- 05 TRPT1 2.81134444 1.11E- 09 1.34E- 07 TYSND1 2.52495377 7.01E- 07 2.89E- 05 UBE2T 2.4300657 5.84E- 08 3.60E- 06 UXT 2.22621813 1.21E- 06 4.53E- 05 VEGFB 2.84220447 5.31E- 10 7.08E- 08 VILL 3.08466906 1.53E- 10 2.37E- 08 WDR4 2.07602605 1.84E- 05 0.0004227 YEATS4 2.1469276 7.95E- 06 0.00021218 ZCWPW1 3.46074026 2.02E- 10 2.96E- 08 ZNF114 2.29699677 5.10E- 06 0.00014705 ZNF219 2.40063166 4.77E- 07 2.11E- 05 ZNF429 2.02389151 3.81E- 05 0.00075972 ZNF642 2.02961599 7.22E- 06 0.00019506 ZNF692 2.04348001 1.88E- 05 0.00042891 ZSWIM5 3.30313838 3.27E- 05 0.00067309 26

Upregulated (FC >2) #GeneName FC(WT/KD) P- value PDR ABCA1 0.4574029 1.77E- 06 6.15E- 05 ABCA7 0.37218851 1.72E- 09 1.99E- 07 ABL2 0.46907624 3.25E- 06 0.00010136 ABTB1 0.35425344 1.67E- 09 1.94E- 07 ABTB2 0.23567588 2.97E- 18 3.61E- 15 ADAM9 0.34311945 3.55E- 11 6.93E- 09 ADAMTS15 0.48805242 1.67E- 05 0.00039075 ADAMTS4 0.24523489 2.00E- 13 7.28E- 11 ADRB1 0.44940189 5.61E- 07 2.42E- 05 AFF4 0.42826872 1.29E- 07 7.06E- 06 AHNAK 0.35581193 6.43E- 11 1.11E- 08 AHNAK2 0.26089357 2.80E- 16 2.27E- 13 AKAP13 0.48509429 7.31E- 06 0.00019702 ALDH8A1 0.16794216 3.51E- 08 2.41E- 06 ANKRD36BP1 0.46716411 4.21E- 06 0.00012587 ANPEP 0.48895342 4.79E- 06 0.00013907 AOC2 0.09679032 6.18E- 17 5.40E- 14 AOC3 0.03979219 2.37E- 11 5.04E- 09 AP4S1 0.30649172 1.31E- 08 1.08E- 06 APC2 0.43034261 5.61E- 07 2.42E- 05 APOL6 0.49071113 1.21E- 05 0.00030213 ARHGAP31 0.46719474 4.15E- 06 0.00012446 ARID3B 0.37638989 2.34E- 06 7.70E- 05 ARID3C 0.12714925 4.02E- 12 1.05E- 09 ARID4A 0.48030523 1.11E- 05 0.00028012 ARID4B 0.49898574 1.31E- 05 0.0003198 ARRDC4 0.33113884 1.59E- 11 3.50E- 09 ATG14 0.46916022 3.68E- 05 0.00073402 ATP6V0A1 0.48533297 7.45E- 06 0.00020025 ATP7A 0.33831869 2.26E- 09 2.52E- 07 ATXN1L 0.47128298 3.22E- 06 0.00010054 AVPR2 0.24960691 3.61E- 07 1.68E- 05 B3GNT4 0.0255409 7.05E- 10 9.17E- 08 B3GNT8 0.49985375 1.32E- 05 0.00032064 BAIAP3 0.12533686 8.85E- 09 7.74E- 07 BCAS3 0.4464389 2.90E- 06 9.19E- 05 BCL2L11 0.31217334 3.43E- 12 9.16E- 10 BCL6 0.4598684 2.66E- 06 8.54E- 05 BCL9L 0.38286896 2.15E- 09 2.42E- 07 27

BMF 0.1786909 2.60E- 19 3.79E- 16 BTG2 0.10239121 2.80E- 37 2.04E- 33 C10orf10 0.27060046 2.85E- 07 1.37E- 05 C10orf55 0.42376968 4.62E- 05 0.00088742 C12orf35 0.4390568 2.51E- 07 1.24E- 05 C12orf51 0.46766152 1.81E- 06 6.23E- 05 C17orf63 0.48429696 7.72E- 06 0.00020654 C1orf38 0.46235144 1.85E- 06 6.33E- 05 C21orf90 0.24792735 2.07E- 05 0.00046482 C3orf25 0.14304387 9.54E- 10 1.17E- 07 C3orf62 0.31370102 1.04E- 08 8.94E- 07 C5orf41 0.34988375 2.16E- 08 1.63E- 06 C8orf73 0.40745618 3.07E- 06 9.65E- 05 C9orf172 0.42499194 4.97E- 06 0.00014363 CA14 0.16237981 3.04E- 06 9.57E- 05 CCDC144NL 0.34863947 1.19E- 08 9.93E- 07 CCNG2 0.41998474 3.58E- 07 1.68E- 05 CCR10 0.05746038 7.40E- 08 4.44E- 06 CD164 0.49475992 7.66E- 06 0.00020523 CDC42BPB 0.48586417 6.78E- 06 0.00018551 CDC42EP3 0.40737536 2.21E- 08 1.66E- 06 CDKN1A 0.29119919 1.06E- 13 4.07E- 11 CEBPD 0.42231864 5.83E- 08 3.60E- 06 CEBPE 0.44210887 1.11E- 06 4.20E- 05 CELSR3 0.41487956 2.46E- 05 0.00053963 CITED4 0.47271444 1.77E- 06 6.15E- 05 CLDN12 0.33949241 4.13E- 10 5.72E- 08 CNTNAP1 0.15967099 1.70E- 08 1.33E- 06 CPD 0.48479785 4.11E- 06 0.00012374 CRB2 0.15344338 4.83E- 07 2.13E- 05 CREG1 0.39328647 4.20E- 09 4.23E- 07 CSF2RB 0.49241672 9.91E- 06 0.00025622 CSGALNACT2 0.44112211 5.56E- 06 0.00015759 CSRNP2 0.44752667 1.41E- 06 5.12E- 05 CTSB 0.4496119 3.84E- 07 1.77E- 05 CX3CR1 0.49123471 1.02E- 05 0.00026149 CYP1B1 0.32049034 9.54E- 13 2.86E- 10 DAAM1 0.37547352 5.75E- 08 3.56E- 06 DCUN1D3 0.29968448 2.68E- 09 2.93E- 07 DICER1 0.34602669 4.75E- 11 8.52E- 09 DLL4 0.2049359 7.54E- 20 1.48E- 16 DMPK 0.35381435 3.67E- 06 0.00011223 28

