Figure S1. Localization of GiOR-1 with truncated N-terminal domain of 29 amino acid residues (ΔL-GiOR-1-HA).

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1 Figure S1. Localization of GiOR-1 with truncated N-terminal domain of 29 amino acid residues (ΔL-GiOR-1-HA). A L-GiOR-1-HA Tom40 DAPI merge DIC B LYS CYT ORG ORG Trypsin L-GiOR-1-HA

2 Figure S2A. Phylogeny of hydrogenases, Hyd-CPR fusion proteins and Nar1 Mastigamoeba balamuthi H Blastocystis hominis H 100/1 Trichomonas vaginalis F Trichomonas vaginalis F 68 /1 Carpediemonas membranifera F Chilomastix cuspidata F Dysnectes brevis F Trimastix PCT F Hydrogenases and 75 Trichomonas vaginalis H Hyd-CPR fusion proteins /1 100 /1 Trichomonas vaginalis H Trichomonas vaginalis H HYD FMN FAD 66 /1 Chlamydomonas reinhardtii H Naegleria gruberi H HYD 88/ 73/ Entamoeba histolytica H Giardia intestinalis H Spironucleus salmonicida H Spironucleus salmonicida H Encephalitozoon cuniculi 100/1 Chlamydomonas reinhardtii Arabidopsis thaliana 50/ 0.94 Mastigamoeba balamuthi 62 /0.58 Dictyostelium discoideum Tetrahymena thermophila Homo sapiens Saccharomyces cerevisiae Monosiga brevicollis Naegleria gruberi Cryptosporidium parvum Nar1 Blastocystis hominis Entamoeba histolytica 58/ 0.64 Carpediemonas membranifera Chilomastix cuspidata Dysnectes brevis 99/1 Giardia intestinalis 92/1 87/1 Spironucleus vortens Trepomonas Sp Spironucleus salmonicida 98/1 Bodo saltans 97/1 Leishmania major Trypanosoma brucei Trichomonas vaginalis 0.6 HYD

3 Figure S2B. Comparison of HC1 domains among hydrogenases (Hyd), hydrogenases that are fused with CPR (Hyd-CPR), and Nar1 components of CIA pathway. HC1 Naegleria gruberi Trichomonas vaginalis Giardia intestinalis Spironucleus salmonicida Blastocystis hominis Entamoeba histolytica Trimastix marina PCT Trichomonas vaginalis Trichomonas vaginalis Carpediemonas membranifera Chilomastix cuspidata Naegleria gruberi Bodo saltans Trichomonas vaginalis Carpediemonas membranifera Chilomastix cuspidata Dysnectes brevis Giardia intestinalis Spironucleus salmonicida Trepomonas PC1 Blastocystis hominis Entamoeba histolytica Saccharomyces cerevisiae MYTSCCPGWVNLVESDFPE - I I PNVSSCKSPQQMLGS- VI RN MFTSCCPAWVNYVEKSDPS -LI PHLSSCRSPMSMLSS- VI KN VFTSCCPAWVNLCEMKYPE - LLPQVSTAKSCVAMVATL- VKR VFTSCCPAWVNLCERKYPQ- LLGRLSTAKSPHGSI CT- LI KR MFTSCCPGWVNMAEKCYPE- LI PNLSSCRSPHMMLGSC- VKT AI TSCCPGWLEYLEKKYPD- LI PRVSTARSPI ACLAGC- I KN MFTSCCPGWI NLLEQKYPE - FI PNVSSCKSPQQMLGA- VI KT - FTSWCPAWVNFVEKLHPE -LIPNL STVKSPHI L LSV- I I RN MI TSS CPSWVNFI EKI FPD- LTENL STTMSPHI I TGS- LI KN MFTSCCPAWI NNI ETRFPD- YLDNLSTARSPMMMMGS- VVKT MFTSCCPGWI NFVEQEAPD- FI PNLSSCKSPMGMQSAL- LRT VFASACPGWI CYAEKTQPE - I I PSI STVKSPQQI MGT- FVKK MI VSS CPGWVCYCEKQYPE- LLPHLSRVMSPQGI AGS- LLKR - MI SRCAGSVVYYERKTS- - YADHLAQI KPYPQLYAMYEKKI LVVSS CPGFVCVCEKQNPE - VLPKMSRVGSPMAYTAA LFVSACPGWVCLAQKRYPD- TVARLARAQSSMTVFGTALLAR RI VTVCPAVRLYI GKRGPS -LIPHLDNTPSPTEI AGHGL AI I THCPAVRLFI TKRNRE- LI SYI VSTASPMELFGASYCGI --I THCPAVRLYI LKKKPE -LYKYVNTVGSMLEFQ LI VSHCPAAKLFLNKKDS S -LSKFFSKSASPLEI FFS FI TSNCPGWVCYAEKKTHA- LVPFLSTTKSAQQI MGS- LLRK - LTST CPGWVCYSEKMQGKWMFEYMSKVASSMTI AGMI - MKK LLSAVCPGFLI YTEKTKPQ- LVPMLLNVKSPQQI TGS- LI RA Hyd Hyd-CPR Nar1

4 Figure S3. Distribution of Tah18, Dre2, RNR1 and RNR2 proteins in Metamonada and various anaerobes. Tah18 Dre2 RNR1 RNR2 N. gruberi T. brucei B. saltans T.marina PCT T. vaginalis C. membranifera E. cyprinoides CLO NY171 C. cuspidata Excavata Anaerobes G. intestinalis S. salmonicida S. vortens Trepomonas PC1 B. hominis E. histolytica M. balamuthi D. dictyostelium D. brevis Amoebas

