Data-Driven Discovery of Extravasation Pathway in Circulating Tumor Cells

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1 Data-Driven Discovery of Extravasation Pathway in Circulating Tumor Cells Yadavalli S. 1, Jayaram S. 1,2, Manda SS. 1,3, Madugundu AK. 1,3, Nayakanti DS. 1, Tuan TZ. 6, Bhat R. 4, Rangarajan A. 4, Chatterjee A. 1,5, Gowda H. 1,5, Thiery JP. 6,7,8, Kumar P 1. 1 Institute of Bioinformatics, International Technology Park, Whitefield, Bangalore, , India. 2 Manipal University, Madhav Nagar, Manipal, , India. 3 Center for Bioinformatics, Pondicherry University, Puducherry , India. 4 Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, , India. 5 YU-IOB Center for Systems Biology and Molecular Medicine, Yenepoya University, Mangalore, , India. 6 Cancer Science Institute of Singapore, National University of Singapore, Centre for Translational Medicine NUS Yong Loo Lin School of Medicine, Singapore , Singapore. 7 Comprehensive Cancer Center, Institut Gustave Roussy, 114 Rue Edouard Vaillant, Villejuif, France; CNRS UMR 7057, Matter and Complex Systems, Université Paris Diderot, 10 rue Alice Domon et Léonie Duquet Paris, France. 8 Department of Biochemistry, National University of Singapore, Singapore , Singapore. Correspondence and requests for materials should be addressed to Kumar P. ( prashant@ibioinformatics.org) 1

2 Supplementary Information As shown in the supplementary figure 1a, the total number of papers that resulted from PubMed search with keyword circulating tumor cells signaling is 1,073 (blue portion of the Venn diagram; publications). This includes the papers that were common to results from keyword searches circulating tumor cells signaling and cancer (648); circulating tumor cells signaling and epithelial mesenchymal transition (2); and results common to all three keyword searches (45). The papers unique to the last two searches were excluded as they were not relevant. After abstract-level screening of these 1,073 papers, 153 were narrowed down for in-depth screening. Similarly, as shown in the supplementary figure 1b, the total number of papers that resulted from PubMed search with keyword circulating tumor cells gene expression is 2,169 (blue portion of the Venn diagram; ,043 publications). This includes the papers that were common to results from keyword searches circulating tumor cells genes (214); circulating tumor cells expression (1,043); and results common to all three keyword searches (424). The papers unique to the last two searches were excluded due to non-relevance and large numbers. After abstract-level screening of these 2,169 papers, 78 were narrowed down for in-depth screening. Out of these 231 papers (153+78), 98 papers were found to have gene expression data relevant to CTCs from which the gene list was compiled for our analysis. Of these, 93 papers were smallscale qualitative and semi-quantitative experiments that reported expression of a few genes in CTCs. The remaining five papers were based on large-scale transcriptomic experiments (RNA- Seq and Microarray) and reported differential expression of hundreds of genes in CTCs. The final gene list for our pathway analysis comprises mostly of genes from these five papers (7,209 out 7,572 total numbers of genes). These numbers are reported as CTC_ALL (7,572 genes from all 98 papers) out of which 7,209 (CTC_FC) were taken from the five large-scale transcriptomics experiment papers (Supplementary Table S1 and Supplementary Table S2, respectively). The reference information (PubMed IDs; PMIDs) for the 98 papers are listed in Supplementary Table S5. 2

3 Supplementary Figures Supplementary Figure 1: Venn diagrams showing overlap between research articles when searched with keywords in Pubmed. (a) PubMed results with keywords containing circulating tumor cells signaling ; (b) PubMed results with keywords containing circulating tumor cells gene expression ; (c) Venn diagram showing number of differentially expressed genes in circulating tumor cells taken from semi-quantitative and qualitative experiments shown in CTC_ALL (n=7,572). Subset of differentially expressed genes from semi-quantitative experiments from five cancer studies is shown as CTC_FC (n=7,209). 3

4 Supplementary Figure 2: Venn diagram showing the number of genes differentially expressed in CTCs in the five cancers. Number of genes overexpressed in CTCs when compared to primary tumors (a), peripheral blood mononuclear cells (PBMCs) (b), normal tissue (c). Number of genes downregulated in CTCs when compared to primary tumors (d), PBMCs (e), normal tissue (f). 4

5 Supplementary Figure 3: Swarm plot shows the distribution of differentially expressed epithelial (green) and mesenchymal (blue) genes across the five cancer datasets. The genes are further segregated on their expression as epithelial or mesenchymal markers based on published literature. PRCTC1 denotes prostate CTCs gene expression when compared to primary tumors; CRCTC1 colorectal CTCs when compared to PBMCs; MNCTC1 melanoma CTCs/melanoma cell lines; MNCTC2 melanoma CTCs/primary melanocytes; PACTC1 pancreatic CTCs/nontumoral pancreatic tissue; PACTC2 pancreatic CTCs/PBMCs; PACTC3 pancreatic CTCs/primary tumors; BRCTC1 breast CTCs/breast epithelium; BRCTC2 breast CTCs/PBMCs; BRCTC3 breast CTCs/primary tumors. 5

6 Supplementary Figure 4: Comparison of EMT scores of CTC gene expression data for the five cancers with a) EMT scores of tumors and b) EMT scores of cancer cell lines. The CTC dataset labels are highlighted in boxes. 6