DNAJB13 8.51E- 10 5.66E- 09 5.41E- 07 DUSP6 0.35750335 1.62E- 10 2.48E- 08 DYNLL2 0.39569829 1.09E- 08 9.30E- 07 EGR3 0.23165442 2.85E- 06 9.08E- 05 EHD2 0.43112556 2.13E- 07 1.09E- 05 ENC1 0.29112153 6.71E- 13 2.07E- 10 ENO3 0.37088215 5.20E- 05 0.00097422 EOMES 0.2635628 1.77E- 12 5.02E- 10 EPHB3 0.44339206 3.35E- 06 0.00010399 EPM2AIP1 0.40581918 1.20E- 07 6.68E- 06 EVI2B 0.3449396 3.83E- 11 7.23E- 09 EVI5 0.47935562 1.85E- 05 0.00042502 F11R 0.40644238 4.56E- 08 2.92E- 06 FAM20C 0.48041881 4.31E- 06 0.00012818 FAM43A 0.41917785 4.30E- 08 2.78E- 06 FAM53C 0.41882093 4.72E- 08 3.00E- 06 FBXL18 0.46973739 3.39E- 06 0.00010471 FBXO39 0.10316531 5.16E- 06 0.00014856 FLJ39639 0.29230031 5.39E- 08 3.37E- 06 FLNB 0.4817536 3.36E- 06 0.00010407 FLT1 0.23639403 9.38E- 06 0.0002443 FMNL2 0.38282126 2.91E- 06 9.22E- 05 FNDC3A 0.41259608 3.44E- 08 2.38E- 06 FOS 0.39177495 4.91E- 09 4.80E- 07 FOSL2 0.35358206 8.13E- 11 1.38E- 08 FOXO1 0.31996287 4.68E- 11 8.45E- 09 FOXO3 0.32262652 5.14E- 12 1.31E- 09 FRS2 0.44202005 1.67E- 06 5.92E- 05 FSCN2 0.2376116 8.64E- 07 3.39E- 05 FTH1 0.4277402 7.46E- 08 4.45E- 06 FUT4 0.41091356 1.70E- 08 1.33E- 06 GAB2 0.39508528 1.70E- 08 1.33E- 06 GAL3ST4 0.04138273 4.57E- 06 0.00013407 GALNT4 0.40446852 3.32E- 07 1.57E- 05 GGN 0.13443653 1.30E- 06 4.80E- 05 GJC2 0.14596546 4.98E- 15 2.53E- 12 GLS 0.45397737 7.97E- 07 3.19E- 05 GNAT2 0.13032335 5.92E- 17 5.40E- 14 GP1BB 0.47815639 2.18E- 05 0.00048497 GPX3 0.22339073 4.30E- 08 2.78E- 06 GRIN3B 0.44019039 2.58E- 06 8.35E- 05 H1F0 0.21236467 1.39E- 21 4.34E- 18 29

HBEGF 0.41328712 5.64E- 06 0.00015962 HIC2 0.49435803 2.09E- 05 0.00046889 HIPK2 0.44857743 5.98E- 07 2.56E- 05 HIST1H2AC 0.18553296 1.28E- 12 3.74E- 10 HIST1H2AG 0.21146939 7.45E- 07 3.04E- 05 HIST1H2BD 0.26423631 1.35E- 07 7.27E- 06 HIST2H2BE 0.20991194 1.16E- 08 9.79E- 07 HIST2H2BF 0.31670634 6.64E- 10 8.70E- 08 HOXA9 0.46045392 1.35E- 06 4.96E- 05 HRH2 0.49632923 1.50E- 05 0.0003552 IER2 0.49155118 1.01E- 05 0.00026004 IER5L 0.33408556 1.19E- 11 2.68E- 09 IFI30 0.45796359 8.71E- 07 3.40E- 05 IL10RA 0.35931233 2.89E- 10 4.10E- 08 IL6ST 0.38263806 8.04E- 09 7.15E- 07 IQCD 0.36971518 7.97E- 07 3.19E- 05 ITGA5 0.41605525 2.51E- 08 1.85E- 06 ITGAV 0.46877464 2.75E- 06 8.83E- 05 ITGAX 0.39039964 7.06E- 09 6.44E- 07 JMY 0.4646921 7.24E- 06 0.00019535 JUN 0.43037452 1.52E- 07 8.08E- 06 KBTBD11 0.42317687 7.19E- 08 4.33E- 06 KCND1 0.18311608 1.07E- 15 7.06E- 13 KCNG2 0.19132308 2.91E- 05 0.00061749 KDM5B 0.46667389 1.29E- 06 4.78E- 05 KIAA1033 0.43100264 2.34E- 07 1.17E- 05 KIAA1432 0.45137818 6.23E- 07 2.63E- 05 KIAA1683 0.20721889 4.00E- 08 2.67E- 06 KLF10 0.44112477 2.58E- 07 1.26E- 05 KLF13 0.40463374 2.79E- 08 2.02E- 06 KLF2 0.17925258 1.30E- 17 1.36E- 14 KLF4 0.48180478 5.81E- 06 0.00016326 KLHL11 0.46608436 5.29E- 06 0.00015111 KLHL18 0.47445441 5.67E- 06 0.00016015 KLHL28 0.40649616 2.31E- 06 7.62E- 05 LAMP2 0.49839249 8.91E- 06 0.00023347 LFNG 0.36056443 1.11E- 06 4.20E- 05 LGMN 0.21561967 2.27E- 06 7.52E- 05 LMTK2 0.47204052 4.67E- 06 0.00013662 LOC100216540.29372217 1.81E- 06 6.23E- 05 LOC100268160.12380771 5.00E- 05 0.00094501 LOC100289010.32858008 8.20E- 08 4.82E- 06 30