5 Figure Legends Figure S1. Localization of GiOR-1 with truncated N-terminal domain of 29 amino acid residues (ΔL-GiOR-1-HA). (A) Immunofluorescence microscopy revealed that ΔL-GiOR-1- HA localizes to mitosomes. ΔL-GiOR-1-HA was detected using anti hemagglutinin (HA) antibody (green). Tom40 was detected as mitosomal marker protein using polyclonal rabbit anti-tom40 antibody (red). Nuclei were visualized by DAPI (4,6-Diamidine-2 -phenylindole dihydrochloride). DIC, differential interference contrast. (B) Alternatively, the cellular localization of ΔL-GiOR-1-HA was investigated using western blot analysis of cellular fractions (LYS, cell lysate; CYT, the cytosol; ORG, the organellar fraction) and protein protection assay (treatment of the organellar fraction with 200 μg/ml of trypsin for 10 min at 37 C). Figure S2. Phylogeny of hydrogenase like domain containing proteins A. Maximum likelihood-based phylogeny of Nar1 and hydrogenase domain-containing proteins in eukaryotes. Hydrogenases cluster together with the CPR-hydrogenase fusion from metamonads. Numbers indicate statistical support in the form of bootstrap values or PP values. The scale bar represents the estimated number of amino acid substitutions per site. Only bootstrap support values greater than 50 and PP values greater than 0.5 are shown. Green indicates Viridiplantae, red Opisthokonta, yellow the SAR supergroup, brown Amoebozoa, and blue Excavata. H, only hydrogenase domain-containing protein; F, CPRhydrogenase fusion protein. B. Alignment of HC1 site of CPR-hydrogenase fusion proteins in metamonads with representative protein sequences for hydrogenase and nar1 protein. The HC1 motif is highlighted.

6 Figure S3. Distribution of Tah18, Dre2, RNR1 and RNR2 proteins in Metamonada and various anaerobes. A function in the synthesis of diferric-tyrosyl radical (Fe(III)2-Y ) cofactor of ribonucleotide reductase (RNR) was recently suggested for Tah18 and Dre2 (17). Hence, we searched to determine whether genes for RNR proteins are missing in organisms lacking Tah18-Dre2 proteins. Nevertheless, we failed to find any such correlation to support this hypothesis. RNR1, Ribonucleotide reductase 1; RNR2, Ribonucleotide reductase 2. Black shading, an ortholog is present; White, an ortholog is absent from the genomic database; Grey, an ortholog is absent from a given EST database.

7 Table S1. Analysis of immunoprecipitated proteins by mass spectrometry Identified proteins Acc. Number Molecular weight Protein identification probability Total unique spectra count GiOR1 Cia2 CBirA CBirA CBirA CBirA Wt Wt GiOR1 Cia2 CBirA CBirA CBirA CBirA Wt Wt Nitric oxide synthase, inducible location=glchr04: ( ) length=604 GL50803_ kda 100% 7 Chaperone protein DnaK HSP70 location=glchr03: ( ) length=640 GL50803_ kda 100% 9 Chaperonin 60 location=glchr02: ( ) length=547 GL50803_ kda 100% 8 Cysteine desulfurase location=glchr03: (+) length=433 GL50803_ kda 100% 4 Alpha mitochondrial protein peptidase like protein location=glchr01: ( GL50803_ kda 100% 3 Glutaredoxin related protein location=glchr01: (+) length=202 GL50803_ kda 100% 3 Hypothetical protein location=glchr05: ( ) length=286 GL50803_ kda 100% 2 Hypothetical protein location=glchr01: (+) length=278 GL50803_ kda 100% 2 Hypothetical protein location=glchr04: ( ) length=197 GL50803_ kda 100% 2 [2Fe 2S] ferredoxin location=glchr02: ( ) length=133 GL50803_ kda 100% 2 Hypothetical protein location=glchr03: ( ) length=125 GL50803_ kda 100% 2 Hypothetical protein location=glchr01: (+) length=361 GL50803_ kda 100% 96% 2 1 HesB domain containing protein location=glchr01: ( ) length=131 GL50803_ kda 100% 1 Beta adaptin location=glchr05: ( ) length=1132 GL50803_ kda 100% 1 Hypothetical protein location=ch991767: (+) length=1095 GL50803_ kda 100% 1 Gamma giardin location=glchr03: ( ) length=311 GL50803_ kda 100% 1 Axonemal p66.0 location=glchr03: (+) length=552 GL50803_ kda 100% 1 Hypothetical protein location=glchr01: (+) length=745 GL50803_ kda 100% 1 Hypothetical protein location=glchr01: (+) length=745 GL50803_ kda 100% 1 Hypothetical protein location=glchr02: (+) length=464 GL50803_ kda 100% 1 Kinase, NEK location=glchr04: ( ) length=898 GL50803_ kda 100% 1 Hypothetical protein location=glchr05: ( ) length=264 GL50803_ kda 99% 1 Hypothetical protein location=glchr03: ( ) length=669 GL50803_ kda 99% 1 Hypothetical protein location=glchr02: (+) length=228 GL50803_ kda 99% 1 Hypothetical protein location=glchr04: (+) length=472 GL50803_ kda 98% 1 Hypothetical protein location=glchr05: (+) length=805 GL50803_ kda 95% 1 Protein required for cell viability location=glchr05: (+) length=145 GL50803_ kda 100% 