DENNIS SYNETOS CURRICULUM VITAE

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1 DENNIS SYNETOS CURRICULUM VITAE Personal details Address: Date and place of birth: Department of Biochemistry, School of Medicine, University of Patras, Patras, Greece. Tel , Fax URL November 13, 1950 in Athens, Greece Studies B.Sc. in Natural Sciences (Direction Biology), University of Athens, Greece, 1974 Ph.D. in Biochemistry, University of Sussex, England, U.K., 1978 Academic positions Research and Teaching Associate, Department of Biochemistry, School of Medicine, University of Patras, Greece, Lecturer, Department of Biochemistry, School of Medicine, University of Patras, Greece, Visiting Lecturer, Department of Molecular Cell Biology, University of Utrecht, The Netherlands, 1983 (3 months) Visiting Lecturer, Centro de Biologia Molecular, University of Madrid, Spain, 1984 (3 months) Assistant Professor, Department of Biochemistry, School of Medicine, University of Patras, Greece, Visiting Assistant Professor, Department of Cell Biology, Albert Einstein College of Medicine, New York, Associate Professor, Department of Biochemistry, School of Medicine, University of Patras, Greece, Professor, Department of Biochemistry, School of Medicine, University of Patras, Greece, 2006-today Selected Research Interests and Projects The role of the yeast ribosome in the phenomenon of hybrid vigor and heterosis. Effects of mutations in rrna and ribosomal proteins on yeast ribosomal function

2 Studies on the effects of antibiotics and other molecules or factors on the two most important functions of ribosomes, i.e. translational fidelity and catalysis of peptide bond formation A study of the yeast prion model through mutations in termination factor erf1 Relationship between translational fidelity and oxidative stress Funded Research Projects University of Patras, Research Committee 1991, carried out between General Secretariat of Research and Technology of Greece, PENED 1991, carried out between General Secretariat of Research and Technology of Greece, PENED 1995, carried out between General Secretariat of Research and Technology of Greece, Joint Research Program between Greece and Italy, General Secretariat of Research and Technology of Greece and The European Social Fund (ESF), PENED 1999, carried out in University of Patras, Research Committee, program K. Karatheodori 2000, carried out between General Secretariat of Research and Technology of Greece, PENED 2001, carried out between European Social Fund (ESF), Operational Program for Educational and Vocational Training (EPEAEK II), Herakleitos 2002, carried out between University of Patras, Research Committee, program K. Karatheodori 2004, carried out between Supervisor of Ph.D., Master s and Diploma Theses 5 Ph.D. theses 5 Master s theses ca. 10 Diploma dissertations Reviewer in International Journals Molecular Microbiology Biochimica et Biophysica Acta Clinical Biochemistry BMC Genomics Biochemistry

3 Journal of Proteome Research Biochimie Evaluator for Research Grants Evaluator of research proposals from the Italian Ministry of Research and Technology, 2000-present (signed agreement) Advisor to the Italian Ministry of Research and Technology on applications for purchase of laboratory instruments and other equipment (signed agreement) Evaluator of research proposals from the Central System of Health, Ministry of Health, Greece Evaluator of research proposals PENED 2003 from the General Secretariat of Research and Technology of Greece Fellowships Awards Short Term EMBO Fellowship to work at the University of Utrecht, The Netherlands, 1983 Short Term FEBS Fellowship to work at the University of Madrid, Spain in 1984 EMBO fund awarded for organizing the International Workshop on Protein Synthesis at the U of Patras in 1986 Four (4) Awards by the British Biochemical Society to participate in FEBS or IUB International Meetings Long Term (1 year) Albert Einstein College of Medicine Fellowship to work as visiting Assistant Professor International and National Collaborations University of Illinois at Chicago (UIC), USA, Laboratory for Molecular Biology, Professor Susan W. Liebman University of Camerino, Italy, Laboratory of Genetics, Professor Claudio Gualerzi. Max-Planck Institute, Germany, Laboratory of Molecular Genetics, Professor Knud H. Nierhaus. The RH Smith Institute for Plant Sciences and Genetics, The Hebrew University, Rehovot, Israel, Dr. Eyal Fridman. Dependence of the hybrid vigor phenomenon from the ribosome. This collaboration has begun in University of Patras, Greece, School of Medicine, Dept of Biochemistry, Professor D. Kalpaxis. Collaboration on several aspects of antibiotic activity on prokaryotic versus eukaryotic ribosomes. University of Patras, Greece, School of Biology, Professor C. Georgiou. A coolaboration to explore the relationship between protein synthesis and oxidative stress.