LOC283392 0.43688558 2.64E- 07 1.29E- 05 LOC401463 0.32513657 1.65E- 10 2.50E- 08 LOC645431 0.32639018 1.37E- 05 0.00032984 LOC90246 0.48639194 1.75E- 05 0.00040521 LONRF3 0.49889049 3.29E- 05 0.00067662 LSP1 0.48616273 1.46E- 05 0.00034614 LUZP1 0.48578746 6.52E- 06 0.00017926 LYN 0.46695729 1.47E- 06 5.31E- 05 MAML3 0.32544615 1.76E- 10 2.62E- 08 MAP1A 0.25777867 3.50E- 09 3.68E- 07 MAP1LC3A 0.4051845 1.75E- 07 9.17E- 06 MAP3K2 0.47367329 1.03E- 05 0.00026377 MCL1 0.4562074 6.38E- 07 2.68E- 05 MGEA5 0.45814285 1.27E- 06 4.73E- 05 MICALL2 0.46812507 4.89E- 06 0.00014169 MKNK2 0.45176215 4.93E- 07 2.16E- 05 MMP2 0.35405306 8.75E- 11 1.46E- 08 MT2A 0.26807284 1.84E- 15 1.09E- 12 MTSS1 0.48624475 5.15E- 05 0.00096784 MYO1E 0.43404969 2.57E- 07 1.26E- 05 MYSM1 0.47952858 2.78E- 05 0.00059574 NCOA2 0.48262596 4.43E- 06 0.00013145 NCOA3 0.34496427 5.43E- 11 9.49E- 09 NEAT1 0.38756555 6.16E- 09 5.76E- 07 NFAT5 0.44893356 3.35E- 06 0.00010399 NOTCH2 0.42243207 4.34E- 08 2.80E- 06 NOXO1 0.22414326 6.21E- 09 5.78E- 07 OLIG1 0.17512421 9.47E- 20 1.59E- 16 OR2B11 0.24286119 2.39E- 06 7.83E- 05 OTP 0.31867061 2.05E- 05 0.00046126 OVGP1 0.15432009 3.64E- 08 2.49E- 06 PALLD 0.36206889 2.16E- 06 7.20E- 05 PAQR3 0.46476879 5.55E- 06 0.00015759 PAQR6 0.15175211 3.59E- 09 3.74E- 07 PBXIP1 0.47204749 2.55E- 06 8.28E- 05 PDE7B 0.42239178 6.62E- 07 2.77E- 05 PGAM2 0.2339851 2.08E- 13 7.45E- 11 PGAP1 0.36331051 4.33E- 05 0.00084514 PHLDA1 0.33400642 1.43E- 07 7.65E- 06 PHLDA2 0.37736046 2.53E- 08 1.85E- 06 PHTF2 0.41473021 3.69E- 07 1.71E- 05 PIGW 0.44285226 2.32E- 06 7.64E- 05 31