7 Coiled coil protein location=glchr04: ( ) length=1675 GL50803_ kda 100% 7 ELKS location=glchr01: (+) length=924 GL50803_ kda 100% 100% 1 4 Nuclear ATP/GTP binding protein location=glchr02: ( ) length=1422 GL50803_ kda 100% 3 MDR type permease location=glchr05: ( ) length=666 GL50803_ kda 100% 3 Hypothetical protein location=glchr05: (+) length=1471 GL50803_ kda 100% 100% 1 2 Hypothetical protein location=glchr03: (+) length=702 GL50803_ kda 100% 2 Hypothetical protein location=glchr02: ( ) length=252 GL50803_ kda 100% 2 Hypothetical protein location=glchr05: ( ) length=772 GL50803_ kda 100% 2 Hypothetical protein location=glchr02: (+) length=2292 GL50803_ kda 100% 1 hypothetical protein location=glchr05: ( ) length=255 GL50803_ kda 96% 1 Kinase, NEK location=glchr04: ( ) length=823 GL50803_ kda 100% 1 Potassium transporting ATPase alpha chain 1 location=glchr02: (+) GL50803_ kda 100% 1 Rac/Rho like protein location=glchr03: (+) length=218 GL50803_ kda 96% 1 Hypothetical protein location=glchr01: (+) length=199 GL50803_ kda 96% 1 Hypothetical protein location=glchr04: ( ) length=1261 GL50803_ kda 96% 1 Protein 21.1 location=glchr05: ( ) length=330 GL50803_ kda 100% 95% 2 1 Coiled coil protein location=glchr02: (+) length=2275 GL50803_ kda 100% 99% 1 1 Kinase, NEK location=glchr03: ( ) length=287 GL50803_ kda 96% 1 DNA directed RNA polymerase RPB3 location=glchr05: (+) GL50803_ kda 96% 1 Manganese dependent inorganic pyrophosphatase, putative GL50803_ kda 98% 1 Vacuolar ATP synthase subunit H location=glchr01: (+) length=530 GL50803_ kda 100% 1 Centromere/microtubule binding protein CBF5 location=glchr05: (+) GL50803_ kda 99% 1 H SHIPPO 1 location=glchr05: ( ) length=615 GL50803_ kda 99% 1 Alpha 11 giardin location=glchr05: ( ) length=307 GL50803_ kda 96% 1

8 Sec23 location=glchr02: (+) length=861 GL50803_ kda 96% 1 Kinase, CMGC CDK location=glchr02: (+) length=340 GL50803_ kda 96% 1 Hypothetical protein location=glchr05: ( ) length=974 GL50803_ kda 95% 1 Nucleolar GTP binding protein 1, putative location=glchr04: ( ) GL50803_ kda 99% 1 Phosphatase location=glchr01: ( ) length=310 GL50803_ kda 100% 1 Kinase, CAMK CAMKL location=glchr03: ( ) length=432 GL50803_ kda 95% 96% 1 1 UTP glucose 1 phosphate uridylyltransferase location=glchr04: (+) GL50803_ kda 100% 1 MCT 1 protein like protein location=glchr05: (+) length=237 GL50803_ kda 99% 1 Kinase, NEK location=glchr05: ( ) length=349 GL50803_ kda 96% 1 Nucleoside diphosphate kinase location=glchr03: ( ) length=387 GL50803_ kda 96% 1 Hypothetical protein location=glchr05: ( ) length=198 GL50803_ kda 96% 1 Phosphatase location=glchr02: ( ) length=385 GL50803_ kda 96% 1 Transglutaminase/protease, putative location=glchr05: ( ) GL50803_ kda 96% 1 Phosphatidate cytidylyltransferase location=glchr03: ( ) length=376 GL50803_ kda 95% 1 Phosphorylated CTD interacting factor PCIF1 location=glchr04: ( ) GL50803_ kda 96% 1 IFT complex B location=glchr03: ( ) length=672 GL50803_ kda 96% 1 Hypothetical protein location=glchr04: ( ) length=605 GL50803_ kda 99% 1 Clathrin heavy chain location=glchr04: (+) length=1871 GL50803_ kda 100% 1 Hypothetical protein location=glchr03: ( ) length=848 GL50803_ kda 96% 1 Spindle pole protein, putative location=glchr05: ( ) length=554 GL50803_ kda 96% 1 L asparaginase location=glchr04: (+) length=400 GL50803_ kda 96% 1 Kinase, NEK location=glchr04: (+) length=386 GL50803_ kda 96% 1 Translation initiation factor 2 gamma subunit location=glchr04: (+) GL50803_ kda 96% 1 Translation initiation factor eif 4A, putative location=glchr01: ( ) GL50803_ kda 96% 95% 1 1 Protein location=glchr05: (+) length=748 GL50803_ kda 99% 100% 100% Ser/Thr phosphatase 2C, putative location=glchr02: ( ) GL50803_ kda 96% 1 La ribonucleoprotein, putative location=glchr05: ( ) length=348 GL50803_ kda 100% 1 Protein 21.1 location=glchr03: (+) length=1457 GL50803_ kda 100% 100% 7 1 Trichohyalin location=glchr05: ( ) length=1414 GL50803_ kda 100% 1 Kinase, NEK location=glchr03: (+) length=650 GL50803_ kda 100% 100% 1 1 Protein 21.1 location=glchr04: ( ) length=1500 GL50803_ kda 100% 99% 6 1 Hypothetical protein location=glchr05: ( ) length=342 GL50803_ kda 99% 100% 95% Alcohol dehydrogenase lateral transfer candidate location=glchr04: GL50803_ kda 96% 1 Beta giardin location=glchr04: (+) length=272 GL50803_ kda 100% 95% 2 1 Hypothetical protein location=glchr02: (+) length=254 GL50803_ kda 100% 97% S proteasome regulatory subunit, putative location=glchr01: (+) GL50803_ kda 96% 1 Type II inositol 1,4,5 trisphosphate 5 phosphatase precursor GL50803_ kda 100% 1 Hypothetical protein location=glchr02: ( ) length=493 GL50803_ kda 96% 1 Uridine kinase location=glchr05: ( ) length=682 GL50803_ kda 96% 1 Hypothetical protein location=glchr04: (+) length=480 GL50803_ kda 96% 1 hypothetical protein location=glchr04: (+) length=853 GL50803_ kda 96% 1 Hypothetical protein location=glchr04: ( ) length=308 GL50803_ kda 95% 1 Uridine kinase location=glchr03: (+) length=582 GL50803_ kda 96% 1 Kinase, NEK location=glchr05: ( ) length=493 GL50803_ kda 100% 2 Long chain fatty acid CoA ligase 5 location=glchr05: (+) GL50803_ kda 100% 2 Histone H2B location=glchr05: (+) length=130 GL50803_ kda 95% 1 Kinase, CMGC DYRK location=glchr01: ( ) length=860 GL50803_ kda 96% 1 DNA topoisomerase III location=glchr04: (+) length=973 GL50803_ kda 96% 1 Phosphoenolpyruvate carboxykinase location=glchr03: ( ) GL50803_ kda 100% 3 Hypothetical protein location=glchr04: (+) length=125 GL50803_ kda 95% 1 Peptide methionine sulfoxide reductase msrb location=glchr05: (+) GL50803_ kda 95% 1 Dynein intermediate chain location=glchr02: (+) length=757 GL50803_ kda 100% 1 CAMP dependent protein kinase regulatory chain location=glchr05: GL50803_ kda 100% 3 Ribosome biogenesis protein BMS1 location=glchr03: (+) GL50803_ kda 100% 1 hypothetical protein location=glchr04: ( ) length=872 GL50803_ kda 100% 100% 99% 95%

9 Histidyl trna synthetase location=glchr01: ( ) length=961 GL50803_ kda 96% 1 70 kda peptidylprolyl isomerase, putative location=glchr04: ( ) GL50803_ kda 100% 2 Nitroreductase Fd NR2 location=glchr01: (+) length=264 GL50803_ kda 96% 1 Hypothetical protein location=glchr02: ( ) length=319 GL50803_ kda 99% 1 Aminoacyl histidine dipeptidase location=glchr05: (+) length=506 GL50803_ kda 100% 1 Kinase, STE STE20 location=ch991761: (+) length=423 GL50803_ kda 96% 1 Farnesyl diphosphate synthase location=glchr04: ( ) length=407 GL50803_ kda 96% 1 Protein 21.1 location=glchr05: ( ) length=681 GL50803_ kda 100% 2 A type flavoprotein lateral transfer candidate location=glchr04: ( ) GL50803_ kda 100% 2 Protein 21.1 location=glchr03: ( ) length=760 GL50803_ kda 100% 100% 100% Protein phosphatase 2A regulatory subunit, putative location=glchr04: GL50803_ kda 100% 2 Rab2a location=glchr04: ( ) length=214 GL50803_ kda 100% 100% 1 1 Hypothetical protein location=glchr02: ( ) length=431 GL50803_ kda 100% 99% 1 1 Kinesin 13 location=glchr03: ( ) length=714 GL50803_ kda 100% 1 Hypothetical protein location=glchr05: (+) length=588 GL50803_ kda 100% 100% 1 1 Kinase, NEK frag location=glchr02: (+) length=706 GL50803_ kda 100% 2 hypothetical protein location=glchr05: ( ) length=1529 GL50803_ kda 100% 1 Kinase, CAMK CAMKL location=glchr05: (+) length=403 GL50803_ kda 96% 1 Phosphorylase B kinase gamma catalytic chain location=glchr04: ( ) GL50803_ kda 96% 1 Hypothetical protein location=glchr04: (+) length=875 GL50803_ kda 95% 1 Protein 21.1 location=glchr04: ( ) length=785 GL50803_ kda 95% 1 Ribosomal protein S29A location=glchr05: (+) length=137 GL50803_ kda 100% 1 TCP 1 chaperonin subunit alpha location=glchr04: (+) length=550 GL50803_ kda 99% 1 Coiled coil protein location=ch991767: (+) length=1685 GL50803_ kda 100% 2 TCP 1 chaperonin subunit beta location=glchr02: ( ) length=525 GL50803_ kda 100% 2 Hypothetical protein location=glchr01: (+) length=528 GL50803_ kda 100% 1 Protein F10G7.1 location=glchr04: (+) length=826 GL50803_ kda 100% 2 Dual specificity phosphatase Cdc25, putative location=glchr03: (+) GL50803_ kda 95% 100% 1 1 Dipeptidyl peptidase III location=glchr01: (+) length=694 GL50803_ kda 100% 1 Hypothetical protein location=ch991767: ( ) length=1262 GL50803_ kda 96% 1 Prefoldin subunit 3, putative location=glchr01: (+) length=192 GL50803_ kda 100% 1 Nucleolar protein NOP5 location=glchr05: (+) length=613 GL50803_ kda 100% 100% 100% Ubiquitin conjugating enzyme E1 location=glchr05: ( ) GL50803_ kda 100% 2 DEAD box RNA helicase Vasa location=glchr05: (+) length=449 GL50803_ kda 100% 1 TCP 1 chaperonin subunit theta location=glchr02: ( ) length=563 GL50803_ kda 100% 1 Alcohol dehydrogenase lateral transfer candidate location=glchr03: GL50803_ kda 100% 1 Ribosomal protein L7 location=glchr04: (+) length=235 GL50803_ kda 100% 100% 1 1 Kinase, NEK location=glchr05: ( ) length=288 GL50803_ kda 100% 3 Hypothetical protein location=glchr05: ( ) length=262 GL50803_ kda 100% 95% 3 1 Proteasome subunit beta type 1 location=glchr05: ( ) length=254 GL50803_ kda 100% 2 Nucleolar protein NOP5 location=glchr01: ( ) length=498 GL50803_ kda 100% 2 Coiled coil protein location=glchr05: ( ) length=1652 GL50803_ kda 100% 1 Metalloprotease, insulinase family location=glchr02: (+) GL50803_ kda 100% 2 Hypothetical protein location=glchr02: ( ) length=237 GL50803_ kda 100% 1 Translation elongation factor location=glchr01: ( ) length=220 GL50803_ kda 100% 2 Hypothetical protein location=glchr05: ( ) length=250 GL50803_ kda 100% 2 Hypothetical protein location=glchr03: (+) length=213 GL50803_ kda 100% 1 hypothetical protein location=glchr03: ( ) length=4360 GL50803_ kda 96% 1 20S proteasome alpha subunit 5 location=glchr02: ( ) length=245 GL50803_ kda 100% 2 Serine/threonine protein phosphatase PP X isozyme 2 location=glchr02: GL50803_ kda 100% 1 Ubiquitin carboxyl terminal hydrolase 4 location=glchr05: ( ) GL50803_ kda 100% 2 Hypothetical protein location=glchr03: ( ) length=204 GL50803_ kda 100% 1 Kinase, NEK location=glchr04: ( ) length=584 GL50803_ kda 100% 2 Nucleolar GTP binding protein 2 location=glchr05: ( ) length=737 GL50803_ kda 100% 2 Hypothetical protein location=glchr03: (+) length=330 GL50803_ kda 100% 100% 1 1

10 Glutaminyl trna synthetase location=glchr05: ( ) length=702 GL50803_ kda 100% 1 Rab11 location=glchr05: ( ) length=216 GL50803_ kda 96% 100% 1 3 Alanine aminotransferase, putative location=glchr03: (+) length=521 GL50803_ kda 100% 3 Hypothetical protein location=glchr04: ( ) length=845 GL50803_ kda 100% 2 Prolyl trna synthetase location=glchr04: (+) length=542 GL50803_ kda 100% 1 Hypothetical protein location=glchr05: (+) length=959 GL50803_ kda 99% 100% 1 1 TBP interacting protein TIP49 location=glchr02: (+) length=468 GL50803_ kda 100% 3 Hypothetical protein location=glchr05: (+) length=1236 GL50803_ kda 100% 100% 99% 2 2 Aldose reductase location=glchr02: (+) length=313 GL50803_ kda 100% 2 Hypothetical protein location=glchr02: (+) length=271 GL50803_ kda 100% 2 Serine/threonine protein phosphatase 7 location=glchr05: (+) GL50803_ kda 100% 2 Hypothetical protein location=glchr05: (+) length=276 GL50803_ kda 100% 100% 100% hypothetical protein location=glchr02: ( ) length=423 GL50803_ kda 100% 2 Kinase, NEK location=glchr02: (+) length=285 GL50803_ kda 100% 2 Hypothetical protein location=glchr05: (+) length=888 GL50803_ kda 100% 100% 100% Dual specificity protein phosphatase CDC14A location=glchr04: (+) GL50803_ kda 100% 99% 1 1 Hypothetical protein location=glchr04: (+) length=460 GL50803_ kda 96% 100% 1 2 Hypothetical protein location=glchr05: ( ) length=123 GL50803_ kda 100% 1 Hypothetical protein location=glchr02: (+) length=260 GL50803_ kda 100% 3 Fibrillarin like pre rrna processing protein Narcisi et al location=glchr05: GL50803_ kda 100% 3 hypothetical protein location=glchr04: (+) length=1296 GL50803_ kda 100% 3 Kinase, CMGC MAPK location=glchr05: ( ) length=360 GL50803_ kda 100% 3 TCP 1 chaperonin subunit eta location=glchr05: ( ) length=595 GL50803_ kda 100% 1 S adenosylmethionine synthetase location=glchr01: (+) GL50803_ kda 100% 2 Protein location=glchr05: ( ) length=1044 GL50803_ kda 100% 100% 4 1 Kinase, NEK location=glchr03: (+) length=376 GL50803_ kda 100% 100% 1 1 Kinase, NEK location=glchr04: (+) length=405 GL50803_ kda 100% 1 Threonyl trna synthetase location=glchr05: (+) length=695 GL50803_ kda 100% 3 Kinesin 14 location=glchr01: ( ) length=1172 GL50803_ kda 100% 5 hypothetical