4 University of Patras, Greece, School of Pharmacy, Assoc. Professor Lilly Papademetriou. A collaboration on the interaction of nucleolin with ribosomal proteins. The possible involvement of additional factors. Memberships Hellenic Society of Biochemistry and Molecular Biology (HSBMB) Hellenic Society of Biological Sciences (HSBS) Panhellenic Society of Bioscientists American Society of Biochemistry and Molecular Biology (ASBMB) Βiochemical Society of Great Britain Endocrinological Society of Great Britain Lectures by Invitation Albert Einstein College of Medicine, New York, Lecture title: A detailed kinetic analysis of prokaryotic peptidyltransferase. Albert Einstein College of Medicine, New York, Lecture title: On the role of yeast ribosomal protein S7 which is encoded by two genes. University of Camerino, Italy, 2001 Lecture title: Mechanistic aspects of translation inhibition by antibiotics active on yeast ribosomes. University of Camerino, Italy, 2001 Lecture title: Mutational studies may reveal the role of several ribosomal and extra-ribosomal components in eukaryotic ribosome function. School of Medicine, University of Ioannina, Ioannina, Greece, Lecture title: Studies on the eukaryotic ribosome function with the aid of mutations School of Medicine, University of Ioannina, Ioannina, Greece, Lecture title: The ribosome as target for antiviral therapy University of Camerino, Italy, 2010 Lecture title: Changes in Translational Fidelity and Catalytic Activity of Eukaryotic Ribosomes Caused by Mutations Can Reveal the Function of Several Antibiotics in Protein Synthesis Publications 23 articles in Peer Reviewed International Journals Total Impact Factor: Mean Impact Factor = : 23 = Citations: 407

5 Citations/publication: 17.7 H Index: articles in National Journals 18 communications in International Conferences 35 communications in National Conferences List of Publications in Peer Reviewed International Journals 1. Cooper, D.M.F., Synetos, D., Christie, R.B. and Schulster, D. (1976) Studies on the nature of corticotrophin releasing hormone and its partial purification from porcine hypothalami, J. Endocrinol. 71, [IF=3.05, Citations: 19] 2. Synetos, D., Jenkins, G., Cooper, D.M.F. and Schulster, D. (1977) Further studies on the nature of partially purified porcine corticotrophin releasing hormone, J.Endocrinol. 72, 7p-8p [IF=3.05, Citations: 3] 3. Jenkins, G., Synetos, D., Cooper, D.M.F. and Schulster, D. (1977) Preliminary studies on the mechanism of action of corticotrophin releasing hormone. J. Endocrinol. 72, 8p-9p [IF=3.05, Citations: 2] 4. Synetos, D., Bristow, A.F. Jenkins, G., Gillies, G., Lowry, P.J., Cooper, D.M.F. and Schulster, D. (1978) In vitro studies on porcine hypothalamic corticotrophin releasing factor, J. Endocrinol. 77, 53p-54p [IF=3.05, Citations: 4 ] 5. Bristow, A. F., Montague, D. Synetos, D., Jenkins, G., Cockayne, D. and Schulster, D. (1980) An improved methodology for the extraction and partial purification of porcine hypothalamic releasing factor, J. Endocrinol. 84, [IF=3.05, Citations : 10] 6. Synetos, D., Amils, R. and Ballesta, J.P.G. (1986) Photolabelling of protein components in the pactamycin binding site of rat liver ribosomes, Biochim. Biophys. Acta 868, [IF=2.77, Citations: 6] 7. Synetos, D. and Coutsogeorgopoulos, C. (1987) Studies on the catalytic rate constant of ribosomal peptidyltransferase, Biochim. Biophys. Acta 923, [IF=2.77, Citations: 38] 8. Synetos, D. and Coutsogeorgopoulos, C. (1989) Reactivity of the P sitebound donor in ribosomal peptide bond formation, Eur. J. Biochem., 184, [IF=3.79, Citations: 9] 9. Synetos, D., Manolopoulos, V., Atlas, D. and Pipili-Synetos, E. (1991) Human plasma-derived material with clonidine displacing substance