PLAU 0.43578991 5.51E- 07 2.39E- 05 PLEKHO2 0.3328954 9.67E- 12 2.23E- 09 PLIN4 0.2076805 1.64E- 06 5.84E- 05 PNRC1 0.44319695 2.84E- 07 1.37E- 05 PPP1R3F 0.32594727 1.57E- 08 1.25E- 06 PRDM1 0.47662928 7.01E- 06 0.00019112 PRDM2 0.43825148 2.24E- 07 1.14E- 05 PRIC285 0.31222343 5.12E- 13 1.62E- 10 PRICKLE4 0.43442575 2.57E- 05 0.00055868 PSPN 0.19025066 4.89E- 10 6.69E- 08 PTAFR 0.32198602 8.05E- 06 0.00021451 PTGDS 0.3509822 6.18E- 10 8.19E- 08 PURB 0.44732981 8.34E- 07 3.31E- 05 RAB11FIP1 0.31948789 1.10E- 12 3.26E- 10 RAB3D 0.4643283 2.46E- 06 8.02E- 05 RBM47 0.40004871 1.78E- 08 1.38E- 06 RC3H1 0.47828804 3.90E- 06 0.00011784 RGAG4 0.4049391 9.33E- 07 3.59E- 05 RGL1 0.40647663 2.19E- 05 0.0004855 RHOB 0.21096937 1.48E- 19 2.31E- 16 RNF149 0.45712185 1.46E- 06 5.29E- 05 RNF213 0.45429242 6.04E- 07 2.58E- 05 SASH1 0.3964055 5.61E- 09 5.38E- 07 SEC23A 0.33732922 7.54E- 11 1.29E- 08 SECISBP2L 0.4150844 9.32E- 08 5.42E- 06 SERPINI1 0.26501065 3.20E- 11 6.36E- 09 SERTAD1 0.21854292 7.67E- 18 8.38E- 15 SESN1 0.33990255 5.35E- 11 9.43E- 09 SETD7 0.46642293 5.19E- 06 0.0001488 SGK1 0.32311124 5.99E- 08 3.67E- 06 SH2B3 0.4006707 1.32E- 08 1.09E- 06 SH3BP5 0.36325727 1.84E- 09 2.09E- 07 SKIL 0.48343034 1.02E- 05 0.00026149 SLC12A6 0.39750151 2.29E- 08 1.71E- 06 SLC30A1 0.46687968 1.74E- 06 6.09E- 05 SLC34A3 0.14665496 2.39E- 06 7.85E- 05 SLC37A2 0.47063811 4.07E- 06 0.00012285 SLC4A5 0.13227755 1.16E- 05 0.00029047 SOCS1 0.16721511 2.61E- 15 1.39E- 12 SPOPL 0.48809442 2.60E- 05 0.00056339 SPRED3 9.65E- 10 5.19E- 08 3.25E- 06 SPSB1 0.39702193 1.20E- 07 6.68E- 06 32

SPTBN5 0.11469641 1.42E- 12 4.07E- 10 SPTY2D1 0.47418527 3.62E- 06 0.00011095 SSBP3 0.47000653 7.41E- 06 0.00019926 SSPO 0.30732197 1.40E- 08 1.15E- 06 STAB1 0.04310987 1.26E- 05 0.0003102 STAT2 0.4139249 5.46E- 08 3.40E- 06 STK17B 0.45737088 1.35E- 06 4.94E- 05 SYNPO 0.44209226 3.85E- 07 1.77E- 05 SYT2 0.12870576 8.63E- 07 3.39E- 05 TAGAP 0.46188389 2.78E- 06 8.89E- 05 TAS1R3 0.2343633 8.43E- 06 0.00022351 TBC1D2 0.44928032 1.01E- 06 3.86E- 05 TBC1D8 0.46494879 1.60E- 06 5.76E- 05 TCHH 0.48677935 3.86E- 05 0.00076885 TCTE1 0.03700895 6.13E- 07 2.60E- 05 TEP1 0.41328506 3.23E- 08 2.25E- 06 TG 0.25041361 8.10E- 09 7.17E- 07 TGFBR2 0.31015451 6.85E- 13 2.08E- 10 THBD 0.44187872 2.33E- 07 1.17E- 05 THBS2 0.15578638 1.55E- 05 0.00036558 THSD1 0.09366748 1.94E- 08 1.49E- 06 TLR4 0.42547402 1.15E- 07 6.43E- 06 TMCO2 0.05405277 1.00E- 05 0.00025836 TNFRSF10B 0.43792925 4.46E- 07 2.00E- 05 TNFRSF10D 0.36011124 5.40E- 07 2.35E- 05 TNFRSF1B 0.45808715 9.14E- 07 3.54E- 05 TNFSF13B 0.39041081 2.15E- 07 1.10E- 05 TNFSF9 0.29373222 2.35E- 13 8.30E- 11 TNRC6A 0.47451366 3.28E- 06 0.00010214 TOB1 0.39380124 1.11E- 08 9.39E- 07 TOB2 0.38877701 3.60E- 09 3.74E- 07 TP53I3 0.47699021 3.55E- 05 0.00071721 TP53INP1 0.13720899 9.83E- 16 6.94E- 13 TPP1 0.44843351 3.31E- 07 1.57E- 05 TREML1 0.4118781 2.87E- 05 0.00061 TRERF1 0.41024735 2.21E- 08 1.66E- 06 TRHDE 0.46072498 1.16E- 06 4.36E- 05 TRIB1 0.26797987 2.67E- 13 9.25E- 11 TRIM23 0.40211115 4.34E- 07 1.95E- 05 TSPYL4 0.47379906 1.24E- 05 0.00030639 TSSK2 0.33451833 3.03E- 08 2.16E- 06 TTC14 0.47177786 2.15E- 05 0.00048038 33

TULP4 0.40872805 4.25E- 08 2.77E- 06 TWF1 0.33762804 4.84E- 08 3.05E- 06 TXNIP 0.47110049 1.58E- 06 5.69E- 05 UBASH3B 0.41538699 9.62E- 08 5.52E- 06 UBFD1 0.42074227 1.68E- 07 8.83E- 06 UBXN7 0.4823463 1.40E- 05 0.00033624 UHMK1 0.43061674 1.14E- 07 6.39E- 06 VAMP3 0.46430928 2.03E- 05 0.00045989 VASN 0.36804515 2.62E- 08 1.91E- 06 VSIG4 0.06395154 5.55E- 41 6.07E- 37 WASL 0.47290438 1.75E- 05 0.00040575 WDR47 0.41563249 2.06E- 07 1.06E- 05 WDR48 0.49629678 2.85E- 05 0.00060821 WNK4 0.22748877 1.02E- 07 5.82E- 06 YOD1 0.41208676 2.43E- 07 1.21E- 05 YPEL3 0.35389474 9.40E- 08 5.45E- 06 ZAR1 0.37699283 6.05E- 09 5.70E- 07 ZBED6 0.41436295 6.90E- 08 4.19E- 06 ZC3HAV1 0.48391428 4.29E- 06 0.00012814 ZFAND5 0.46683671 1.77E- 06 6.15E- 05 ZFP106 0.49278 7.57E- 06 0.00020314 ZFP36L1 0.2108341 1.40E- 18 1.85E- 15 ZFYVE1 0.43015842 2.09E- 07 1.08E- 05 ZNF497 0.40994664 7.08E- 07 2.92E- 05 ZNFX1 0.47579191 2.78E- 06 8.89E- 05 ZSWIM4 0.47645216 2.96E- 05 0.00062412 34

