THE EFFECT O F THE MYCEL IA O F CORD YCEPS M I L ITAR IS O N REGULATIO N O F H IGH BLOOD L I P ID

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1 () May 2008 JOURNAL OF SOUTH CH INA NORMAL UN IVERSITY (NATURAL SC IENCE ED ITION) No. 2, 2008 : (2008) , 13, 2 (1., ; 2., ) :, 135 KM 9 ( n = 15 / ) : ( 0114 g/kg bw /d 0128 g/kg bw /d 0156 g/kg bw /d)., 1615 ml /kg, (3310 ml /kg), 21 d. 7 d 1, ( TC), 21, TC ( TG) (HDL - C), (LDL - C). : 21, TC TG( P < 0105), ( P < 0101). : ; ; : S Q : A THE EFFECT O F THE MYCEL IA O F CORD YCEPS M I L ITAR IS O N REGULATIO N O F H IGH BLOOD L I P ID WU Yan - m ing 1, HUANG W en - fang 13, L IN Xiang 2 (1. School of L ife Science, South China Normal University, Guangzhou , China; 2. Hosp ital of South China Normal University, Guangzhou , China) Abstract: The experim ent was carried out to investigate the effect of mycelia of Cordyceps m ilitaris on regulating high blood lip id of m ice. One hundred and thirty - five KM m ice were random ly assigned to nine group s ( n = 15 in each group ) : base control group, high lip id model group, positive control group, the dosage of g/kg bw /day mycelia of Cordyceps m ilitaris which was p re - heated or not. W hile the m ice of base control group were received only distilled water of the same cubage by gavage, the others were received high lip id emulsion 1615 ml /kg in the morning and different samp les 3310 ml /kg in the afternoon by gavage everyday and total for 21 days. The body weigh of m ice were weighed every 7 days. The total cholesterol( TC) of serum of the blood which was extracted from the : : ( ),,, 2004 ; ( ),,,, :, zhangshl@ scnu. edu. cn. 3

2 112 ( ) 2008 tails was measured on the 0 th day 7 th day and 14 th day. On the 21 th day, the m ice were dis2 sected after taking out the blood from the m iceπs eyeballs. The TC triglyeride ( TG) high - density lipop rotein cholesterol ( HDL - C) of serum were measured and low - density lipo2 p rotein cholesterol(ldl - C) and liver weigh indices were calculated on the 21 th day. The results suggested that the serum TC TG of high lip id m ice which were treated with mycelia of Cordyceps m ilitaris ( heated or not) and J in - shuibao ( the positive control group) was sig2 nificantly decreased on the 21 th day( P < 0105). The liver weigh indices of the group s trea2 ted w ith mycelia of Cordyceps m ilita ris ( heated or not) extremely and significantly decreased ( P < 0101). Key words: Cordyceps m ilitaris; Mycelia; H igh B lood L ip id ( ) [ 1 ].,,.,1 700,, 440 [ 2 ]. (Cordyceps m ilita ris link), ( Eum ycota), (Ascom ycotinia ), ( Pyrenom ycetes), ( Sphaeria2 les), (C laviciptiaceae), (Cordycps).,,, SOD, [ 3-5 ]., [ 6 ]. [ 7 ] mg/g [ 3 ] mg/l [ 8 ]. (mycelia),, [ 9 ].,, ( ) ( 100) mg/ml (0156 g kg - 1 bw - 1 ) 8140 mg/ml (0128 g kg - 1 bw - 1 ) 4120 mg/ml (0114 g kg - 1 bw - 1 ) 32. 1, m in ( ), mg/ml (0162 g kg - 1 bw - 1 ). (: ).