6 (CDS)-like properties contracts the isolated rat aorta. J. Auton. Pharmacol. 11, [IF=1.62, Citations: 22] 10. Theocharis, D. A., Synetos, D., Kalpaxis, D.L., Drainas, D. and Coutsogeorgopoulos, C. (1992) Kinetics of inhibition of peptide bond formation on bacterial ribosomes. Arch. Biochem. Biophys. 292, [IF=2.83, Citations: 14] 11. Synetos, D., Dabeva, M. D. and Warner, J.R. (1992) The yeast ribosomal protein S7 and its genes. J. Biol. Chem. 267, [IF=5.33, Citations: 20] 12. Dinos, G., Synetos, D. and Coutsogeorgopoulos, C. (1993) Interaction between the antibiotic spiramycin and a ribosomal complex active in peptide bond formation. Biochemistry 32, [IF=3.23, Citations: 20] 13. Kallia-Raftopoulos, S., Synetos, D., Ottenheijm, H.C.J., van den Broek, L.A.G.M. and Coutsogeorgopoulos, C. (1996) Sparsomycin and its analogs: a new approach for evaluating their potency as inhibitors of peptide bond formation. Molecular Pharmacology 49, [IF=4.71, Citations: 2] 14. Synetos, D., Frantziou, C. P. and Alksne, L.E. (1996) Mutations in yeast ribosomal proteins S28 and S4 affect the accuracy of translation and alter the sensitivity of the ribosomes to paromomycin. Biochim. Biophys. Acta 1309, [IF=2.29, Citations: 20] 15. Ioannou, M., Coutsogeorgopoulos, C. and Synetos, D. (1998). Kinetics of inhibition of rabbit reticulocyte peptidyltransferase by anisomycin and sparsomycin. Molecular Pharmacology 53, [IF=4.71, Citations: 11] 16. Dresios, J., Derkatch, I. L., Liebman, S. W. and Synetos, D. (2000) Υeast ribosomal protein L24 affects the kinetics of protein synthesis and ribosomal protein L39 improves translational accuracy, while mutants lacking both remain viable. Biochemistry 39, [IF=3.23, Citations: 47] 17. Velichutina, I. V., Dresios, J., Hong, J. Y., Li, C., Mankin, A., Synetos, D. and Liebman, S W. (2000) Mutations in helix 27 of the yeast Saccharomyces cerevisiae 18S rrna affect the function of the decoding center of the ribosome. RNA 6, [IF=5.92, Citations : 25] 18. Dresios, J., Panopoulos, P., Frantziou, C. P. and Synetos, D. (2001) Yeast ribosomal protein deletion mutants possess altered

7 petidyltransferase activity and different sensitivity to cycloheximide. Biochemistry 40, [IF=3.23, Citations: 19] 19. Dresios, J., Panopoulos, P., Suzuki, K., and Synetos, D. (2003) A dispensable yeast ribosomal protein optimizes peptidyltransferase activity and affects translocation. J. Biol. Chem. 278, [IF=5.33, Citations: 14] 20. Panopoulos, P., Dresios, J., and Synetos, D. (2004) Biochemical evidence of translational infidelity and decreased peptidyltransferase activity by a sarcin/ricindomain mutation of yeast 25S rrna. Nucleic Acids Res. 32, [IF=6.88, Citations: 5] 21. Dresios, J., Panopoulos, P. and Synetos, D. (2006) Eukaryotic ribosomal proteins lacking a eubacterial counterpart: important players in ribosomal function. Molecular Microbiology 59, [IF=5.36, Citations: 30] 22. Konstantinidis, T. C., Patsoukis, N., Georgiou, C. D. and Synetos, D. (2006) Translational fidelity mutations in 18S rrna affect the catalytic activity of ribosomes and the oxidative balance of yeast cells. Biochemistry 45, [IF=3.23, Citations: 6] 23. Tselika, S., Konstantinidis, T.C. and Synetos, D. (2008) Two nucleotide substitutions in the A-site of yeast 18S rrna affect translation and differentiate the interaction of ribosomes with aminoglycoside antibiotics. Biochimie 90, [IF=3.90, Citations: 1] 24. Konstantinidis, T. C., Derkatch, I. L., Tselika, S., Liebman, S. W. and Synetos, D. (2010) Yeast ribosomal function including translational fidelity is affected by an allosuppressor mutation in an extra-ribosomal yeast phosphatase. Biochemistry (submitted). denotes corresponding author List of publications in National Journals 1. Synetos, D. and Voorma, H. O. (1984) Purification and preliminary characterization of elf-2 and erf from rat liver, Hellenic Biochem. and Biophys. Soc. (HBBS) Newsletter 21, Synetos, D. (1985) Photoaffinity labelling of rat liver ribosomes with the antibiotic pactamycin, Hellenic Biochem. and Biophys. Soc. (HBBS) Newsletter 22,