7 Supplementary Tables Supplementary Table S1: CTC_ALL: Differentially expressed genes in circulating tumor cells when compared with primary tumors, PBMCs, normal tissue and tumor cell lines. Expression data for a total number of 7,572 genes were obtained from reported literature of semiquantitative and qualitative experiments. Supplementary Table S2: CTC_FC: Differentially expressed genes in circulating tumor cells when compared with primary tumors, PBMCs, normal tissue and tumor cell lines. Data for a total of 7,209 genes were obtained from reported literature of transcriptomics experiments from five different cancer studies of CTCs. Supplementary Table S3: CTC isolation and purity confirmation techniques used by the five high-throughput experiments from which differentially expressed genes with fold change values (CTC_FC) was compiled for data analysis in our study. Supplementary Table S4: List of molecular interactions reported in the literature (PubMed IDs included) and depicted in the leukocyte/ctc extravasation pathway. Supplementary Table S5: PubMed IDs for 98 papers from which a gene list was compiled for molecular characterization of CTCs. This list includes the five large-scale transcriptomics experiment papers from which differentially expressed genes with fold change values were obtained (highlighted in yellow color). Supplementary Table S6: EMT scores computed from transcriptome datasets of CTCs in five cancers GSE18670, GSE31023, GSE38495, GSE45965, and GSE The pre-processed data GSE45965 was also used. 7

8 Yadavalli et al., Data-Driven Discovery of Extravasation Pathway in Circulating Tumor Cells Supplementary Table S1: CTC_ALL: Differentially expressed genes in circulating tumor cells when compared to primary tumors, PBMC, normal tissue and tumor cell lines. Expression data for a total number of 7,572 genes were obtained from reported literature of semi-quantitative and qualitative experiments. Gene Symbol Gene ID Gene Description Fold change (log2) Arbitrary fold change (for CTC_ALL analysis) 1 RPS4Y ribosomal protein S4, Y-linked 1 [Source:HGNC Symbol;Acc:HGNC:10425] COX7B cytochrome c oxidase subunit 7B2 [Source:HGNC Symbol;Acc:HGNC:24381] GTSF gametocyte specific factor 1 [Source:HGNC Symbol;Acc:HGNC:26565] SMUG single-strand-selective monofunctional uracil-dna glycosylase 1 [Source:HGNC Symbol;Acc:HGNC:17148] PAGE PAGE family member 5 [Source:HGNC Symbol;Acc:HGNC:29992] TUBB tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HGNC:16257] PPBP 5473 pro-platelet basic protein [Source:HGNC Symbol;Acc:HGNC:9240] TMEFF transmembrane protein with EGF like and two follistatin like domains 2 [Source:HGNC Symbol;Acc:HGNC:11867] PF platelet factor 4 [Source:HGNC Symbol;Acc:HGNC:8861] ANKHD ankyrin repeat and KH domain containing 1 [Source:HGNC Symbol;Acc:HGNC:24714] ANKHD1-EIF4EBP ANKHD1-EIF4EBP3 readthrough [Source:HGNC Symbol;Acc:HGNC:33530] MLEC 9761 malectin [Source:HGNC Symbol;Acc:HGNC:28973] S100A S100 calcium binding protein A12 [Source:HGNC Symbol;Acc:HGNC:10489] FCN ficolin 1 [Source:HGNC Symbol;Acc:HGNC:3623] ACRBP acrosin binding protein [Source:HGNC Symbol;Acc:HGNC:17195] CCNB1 891 cyclin B1 [Source:HGNC Symbol;Acc:HGNC:1579] NRGN 4900 neurogranin [Source:HGNC Symbol;Acc:HGNC:8000] SREK splicing regulatory glutamic acid and lysine rich protein 1 [Source:HGNC Symbol;Acc:HGNC:17882] CENPM centromere protein M [Source:HGNC Symbol;Acc:HGNC:18352] ANLN anillin actin binding protein [Source:HGNC Symbol;Acc:HGNC:14082] KIAA KIAA0895 [Source:HGNC Symbol;Acc:HGNC:22206] LYZ 4069 lysozyme [Source:HGNC Symbol;Acc:HGNC:6740] CKAP cytoskeleton-associated protein 4 [Source:HGNC Symbol;Acc:HGNC:16991] ITGA2B 3674 integrin subunit alpha 2b [Source:HGNC Symbol;Acc:HGNC:6138] TSG tumor susceptibility 101 [Source:HGNC Symbol;Acc:HGNC:15971] SRSF serine and arginine rich splicing factor 11 [Source:HGNC Symbol;Acc:HGNC:10782] SNX sorting nexin 10 [Source:HGNC Symbol;Acc:HGNC:14974] BMX 660 BMX non-receptor tyrosine kinase [Source:HGNC Symbol;Acc:HGNC:1079] HIST1H3H 8357 histone cluster 1, H3h [Source:HGNC Symbol;Acc:HGNC:4775] ECI enoyl-coa delta isomerase 2 [Source:HGNC Symbol;Acc:HGNC:14601] RUVBL RuvB like AAA ATPase 1 [Source:HGNC Symbol;Acc:HGNC:10474] MRPL mitochondrial ribosomal protein L46 [Source:HGNC Symbol;Acc:HGNC:1192] MAF MAF1 homolog, negative regulator of RNA polymerase III [Source:HGNC Symbol;Acc:HGNC:24966] PF4V platelet factor 4 variant 1 [Source:HGNC Symbol;Acc:HGNC:8862] YY1AP YY1 associated protein 1 [Source:HGNC Symbol;Acc:HGNC:30935] TIMMDC translocase of inner mitochondrial membrane domain containing 1 [Source:HGNC Symbol;Acc:HGNC:1321] LYAR Ly1 antibody reactive [Source:HGNC Symbol;Acc:HGNC:26021] TAL T-cell acute lymphocytic leukemia 1 [Source:HGNC Symbol;Acc:HGNC:11556] SF3B splicing factor 3b subunit 6 [Source:HGNC Symbol;Acc:HGNC:30096] BUD BUD31 homolog [Source:HGNC Symbol;Acc:HGNC:29629] RPL10L ribosomal protein L10 like [Source:HGNC Symbol;Acc:HGNC:17976] MGAM 8972 maltase-glucoamylase [Source:HGNC Symbol;Acc:HGNC:7043] ALOX arachidonate 12-lipoxygenase, 12S type [Source:HGNC Symbol;Acc:HGNC:429] CCNB cyclin B2 [Source:HGNC Symbol;Acc:HGNC:1580] CYFIP cytoplasmic FMR1 interacting protein 2 [Source:HGNC Symbol;Acc:HGNC:13760] TREML triggering receptor expressed on myeloid cells like 1 [Source:HGNC Symbol;Acc:HGNC:20434] DNAJA DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885] HBG hemoglobin subunit gamma 1 [Source:HGNC Symbol;Acc:HGNC:4831] FUNDC FUN14 domain containing 2 [Source:HGNC Symbol;Acc:HGNC:24925] RGS regulator of G-protein signaling 18 [Source:HGNC Symbol;Acc:HGNC:14261] KRTAP keratin associated protein 19-1 [Source:HGNC Symbol;Acc:HGNC:18936] LPXN 9404 leupaxin [Source:HGNC Symbol;Acc:HGNC:14061] SLC35B solute carrier family 35 member B1 [Source:HGNC Symbol;Acc:HGNC:20798] USP ubiquitin specific peptidase 15 [Source:HGNC Symbol;Acc:HGNC:12613] NAMPT nicotinamide phosphoribosyltransferase [Source:HGNC Symbol;Acc:HGNC:30092] AHSP alpha hemoglobin stabilizing protein [Source:HGNC