3 2 : [ 10 ] : 5 g 1 g 015 g ml 11 ml11 g,82 ml,, 1,, SPF ( KM), 135,, g,, : (2005) A : : (: ). : AMRESCO (: ). : (: ). - 80: sigma (: PB11099). :. :(: ). :(: ). : (: ). : (: ). AR. ::. PW SIO :. H. H. S4:. TGL - 16G - A :. BCD - 268W :. V IS - 723G :. JA1203 :. :. :. V IRTIS - 6K:V IRTIS (23 3), (65 5) %1,TC, 9,15. 1, 9: ml /kg,6: 00, 3310 ml /kg,.. / 1 / ( g kg - 1 bw - 1 )

4 114 ( ) , , r/ m in12 m in, 4, 3 d. 1, ,,. 21, r/m in12 m in, 4, 3 d ( TC) ( TG) (HDL - C) (LDL - C) LDL - CFriedewald [ 11 ] : LDL - C = TC - [ HDL - C + TG ] (L iver weigh index) [ 12 ] : 1. 8 = ( ). DPS,, t ,,2. 2 ( gx s, g) / /g : 3 ( P < 0105), 3 3 ( P < 0101)

5 2 : d TC d, TC ( P < ( gx s) 0101),,3. TC / (mmol L - 1 ) 2. 3TC / 0 7 0,TC , TC ( P < 0105)., ( P < 0101),4. : 3 ( P < 0105), 3 3 ( P < 0101) TC ( gx s, mmol/l) / TC : 3 ( P < 0105), 3 3 ( P < 0101) 2. 4TG 21, TG( P < 0105), HDL - C 21,HDL - C ( P < 0105),HDL - C, LDL - C 21, LDL - C ( P < 0105),LDL - C, ,( P < 0101), 5.

6 116 ( ) TG HDL - C LDL - C ( gx s, mmol/l) / TG HDL - C LDL - C : 3 ( P < 0105), 3 3 ( P < 0101) 3,. [ 13, 14 ]. LDL - C,,. : (1) 21,TC TG, [ 15 ] g/ ( kg bw),,,.,,. (2),.. (3), TC TG. (4).,.,, TG,TG, TG,, [ 16 ]., [ 17 ]. : [ 1 ] HUANGA T H W, PENG G, L I G Q, et al. Salacia oblonga root imp roves postp randial hyperlip idem ia and he2 patic steatosis in Zucker diabetic fatty rats: activation of PPAR - a[ J ]. Toxicology and App lied Pharmacology, 2006, 210 (3) :

7 2 : 117 [ 2 ] MCRAE M P. Treatment of hyperlipop roteinem ia with pantethine: a review and analysis of efficacy and tolera2 bility[ J ]. Nutrition Research, 2005, 25 (4) : [ 3 ] HUANG S J, TSA I S Y, LEE Y L, et al. Nonvolatile taste components of fruit bodies and mycelia of Cordycep s m ilitaris[ J ]. Swiss Society of Food Science and Technology, 2006, 39 (6) : [ 4 ] MASUDA M, URABE E, HONDA H, et al. Enhanced p roduction of cordycep in by surface culture using the me2 dicinal mushroom Cordycep s m ilitaris[ J ]. Enzyme and M icrobial Technology, 2007, 40 (5) : [ 5 ] RAO Y K, CHOU C H, TZENG Y M. A simp le and rap id method for identification and determ ination of cordycep in in Cordycep s m ilitaris by cap illary electrophoresis[ J ]. Analytica Chim ica Acta, 2006, 566 ( 2) : [ 6 ],,,. [ J ]., 2005, 26 ( 9) : [ 7 ] ZHAO L W, X IAO X W, W E I Y C. Inhibitory effect of Cordycep s sinensis and Cordycep s m ilitaris on human glomerular mesangial cell p roliferation induced by native LDL [ J ]. Cell B iochem istry and Functions, 2000, 18 (2) : [ 8 ] SH IH IL, TSA I K L, HSIEH C Y. Effects of culture conditions on the mycelial growth and bioactive metabo2 lite p roduction in submerged culture of Cordycep s m ilitaris[ J ]. B iochem ical Engineering Journal, 2007, 33 ( 3) : [ 9 ],,,. [ J ]., 2004, 16 (5) : [ 10 ],,,. [ J ]., 2004, 35 (6) : [ 11 ],,. [ J ]., 2006, 21 (4) : [ 12 ],,,. [ J ]., 2003, 11 (8) : [ 13 ]. [ J ]., 2006, 8 (7) : 107. [ 14 ]. ( ) [ J ]., 2005, 24 (3) : [ 15 ],,,. [ J ]., 2004, 16 (5) : [ 16 ],,,. [ J ]., 2003, 11 (8) : [ 17 ],,,. [ J ]., 2005, 26 ( 2) :

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