8 3. Synetos, D. and Coutsogeorgopoulos, C. (1986) The binding of the initiator F-Met-tRNA to the ribosome activates peptidyltransferase, Hellenic Biochem. and Biophys. Soc. (H.B.B.S) Newsletter 24, Synetos, D. and Coutsogeorgopoulos, C. (1988) The effect of magnesium ions on the activity of ribosomal peptidyltransferase, Hellenic Biochem. Biophys. Soc. (HBBS) Newsletter 27, Synetos, D., Theocharis, D.A., Kalpaxis, D.L., Drainas, D. and Coutsogeorgopoulos, C. (1989) The kinetics of inhibition of ribosomal peptidyltransferase: Comparison between ribosomes adsorbed on cellulose nitrate and ribosomes in solution Hellenic Biochem. Biophys. Soc. (H.B.B.S.) Newsletter 28, Dinos, G., Synetos, D. and Coutsogeorgopoulos, C. (1990) The antibiotic spiramycin forms a tight complex with the 70S ribosome and AcPhe-tRNA. Hellenic Bioch. Biophys. Soc. (H.B.B.S.) Newsletter 30, Kallia-Raftopoulos, S., Synetos, D., Ottenheijm, H. C. J. and Coutsogeorgopoulos, C. (1994) Determination of the potency of sparsomycin analogs as inhibitors of ribosomal peptidyltransferase. Hellenic Biochem. Biophys. Soc. (H.B.B.S.) Newsletter 37, Frantziou, C.P., Alksne, L.E. and Synetos, D. (1995) Yeast ribosomal proteins S28 and S4 are involved in the control of translational accuracy. Hellenic Biochem. Biophys. Soc. (H.B.B.S.) Newsletter 38, Frantziou, C.P., Alksne, L.E. and Synetos, D. (1995) The aminoglycoside antibiotic paromomycin affects translational accuracy in a yeast cell-free system. Hellenic Biochem. Biophys. Soc. (H.B.B.S) Newsletter, 39, Dresios, J., Frantziou, C. P. and Synetos, D. (1996) Functional studies on ribosomes lacking protein L30 from mutant Saccharomyces cerevisiae. Hellenic Biochem. Biophys. Soc. (H. B. B. S.) Newsletter 41, Tzatsos, A., Nikitas, G., Frantziou, C. P., Dresios, J. and Synetos, D. (1998) Ribosomal proteins S4 and S28 are part of a translational accuracy center in the eukaryotic ribosome. Archives of Hellenic Medicine 15, Frantziou C. P. and Synetos D. (1999) Ribosomal protein S28 affects yeast ribosomal function and interacts with ribosomal protein S13 to control translational accuracy. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 45,

9 13. Dresios, J., Liebman, S. W. and Synetos, D. (1999) A mutation in the conserved sarcin/ricin domain of 25S yeast ribosomal RNA affects protein synthesis and decreases translational fidelity. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 45, Dresios, J., Panopoulos, P., Venieris, E., Georgatsou, E. and Synetos, D. (2000) Determination of peptidyltransferase activity of yeast mutants lacking ribosomal proteins L24, L39 or both. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 47, Panopoulos, P., Dresios, J., Choli-Papadopoulou, T. and Synetos, D. (2001) Ribosomal micro-protein L41 is dispensable but affects yeast ribosome activity. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 48, Panopoulos, P. and Synetos, D. (2002) Two non-essential yeast ribosomal proteins affect translocation and alter resistance to cycloheximide. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 49, Konstantinidis, T., Zouridakis, M. and Synetos, D. (2003) Mutations in yeast 18S rrna alter the structure and function of the ribosome including translational accuracy. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 50, Panopoulos, P., Dresios, J., Vamvakas, S. and Synetos, D. (2003) A translational accuracy switch in the yeast ribosome in the presence of two mutations, one in 26S rrna and the other in ribosomal protein S23. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 50, Konstantinidis, T., C., Belibasakis, G. and Synetos, D. (2004) A hygromycinresistant mutation in yeast 18S rrna and a substitution in 25S rrna modify translational fidelity. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 51, Konstantinidis, T. C., Tselika, S. and Synetos, D. (2006) Yeast ribosomal function is affected by an allosuppressor mutation in the SAL6 serine/threonine phosphatase. Hel. Soc. Biochem. & Mol. Biol. (HSBMB) Newsletter 53, Tselika, S., Apostolopoulou, F. and Synetos, D. (2007) Altered interaction of aminoglycoside antibiotics with ribosomes carrying mutations in the A-site of 18S rrna. 59 th Meeting of the Hellenic Society of Biochemistry and Molecular Biology, Athens, Greece, Newsletter 54, p. 286.