protein location=glchr04: ( ) length=1003 GL50803_ kda 100% 100% 100% Bip location=glchr04: (+) length=677 GL50803_ kda 100% 100% 100% Hypothetical protein location=glchr05: (+) length=1904 GL50803_ kda 100% 3 Ribosomal protein S7 location=glchr05: ( ) length=190 GL50803_ kda 100% 2 Coiled coil protein location=ch991776: (+) length=1086 GL50803_ kda 100% 5 Plasma membrane calcium transporting ATPase 2 location=glchr05: GL50803_ kda 100% 100% 3 5 NADH oxidase lateral transfer candidate location=glchr01: ( ) GL50803_ kda 100% 100% 1 3 Hypothetical protein location=glchr04: (+) length=567 GL50803_ kda 100% 4 Phosphomannomutase 2 location=glchr04: ( ) length=655 GL50803_ kda 100% 3 FKBP type peptidyl prolyl cis trans isomerase location=glchr01: ( ) GL50803_ kda 100% 3 Alcohol dehydrogenase 3 lateral transfer candidate location=glchr03: GL50803_ kda 100% 1 Hexose transporter location=glchr03: ( ) length=512 GL50803_ kda 95% 95% 1 1 Protein 21.1 location=glchr04: (+) length=723 GL50803_ kda 95% 1 Hypothetical protein location=glchr01: ( ) length=257 GL50803_ kda 95% 96% 96% 97% Glucokinase location=glchr05: (+) length=343 GL50803_ kda 100% 2 U3 small nucleolar RNA associated protein 11, putative location=glchr04: GL50803_ kda 96% 1 Kinase, NEK location=glchr03: ( ) length=733 GL50803_ kda 96% 1 Cysteinyl trna synthetase location=glchr04: ( ) length=645 GL50803_ kda 96% 1 Vacuolar ATP synthase subunit C location=glchr02: (+) length=494 GL50803_ kda 96% 1 Phosphoglycolate phosphatase location=glchr05: (+) length=228 GL50803_ kda 95% 1 Ubiquitin conjugating enzyme E2 17 kda location=glchr02: (+) GL50803_ kda 95% 1 Hypothetical protein location=glchr04: ( ) length=141 GL50803_ kda 95% 95% 1 1 FKBP type peptidyl prolyl cis trans isomerase location=glchr01: ( ) GL50803_ kda 100% 1 Hypothetical protein location=glchr02: (+) length=81 GL50803_ kda 95% 1 GTP binding protein location=glchr05: ( ) length=507 GL50803_ kda 100% 1 Hypothetical protein location=glchr05: (+) length=445 GL50803_ kda 99% 1

11 Spindle pole protein, putative location=glchr03: ( ) length=693 GL50803_ kda 99% 1 Hypothetical protein location=glchr02: (+) length=153 GL50803_ kda 100% 1 Arsenical pump driving ATPase location=glchr03: ( ) length=354 GL50803_ kda 96% 1 Hypothetical protein location=glchr05: (+) length=284 GL50803_ kda 98% 96% 95% 100% Hypothetical protein location=glchr01: ( ) length=140 GL50803_ kda 100% 1 Alanyl trna synthetase location=glchr04: ( ) length=969 GL50803_ kda 100% 1 Protein 21.1 location=glchr05: (+) length=925 GL50803_ kda 99% 1 L serine dehydratase location=glchr03: (+) length=516 GL50803_ kda 96% 1 TBP interacting protein TIP49 location=glchr04: ( ) length=483 GL50803_ kda 98% 1 Protein 21.1 location=glchr05: ( ) length=1873 GL50803_ kda 100% 1 LEK1 location=glchr05: (+) length=716 GL50803_ kda 100% 1 Hypothetical protein location=glchr05: ( ) length=483 GL50803_ kda 96% 1 Vacuolar protein sorting 26, putative location=glchr05: (+) GL50803_ kda 100% 1 Ubiquitin fusion degradation protein 1 location=glchr01: ( ) GL50803_ kda 96% 1 Spindle pole protein, putative location=glchr05: ( ) length=1032 GL50803_ kda 100% 1 organism=crap product=crap_aldoa_rabit GL50803_ kda 95% 1 Hypothetical protein location=glchr01: ( ) length=695 GL50803_ kda 100% 96% 100% Hypothetical protein location=glchr03: (+) length=599 GL50803_ kda 95% 1 Ripening regulated protein DDTFR19 location=glchr05: (+) GL50803_ kda 98% 100% 1 1 SnRNP Sm like protein, putative location=glchr04: (+) length=125 GL50803_ kda 100% 1 Hypothetical protein location=glchr03: ( ) length=223 GL50803_ kda 100% 1 Protein 21.1 location=glchr05: ( ) length=886 GL50803_ kda 100% 1 Hypothetical protein location=glchr05: (+) length=990 GL50803_ kda 100% 1 Hypothetical protein location=glchr05: ( ) length=1086 GL50803_ kda 100% 2 Polyadenylate binding protein, putative location=glchr01: ( ) GL50803_ kda 100% 1 ATP dependent RNA helicase location=glchr05: ( ) length=616 GL50803_ kda 100% 2 ATP dependent RNA helicase p47, putative location=glchr05: (+) GL50803_ kda 100% 2 ACTIN location=glchr02: ( ) length=375 GL50803_ kda 99% 100% 95% Ribosomal protein