Downregulated (FC >1.5) #GeneName FC(WT/KD) P- value PDR 5- Sep 1.92733417 5.44E- 05 0.00100737 6- Sep 2.04087268 1.42E- 05 0.00034008 AAAS 1.76127081 0.00037954 0.00471618 AARS 2.45141433 1.42E- 08 1.16E- 06 AARSD1 1.73373482 0.00062311 0.00691749 ABCB8 1.74750265 0.00071785 0.00772603 ACAT1 1.79154246 0.00021824 0.00305957 ACAT2 2.2692087 3.27E- 07 1.55E- 05 ACBD4 2.03247471 0.00053598 0.0061857 ACCS 1.88535721 0.00015609 0.00235371 ACD 1.75904649 0.0004414 0.00529533 ACOT11 2.0430517 1.72E- 05 0.00040111 ACOT13 1.95618208 0.00010998 0.00177125 ACOT7 2.1605579 1.72E- 06 6.03E- 05 ADCK2 2.25391867 4.32E- 07 1.95E- 05 ADM2 4.52831048 2.22E- 06 7.39E- 05 ADSL 1.71323913 0.00060303 0.00674252 AES 2.00318551 1.29E- 05 0.00031694 AHCY 1.88798087 4.97E- 05 0.00094072 AIG1 2.63994465 1.28E- 09 1.52E- 07 AIMP2 1.97345087 2.06E- 05 0.00046384 AKR7A2 2.12051254 2.13E- 06 7.14E- 05 ALDH7A1 2.00976862 0.00064638 0.00712184 ALKBH3 2.05599539 1.31E- 05 0.0003198 ANKLE1 2.61701517 8.25E- 09 7.27E- 07 ANKRD39 1.86802272 0.00059364 0.00666478 APRT 1.99082644 1.21E- 05 0.00030213 ARHGAP22 3.43916168 0.00028501 0.00375945 ARHGAP9 1.83811608 0.00014203 0.00218752 ARHGEF16 2.18603362 0.00016336 0.0024353 ARHGEF19 1.84099784 0.00061781 0.00687259 ARL6IP6 2.86446876 2.27E- 10 3.32E- 08 ARMC6 1.84726445 0.00010454 0.00169737 ARMC7 2.17843364 9.05E- 06 0.00023635 ARNTL2 1.97237975 0.00011406 0.00182749 ARPC4 1.72509069 0.00050132 0.00586925 ARRDC1 1.81320993 0.00017142 0.00253474 ASF1A 1.91120523 6.14E- 05 0.00110831 ASF1B 2.11120021 2.76E- 06 8.84E- 05 35

ASPHD1 1.88039842 8.53E- 05 0.00144515 ASS1 1.76916689 0.00027349 0.00363828 ATAD3A 1.70999842 0.00080191 0.00840741 ATF4 3.81051626 1.09E- 16 9.18E- 14 ATF5 1.95883314 2.05E- 05 0.00046126 ATIC 2.18333403 7.75E- 07 3.13E- 05 ATP5G1 1.7202604 0.00057545 0.00650389 ATP5I 1.70421661 0.00078466 0.00826216 ATP8B3 2.30371628 3.53E- 07 1.66E- 05 AXL 2.16656077 6.08E- 06 0.00016951 B3GNT7 2.48008906 1.70E- 08 1.33E- 06 B3GNT9 2.05065277 0.00020573 0.00293306 B3GNTL1 1.86236718 0.00044533 0.00533085 B4GALT2 2.80894292 1.67E- 10 2.52E- 08 BAD 1.751244 0.00074403 0.00796469 BAG2 2.08064505 6.49E- 06 0.00017869 BAI1 2.66671941 0.00027439 0.0036465 BANF1 1.83095079 0.00015984 0.00239109 BATF 2.121087 5.19E- 06 0.0001488 BATF3 2.83640531 3.44E- 08 2.38E- 06 BCAT1 1.85839765 0.00017976 0.0026349 BDH1 2.65452812 7.85E- 09 7.01E- 07 BEND5 3.56609113 4.50E- 06 0.00013266 BLM 1.73203731 0.00056291 0.00640853 BLVRA 1.94577933 3.25E- 05 0.00067163 BRCA1 2.19989044 6.73E- 07 2.79E- 05 BRIP1 2.53396693 5.97E- 08 3.67E- 06 BTBD2 2.40109185 3.46E- 08 2.38E- 06 C12orf10 2.100941 4.70E- 06 0.00013714 C12orf11 1.81227992 0.00021131 0.00299115 C14orf149 2.86092168 3.66E- 08 2.49E- 06 C14orf159 2.01541929 1.41E- 05 0.00033748 C14orf80 1.89350079 9.73E- 05 0.00161243 C16orf13 1.92892323 3.29E- 05 0.00067678 C16orf53 1.78535933 0.00027083 0.00360723 C16orf58 1.90636185 5.48E- 05 0.00101168 C16orf59 2.69902067 4.03E- 09 4.12E- 07 C16orf93 2.43531615 2.64E- 07 1.29E- 05 C17orf53 1.86513519 0.00018864 0.00274486 C19orf48 1.80862317 0.00015996 0.00239126 C19orf54 2.06626661 1.37E- 05 0.0003294 C19orf57 2.09965038 0.00090499 0.00932714 36