10 denotes corresponding author Communications in International Meetings 1. Schulster, D., Synetos, D. and Cooper, D.M.F. (1976) Characteristics of partially purified porcine corticotrophin releasing hormone, V Intern. Congress of Endocrinol, Hamburg, Abstract No Synetos, D. and Ballesta, J.P.G. (1985) Studies on rat liver ribosomes labelled by photoaffinity with the antibiotic pactamycin, VI Balkan Biochemical and Biophysical Days. Plovdiv, Bulgaria, April, Abstract No 4L5. 3. Synetos, D. and Coutsogeorgopoulos, C. (1986) An apparent catalytic rate constant for ribosomal peptidyltransferase, 17th FEBS Meeting Berlin (West), August, Biolog. Chem. Hoppe-Seyler 367 Suppl., Synetos, D. and Coutsogeorgopoulos, C. (1988) Binding of the donor substrate to the ribosomal P-site and reactivity towards puromycin, 14th Intern. Congress of Biochem., Prague, July, Abstract No FR Synetos, D., Dabeva, M. D. and Warner, J.R. (1992) The yeast ribosomal protein S7 is essential for cell growth and homologous to mammalian S4. 9th Balkan Biochemical and Biophysical Days, Thessaloniki, Greece, May, Abstract No Dinos, G., Synetos, D. and Coutsogeorgopoulos, C. (1992) The sparsomycin ribosomal complex: Determination of kinetic constants related to a proposed rearrangement. 9th Balkan Biochemical and Biophysical Days, Thessaloniki, Greece, May, Abstract No Dinos, G., Synetos, D. and Coutsogeorgopoulos, C. (1993) Kinetics of inhibition of peptide bond formation by spiramycin. 22nd FEBS Meeting, Stockholm, Sweden, 4-9 July, Abstract No. A Synetos, D., Frantziou, C. P. and Alksne, L.E. (1996) Evidence that ribosomal proteins S28 and S4 are part of a translational accuracy center in the yeast ribosome. 24th FEBS Meeting, Barcelona, Spain, 7-12 July. Abstract No PSOT Dresios, J. and Synetos, D. (1997) Studies on the function of ribosomes from S. cerevisiae lacking three ribosomal proteins of the 60S subunit. 11th Balkan Biochemical and Biophysical Days, Thessaloniki, Greece, May. Abstract No. 276.

11 10. Frantziou, C. P. and Synetos, D. (1997) Ribosomal protein S13 is part of a translational accuracy center in the yeast ribosome. 1st Balkan Biological Meeting, Thessaloniki, Greece, May, Proceedings pp Dresios, J., and Synetos, D. (1997) On the function of ribosomes from Saccharomyces cerevisiae lacking ribosomal proteins L30, L46 and L47. European Research Conferences: Molecular Biology of RNA. Giens, France September. Proceedings P24, p Dresios, J. and Synetos, D. (1998) Mutants lacking up to three ribosomal proteins provide insight into the properties of the Saccharomyces cerevisiae ribosome. 25 th FEBS Meeting, Copenhagen, Denmark, 5-10 July. 13. Panopoulos, P., Dresios, J., Liebman, S.W. and Synetos, D. (2004) A translational fidelity mutation in yeast 25S ribosomal RNA affects peptidyltransferase activity and translocation. 29 th FEBS Meeting, Warsaw, Poland 26 June-1 July. Abstract No: Konstantinidis, T. C., Patsoukis, N., Georgiou, C. D. and Synetos, D. (2005) Mutations in yeast 18S rrna affect translational fidelity and oxidative stress. 30 th FEBS Meeting and 9 th IUBMB Meeting, Budapest, Hungary 2-7 July Tselika, S. and Synetos, D. (2006) Activity differences of two aminoglycoside antibiotics in yeast ribosomal function uncovered by mutations in 18S rrna. 31 st FEBS Meeting, Constantinople, Turkey, June. The FEBS Journal 273, Supplement 1, p Synetos, D., Konstantinidis, T. C., Tselika, S., Panopoulos, P. and Dresios, J. (2007) Involvement of 18S and 25S rrna in translational fidelity and catalytic activity of yeast ribosomes. Experimental Biology, American Society of Biochemistry and Molecular Biology (ASBMB), Washington, DC, USA (Poster 5128). 17. Konstantinidis, T. C., Tselika, S. and Synetos, D. (2008) Mutations in yeast 18S rrna affect translational fidelity, efficiency of protein synthesis and resistance towards aminoglycoside antibiotics. 33rd FEBS Congress and 11th IUBMB Conference, Athens, Greece, 28 June-3 July Konstantinidis, T. C., Derkatch, I. L., Vincent, A., Liebman, S. W. and Synetos, D. (2010) Yeast ribosomal function is affected by two mutations, one in an extraribosomal phosphatase and another in a 40S subunitassociated gene. International Workshop Ribosomes 2010 (Rodnina, M. and Green, R., organizers) Orvieto, Italy, 3-7 May 2010 (Poster 79).