Symbol;Acc:HGNC:18075] EWSR EWS RNA binding protein 1 [Source:HGNC Symbol;Acc:HGNC:3508] CELF CUGBP, Elav-like family member 1 [Source:HGNC Symbol;Acc:HGNC:2549] IFT intraflagellar transport 57 [Source:HGNC Symbol;Acc:HGNC:17367] SLA Src-like-adaptor 2 [Source:HGNC Symbol;Acc:HGNC:17329] SERPINA3 12 serpin family A member 3 [Source:HGNC Symbol;Acc:HGNC:16] HMGN high mobility group nucleosomal binding domain 4 [Source:HGNC Symbol;Acc:HGNC:4989] TPP tripeptidyl peptidase 2 [Source:HGNC Symbol;Acc:HGNC:12016] NLRC NLR family CARD domain containing 5 [Source:HGNC Symbol;Acc:HGNC:29933] GNAZ 2781 G protein subunit alpha z [Source:HGNC Symbol;Acc:HGNC:4395] SERPINB serpin family B member 6 [Source:HGNC Symbol;Acc:HGNC:8950] CDKN cyclin dependent kinase inhibitor 3 [Source:HGNC Symbol;Acc:HGNC:1791] PNN 5411 pinin, desmosome associated protein [Source:HGNC Symbol;Acc:HGNC:9162] TSTD thiosulfate sulfurtransferase like domain containing 1 [Source:HGNC Symbol;Acc:HGNC:35410] CST cystatin F [Source:HGNC Symbol;Acc:HGNC:2479] 5.4 2

9 71 IGF2BP insulin like growth factor 2 mrna binding protein 3 [Source:HGNC Symbol;Acc:HGNC:28868] CIAPIN cytokine induced apoptosis inhibitor 1 [Source:HGNC Symbol;Acc:HGNC:28050] RAB RAB37, member RAS oncogene family [Source:HGNC Symbol;Acc:HGNC:30268] SDPR 8436 serum deprivation response [Source:HGNC Symbol;Acc:HGNC:10690] AP3D adaptor related protein complex 3 delta 1 subunit [Source:HGNC Symbol;Acc:HGNC:568] MSL male specific lethal 1 homolog [Source:HGNC Symbol;Acc:HGNC:27905] PSMB proteasome subunit beta 8 [Source:HGNC Symbol;Acc:HGNC:9545] HIST1H2AG 8969 histone cluster 1, H2ag [Source:HGNC Symbol;Acc:HGNC:4737] HMMR 3161 hyaluronan mediated motility receptor [Source:HGNC Symbol;Acc:HGNC:5012] IL2RB 3560 interleukin 2 receptor subunit beta [Source:HGNC Symbol;Acc:HGNC:6009] RBM RNA binding motif protein 18 [Source:HGNC Symbol;Acc:HGNC:28413] BUB1 699 BUB1 mitotic checkpoint serine/threonine kinase [Source:HGNC Symbol;Acc:HGNC:1148] MAGEA MAGE family member A10 [Source:HGNC Symbol;Acc:HGNC:6797] MAGEA10-MAGEA MAGEA10-MAGEA5 Readthrough ANKRD ankyrin repeat domain 17 [Source:HGNC Symbol;Acc:HGNC:23575] TMEM transmembrane protein 40 [Source:HGNC Symbol;Acc:HGNC:25620] AFTPH aftiphilin [Source:HGNC Symbol;Acc:HGNC:25951] ATP2B1 490 ATPase plasma membrane Ca2+ transporting 1 [Source:HGNC Symbol;Acc:HGNC:814] DCAF DDB1 and CUL4 associated factor 13 [Source:HGNC Symbol;Acc:HGNC:24535] SLC25A solute carrier family 25 member 32 [Source:HGNC Symbol;Acc:HGNC:29683] GFI1B 8328 growth factor independent 1B transcriptional repressor [Source:HGNC Symbol;Acc:HGNC:4238] S100A S100 calcium binding protein A8 [Source:HGNC Symbol;Acc:HGNC:10498] TMEM87A transmembrane protein 87A [Source:HGNC Symbol;Acc:HGNC:24522] ANKRD ankyrin repeat domain 10 [Source:HGNC Symbol;Acc:HGNC:20265] PTN 5764 pleiotrophin [Source:HGNC Symbol;Acc:HGNC:9630] TRIP thyroid hormone receptor interactor 12 [Source:HGNC Symbol;Acc:HGNC:12306] KRT keratin 15 [Source:HGNC Symbol;Acc:HGNC:6421] ITGB integrin subunit beta 2 [Source:HGNC Symbol;Acc:HGNC:6155] WDR45B WD repeat domain 45B [Source:HGNC Symbol;Acc:HGNC:25072] IFT intraflagellar transport 20 [Source:HGNC Symbol;Acc:HGNC:30989] FGR 2268 FGR proto-oncogene, Src family tyrosine kinase [Source:HGNC Symbol;Acc:HGNC:3697] NR2C nuclear receptor subfamily 2 group C member 2 [Source:HGNC Symbol;Acc:HGNC:7972] TRA2A transformer 2 alpha homolog [Source:HGNC Symbol;Acc:HGNC:16645] MT-ND mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 6 [Source:HGNC Symbol;Acc:HGNC:7462] NUP nucleoporin 93 [Source:HGNC Symbol;Acc:HGNC:28958] MAGEB MAGE family member B1 [Source:HGNC Symbol;Acc:HGNC:6808] MAGEB MAGE family member B4 [Source:HGNC Symbol;Acc:HGNC:6811] ATP5S ATP synthase, H+ transporting, mitochondrial Fo complex subunit s (factor B) [Source:HGNC Symbol;Acc:HGNC:18799] C2orf chromosome 2 open reading frame 88 [Source:HGNC Symbol;Acc:HGNC:28191] PAEP 5047 progestagen associated endometrial protein [Source:HGNC Symbol;Acc:HGNC:8573] SAMD sterile alpha motif domain containing 14 [Source:HGNC Symbol;Acc:HGNC:27312] TMEM106C transmembrane protein 106C [Source:HGNC Symbol;Acc:HGNC:28775] SPPL2B signal peptide peptidase like 2B [Source:HGNC Symbol;Acc:HGNC:30627] CD CD247 molecule [Source:HGNC Symbol;Acc:HGNC:1677] NUSAP nucleolar and spindle associated protein 1 [Source:HGNC Symbol;Acc:HGNC:18538] KIF5C 3800 kinesin family member 5C [Source:HGNC Symbol;Acc:HGNC:6325] TRIM tripartite motif containing 58 [Source:HGNC Symbol;Acc:HGNC:24150] MKKS 8195 McKusick-Kaufman syndrome [Source:HGNC Symbol;Acc:HGNC:7108] ERN endoplasmic reticulum to nucleus signaling 2 [Source:HGNC Symbol;Acc:HGNC:16942] PLK polo like kinase 1 [Source:HGNC Symbol;Acc:HGNC:9077] POM POM121 transmembrane nucleoporin [Source:HGNC Symbol;Acc:HGNC:19702] TMEM transmembrane protein 11 [Source:HGNC Symbol;Acc:HGNC:16823] SNRNP small nuclear ribonucleoprotein U11/U12 subunit 25 [Source:HGNC Symbol;Acc:HGNC:14161] PRAM PML-RARA regulated adaptor molecule 1 [Source:HGNC Symbol;Acc:HGNC:30091] GMPR guanosine monophosphate reductase 2 [Source:HGNC Symbol;Acc:HGNC:4377] ILF interleukin enhancer binding factor 3 [Source:HGNC Symbol;Acc:HGNC:6038] LUZP leucine zipper protein 4 [Source:HGNC Symbol;Acc:HGNC:24971] NUP nucleoporin 35 [Source:HGNC Symbol;Acc:HGNC:29797] SMPD sphingomyelin phosphodiesterase 1 [Source:HGNC Symbol;Acc:HGNC:11120] MBD methyl-cpg binding domain protein 6 [Source:HGNC Symbol;Acc:HGNC:20445] APOBEC3A apolipoprotein B mrna editing enzyme catalytic subunit 3A [Source:HGNC Symbol;Acc:HGNC:17343] UNC13B unc-13 homolog B (C. elegans) [Source:HGNC Symbol;Acc:HGNC:12566] TIMM8B translocase of inner mitochondrial membrane 8 homolog B (yeast) [Source:HGNC Symbol;Acc:HGNC:11818] C6orf chromosome 6 open reading frame 62 [Source:HGNC Symbol;Acc:HGNC:20998] YWHAG 7532 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma [Source:HGNC Symbol;Acc:HGNC:12852] FAM133B family with sequence similarity 133 member B [Source:HGNC Symbol;Acc:HGNC:28629] CKAP cytoskeleton associated protein 2 [Source:HGNC Symbol;Acc:HGNC:1990] MAGEC MAGE family member C2 [Source:HGNC Symbol;Acc:HGNC:13574] ARL6IP ADP ribosylation factor like GTPase 6 interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:697] ALDH9A1 223 aldehyde dehydrogenase 9 family member A1 [Source:HGNC Symbol;Acc:HGNC:412] UBE2V ubiquitin conjugating enzyme E2 V2 [Source:HGNC Symbol;Acc:HGNC:12495] LILRA leukocyte immunoglobulin like receptor A2 [Source:HGNC Symbol;Acc:HGNC:6603] GP glycoprotein IX platelet [Source:HGNC Symbol;Acc:HGNC:4444] PDZK1IP PDZK1 interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:16887] 4.9 2

10 145 MAGEB MAGE family member B2 [Source:HGNC Symbol;Acc:HGNC:6809] PPP2R2A 5520 protein phosphatase 2 regulatory subunit Balpha [Source:HGNC Symbol;Acc:HGNC:9304] C12orf chromosome 12 open reading frame 75 [Source:HGNC Symbol;Acc:HGNC:35164] PRKAR2B 5577 protein kinase camp-dependent type II regulatory subunit beta [Source:HGNC Symbol;Acc:HGNC:9392] CFP 5199 complement factor properdin [Source:HGNC Symbol;Acc:HGNC:8864] DNAJB DnaJ heat shock protein family (Hsp40) member B7 [Source:HGNC Symbol;Acc:HGNC:24986] XPNPEP X-prolyl aminopeptidase 3, mitochondrial [Source:HGNC Symbol;Acc:HGNC:28052] SELENBP selenium binding protein 1 [Source:HGNC Symbol;Acc:HGNC:10719] SMU DNA replication regulator and spliceosomal factor [Source:HGNC Symbol;Acc:HGNC:18247] SUMO small ubiquitin-like modifier 2 [Source:HGNC Symbol;Acc:HGNC:11125] SEC61B Sec61 translocon beta subunit [Source:HGNC Symbol;Acc:HGNC:16993] PEF penta-ef-hand domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30009] PRKCB 5579 protein kinase C beta [Source:HGNC Symbol;Acc:HGNC:9395] TOP2B 7155 topoisomerase (DNA) II beta [Source:HGNC Symbol;Acc:HGNC:11990] HK hexokinase 3 [Source:HGNC Symbol;Acc:HGNC:4925] SFSWAP 6433 splicing factor SWAP homolog [Source:HGNC Symbol;Acc:HGNC:10790] PRRC2C proline rich coiled-coil 2C [Source:HGNC Symbol;Acc:HGNC:24903] COA cytochrome c oxidase assembly factor 1 homolog [Source:HGNC Symbol;Acc:HGNC:21868] LILRB leukocyte immunoglobulin like receptor B2 [Source:HGNC Symbol;Acc:HGNC:6606] CCT chaperonin containing TCP1 subunit 2 [Source:HGNC Symbol;Acc:HGNC:1615] MAGED MAGE family member D2 [Source:HGNC Symbol;Acc:HGNC:16353] CDC cell division cycle 20 [Source:HGNC Symbol;Acc:HGNC:1723] SNRPA small nuclear ribonucleoprotein polypeptide A' [Source:HGNC Symbol;Acc:HGNC:11152] MAP3K7CL MAP3K7 C-terminal like [Source:HGNC Symbol;Acc:HGNC:16457] PADI peptidyl arginine deiminase 4 [Source:HGNC Symbol;Acc:HGNC:18368] SPX spexin hormone [Source:HGNC Symbol;Acc:HGNC:28139] GZMB 3002 granzyme B [Source:HGNC Symbol;Acc:HGNC:4709] RASGRP RAS guanyl releasing protein 2 [Source:HGNC Symbol;Acc:HGNC:9879] FAM133A family with sequence similarity 133 member A [Source:HGNC Symbol;Acc:HGNC:26748] MPL 4352 MPL proto-oncogene, thrombopoietin receptor [Source:HGNC Symbol;Acc:HGNC:7217] APOBEC3B 9582 apolipoprotein B mrna editing enzyme catalytic subunit 3B [Source:HGNC Symbol;Acc:HGNC:17352] PTGS prostaglandin-endoperoxide synthase 1 [Source:HGNC Symbol;Acc:HGNC:9604] TIMM17A translocase of inner mitochondrial membrane 17 homolog A (yeast) [Source:HGNC Symbol;Acc:HGNC:17315] NFE nuclear factor, erythroid 2 [Source:HGNC Symbol;Acc:HGNC:7780] MSN 4478 moesin [Source:HGNC Symbol;Acc:HGNC:7373] CCNL cyclin L1 [Source:HGNC Symbol;Acc:HGNC:20569] NUP nucleoporin 37 [Source:HGNC Symbol;Acc:HGNC:29929] TMEM transmembrane protein 91 [Source:HGNC Symbol;Acc:HGNC:32393] ZNF zinc finger protein 185 (LIM domain) [Source:HGNC Symbol;Acc:HGNC:12976] PMM phosphomannomutase 1 [Source:HGNC Symbol;Acc:HGNC:9114] USP ubiquitin specific peptidase 1 [Source:HGNC Symbol;Acc:HGNC:12607] DLGAP DLG associated protein 5 [Source:HGNC Symbol;Acc:HGNC:16864] TROAP trophinin associated protein [Source:HGNC Symbol;Acc:HGNC:12327] LUC7L LUC7 like 3 pre-mrna splicing factor [Source:HGNC Symbol;Acc:HGNC:24309] SRPRB SRP receptor beta subunit [Source:HGNC Symbol;Acc:HGNC:24085] NFIL nuclear factor, interleukin 3 regulated [Source:HGNC Symbol;Acc:HGNC:7787] ADD3 120 adducin 3 [Source:HGNC Symbol;Acc:HGNC:245] TAP transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) [Source:HGNC Symbol;Acc:HGNC:43] DLD 1738 dihydrolipoamide dehydrogenase [Source:HGNC Symbol;Acc:HGNC:2898] SLC25A solute carrier family 25 member 38 [Source:HGNC Symbol;Acc:HGNC:26054] PLOD procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 [Source:HGNC Symbol;Acc:HGNC:9081] PABPN poly(a) binding protein nuclear 1 [Source:HGNC Symbol;Acc:HGNC:8565] GAL3ST galactose-3-o-sulfotransferase 4 [Source:HGNC Symbol;Acc:HGNC:24145] DZIP DAZ interacting zinc finger protein 3 [Source:HGNC Symbol;Acc:HGNC:30938] KIAA KIAA1524 [Source:HGNC Symbol;Acc:HGNC:29302] C10orf chromosome 10 open reading frame 54 [Source:HGNC Symbol;Acc:HGNC:30085] MFAP3L 9848 microfibrillar associated protein 3 like [Source:HGNC Symbol;Acc:HGNC:29083] CTTN 2017 cortactin [Source:HGNC Symbol;Acc:HGNC:3338] VPS VPS28, ESCRT-I subunit [Source:HGNC Symbol;Acc:HGNC:18178] DCTN dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:2713] CCDC coiled-coil domain containing 152 [Source:HGNC Symbol;Acc:HGNC:34438] SEPP selenoprotein P, plasma, 1 [Source:HGNC Symbol;Acc:HGNC:10751] PSMC proteasome 26S subunit, ATPase 2 [Source:HGNC Symbol;Acc:HGNC:9548] TMED transmembrane p24 trafficking protein 2 [Source:HGNC Symbol;Acc:HGNC:16996] EIF2S eukaryotic translation initiation factor 2 subunit beta [Source:HGNC Symbol;Acc:HGNC:3266] S100A S100 calcium binding protein A9 [Source:HGNC Symbol;Acc:HGNC:10499] SYTL synaptotagmin like 5 [Source:HGNC Symbol;Acc:HGNC:15589] RIN Ras and Rab interactor 3 [Source:HGNC Symbol;Acc:HGNC:18751] HNRNPH heterogeneous nuclear ribonucleoprotein H3 [Source:HGNC Symbol;Acc:HGNC:5043] CMTM CKLF like MARVEL transmembrane domain containing 5 [Source:HGNC Symbol;Acc:HGNC:19176] FAM136A family with sequence similarity 136 member A [Source:HGNC Symbol;Acc:HGNC:25911] SYCP synaptonemal complex protein 3 [Source:HGNC Symbol;Acc:HGNC:18130] C11orf chromosome 11 open reading frame 21 [Source:HGNC Symbol;Acc:HGNC:13231] GNG G protein subunit gamma 10 [Source:HGNC Symbol;Acc:HGNC:4402] 4.6 2

11 219 DNAJC25-GNG DNAJC25-GNG10 readthrough [Source:HGNC Symbol;Acc:HGNC:37501] DAPP dual adaptor of phosphotyrosine and 3-phosphoinositides 1 [Source:HGNC Symbol;Acc:HGNC:16500] MRPL mitochondrial ribosomal protein L22 [Source:HGNC Symbol;Acc:HGNC:14480] HJURP Holliday junction recognition protein [Source:HGNC Symbol;Acc:HGNC:25444] ALDH3A2 224 aldehyde dehydrogenase 3 family member A2 [Source:HGNC Symbol;Acc:HGNC:403] SPATA spermatogenesis associated 20 [Source:HGNC Symbol;Acc:HGNC:26125] C4orf chromosome 4 open reading frame 19 [Source:HGNC Symbol;Acc:HGNC:25618] RELL RELT like 1 [Source:HGNC Symbol;Acc:HGNC:27379] TSPAN tetraspanin 33 [Source:HGNC Symbol;Acc:HGNC:28743] CLEC12A C-type lectin domain family 12 member A [Source:HGNC Symbol;Acc:HGNC:31713] C19orf chromosome 19 open reading frame 33 [Source:HGNC Symbol;Acc:HGNC:16668] UGP UDP-glucose pyrophosphorylase 2 [Source:HGNC Symbol;Acc:HGNC:12527] CCDC coiled-coil domain containing 169 [Source:HGNC Symbol;Acc:HGNC:34361] CCDC169-SOHLH CCDC169-SOHLH2 readthrough [Source:HGNC Symbol;Acc:HGNC:38866] SOHLH spermatogenesis and oogenesis specific basic helix-loop-helix 2 [Source:HGNC Symbol;Acc:HGNC:26026] NOV 4856 nephroblastoma overexpressed [Source:HGNC Symbol;Acc:HGNC:7885] ATF3 467 activating transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:785] PSMA proteasome subunit alpha 5 [Source:HGNC Symbol;Acc:HGNC:9534] AURKA 6790 aurora kinase A [Source:HGNC Symbol;Acc:HGNC:11393] PRF perforin 1 [Source:HGNC Symbol;Acc:HGNC:9360] DNAJA DnaJ heat shock protein family (Hsp40) member A2 [Source:HGNC Symbol;Acc:HGNC:14884] CSF3R 1441 colony stimulating factor 3 receptor [Source:HGNC Symbol;Acc:HGNC:2439] CHORDC cysteine and histidine rich domain containing 1 [Source:HGNC Symbol;Acc:HGNC:14525] NCF neutrophil cytosolic factor 4 [Source:HGNC Symbol;Acc:HGNC:7662] RACGAP Rac GTPase activating protein 1 [Source:HGNC Symbol;Acc:HGNC:9804] TCEAL transcription elongation factor A like 8 [Source:HGNC Symbol;Acc:HGNC:28683] S100A S100 calcium binding protein A16 [Source:HGNC Symbol;Acc:HGNC:20441] GFOD glucose-fructose oxidoreductase domain containing 1 [Source:HGNC Symbol;Acc:HGNC:21096] RUFY RUN and FYVE domain containing 1 [Source:HGNC Symbol;Acc:HGNC:19760] EIF eukaryotic translation initiation factor 5 [Source:HGNC Symbol;Acc:HGNC:3299] PGM phosphoglucomutase 2 [Source:HGNC Symbol;Acc:HGNC:8906] ASNS 440 asparagine synthetase (glutamine-hydrolyzing) [Source:HGNC Symbol;Acc:HGNC:753] LILRA leukocyte immunoglobulin like receptor A3 [Source:HGNC Symbol;Acc:HGNC:6604] MRRF mitochondrial ribosome recycling factor [Source:HGNC Symbol;Acc:HGNC:7234] YY YY1 transcription factor [Source:HGNC Symbol;Acc:HGNC:12856] TOP topoisomerase (DNA) I [Source:HGNC Symbol;Acc:HGNC:11986] FAM192A family with sequence similarity 192 member A [Source:HGNC Symbol;Acc:HGNC:29856] DRAP DR1 associated protein 1 [Source:HGNC Symbol;Acc:HGNC:3019] OGT 8473 O-linked N-acetylglucosamine (GlcNAc) transferase [Source:HGNC Symbol;Acc:HGNC:8127] TCAF TRPM8 channel associated factor 1 [Source:HGNC Symbol;Acc:HGNC:22201] ITM2A 9452 integral membrane protein 2A [Source:HGNC Symbol;Acc:HGNC:6173] SLC25A solute carrier family 25 member 36 [Source:HGNC Symbol;Acc:HGNC:25554] RAP1GDS Rap1 GTPase-GDP dissociation stimulator 1 [Source:HGNC Symbol;Acc:HGNC:9859] PPM1A 5494 protein phosphatase, Mg2+/Mn2+ dependent 1A [Source:HGNC Symbol;Acc:HGNC:9275] DPEP dipeptidase 2 [Source:HGNC Symbol;Acc:HGNC:23028] HBA hemoglobin subunit alpha 2 [Source:HGNC Symbol;Acc:HGNC:4824] UBE2E ubiquitin conjugating enzyme E2 E3 [Source:HGNC Symbol;Acc:HGNC:12479] WIPI WD repeat domain, phosphoinositide interacting 1 [Source:HGNC Symbol;Acc:HGNC:25471] CHD chromodomain helicase DNA binding protein 4 [Source:HGNC Symbol;Acc:HGNC:1919] GP1BA 2811 glycoprotein Ib platelet alpha subunit [Source:HGNC Symbol;Acc:HGNC:4439] BEND BEN domain containing 2 [Source:HGNC Symbol;Acc:HGNC:28509] COMMD COMM domain containing 7 [Source:HGNC Symbol;Acc:HGNC:16223] FLII 2314 FLII, actin remodeling protein [Source:HGNC Symbol;Acc:HGNC:3750] CHMP charged multivesicular body protein 5 [Source:HGNC Symbol;Acc:HGNC:26942] ADRM adhesion regulating molecule 1 [Source:HGNC Symbol;Acc:HGNC:15759] GZMH 2999 granzyme H [Source:HGNC Symbol;Acc:HGNC:4710] RABIF 5877 RAB interacting factor [Source:HGNC Symbol;Acc:HGNC:9797] PBK PDZ binding kinase [Source:HGNC Symbol;Acc:HGNC:18282] PROK prokineticin 2 [Source:HGNC Symbol;Acc:HGNC:18455] ANKRD ankyrin repeat domain 55 [Source:HGNC Symbol;Acc:HGNC:25681] PAGE2B PAGE family member 2B [Source:HGNC Symbol;Acc:HGNC:31805] GNLY granulysin [Source:HGNC Symbol;Acc:HGNC:4414] SSB 6741 Sjogren syndrome antigen B [Source:HGNC Symbol;Acc:HGNC:11316] RBM RNA binding motif protein 22 [Source:HGNC Symbol;Acc:HGNC:25503] MCL myeloid cell leukemia 1 [Source:HGNC Symbol;Acc:HGNC:6943] OSTC oligosaccharyltransferase complex subunit (non-catalytic) [Source:HGNC Symbol;Acc:HGNC:24448] PILRB paired immunoglobin-like type 2 receptor beta [Source:HGNC Symbol;Acc:HGNC:18297] SPDYE speedy/ringo cell cycle regulator family member E3 [Source:HGNC Symbol;Acc:HGNC:35462] ZNF zinc finger protein 467 [Source:HGNC Symbol;Acc:HGNC:23154] SRPX 8406 sushi repeat containing protein, X-linked [Source:HGNC Symbol;Acc:HGNC:11309] CCDC coiled-coil domain containing 73 [Source:HGNC Symbol;Acc:HGNC:23261] TTK 7272 TTK protein kinase [Source:HGNC Symbol;Acc:HGNC:12401] AKIRIN akirin 1 [Source:HGNC Symbol;Acc:HGNC:25744] IST IST1, ESCRT-III associated factor [Source:HGNC Symbol;Acc:HGNC:28977] SRP signal recognition particle 72kDa [Source:HGNC Symbol;Acc:HGNC:11303] CLUL clusterin like 1 [Source:HGNC Symbol;Acc:HGNC:2096] 4.3 2

12 295 ENOSF enolase superfamily member 1 [Source:HGNC Symbol;Acc:HGNC:30365] TYMS 7298 thymidylate synthetase [Source:HGNC Symbol;Acc:HGNC:12441] TYMSOS TYMS opposite strand [Source:HGNC Symbol;Acc:HGNC:29553] APOD 347 apolipoprotein D [Source:HGNC Symbol;Acc:HGNC:612] CENPK centromere protein K [Source:HGNC Symbol;Acc:HGNC:29479] SURF surfeit 2 [Source:HGNC Symbol;Acc:HGNC:11475] C5orf chromosome 5 open reading frame 15 [Source:HGNC Symbol;Acc:HGNC:20656] LARP La ribonucleoprotein domain family member 4 [Source:HGNC Symbol;Acc:HGNC:24320] FRMD FERM domain containing 3 [Source:HGNC Symbol;Acc:HGNC:24125] CTNNAL catenin alpha like 1 [Source:HGNC Symbol;Acc:HGNC:2512] LGMN 5641 legumain [Source:HGNC Symbol;Acc:HGNC:9472] MMP matrix metallopeptidase 9 [Source:HGNC Symbol;Acc:HGNC:7176] C8orf chromosome 8 open reading frame 76 [Source:HGNC Symbol;Acc:HGNC:25924] ZHX zinc fingers and homeoboxes 1 [Source:HGNC Symbol;Acc:HGNC:12871] ZHX1-C8orf ZHX1-C8orf76 readthrough [Source:HGNC Symbol;Acc:HGNC:42975] HN hematological and neurological expressed 1 [Source:HGNC Symbol;Acc:HGNC:14569] TNFRSF1B 7133 tumor necrosis factor receptor superfamily member 1B [Source:HGNC Symbol;Acc:HGNC:11917] MSMO methylsterol monooxygenase 1 [Source:HGNC Symbol;Acc:HGNC:10545] ARL4C ADP ribosylation factor like GTPase 4C [Source:HGNC Symbol;Acc:HGNC:698] LTB4R 1241 leukotriene B4 receptor [Source:HGNC Symbol;Acc:HGNC:6713] TTC tetratricopeptide repeat domain 1 [Source:HGNC Symbol;Acc:HGNC:12391] HMGB high mobility group box 2 [Source:HGNC Symbol;Acc:HGNC:5000] TMEM170B transmembrane protein 170B [Source:HGNC Symbol;Acc:HGNC:34244] PUM pumilio RNA binding family member 3 [Source:HGNC Symbol;Acc:HGNC:29676] HOMER homer scaffolding protein 2 [Source:HGNC Symbol;Acc:HGNC:17513] XRRA X-ray radiation resistance associated 1 [Source:HGNC Symbol;Acc:HGNC:18868] SNN 8303 stannin [Source:HGNC Symbol;Acc:HGNC:11149] MTERF mitochondrial transcription termination factor 2 [Source:HGNC Symbol;Acc:HGNC:30779] FGD FYVE, RhoGEF and PH domain containing 3 [Source:HGNC Symbol;Acc:HGNC:16027] CCDC coiled-coil domain containing 174 [Source:HGNC Symbol;Acc:HGNC:28033] GLT1D glycosyltransferase 1 domain containing 1 [Source:HGNC Symbol;Acc:HGNC:26483] NKIRAS NFKB inhibitor interacting Ras like 1 [Source:HGNC Symbol;Acc:HGNC:17899] FERMT fermitin family member 3 [Source:HGNC Symbol;Acc:HGNC:23151] EGLN egl-9 family hypoxia inducible factor 2 [Source:HGNC Symbol;Acc:HGNC:14660] USP ubiquitin specific peptidase 48 [Source:HGNC Symbol;Acc:HGNC:18533] CNDP carnosine dipeptidase 1 [Source:HGNC Symbol;Acc:HGNC:20675] FAM65B 9750 family with sequence similarity 65 member B [Source:HGNC Symbol;Acc:HGNC:13872] LARP La ribonucleoprotein domain family member 1 [Source:HGNC Symbol;Acc:HGNC:29531] AQP aquaporin 10 [Source:HGNC Symbol;Acc:HGNC:16029] FAM168B family with sequence similarity 168 member B [Source:HGNC Symbol;Acc:HGNC:27016] TMEM200B transmembrane protein 200B [Source:HGNC Symbol;Acc:HGNC:33785] P2RY purinergic receptor P2Y12 [Source:HGNC Symbol;Acc:HGNC:18124] CLU 1191 clusterin [Source:HGNC Symbol;Acc:HGNC:2095] DDX3Y 8653 DEAD-box helicase 3, Y-linked [Source:HGNC Symbol;Acc:HGNC:2699] STXBP syntaxin binding protein 2 [Source:HGNC Symbol;Acc:HGNC:11445] AK4 205 adenylate kinase 4 [Source:HGNC Symbol;Acc:HGNC:363] BST2 684 bone marrow stromal cell antigen 2 [Source:HGNC Symbol;Acc:HGNC:1119] SRRM serine and arginine repetitive matrix 1 [Source:HGNC Symbol;Acc:HGNC:16638] KXD KxDL motif containing 1 [Source:HGNC Symbol;Acc:HGNC:28420] PABPC poly(a) binding protein cytoplasmic 3 [Source:HGNC Symbol;Acc:HGNC:8556] PSMD proteasome 26S subunit, non-atpase 4 [Source:HGNC Symbol;Acc:HGNC:9561] TBXA2R 6915 thromboxane A2 receptor [Source:HGNC Symbol;Acc:HGNC:11608] ZWINT ZW10 interacting kinetochore protein [Source:HGNC Symbol;Acc:HGNC:13195] VAMP vesicle associated membrane protein 1 [Source:HGNC Symbol;Acc:HGNC:12642] HMHA Mitochondrial Calcium Uniporter RAD23A 5886 RAD23 homolog A, nucleotide excision repair protein [Source:HGNC Symbol;Acc:HGNC:9812] PIN peptidylprolyl cis/trans isomerase, NIMA-interacting 4 [Source:HGNC Symbol;Acc:HGNC:8992] DGAT diacylglycerol O-acyltransferase 1 [Source:HGNC Symbol;Acc:HGNC:2843] OVOS Ovostatin IQCJ IQ motif containing J [Source:HGNC Symbol;Acc:HGNC:32406] IQCJ-SCHIP IQCJ-SCHIP1 readthrough [Source:HGNC Symbol;Acc:HGNC:38842] SCHIP schwannomin interacting protein 1 [Source:HGNC Symbol;Acc:HGNC:15678] PTPRJ 5795 protein tyrosine phosphatase, receptor type J [Source:HGNC Symbol;Acc:HGNC:9673] DGAT diacylglycerol O-acyltransferase 2 [Source:HGNC Symbol;Acc:HGNC:16940] KCNN potassium calcium-activated channel subfamily N member 2 [Source:HGNC Symbol;Acc:HGNC:6291] CORO1A coronin 1A [Source:HGNC Symbol;Acc:HGNC:2252] UBE2N 7334 ubiquitin conjugating enzyme E2 N [Source:HGNC Symbol;Acc:HGNC:12492] TMEM transmembrane protein 97 [Source:HGNC Symbol;Acc:HGNC:28106] GAS2L growth arrest specific 2 like 1 [Source:HGNC Symbol;Acc:HGNC:16955] TNC 3371 tenascin C [Source:HGNC Symbol;Acc:HGNC:5318] SLC25A5 292 solute carrier family 25 member 5 [Source:HGNC Symbol;Acc:HGNC:10991] DNM1L dynamin 1-like [Source:HGNC Symbol;Acc:HGNC:2973] EIF1AY 9086 eukaryotic translation initiation factor 1A, Y-linked [Source:HGNC Symbol;Acc:HGNC:3252] HMOX heme oxygenase 1 [Source:HGNC Symbol;Acc:HGNC:5013] FKBP FK506 binding protein 3 [Source:HGNC Symbol;Acc:HGNC:3719] CD CD36 molecule [Source:HGNC Symbol;Acc:HGNC:1663] MAEA macrophage erythroblast attacher [Source:HGNC Symbol;Acc:HGNC:13731] DOK docking protein 3 [Source:HGNC Symbol;Acc:HGNC:24583] PXN 5829 paxillin [Source:HGNC Symbol;Acc:HGNC:9718] 4.2 2

