Colchicum kotschyi Boiss

Transcript

1 Journal of Horticultural Science Vol. 25, No. 4, Winter 202, P ISSN: ( ) : Colchicum kotschyi Boiss * /2/5: 90/7/5: 6 (Colchicaceae ) Colchicum kotschyi Boiss : : ) Colchicum kotschyi Boiss ( 5) ( : /553 mg/g 28/ /407 mg 0/264 mg/g 23 7/04 mg Colchicum kotschyi Boiss :.(4) (20).(22) (2 7). (8).(22) 8- Colchicine (24) 00 6.(2) ( mortezaalirezaie@yahoo.com : -*) Colchicum L. 7- Colchicaceae

2 ( ) C.kotschyi (6)..(4). 2 /58 0/5 0/ : 0/34 0/02 0/ /0 0/00. ( 5 ). 2- Knop... :.(9).(4 0) 5/2 (5).(5)...(0) (4 3). 75:00:75.. C. speciusum.(25) knop. C.kotschyi - Catharanthus roseus

3 Colchicum kotschyi Bioss - (m) (mm) C ,4 260,6 4,2 ( ) ,5 ( ) HPLC. : HPLC degasser Knauer HPLC HPLC pump K-00 knauer.dynamic Auto smaller knauer mixing chamber 2. (23:77) 50 2 Bondapck C8 (3.9*300) mg. 0 ml. 20 μg/ml HPLC.( ). 2.. MSTAT-C. Excel - High Performance Liquid Chromatography (HPLC) 2- Flow rate - 2 ( ) Ca K P N (2) ml. 250 ml ml 35 5 ml ml 0 ml. 0ml 30 ml 3 ).( ( ) HPLC 5 ml.

4 ( ) 428-0/ ) (3 ) ( 28/4. 23.(3 ) cm 3/8 2/256.(3 ) ) /95 /65 ( ) ( /95 8/506

5 (g) 42/53 b 44/96 b C. kotschyi - 3 (mg) 0/48 c /408 b ( mg/g) 0/246 c 0/553a (cm) 2/244a 2/000a (cm) 3/57a 3/633a (g) /950 b 2/533a (g) 8/506 b 8/933 b 58/23a 0/899 b 0/304 b 2/256a 3/800a 2/950a /65a 8/530 0/2028 0/0226 0/398 0/886 0/569 2/439 LSD. % : : : : : :..(3) 240.(27) 0/553 mg/g mg/g /408 mg. 0/48 mg 0/246.(3 5 ). 58/23 42/53 4/77 g. 9/777 g..(4 ) (4) 00:75 : Allium sativum L.

6 ( ) : : :. - 5.(27).. ( /5 2/75). 2.() (3) 2- Ginseng (6). /5 / /5 00 ( )...(3).(30) - Gloriosa superba

7 43....(28 20).(29 8)... C. kotschyi /407 mg 7/04 ( ) ) ) (.(6 ) ( (7). 5 : 5 :70 (23). (400 ppm) (200 ppm) (20:0:20) Sanguinaria canadensis : : :. - Chelidonium majus L.

