NES: normalized enrichment score (analyzed using KEGG pathway gene sets in the GSEA software); FDR:

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1 Supplementary table1. List of gene sets with simultaneously altered the enrichment upon SAP18 overexpression and knockdown. Gene sets enriched by SAP18 and reduced by shsap18 SAP18 against LacZ shsap18 against shscrbl Gene set NAME NES FDR q-val NES FDR q-val KEGG_ARACHIDONIC_ACID_METABOLISM KEGG_CELL_CYCLE KEGG_DNA_REPLICATION KEGG_HOMOLOGOUS_RECOMBINATION KEGG_HUNTINGTONS_DISEASE KEGG_MISMATCH_REPAIR KEGG_NUCLEOTIDE_EXCISION_REPAIR KEGG_OOCYTE_MEIOSIS KEGG_OXIDATIVE_PHOSPHORYLATION KEGG_P53_SIGNALING_PATHWAY KEGG_PARKINSONS_DISEASE KEGG_PURINE_METABOLISM KEGG_PYRIMIDINE_METABOLISM KEGG_RNA_DEGRADATION KEGG_SPLICEOSOME Gene sets reduced by SAP18 and enriched by shsap18 SAP18 against LacZ shsap18 against shscrbl Gene set NAME NES FDR q-val NES FDR q-val KEGG_AMYOTROPHIC_LATERAL_SCLEROSIS_ALS KEGG_B_CELL_RECEPTOR_SIGNALING_PATHWAY KEGG_ENDOMETRIAL_CANCER KEGG_FC_EPSILON_RI_SIGNALING_PATHWAY KEGG_GNRH_SIGNALING_PATHWAY KEGG_LONG_TERM_POTENTIATION KEGG_OLFACTORY_TRANSDUCTION KEGG_PROTEIN_EXPORT KEGG_RENAL_CELL_CARCINOMA KEGG_VASCULAR_SMOOTH_MUSCLE_CONTRACTION NES: normalized enrichment score (analyzed using KEGG pathway gene sets in the GSEA software); FDR: false discovery rate (only gene sets with FDR<25% are shown).

2 Supplementary table2. List of genes with simultaneously altered the expression (over 2-fold and P<0.05) upon SAP18 overexpression and knockdown. SAP18 against LacZ shsap18 against shscrbl Gene Symbol Fold change ANOVA p-value Fold Change ANOVA p-value Acat Ap3b Arl5b Atp2b Atrx Bfar Cab Cdc14b Cideb Clcn Clip Cpne Csnk2a Cyp2a Ddx Dhx Dnajc Efr3a Eif2d Enpp Enpp Enpp Entpd Etf Fads Fam126b Fth Gcc Gclc Gm Golga Hdlbp

3 Hspa Jak Jhdm1d Kif5b Klf Mapkapk Mmgt Mtmr Mttp Ncl Odc Pcmtd Pggt1b Phf Ppargc1a Ppp2r5c Ptplad Rbm Rnf Rora Rps Scaf Secisbp2l Sfxn Slc25a Stt3b Tbl1x Tmem176a Tnpo Topors Usp Xpnpep Zfp Ear Fabp Gm

4 Lyz Mbl Psmc Rasgrp Saa Tardbp

5 A Rela9ve;expression; level;against;control 4; 3; 2; 1; Sap18;mRNA;levels;in;mice;liver * shsap18; shscrbl; LacZ+Cre; SAP18+Cre; B Sap18 βac9n shsap18 shscrbl LacZ+Cre Sap18+Cre HA6tagged intrinsic Rela9ve;band;intensity 2; 1.5; 1; 0.5; intrinsic;sap18; HA6SAP18; shsap18; shscrbl; LacZ+Cre; SAP18+Cre; Supplementary fig.1. SAP18 expression level in fasting mouse liver administrated by adenovirus vectors. A. Sap18 mrna levels in mouse liver injected with respective adenoviral vector at 8th date post administration. Relative expression levels against internal control 36B4 normalized to the mean value of shscrbl are shown (mean±sd, n=6-8). B. SAP18 protein levels in mouse liver injected with respective adenoviral vector at 8th date post administration are shown by western blotting stained with anti-sap18. The chemiluminescence of the western filter stained with anti-sap18 and anti-βactin were developed using SuperSignal West Femto and Pico Substrate (Life technologies), respectively. Bar graph shows the relative band intensities normalized to the mean value of shscrbl (mean±sd). Abbreviations: shsap18, knockdown vector encoding shrna template (pax-shsap18); shscrbl, control vector encoding non-specific targeting shrna template; LacZ+Cre, control vector encoding LacZ (paxcalnlz2) with paxcalncre; SAP18+Cre, overexpression vector (paxcalnlwtit2-sap18) with paxcalncre. P <0.01 (Student s t-test).

Representative LacZ expression in a mouse

6 Heart Liver Spleen Lung Pancreas Small;intes9ne Kidney Supplementary fig.2. Representative LacZ expression in a mouse co-injected with paxcalnlz2 and paxcalncre adenoviral vectors at 8th date post administration. The fozen sections of the seven organs were fixed with 0.5% glutarldehyde and stained by X-Gal. The scale bars indicate 100 µm.

7 (mg/dl) 10 T.Chol (mg/dl) 20 TG shsap18; 8 * * 15 Scrbl; LacZ+Cre; 6 SAP18+Cre; Fed; Fas9ng; Fed; Fas9ng; Supplementary fig.3. Total cholesterol and triglyceride concentrations in plasma at day 4 of adenoviral vector-injected mice. Blood samples were collected from the tail veins of vector injected mice (mean±sd, n=6-8 per group) under conditions of food intake ad libitum (Fed) or after 12 h of starvation (Fasting). Abbreviations: shsap18, knockdown vector encoding shrna template (pax-shsap18); shscrbl, control vector encoding non-specific targeting shrna template; LacZ+Cre, control vector encoding LacZ (paxcalnlz2) with paxcalncre; SAP18+Cre, overexpression vector (paxcalnlwtit2-sap18) with paxcalncre. *P <0.05

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