12 Communications in National Meetings 1.. Συνετός και H. O. Voorma (1984) Καθαρισμός και προκαταρκτικός χαρακτηρισμός των eif2 και erf από ήπαρ επίμυος. 24 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Ιωάννινα, 5-6 Μαΐου. 2.. Συνετός (1985) Σήμανση φωτοσυγγενείας ριβοσωμάτων ήπατος επίμυος με τη βοήθεια του αντιβιοτικού πακταμυκίνη. 25 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Αθήνα, Ιανουαρίου. 3.. Συνετός και Χ. Κουτσογεωργόπουλος (1986) Η πρόσδεση του εναρκτήριου f-met-trna στο ριβόσωμα ενεργοποιεί την πεπτιδυλοτρανσφεράση. 27 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Πάτρα, Απριλίου. 4.. Συνετός και Χ. Κουτσογεωργόπουλος (1987) Η επίδραση των ιόντων μαγνησίου επί της ενεργότητας της πεπτιδυλοτρανσφεράσης.. 30 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Αθήνα, Νοεμβρίου. 5.. Συνετός,. Α. Θεοχάρης,. Λ. Καλπαξής,. ραΐνας και Χ. Κουτσογεωργόπουλος (1988) Κινητική της αναστολής της ριβοσωματικής πεπτιδυλοτρανσφεράσης : Σύγκριση μεταξύ ριβοσωμάτων προσροφημένων επί νιτρικής κυτταρίνης και ριβοσωμάτων εν διαλύματι. 31 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας, Αθήνα, Νοεμβρίου. 6. Γ. Ντίνος,. Συνετός και Χ. Κουτσογεωργόπουλος (1990) Το αντιβιοτικό σπιραμυκίνη σχηματίζει σφικτό σύμπλοκο με το 70S ριβόσωμα και το Ac-PhetRNA. 33 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Πάτρα, 1-2 Ιουνίου. 7. Σ. Καλλία-Ραυτοπούλου,. Συνετός, H. C. J. Ottenheijm και Χ. Κουτσογεωργόπουλος (1994) Προσδιορισμός της ισχύος αναλόγων σπαρσομυκίνης ως αναστολέων της ριβοσωματικής πεπτιδυλοτρανσφεράσης. 41 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Αθήνα, Ιανουαρίου.