C19orf70 2.21252472 1.05E- 06 3.97E- 05 C1orf200 3.01461117 1.08E- 10 1.75E- 08 C1orf21 2.22948563 4.45E- 06 0.00013159 C1orf228 2.77076047 4.68E- 10 6.43E- 08 C1orf56 2.26058311 7.05E- 05 0.00124461 C20orf103 2.3526157 1.32E- 07 7.17E- 06 C20orf27 1.93536466 3.59E- 05 0.00072185 C20orf96 2.34969604 4.40E- 05 0.00085577 C21orf33 2.21047013 6.77E- 07 2.81E- 05 C2orf28 1.75090847 0.00038198 0.00473044 C5orf20 1.98949615 0.00017627 0.00259598 C5orf34 3.13693127 2.12E- 08 1.61E- 06 C5orf35 2.19820534 0.00029416 0.00386147 C5orf39 1.84056308 0.00069679 0.00752538 C5orf54 2.45315 6.21E- 06 0.00017271 C6orf108 1.7114149 0.0006962 0.00752271 C6orf125 3.13555817 3.54E- 12 9.33E- 10 C6orf130 2.00544323 0.00019538 0.00281604 C6orf223 2.69986868 2.30E- 05 0.00050693 C6orf57 1.89066168 0.0002438 0.00332202 C7orf50 1.70789571 0.00081215 0.00850255 C9orf100 1.95137873 4.21E- 05 0.00082663 C9orf116 3.58401063 4.31E- 05 0.00084382 C9orf123 1.75313993 0.00058382 0.00658486 C9orf142 1.80802046 0.00023021 0.00318962 C9orf40 2.47108201 7.43E- 08 4.45E- 06 C9orf64 1.79968788 0.00052787 0.00610822 CA2 2.21875687 3.71E- 07 1.72E- 05 CA7 3.75053746 0.00033863 0.00431577 CACNA2D3 2.63838015 3.15E- 08 2.21E- 06 CALR 1.94473551 2.04E- 05 0.00046074 CAMK2B 7.95202735 3.32E- 08 2.31E- 06 CAMKV 2.86039372 1.40E- 05 0.00033624 CARD9 1.79221601 0.00022137 0.00308442 CARHSP1 1.78885837 0.00025465 0.00344417 CATSPER1 1.81577395 0.00056125 0.00640285 CBR4 2.4463628 2.20E- 05 0.00048814 CBS 2.90463871 4.27E- 11 7.84E- 09 CCDC106 3.63776879 1.15E- 13 4.35E- 11 CCDC111 2.17195651 1.93E- 05 0.00043832 CCDC138 2.16394472 0.00010204 0.00166753 CCDC163P 2.32329612 5.79E- 05 0.00106006 37

CCDC28B 2.28081461 3.15E- 05 0.00065532 CCDC34 2.83400011 4.92E- 09 4.80E- 07 CCDC9 2.02961347 3.03E- 05 0.0006357 CCDC90B 2.47906989 7.57E- 07 3.08E- 05 CCNB2 1.69269474 0.00087881 0.00908292 CCNE2 1.96473258 4.48E- 05 0.00086752 CD151 1.78054432 0.00026897 0.00358686 CD19 3.50645079 8.43E- 06 0.00022351 CD302 2.48696218 9.95E- 08 5.69E- 06 CD5 3.236498 0.00053291 0.00615353 CDC42EP1 2.61620008 4.36E- 09 4.35E- 07 CDC45 2.57371613 7.39E- 09 6.68E- 07 CDC6 1.89699837 5.65E- 05 0.00103922 CDCA7 1.92103631 4.87E- 05 0.00092625 CDCA7L 1.96526087 2.72E- 05 0.00058555 CDK1 1.77505733 0.00041447 0.00503299 CDK2 1.98501598 2.44E- 05 0.00053646 CDK5 2.23459704 1.73E- 06 6.07E- 05 CDK5RAP3 1.87204702 0.00011528 0.00184292 CDT1 2.40188364 2.96E- 08 2.12E- 06 CENPH 2.88578734 3.08E- 08 2.19E- 06 CENPM 2.22850275 8.94E- 07 3.47E- 05 CENPP 1.99962134 0.0002896 0.00381074 CENPW 1.78978553 0.0003412 0.00434087 CETN3 2.1786187 9.60E- 05 0.00159158 CHAF1A 2.27283673 2.43E- 07 1.21E- 05 CHAF1B 2.41568467 6.61E- 08 4.03E- 06 CHCHD3 1.82762661 0.00016404 0.0024438 CHCHD4 1.79843798 0.00050816 0.00591772 CHCHD5 2.03059972 3.31E- 05 0.00067813 CHST14 2.30221981 2.71E- 07 1.32E- 05 CHSY3 2.71354665 4.92E- 07 2.16E- 05 CIB3 4.07157682 0.0006826 0.00740861 CISD3 1.84399547 0.00014025 0.00216926 CKAP4 2.89839539 3.37E- 11 6.64E- 09 CKS1B 2.33880047 4.04E- 07 1.84E- 05 CLEC3B 2.41411258 6.01E- 06 0.00016775 CLN6 1.99036927 1.32E- 05 0.00032072 CMC1 1.87512628 0.0001511 0.00229162 CNTLN 2.2396848 1.71E- 06 6.02E- 05 CNTROB 1.87946236 9.40E- 05 0.00156393 COMTD1 2.47968568 1.92E- 05 0.00043757 38