8 ( ) Abdolzadeh A., Hosseinian F., Aghdasi M. and Sadgipoor H Effects of nitrogen sources and levels on growth and alkaloid content of Perivinkle. Asian Journal of Plant Science, 5: Alali F., El-Alali A., Tawaha K.H. and Al Elimat T Seasonal variation of colchicine content in Colchicum brachyphyllum and Colchicum tunicatum (Colchicaceae). Natural Product Research, 20: Al-Fayyad M., Alali F., Alkofahi A. and Tell A Determination of colchicine content in Colchicum hierosolymitanum and Colchicum tunicatum under cultivation. Natural Product Letters, 6: Al-Fayyad M., Alali F. and Al-Tell A Effect of NPK fertilizer levels on morphological characteristics and productivity of Colchicum hierosolymitanum and Colchicum tunicatum, Journal of herbs, spices & medicinal plants, 4: Babakhanyan M.A. 99. Introduction of Catharanthus roseus G. Don in open-air hydroponics in the Ararat Valley in Armenia. Herba Hungarica, 30: Bharathi P. and Philomina D Effect of nutritional factors and precursors on formation of colchicine in Gloriosa superba in vitro. Research in Biotechnology, : Boye O. and Brossi A Tropolonic colchicum alkaloids and allo congeners. The Alkaloids. Academic Press Inc, New York. 8- Cerquaglia C., Diaco M., Nucera G., La Regina M., Montalto M. and Manna R Pharmacological and clinical basis of treatment of Familial Mediterranean Fever (FMF) with colchicine or analogues. Current Drug Targets - Inflammation & Allergy, 4: Dorais M., Papadopoulos A.P., Luo X., Leonhart S., Gosselin A., Pedneault K., Angers P. and Gaudreau L Soilless greenhouse production of medicinal plants in north eastern Canada. Acta Horticulture, 554: Hayden A.L Aeroponic and Hydroponic Systems for Medicinal Herb, Rhizome, and Root Crops. Horticultural Science, 4: Kaplan M.M., Schmid C., Provenzale D., Sharma A., Dickstein G. and Mckusik A A prospective trial of colchicine and methotrexate in the treatment of primary biliary cirrhosis. Gastroenterology, 7: Katzung G.B Basic and Clinical Pharmacology, 9th edi. The McGraw- Hill companies Inc USA, 33: Kilgori M.J., Magagi M.D. and Yakubu A.I Productivity of two Garlic (Allium sativum L.) cultivars as affected different levels of nitrogen and phosphorous fertilizers in Sokoto, Nigeria. American-Eurasian Journal of. Agricultural & Environmental Sciences, 2: Komjatayova H., Frankova L., Boka K. and Psenak M Botanical and developmental aspects of Colchicum autumnale L. (autumn crocus) (in Slovak). Acta Facultatis Pharmaceuticae Universitatis Comenianae, 40: Limon J.N Valerian (Valeriana officinalis L.) in soilless culture. Acta Horticulture, 32: Manukyan A.E The development of biotechnologies of celandine and catmint in outside hydroponics conditions, PhD diss. summary, 2. National Academy of Sciences of the Republic of Armenia (in Armenian). 7- Manukyan A.E Optimum nutrition for biosynthesis of pharmaceutical compounds in Celandine and Catmint under outside hydroponic conditions', Journal of Plant Nutrition, 28: 5, Marino P.C., Eisenberg R.M. and Cornell H.V Influence of sunlight and soil nutrients on clonal growth and sexual reproduction of the understory perennial herb Sanguinaria Canadensis L. Bull. Torrey Botanical Society, 24: Miller J.S. and Feeny P Effects of benzylisoquinoline alkaloids on the larvae of polyphagous Lepidoptera. Oecologia, 58: Pelletier P.J. et Caventou J.B Examen chimique de plusieurs vegetaux de la famille des colchicees, et du principe actif qu'ils renferment [Cevadille (Veratrum sabadilla)]; hellebor blanc (Veratrum album); colchique commun (Colchicum autumnale). Annal Review of Physical Chemistry, 4: Persson K Liliaceae III. Subfam. I. Wurmbaeoideae. In: Rechinger K. H. (ed.), Flora Iranica. Akademische Druck- u. Verlagsanstalt, Graz, 70: Poutaraud A. and Champay N Meadow saffron (Colchicum autumnale L.) a medical plant to domesticate. Revue Suisse- Agriculture, 27: Salmore A.K. and Hunter M.D Environmental and genotypic influences on isoquinoline alkaloid content in Sanguinaria Canadensis. Journal of Chemical Ecology, 27: Santavy F., Dvorackova S., Simanek V. and Potesilova H Isolation and identification of alkaloids of the subfamily Wurmbaeiodeae. Medical Faculty of the University Palacky, Olomouc, Czech Republic, Sargsyan K.H.L Alternative approach to colchicines production applying open air hydroponics cultivation. p International Conference & Daad Alumni Seminar Biotechnolog y and Health, Apr Yerevan, Armenia. 26- Schmeller T., Latz-Bruning B. and Wink M Biochemical activites of berberine, palmatine, and sanguinarine

9 mediating chemical defense against microorganisms and herbivores. Phytochemistry, 44: Sreevalli Y., Kulkarni R.N., Baskaran K. and Chandrashekara R.S Increasing the content of leaf and root alkaloids of high alkaloid content mutants of periwinkle through nitrogen fertilization. Industrial Crops and Products, 9: Tran T.L.M., Bui C.T., Bourgaurd F. and Gontier E Production of the secondary metabolite with hydroponic culture. Journal of Agricultural Science and Technology, 2: Tuomi J., Niemela P. and Haukioja E Nutrient stress: An explanation for plant anti-herbivore responses to defoliation. Oecologia, 6: Waller G.R. and Nowacki E.K Alkaloid Biology and Metabolism in Plants, Plenum Press, New York. USA, pp: Zhong J. and Wang S Effects of nitrogen sources on the production of ginseng and polysaccharide by cell cultures of panax quinuefolium. Process Biochemistry, 33: