1 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. //, No.+, +/ (,**2) 1 Mechanism of the Inactivation of Microorganisms by Non-thermal Process Soichi Furukawa Department of Food Science and Technology, College of Bioresource Sciences, Nihon University + -*/** MPa +, ++,/* MPa, -* - AcrAB-,/,2/+* +200 Corresponding author furukawa@brs.nihon-u.ac.jp TolC. AcrAB-TolC Nikaido / Tet. AcrAB-TolC - Tet + + 1/ MPa, -* DNA, ++* FtsZ 0 SOS SOS - 0 FtsZ +, FtsZ bo -*
2 // +,**2 + 2 +** MPa 1 1/ MPa 2 /* MPa 3. + +- +**-** MPa +* +* / - + - AcrAB-TolC OM : outer membrane, CM : cytoplasmic membrane.., 1/ MPa, -1C -* min Escherichia coli K-+, + pressure sensitive point biological e#ect E. coli S. cerevisiae S. pombe 1/ MPa +** MPa 1/ MPa FtsZ ring Actin cable Contractile ring (actin cytoskeleton) cell elongation (cell division inhibition) budding inhibition fission inhibition
3 : 3 / Bacillus subtilis RP : reciprocal pressurization, CP : continuous pressurization. ++ -* MPa +, +. 0 HT HPT HCT + Escherichia coli K-+, DNA, CO, CO, --* MPa +-+/,+ CO, CO, DNA 0 +0 DNA DNA DNA +0 CO, CO, +1 1,, ATPase ATPase CO, V-H - ATPase H ph P-H -ATPase H ph V-H -ATPase P-H -ATPase CO, V-H -ATPase P-H -ATPase CO, 1 +2,- CO, CO,
4 // +,**2 + 4 2 HCT Bacillus coagulans 2 +3 CO, CO,,*,. CO, +**,+ +** +** + +323, +323 - /* +./+.2,**.. Kawarai, T., Ogihara, H., Aono, R., Kishima, M., Inagi, Y., Irie, A., Ida, A., Furukawa, S. and Yamasaki, M., High hydrostatic pressure treatment impairs AcrAB-TolC pump resulting in the di#erential loss of deoxycholatetolerance in Escherichia coli. J. Biosci. Bioeng., +**, 0+- 0+0 (,**0). / Thanassi, D.G.., Cheng, L.W. and Nikaido, H., Active e%ux of bile salts by Escherichia coli. J. Bacteriol., +13,,/+,,/+2 (+331). 0 Kawarai, T., Wachi, M., Ogino, H., Furukawa, S., Suzuki, K., Ogihara, H. and Yamasaki, M., SulA independent filamentation of Esherichia coli during growth after release from high hydrostatic pressure treatment. Appl. Microbiol. Biotechnol., 0.,,//,0, (,**.). 1 Kawarai, T., Arai, S., Furukawa, S., Ogihara, H. and Yamasaki, M., High hydrostatic pressure treatment impairs actin cables and budding in Saccharomyces cerevisiae. J. Biosci. Bioeng., +*+, /+//+2 (,**0). 2,**. p. +01,**. 3 Sato, T., Miwa, T., Ishii, A., Kato, C., Wachi, M., Nagai, K., Aizawa, M. and Horikoshi, K., The dynamism of Esche-
5 : 5 +* ++ +, +- +. +/ richia coli under high hydrostatic pressure-repression of the FtsZ-ring formation and chromosomal DNA condensation. In Trends in high pressure bioscience and biotechnology, ed. Hayashi, R., (Elsevier science BV, Tokyo), pp.,--,-2 (,**,). Furukawa, S., Nakahara, A. and Isao Hayakawa, I., E#ect of reciprocal pressurization on germination and killing of bacterial spores. Int. J. Food Sci. Technol., -/, /,3/-, (,***). Furukawa, S., Shimoda, M. and Hayakawa, I., Mechanism for the inactivation of bacterial spores by reciprocal pressurization treatment. J. Appl. Microbiol., 3., +0 (,**-). Furukawa, S. and Isao Hayakawa, I., E#ect of temperature on the inactivation of Bacillus stearothermophilus IFO +,//* spores by low hydrostatic pressure treatment. Biocontrol Sci., 0, ---0 (,**+). Ishikawa, H., Shimoda, M., Tamaya, K., Yonekura, A., Kawano, T. and Osajima, Y., Inactivation of Bacillus spores by the supercritical carbon dioxide micro-bubble method. Biosci. Biotechnol. Biochem., 0+, +*,,+*,- (+331). Dillow, A.K., Dehghani, F., Hrkach, J.S., Foster, N.R. and Langer, R., Bacterial inactivation by using near- and supercritical carbon dioxide. Proc. Nat. Acad. Sci. U.S. A., 30, +*-..+*-.2 (+333). Nakamura, K., Enomoto, A., Fukuhisa, H., Nagai, K. and Hakoda. M., Disruption of microbial cells by the flash discharge of high-pressure carbon dioxide. Biosci. Biotechnol. Biochem., /2, +,31+-*+ (+33.). +0 Watanabe, T., Furukawa, S., Kawarai, T., Wachi, M., Ogihara, H. and Yamasaki, M., Cytoplasmic acidification may occur in high-pressure carbon dioxide-treated Escherichia coli K+,. Biosci. Biotechnol. Biochem., in press. +1 /- p. +,2,**0 +2 Watanabe, T., Furukawa, S., Kitamoto, K., Takatsuki, A., Hirata, R., Ogihara, H. and Yamasaki, M., Vacuolar H -ATPase and plasma membrane H -ATPase contribute to the tolerance against high-pressure carbon dioxide treatment in Saccharomyces cerevisiae, Int. J. Food Microbiol., +*/, +-++-1 (,**/). +3 Furukawa, S., Watanabe, T., Tai, T., Hirata, J., Narisawa, N., Kawarai, T., Ogihara, H. and Yamasaki, M., E#ect of high pressure gaseous carbon dioxide on the germination of bacterial spores. Int. J. Food Microbiol., 3+,,*3,+- (,**.).,* Watanabe, T., Furukawa, S., Hirata, J., Koyama, T., Ogihara, H. and Yamasaki, M., Inactivation of Geobacillus stearothermophilus spores by high pressure carbon dioxide treatment. Appl. Env. Microbiol., 03, 1+,. 1+,3. (,**-).,+ +332 +3 3 +3