COPZ2 2.15140367 0.00026578 0.00354854 COQ5 1.71407783 0.00091377 0.0094043 COQ6 1.74758116 0.00098439 0.00996234 COX7A2 1.84003687 9.79E- 05 0.00161785 CPNE9 5.25001616 0.00010127 0.00165901 CPSF2 1.70263489 0.00076078 0.0080886 CREB3L4 2.3637979 9.64E- 07 3.69E- 05 CRELD1 2.22287085 1.66E- 06 5.90E- 05 CRIP1 2.44186622 4.54E- 08 2.91E- 06 CRISPLD1 3.48038976 2.82E- 13 9.62E- 11 CRNDE 2.11659502 1.09E- 05 0.00027522 CSPG4 2.95793826 5.25E- 11 9.33E- 09 CSPG5 2.91382099 4.73E- 08 3.00E- 06 CTNNAL1 1.99370248 3.60E- 05 0.00072331 CTSG 2.52183488 4.18E- 09 4.23E- 07 CUL1 1.69789101 0.00078009 0.00822593 CXCR2 1.75129668 0.00061342 0.00683421 CYB5R2 6.29675157 6.77E- 20 1.48E- 16 CYB5R3 2.02118074 8.68E- 06 0.0002287 CYP4V2 1.93041576 0.00040785 0.00497084 D2HGDH 1.7758321 0.00087621 0.00906472 DACH1 2.34158815 5.71E- 07 2.46E- 05 DCLRE1B 1.78007457 0.00032915 0.00423697 DCPS 1.9834419 1.45E- 05 0.00034611 DCTPP1 1.8624174 0.00010541 0.00171019 DDB2 2.34689749 5.37E- 07 2.35E- 05 DDIT3 2.24226151 1.43E- 06 5.20E- 05 DDX11 1.86684605 0.00042286 0.00512356 DEAF1 1.74158606 0.0004837 0.00571508 DERL3 1.9985458 0.00010415 0.00169376 DFNA5 2.26438172 3.40E- 05 0.00069476 DHRS11 2.08667176 0.00046098 0.00549391 DHRS13 1.77674125 0.00064875 0.00714047 DHRS3 2.15348543 9.58E- 06 0.00024897 DLEU1 2.24297026 6.69E- 06 0.00018338 DLEU2 2.22692465 2.86E- 05 0.00060846 DNAH11 2.48431527 1.13E- 07 6.37E- 06 DOK4 2.06794409 0.0007374 0.00790148 DPH5 2.07562921 1.43E- 05 0.00034153 DSCC1 1.91426061 9.24E- 05 0.00154217 DSN1 1.91181991 0.00015548 0.00234822 DTL 1.77870925 0.00026353 0.00353156 39

DTYMK 2.39418599 4.11E- 08 2.72E- 06 DUS3L 1.70824276 0.00073009 0.00783463 DUT 2.05044486 5.84E- 06 0.00016356 E2F1 1.80843254 0.00023285 0.00321091 E2F8 2.04508779 1.15E- 05 0.00028732 EAF2 2.07366288 1.01E- 05 0.00026004 EIF4EBP1 2.11604482 2.64E- 06 8.51E- 05 EME1 2.02568757 3.63E- 05 0.00072667 EMG1 1.8816378 0.00010434 0.00169536 EMILIN3 3.3817834 1.17E- 07 6.53E- 06 EML2 2.17296405 2.12E- 06 7.14E- 05 EML3 1.96120047 3.04E- 05 0.00063724 ENOPH1 1.79428639 0.00025275 0.00342056 ENOSF1 2.00107001 5.59E- 05 0.00102997 EP400NL 2.34627276 3.72E- 07 1.72E- 05 EPS8L1 1.80094806 0.00085435 0.00887212 ERCC5 1.68966986 0.00095258 0.00970641 ERMAP 1.69159984 0.0009256 0.00949922 ETFB 2.12113731 2.06E- 06 6.97E- 05 ETNK2 3.84841456 0.00012058 0.00191654 ETV4 5.71842569 9.38E- 15 4.66E- 12 EXOSC8 2.53665028 7.03E- 08 4.26E- 06 FADS1 1.98720843 2.38E- 05 0.00052333 FADS2 4.78360773 6.81E- 20 1.48E- 16 FAF1 1.70094622 0.00071951 0.00773628 FAHD2A 2.05404966 8.72E- 05 0.00147124 FAM116B 2.45869143 6.67E- 07 2.78E- 05 FAM151A 2.10289899 1.36E- 05 0.00032815 FAM162A 1.78707047 0.00038191 0.00473044 FAM163A 1.81783437 0.00040302 0.00493236 FAM189B 2.45066125 1.95E- 08 1.50E- 06 FAM192A 1.87295613 0.0001021 0.00166753 FAM54A 2.4974566 4.01E- 08 2.67E- 06 FAM69B 3.03526516 1.83E- 11 3.97E- 09 FAM82B 2.26809453 7.13E- 07 2.93E- 05 FAM92A1 2.31726305 2.23E- 06 7.39E- 05 FANCD2 1.74188189 0.00055312 0.00633002 FANCG 2.02447261 1.16E- 05 0.00028919 FANCI 1.90362167 5.14E- 05 0.0009657 FARSA 1.87459254 6.22E- 05 0.00112048 FARSB 2.05694957 5.55E- 06 0.00015759 FBF1 2.51367766 9.49E- 08 5.49E- 06 40