13 374 NUDT nudix hydrolase 1 [Source:HGNC Symbol;Acc:HGNC:8048] PCGF polycomb group ring finger 3 [Source:HGNC Symbol;Acc:HGNC:10066] SDHB 6390 succinate dehydrogenase complex iron sulfur subunit B [Source:HGNC Symbol;Acc:HGNC:10681] SEMA4D semaphorin 4D [Source:HGNC Symbol;Acc:HGNC:10732] RNF ring finger protein 5 [Source:HGNC Symbol;Acc:HGNC:10068] HIST2H2BE 8349 histone cluster 2, H2be [Source:HGNC Symbol;Acc:HGNC:4760] IQGAP IQ motif containing GTPase activating protein 3 [Source:HGNC Symbol;Acc:HGNC:20669] PRPF pre-mrna processing factor 31 [Source:HGNC Symbol;Acc:HGNC:15446] FNTA 2339 farnesyltransferase, CAAX box, alpha [Source:HGNC Symbol;Acc:HGNC:3782] CCR C-C motif chemokine receptor 7 [Source:HGNC Symbol;Acc:HGNC:1608] RSL24D ribosomal L24 domain containing 1 [Source:HGNC Symbol;Acc:HGNC:18479] UBE2L ubiquitin conjugating enzyme E2 L6 [Source:HGNC Symbol;Acc:HGNC:12490] ITGB integrin subunit beta 3 [Source:HGNC Symbol;Acc:HGNC:6156] PAGE PAGE family member 2 [Source:HGNC Symbol;Acc:HGNC:31804] PTCRA pre T-cell antigen receptor alpha [Source:HGNC Symbol;Acc:HGNC:21290] LCN lipocalin 2 [Source:HGNC Symbol;Acc:HGNC:6526] NBEAL neurobeachin like 2 [Source:HGNC Symbol;Acc:HGNC:31928] SUV420H Lysine Methyltransferase 5B ANKRD ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:HGNC:29024] SCLY selenocysteine lyase [Source:HGNC Symbol;Acc:HGNC:18161] ALDH18A aldehyde dehydrogenase 18 family member A1 [Source:HGNC Symbol;Acc:HGNC:9722] SERPINE serpin family E member 1 [Source:HGNC Symbol;Acc:HGNC:8583] CEP centrosomal protein 57 [Source:HGNC Symbol;Acc:HGNC:30794] ZNF zinc finger protein 263 [Source:HGNC Symbol;Acc:HGNC:13056] TCTN tectonic family member 3 [Source:HGNC Symbol;Acc:HGNC:24519] SLC25A solute carrier family 25 member 10 [Source:HGNC Symbol;Acc:HGNC:10980] CRIM cysteine rich transmembrane BMP regulator 1 (chordin-like) [Source:HGNC Symbol;Acc:HGNC:2359] SLC11A solute carrier family 11 member 1 [Source:HGNC Symbol;Acc:HGNC:10907] FAM110A family with sequence similarity 110 member A [Source:HGNC Symbol;Acc:HGNC:16188] CCDC coiled-coil domain containing 58 [Source:HGNC Symbol;Acc:HGNC:31136] TMCO transmembrane and coiled-coil domains 1 [Source:HGNC Symbol;Acc:HGNC:18188] LGALSL galectin like [Source:HGNC Symbol;Acc:HGNC:25012] XRCC X-ray repair cross complementing 4 [Source:HGNC Symbol;Acc:HGNC:12831] TPM tropomyosin 1 (alpha) [Source:HGNC Symbol;Acc:HGNC:12010] CXCR C-X-C motif chemokine receptor 2 [Source:HGNC Symbol;Acc:HGNC:6027] MYL myosin light chain 9 [Source:HGNC Symbol;Acc:HGNC:15754] KRTCAP keratinocyte associated protein 3 [Source:HGNC Symbol;Acc:HGNC:28943] PHF PHD finger protein 6 [Source:HGNC Symbol;Acc:HGNC:18145] FPR formyl peptide receptor 1 [Source:HGNC Symbol;Acc:HGNC:3826] ZBTB zinc finger and BTB domain containing 44 [Source:HGNC Symbol;Acc:HGNC:25001] CD CD37 molecule [Source:HGNC Symbol;Acc:HGNC:1666] FAM162A family with sequence similarity 162 member A [Source:HGNC Symbol;Acc:HGNC:17865] MARCH membrane associated ring-ch-type finger 2 [Source:HGNC Symbol;Acc:HGNC:28038] STX syntaxin 11 [Source:HGNC Symbol;Acc:HGNC:11429] NOP NOP56 ribonucleoprotein [Source:HGNC Symbol;Acc:HGNC:15911] CLEC2B 9976 C-type lectin domain family 2 member B [Source:HGNC Symbol;Acc:HGNC:2053] RBCK RANBP2-type and C3HC4-type zinc finger containing 1 [Source:HGNC Symbol;Acc:HGNC:15864] CYB5A 1528 cytochrome b5 type A [Source:HGNC Symbol;Acc:HGNC:2570] METAP methionyl aminopeptidase 2 [Source:HGNC Symbol;Acc:HGNC:16672] ADCY7 113 adenylate cyclase 7 [Source:HGNC Symbol;Acc:HGNC:238] ESAM endothelial cell adhesion molecule [Source:HGNC Symbol;Acc:HGNC:17474] EPCAM 4072 epithelial cell adhesion molecule [Source:HGNC Symbol;Acc:HGNC:11529] BIRC5 332 baculoviral IAP repeat containing 5 [Source:HGNC Symbol;Acc:HGNC:593] MEGF multiple EGF like domains 9 [Source:HGNC Symbol;Acc:HGNC:3234] DECR ,4-dienoyl-CoA reductase 1, mitochondrial [Source:HGNC Symbol;Acc:HGNC:2753] RNF ring finger protein 34 [Source:HGNC Symbol;Acc:HGNC:17297] PRPF40A pre-mrna processing factor 40 homolog A [Source:HGNC Symbol;Acc:HGNC:16463] CCL C-C motif chemokine ligand 5 [Source:HGNC Symbol;Acc:HGNC:10632] NFIC 4782 nuclear factor I C [Source:HGNC Symbol;Acc:HGNC:7786] SMIM small integral membrane protein 5 [Source:HGNC Symbol;Acc:HGNC:40030] PLAA 9373 phospholipase A2 activating protein [Source:HGNC Symbol;Acc:HGNC:9043] CR complement component 3b/4b receptor 1 (Knops blood group) [Source:HGNC Symbol;Acc:HGNC:2334] VNN vanin 2 [Source:HGNC Symbol;Acc:HGNC:12706] CENPH centromere protein H [Source:HGNC Symbol;Acc:HGNC:17268] VAPB 9217 VAMP (vesicle-associated membrane protein)-associated protein B and C [Source:HGNC Symbol;Acc:HGNC:12649] HBG hemoglobin subunit gamma 2 [Source:HGNC Symbol;Acc:HGNC:4832] DOK docking protein 2 [Source:HGNC Symbol;Acc:HGNC:2991] PCSK proprotein convertase subtilisin/kexin type 6 [Source:HGNC Symbol;Acc:HGNC:8569] HGD 3081 homogentisate 1,2-dioxygenase [Source:HGNC Symbol;Acc:HGNC:4892] GRINA 2907 glutamate ionotropic receptor NMDA type subunit associated protein 1 [Source:HGNC Symbol;Acc:HGNC:4589] PPP1R protein phosphatase 1 regulatory subunit 10 [Source:HGNC Symbol;Acc:HGNC:9284] ARHGAP6 395 Rho GTPase activating protein 6 [Source:HGNC Symbol;Acc:HGNC:676] ZDHHC zinc finger DHHC-type containing 9 [Source:HGNC Symbol;Acc:HGNC:18475] AKAP A-kinase anchoring protein 1 [Source:HGNC Symbol;Acc:HGNC:367] UNC119B unc-119 lipid binding chaperone B [Source:HGNC Symbol;Acc:HGNC:16488] VPS37C VPS37C, ESCRT-I subunit [Source:HGNC Symbol;Acc:HGNC:26097] 4.0 2