13 8. Χ. Π. Φραντζιού, L. E. Alksne και. Συνετός (1995) Οι ριβοσωματικές πρωτεΐνες S28 και S4 της ζύμης συμμετέχουν στη ρύθμιση της μεταφραστικής ακρίβειας. 42 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Αθήνα, Ιανουαρίου. 9. Χ. Π. Φραντζιού, L. E. Alksne και. Συνετός (1995) Το αμινογλυκοζιτικό αντιβιοτικό παρομομυκίνη επηρεάζει τη μεταφραστική ακρίβεια σε σύστημα ελεύθερο κυττάρων ζύμης. 43 η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας. Ιωάννινα, Μαΐου. 10. Ι. ρέσιος, Χ. Π. Φραντζιού και. Συνετός (1996) Λειτουργικές μελέτες επί μεταλλαγμένων ριβοσωμάτων του S. cerevisiae από τα οποία λείπει η πρωτεΐνη L η Πανελλήνια Συνεδρία της Ελληνικής Βιοχημικής και Βιοφυσικής Εταιρείας, Πάτρα, Μαΐου. 11. Γ. Νικήτας, Α. Τζάτσος, Χ. Π. Φραντζιού, Ι. ρέσιος και. Συνετός (1997) Οι ριβοσωματικές πρωτεΐνες S4 και S28 συμμετέχουν στο κέντρο ακρίβειας της μετάφρασης του ευκαρυωτικού ριβοσώματος. 3 ο Επιστημονικό Συνέδριο Φοιτητών Ιατρικής Ελλάδος, Ηράκλειο Κρήτης, Απριλίου. 12. Χ. Π. Φραντζιού και. Συνετός (1999) Η ριβοσωματική πρωτεΐνη S28 επηρεάζει τη λειτουργία του ριβοσώματος της ζύμης και αλληλεπιδρά με την ριβοσωματική πρωτεΐνη S13 για τη ρύθμιση της μεταφραστικής ακρίβειας. 50 ο Πανελλήνιο Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Αθήνα, Ιανουαρίου. 13. Ι. ρέσιος, S. W. Liebman και. Συνετός (1999) Μια μετάλλαξη στη διατηρημένη περιοχή σαρκίνης/ρισίνης του 25S rrna της ζύμης επιδρά στην πρωτεϊνοσύνθεση και ελαττώνει την πιστότητα της μετάφρασης. 50 ο Πανελλήνιο Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Αθήνα, Ιανουαρίου. 14. Ι. ρέσιος, Π. Πανόπουλος, Ε. Βενιέρης, Ε. Γεωργάτσου και. Συνετός (2000) Ενεργότητα πεπτιδυλοτρανσφεράσης μεταλλαγμένων στελεχών ζύμης από τα οποία απουσιάζουν οι ριβοσωματικές πρωτεΐνες L24, L39 ή και οι δυο. 52 ο Πανελλήνιο Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Θεσσαλονίκη, Νοεμβρίου. 15. Vamvakas, S., Dresios, J. and Synetos, D. (2001) A combination of mutations in 25S yeast rrna and in ribosomal protein S23 affects

14 translational fidelity and polyphenylalanine synthesis. Proceedings 23 rd Meeting Hel. Soc. Biol. Sci., Chios, May, Π. Πανόπουλος, Ι. ρέσιος, Θ. Χολή-Παπαδοπούλου και. Συνετός (2001) Η ριβοσωματική μικρο-πρωτεΐνη L41 είναι μη απαραίτητη αλλά επηρεάζει την ενεργότητα του ριβοσώματος της ζύμης. 53 ο Πανελλήνιο Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Αθήνα, εκεμβρίου. 17. Konstantinidis, T., Panopoulos, P. and Synetos, D. (2002) Mutations in yeast 18S rrna alter the function of the ribosomal accuracy center and the resistance to antibiotic paromomycin. Proceedings 24 rd Meeting Hel. Soc. Biol. Sci., Eretria, May, Bouzianis, P, Panopoulos, P. and Synetos, D. (2002) Effect of mutations in helix 27 of yeast 18SrRNA on translational fidelity and ribosomal peptidyltransferase activity. Proceedings 24 rd Meeting Hel. Soc. Biol. Sci., Eretria, May, Π. Πανόπουλος και. Συνετός (2002) υο μη απαραίτητες ριβοσωματικές πρωτεΐνες της ζύμης επηρεάζουν τη μετατόπιση και μεταβάλλουν την ανθεκτικότητα προς το κυκλοεξιμίδιο. 54 ο Πανελλήνιο Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Ιωάννινα, Οκτωβρίου. 20. Zouridakis, M., Konstantinidis, T. and Synetos, D. (2003) Three site-directed mutations in yeast 18S rrna affect translational fidelity and resistance to aminoglycoside antibiotics. Proceedings of the 25 th Scientific Conference, Hellenic Society for Biological Sciences, Mytilini, Greece, 29 May-1 June, pp Συνετός,. (2003) Μελέτη της λειτουργίας του ριβοσώματος με τη βοήθεια μεταλλάξεων που επηρεάζουν την πρωτεινοσύνθεση και μεταβάλλουν την ανθεκτικότητα των κυττάρων έναντι ορισμένων αντιβιοτικών, 2 η ιημερίδα Ερευνητικών ραστηριοτήτων Τμήματος Ιατρικής Παν/μίου Πατρών, Πάτρα, 6-7 Ιουνίου. 22. Θ. Κωνσταντινίδης, Μ. Ζουριδάκης και. Συνετός (2003) Μεταλλάξεις στο 18S rrna της ζύμης μεταβάλλουν τη δομή και λειτουργία του ριβοσώματος συμπεριλαμβανομένης της μεταφραστικής πιστότητας. 55 ο Πανελλήνιο