FBXO4 2.39271333 7.35E- 07 3.01E- 05 FBXO43 2.20982168 4.01E- 05 0.00079297 FCHO1 1.988246 3.14E- 05 0.00065444 FDPS 2.00401919 9.65E- 06 0.0002503 FEN1 1.68375583 0.00089366 0.00922342 FH 1.79231542 0.00021354 0.00301487 FHOD1 1.78741428 0.00026295 0.00352799 FKBP9 2.93495158 9.30E- 11 1.52E- 08 FNTB 2.65808034 1.50E- 08 1.21E- 06 FUT7 1.95747297 2.31E- 05 0.00050874 G6PC3 2.70865901 5.06E- 10 6.80E- 08 GALE 2.27564606 4.44E- 06 0.00013145 GALK1 2.6175098 2.35E- 09 2.61E- 07 GAMT 2.57861116 3.59E- 09 3.74E- 07 GAS5 1.91284205 4.99E- 05 0.00094405 GBAP1 3.20757928 1.27E- 07 6.95E- 06 GCAT 2.49798672 1.07E- 07 6.08E- 06 GCHFR 2.28656205 4.70E- 05 0.00089809 GDF11 4.55867896 1.37E- 15 8.81E- 13 GGCT 2.19219996 1.77E- 06 6.15E- 05 GHDC 2.06881853 4.78E- 06 0.00013899 GINS1 2.10882834 2.62E- 05 0.00056652 GINS2 1.75194021 0.00058884 0.00662445 GINS4 2.60311958 1.56E- 08 1.24E- 06 GLDC 2.10764761 0.00015749 0.00237006 GLTPD1 1.78323616 0.00042826 0.00518032 GLYCTK 2.64169982 4.18E- 06 0.00012512 GMNN 2.47922484 2.15E- 07 1.10E- 05 GMPS 1.81837341 0.00014167 0.00218575 GNA15 2.31612393 2.01E- 07 1.04E- 05 GNAL 3.24651688 0.00015885 0.00238275 GNG4 4.18912634 3.37E- 08 2.34E- 06 GPM6B 2.48523331 6.33E- 05 0.00113537 GPR114 3.11118647 3.08E- 11 6.24E- 09 GPR153 2.44034463 4.63E- 06 0.00013548 GPR160 1.75395081 0.00033238 0.00426095 GPR161 3.43525177 9.98E- 06 0.00025708 GPSM1 1.79168343 0.00052902 0.00611823 GPT2 3.71320329 2.57E- 14 1.16E- 11 GRIP1 2.0993243 0.00015977 0.00239109 GSS 1.75806126 0.00039711 0.0048709 GSTZ1 2.65138404 2.53E- 08 1.85E- 06 41

GTPBP3 2.14500217 7.86E- 06 0.00021004 GUF1 1.87524307 0.00013926 0.00215855 HAAO 2.04250468 4.60E- 05 0.00088408 HADH 1.79796361 0.00020501 0.00292657 HADHA 1.70842298 0.00059867 0.00670911 HAGHL 2.26723745 1.73E- 06 6.07E- 05 HDDC2 1.87593507 7.50E- 05 0.00130108 HHLA3 1.89552941 0.00031004 0.00403131 HINT2 1.89378673 0.00010909 0.00175948 HIP1R 2.44886714 8.34E- 06 0.00022175 HIRIP3 1.92930876 4.09E- 05 0.00080639 HIST1H4A 2.38039915 0.00022929 0.00317983 HIST1H4E 3.00262545 9.13E- 08 5.33E- 06 HIST2H2AC 2.49654471 0.00019513 0.00281496 HK2 1.76767471 0.00033503 0.00428487 HMBS 2.83702409 1.79E- 10 2.65E- 08 HMMR 2.2744649 4.54E- 07 2.03E- 05 HSD17B7 1.87962975 0.00076723 0.0081334 HSF2BP 2.51266946 0.00046625 0.00553879 HSH2D 3.47901071 5.01E- 09 4.87E- 07 HSPB11 1.96737652 4.34E- 05 0.00084692 HSPE1 1.81550917 0.00021151 0.00299205 IAH1 1.8401617 0.00015616 0.00235371 IFRD2 2.15155024 1.32E- 06 4.88E- 05 IGFBP4 2.30096808 2.88E- 07 1.38E- 05 IL21R 2.3329881 3.26E- 06 0.0001016 IMPA2 2.22864743 7.62E- 07 3.09E- 05 IMPDH2 1.96633784 1.62E- 05 0.00037998 ING2 1.87559187 0.0002435 0.00332202 ING5 2.2157365 1.61E- 05 0.00037869 INTS3 2.82279776 1.16E- 10 1.86E- 08 INTS7 1.90603949 4.96E- 05 0.00093951 INTS8 1.84072235 0.00012692 0.00199557 INVS 1.83036187 0.00023191 0.00320402 IRAK1 1.78246578 0.00022063 0.0030773 ISOC2 1.96190059 2.68E- 05 0.00057823 ITGB3BP 2.26556618 0.00050634 0.00590914 ITGB4 2.79732653 1.85E- 06 6.33E- 05 ITPR3 1.9432915 0.00010262 0.00167243 KAT2A 3.42885985 5.16E- 14 2.17E- 11 KATNAL2 2.46281737 0.00015881 0.00238275 KCNK2 4.5747577 0.00018497 0.0027005 42