L7Ae location=glchr04: ( ) length=122 GL50803_ kda 100% 1 ARF GAP location=glchr05: ( ) length=398 GL50803_ kda Hypothetical protein location=glchr04: (+) length=331 GL50803_ kda 100% 2 Ribose 5 phosphate isomerase location=glchr03: ( ) length=145 GL50803_ kda 100% 1 Eukaryotic translation initiation factor 2 beta subunit location=glchr05: GL50803_ kda 100% 2 Hypothetical protein location=glchr03: (+) length=275 GL50803_ kda 95% 100% 95% NADH oxidase location=glchr05: (+) length=429 GL50803_ kda 100% 1 Low molecular weight protein tyrosine phosphatase location=glchr05: GL50803_ kda 100% 2 NADPH oxidoreductase, putative location=glchr05: ( ) length=164 GL50803_ kda 100% 1 Hypothetical protein location=glchr03: ( ) length=137 GL50803_ kda 100% 95% 1 1 Ribosomal protein L5 location=glchr02: (+) length=297 GL50803_ kda 100% 2 Ribosomal protein S27 location=glchr04: (+) length=124 GL50803_ kda 100% 2 Cathepsin B precursor location=glchr04: ( ) length=300 GL50803_ kda 95% 97% 1 1 Translation initiation inhibitor location=glchr02: (+) length=120 GL50803_ kda 100% 2 Ser/Thr protein kinase location=glchr04: ( ) length=869 GL50803_ kda 100% 100% Zinc finger domain location=glchr02: (+) length=128 GL50803_ kda 100% 100% 1 1 Hypothetical protein location=glchr05: (+) length=668 GL50803_ kda 100% 1 Hypothetical protein location=glchr01: (+) length=192 GL50803_ kda 100% 1 RNA binding protein, putative location=glchr05: (+) length=273 GL50803_ kda 100% 2 Heat shock protein, putative location=glchr04: (+) length=845 GL50803_ kda 100% 3 Hypothetical protein location=glchr05: ( ) length=288 GL50803_ kda 100% 2 Hypothetical protein location=glchr03: ( ) length=373 GL50803_ kda 100% 2 Cluster of SKD1 protein location=glchr05: (+) length=454 GL50803_ [2] 50 kda 100% 2 Topoisomerase II location=glchr05: (+) length=519 GL50803_ kda 100% 2 Threonine dehydratase lateral transfer candidate location=glchr04: ( ) GL50803_ kda 100% 1 CGI 35 protein, putative location=glchr01: (+) length=190 GL50803_ kda 100% 1 Kinesin 3 location=glchr04: (+) length=1026 GL50803_ kda 90% 100% 1 2

12 hypothetical protein location=glchr03: (+) length=144 GL50803_ kda 100% 2 Kinesin like protein location=glchr01: ( ) length=796 GL50803_ kda 100% 1 Hypothetical protein location=glchr04: ( ) length=177 GL50803_ kda 100% 1 Phosphatidylinositol 4 phosphate 5 kinase, putative location=glchr02: GL50803_ kda 100% 100% 6 1 Branched chain amino acid aminotransferase lateral transfer candidate GL50803_ kda 100% 100% 1 5 Hypothetical protein location=glchr04: ( ) length=150 GL50803_ kda 100% 1 Ribosomal protein L30 location=glchr05: (+) length=109 GL50803_ kda 100% 2 WD 40 repeat protein family location=glchr01: ( ) length=342 GL50803_ kda 96% 100% 1 2 Ribosomal protein L15 location=glchr02: (+) length=204 GL50803_ kda 100% 100% 100% 100% 100% Eukaryotic translation initiation factor 5A location=glchr05: (+) GL50803_ kda 100% 1 Heat shock protein HSP 90 alpha location=glchr05: (+) length=324 GL50803_ kda 100% 3 Ribosomal protein L44 location=glchr05: ( ) length=106 GL50803_ kda 100% 100% 96% 100% 100% Ribosomal protein L7a location=glchr03: (+) length=225 GL50803_ kda 99% 95% 100% 100% 95% Hypothetical protein location=glchr01: ( ) length=971 GL50803_ kda 100% 100% 1 1 Pyrophosphate fructose 6 phosphate 1 phosphotransferase alpha subunit GL50803_ kda 100% 2 Hypothetical protein location=glchr02: (+) length=479 GL50803_ kda 100% 100% 2 1 Hypothetical protein location=glchr04: ( ) length=680 GL50803_ kda 100% 3 Hypothetical protein location=glchr02: ( ) length=88 GL50803_ kda 100% 2 Liver stage antigen like protein location=glchr03: (+) length=1811 GL50803_ kda 100% 100% 100% 100% Ribosomal protein L9 location=glchr05: ( ) length=185 GL50803_ kda 100% 100% 100% Aminoacyl histidine dipeptidase location=glchr05: (+) length=524 GL50803_ kda 100% 8 Glutamate synthase location=glchr05: ( ) length=910 GL50803_ kda 100% 6 Ribosomal protein L13a location=glchr04: (+) length=197 GL50803_ kda 95% 100% 92% Ribosomal protein L32 location=glchr04: (+) length=136 GL50803_ kda 100% 2 Heat shock protein HSP 90 alpha location=glchr05: (+) length=376 