15 Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Αθήνα, Νοεμβρίου. 23. Π. Πανόπουλος, Ι. ρέσιος, Σ. Βαμβακάς και. Συνετός (2003) Μεταβολή της μεταφραστικής πιστότητας των ριβοσωμάτων ζύμης παρουσία δύο μεταλλάξεων, μιας στο 26S rrna και της άλλης στη ριβοσωματική πρωτεΐνη S ο Πανελλήνιο Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Αθήνα, Νοεμβρίου. 24. Tselika, S and Synetos, D. (2004) The role of a mutation in the 18S rrna binding site of paromomycin in translational fidelity. Proceedings of the 26 th Scientific Conference, Hellenic Society for Biological Sciences, Volos, Greece, May, pp Konstantinidis, T. C. and Synetos, D. (2004) An allosuppressor mutation in yeast phosphatase SAL6 affects translational accuracy. Proceedings of the 26 th Scientific Conference, Hellenic Society for Biological Sciences, Volos, Greece, May, pp Θ. Κωνσταντινίδης, Γ. Μπελιμπασάκης και. Συνετός (2004) Μια ανθεκτική προς την υγρομυκίνη μετάλλαξη στο 18S rrna της ζύμης και μια υποκατάσταση στο 25S rrna μεταβάλλουν τη μεταφραστική πιστότητα. 56 ο Πανελλήνιο Συνέδριο της Ελληνικής Εταιρείας Βιοχημείας και Μοριακής Βιολογίας, Λάρισα, Νοεμβρίου. 27. Konstantinidis, T. C., Goudevenou, K. and Synetos, D. (2005) Two nucleotide substitutions in loop 530 of yeast 18S rrna modify ribosomal function in opposite directions. Proceedings of the 27 th Scientific Conference, Hellenic Society for Biological Sciences, Nafplion, Greece, May, pp Panopoulos, P., Dinos, G., Nierhaus, K. H. and Synetos, D. (2005) Effect of edeine and pactamycin on the fidelity of translation and on P- and A-site trna binding in yeast ribosomes. Proceedings of the 27 th Scientific Conference, Hellenic Society for Biological Sciences, Nafplion, Greece, May, Konstantinidis, T. C., Panopoulos, P., Tselika, S. and Synetos, D. (2005) Effect of mutations in yeast 18S rrna on translational fidelity using synthetic mrna and on the translocation of protein synthesis. Proceedings of the 27 th

16 Scientific Conference, Hellenic Society for Biological Sciences, Nafplion, Greece, May, Konstantinidis, T. C., Zouridakis, M., Patsoukis, N., Zervoudakis, G. Georgiou, C. D. and Synetos, D. (2006) Translational accuracy mutations influence the oxidative status of yeast cells. Proceedings of the 28 th Scientific Conference, Hellenic Society for Biological Sciences, Ioannina, Greece, May, Tselika, S., Konstantinidis, T. C. and Synetos, D. (2006) A mutation in the decoding region of yeast 18S rrna affects translation and confers resistance to aminoglycoside antibiotics. Proceedings of the 28 th Scientific Conference, Hellenic Society for Biological Sciences, Ioannina, Greece, May, Bougas, A. Shaked, S., Fridman, E. and Synetos, D. (2011) Yeast hybrids possessing a ribosomal heterosis trait locus show altered translational fidelity and increased sensitivity to the aminoglycoside antibiotic paromomycin. 62 nd Hellenic Society of Biochemistry and Molecular Biology (HSBMB) Meeting, Athens, Greece, 9-11 December 2011, Abstract P31, p Ntouska, M. and Synetos, D. (2012) Functional studies in the eukaryotic ribosome under the influence of the antibiotics edeine and pactamycin. 38 th Panhellenic Medical Conference, Athens Hilton, Greece, May 2012, Communication 47, p Bougas, A., Fridman, E. and Synetos, D. (2012) The role of ribosomal protein L39 as a possible quantitative trait locus in yeast heterosis. 63rd Hellenic Society of Biochemistry and Molecular Biology (HSBMB) Meeting, Herakleion Crete, Greece, 9-11November 2012, Abstract Bougas, A., Fridman, E. and Synetos, D. (2013) Catalytic properties of ribosomes from heterotic yeast strains exhibiting translational infidelity. 64th Hellenic Society of Biochemistry and Molecular Biology (HSBMB), Athens, Greece, 6-8 December 2013 (to be presented).