14 450 LYL lymphoblastic leukemia associated hematopoiesis regulator 1 [Source:HGNC Symbol;Acc:HGNC:6734] ERAP endoplasmic reticulum aminopeptidase 2 [Source:HGNC Symbol;Acc:HGNC:29499] IKZF IKAROS family zinc finger 1 [Source:HGNC Symbol;Acc:HGNC:13176] SELL 6402 selectin L [Source:HGNC Symbol;Acc:HGNC:10720] CBR carbonyl reductase 4 [Source:HGNC Symbol;Acc:HGNC:25891] CMTM CKLF like MARVEL transmembrane domain containing 2 [Source:HGNC Symbol;Acc:HGNC:19173] NAA N(alpha)-acetyltransferase 50, NatE catalytic subunit [Source:HGNC Symbol;Acc:HGNC:29533] LAMTOR late endosomal/lysosomal adaptor, MAPK and MTOR activator 5 [Source:HGNC Symbol;Acc:HGNC:17955] CCDC coiled-coil domain containing 86 [Source:HGNC Symbol;Acc:HGNC:28359] HDAC histone deacetylase 3 [Source:HGNC Symbol;Acc:HGNC:4854] NPFFR neuropeptide FF receptor 2 [Source:HGNC Symbol;Acc:HGNC:4525] MLH mutl homolog 3 [Source:HGNC Symbol;Acc:HGNC:7128] CCDC coiled-coil domain containing 53 [Source:HGNC Symbol;Acc:HGNC:24256] GRB growth factor receptor bound protein 10 [Source:HGNC Symbol;Acc:HGNC:4564] EHD EH domain containing 3 [Source:HGNC Symbol;Acc:HGNC:3244] PREX phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 [Source:HGNC Symbol;Acc:HGNC:32594] GP glycoprotein VI platelet [Source:HGNC Symbol;Acc:HGNC:14388] HBS1L HBS1 like translational GTPase [Source:HGNC Symbol;Acc:HGNC:4834] HIPK homeodomain interacting protein kinase 2 [Source:HGNC Symbol;Acc:HGNC:14402] CBR1 873 carbonyl reductase 1 [Source:HGNC Symbol;Acc:HGNC:1548] BAZ1B 9031 bromodomain adjacent to zinc finger domain 1B [Source:HGNC Symbol;Acc:HGNC:961] SMIM small integral membrane protein 15 [Source:HGNC Symbol;Acc:HGNC:33861] UBE2K 3093 ubiquitin conjugating enzyme E2 K [Source:HGNC Symbol;Acc:HGNC:4914] PPFIA PTPRF interacting protein alpha 1 [Source:HGNC Symbol;Acc:HGNC:9245] MAP2K mitogen-activated protein kinase kinase 3 [Source:HGNC Symbol;Acc:HGNC:6843] PREPL 9581 prolyl endopeptidase-like [Source:HGNC Symbol;Acc:HGNC:30228] ARHGAP Rho GTPase activating protein 9 [Source:HGNC Symbol;Acc:HGNC:14130] OCIAD OCIA domain containing 2 [Source:HGNC Symbol;Acc:HGNC:28685] BRD bromodomain containing 9 [Source:HGNC Symbol;Acc:HGNC:25818] NUDT nudix hydrolase 5 [Source:HGNC Symbol;Acc:HGNC:8052] DEPDC1B DEP domain containing 1B [Source:HGNC Symbol;Acc:HGNC:24902] TNFRSF10C 8794 tumor necrosis factor receptor superfamily member 10c [Source:HGNC Symbol;Acc:HGNC:11906] CT45A cancer/testis antigen family 45, member A1 [Source:HGNC Symbol;Acc:HGNC:33267] PIM Pim-3 proto-oncogene, serine/threonine kinase [Source:HGNC Symbol;Acc:HGNC:19310] SLC24A solute carrier family 24 member 4 [Source:HGNC Symbol;Acc:HGNC:10978] TCERG transcription elongation regulator 1 [Source:HGNC Symbol;Acc:HGNC:15630] HCAR hydroxycarboxylic acid receptor 3 [Source:HGNC Symbol;Acc:HGNC:16824] UBP upstream binding protein 1 (LBP-1a) [Source:HGNC Symbol;Acc:HGNC:12507] BET Bet1 golgi vesicular membrane trafficking protein [Source:HGNC Symbol;Acc:HGNC:14562] GAB GRB2 associated binding protein 1 [Source:HGNC Symbol;Acc:HGNC:4066] ATG16L autophagy related 16 like 2 [Source:HGNC Symbol;Acc:HGNC:25464] GSK3B 2932 glycogen synthase kinase 3 beta [Source:HGNC Symbol;Acc:HGNC:4617] IQSEC IQ motif and Sec7 domain 1 [Source:HGNC Symbol;Acc:HGNC:29112] CAV2 858 caveolin 2 [Source:HGNC Symbol;Acc:HGNC:1528] DNM dynamin 3 [Source:HGNC Symbol;Acc:HGNC:29125] BIN bridging integrator 2 [Source:HGNC Symbol;Acc:HGNC:1053] IL6R 3570 interleukin 6 receptor [Source:HGNC Symbol;Acc:HGNC:6019] VPS13A vacuolar protein sorting 13 homolog A [Source:HGNC Symbol;Acc:HGNC:1908] EIF5A 1984 eukaryotic translation initiation factor 5A [Source:HGNC Symbol;Acc:HGNC:3300] NUFIP NUFIP2, FMR1 interacting protein 2 [Source:HGNC Symbol;Acc:HGNC:17634] PNRC proline rich nuclear receptor coactivator 2 [Source:HGNC Symbol;Acc:HGNC:23158] SMOX spermine oxidase [Source:HGNC Symbol;Acc:HGNC:15862] G6PD 2539 glucose-6-phosphate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4057] TWISTNB TWIST neighbor [Source:HGNC Symbol;Acc:HGNC:18027] MLXIP MLX interacting protein [Source:HGNC Symbol;Acc:HGNC:17055] DCAF DDB1 and CUL4 associated factor 5 [Source:HGNC Symbol;Acc:HGNC:20224] DDX19A DEAD-box helicase 19A [Source:HGNC Symbol;Acc:HGNC:25628] MYO1F 4542 myosin IF [Source:HGNC Symbol;Acc:HGNC:7600] MRPL mitochondrial ribosomal protein L43 [Source:HGNC Symbol;Acc:HGNC:14517] C6orf chromosome 6 open reading frame 25 [Source:HGNC Symbol;Acc:HGNC:13937] TMEM transmembrane protein 147 [Source:HGNC Symbol;Acc:HGNC:30414] BCAS breast carcinoma amplified sequence 3 [Source:HGNC Symbol;Acc:HGNC:14347] TRIB tribbles pseudokinase 1 [Source:HGNC Symbol;Acc:HGNC:16891] KIF2A 3796 kinesin heavy chain member 2A [Source:HGNC Symbol;Acc:HGNC:6318] TGFB transforming growth factor beta 1 [Source:HGNC Symbol;Acc:HGNC:11766] SUPT16H SPT16 homolog, facilitates chromatin remodeling subunit [Source:HGNC Symbol;Acc:HGNC:11465] ERP endoplasmic reticulum protein 44 [Source:HGNC Symbol;Acc:HGNC:18311] MYZAP myocardial zonula adherens protein [Source:HGNC Symbol;Acc:HGNC:43444] ARID1A 8289 AT-rich interaction domain 1A [Source:HGNC Symbol;Acc:HGNC:11110] TIMM translocase of inner mitochondrial membrane 10 homolog (yeast) [Source:HGNC Symbol;Acc:HGNC:11814] TRAPPC6B trafficking protein particle complex 6B [Source:HGNC Symbol;Acc:HGNC:23066] NOP NOP16 nucleolar protein [Source:HGNC Symbol;Acc:HGNC:26934] CD CD96 molecule [Source:HGNC Symbol;Acc:HGNC:16892] HAL 3034 histidine ammonia-lyase [Source:HGNC Symbol;Acc:HGNC:4806] SSX SSX family member 2 [Source:HGNC Symbol;Acc:HGNC:11336] 3.8 2