GL50803_ kda 100% 4 Kinase, STE STE20 location=glchr03: ( ) length=527 GL50803_ kda 100% 3 Protein 21.1 location=glchr04: ( ) length=343 GL50803_ kda 100% 4 Chaperone protein DnaJ location=glchr05: ( ) length=409 GL50803_ kda 100% 4 Hypothetical protein location=glchr05: (+) length=367 GL50803_ kda 100% 1 Transketolase location=glchr03: ( ) length=719 GL50803_ kda 100% 3 Glucosamine 6 phosphate N acetyltransferase location=glchr05: ( ) GL50803_ kda 100% 2 Deoxyribose phosphate aldolase lateral transfer candidate location=glchr04: GL50803_ kda 96% 1 Dynamin location=glchr03: (+) length=732 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr04: (+) length=77 GL50803_ kda 100% 100% 100% methyl 5 thiazole monophosphate biosynthesis enzyme location=glchr02: GL50803_ kda 100% 2 Glycogen phosphorylase location=glchr05: (+) length=924 GL50803_ kda 100% 3 Ribokinase location=glchr01: (+) length=344 GL50803_ kda 100% 2 Ser/Thr protein kinase location=glchr02: (+) length=285 GL50803_ kda 100% 100% 1 2 Protein 21.1 location=glchr05: (+) length=1251 GL50803_ kda 100% 100% 2 1 Hypothetical protein location=glchr02: (+) length=782 GL50803_ kda 100% 2 Hypothetical protein location=glchr03: ( ) length=1025 GL50803_ kda 100% 100% 100% FixW protein, putative location=glchr05: ( ) length=133 GL50803_ kda 100% 2 Adenylate kinase location=glchr01: ( ) length=190 GL50803_ kda 100% 2 Giardia trophozoite antigen GTA 2 location=glchr03: ( ) length=236 GL50803_ kda 100% 96% 99% Ribosomal protein L37 location=glchr02: ( ) length=89 GL50803_ kda 100% 100% 100% 100% 100% Dynein light chain location=glchr05: ( ) length=144 GL50803_ kda 100% 2 Hypothetical protein location=glchr03: ( ) length=1437 GL50803_ kda 100% 2 Hypothetical protein location=glchr04: ( ) length=131 GL50803_ kda 100% 100% 1 1 Ubiquitin conjugating enzyme E2 17 kda 3 location=ch991761: (+) GL50803_ kda 100% 1 Hypothetical protein, similar to AMMECR1 location=glchr05: ( ) GL50803_ kda 100% 1 Ribosomal protein L36 1 location=glchr05: (+) length=90 GL50803_ kda 100% 100% 1 2 Ribosomal protein L35 location=glchr05: ( ) length=152 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr01: ( ) length=973 GL50803_ kda 100% 100% 100% 100% Hypothetical protein location=glchr05: (+) length=1330 GL50803_ kda 100% 100% 100% Hypothetical protein location=glchr01: (+) length=569 GL50803_ kda 100% 100% 100% 6 4 1

13 Ribosomal protein L35a location=glchr01: (+) length=123 GL50803_ kda 96% 100% 100% Hypothetical protein location=ch991767: (+) length=386 GL50803_ kda 100% 100% 100% Hypothetical protein location=glchr03: ( ) length=383 GL50803_ kda 100% 7 Protein 21.1 location=glchr05: ( ) length=1052 GL50803_ kda 100% 100% 100% 100% Spindle pole protein, putative location=glchr04: ( ) length=623 GL50803_ kda 100% 100% 2 1 hypothetical protein location=glchr03: (+) length=491 GL50803_ kda 95% 100% 1 7 Hypothetical protein location=glchr05: (+) length=507 GL50803_ kda 100% 100% 100% Pyruvate flavodoxin oxidoreductase location=glchr05: ( ) GL50803_ kda 100% 6 Hypothetical protein location=glchr04: (+) length=766 GL50803_ kda 100% 100% 2 1 Protein 21.1 location=glchr05: (+) length=623 GL50803_ kda 100% 100% 1 6 Ribosomal protein S15 location=glchr05: ( ) length=145 GL50803_ kda 100% 100% 2 1 Phosphatidylinositol transfer protein alpha isoform location=glchr02: GL50803_ kda 100% 8 Ribosomal protein L11 location=glchr05: ( ) length=173 GL50803_ kda 95% 95% 100% 100% 97% Pyruvate kinase location=glchr03: ( ) length=654 GL50803_ kda 100% 100% 1 3 Elongation factor 1 gamma location=glchr04: ( ) length=402 GL50803_ kda 98% 100% 1 4 Vacuolar ATP synthase subunit B location=glchr04: (+) length=497 GL50803_ kda 100% 6 ATP dependent RNA helicase like protein location=glchr05: (+) GL50803_ kda 100% 6 Vacuolar ATP synthase catalytic subunit A location=glchr03: (+) GL50803_ kda 100% 6 Protein 21.1 location=glchr03: ( ) length=1156 GL50803_ kda 100% 2 Ubiquitin location=glchr05: (+) length=82 GL50803_ kda 100% 100% 4 1 Hypothetical protein location=glchr01: (+) length=164 GL50803_ kda 100% 3 Hypothetical protein location=glchr04: ( ) length=739 GL50803_ kda 100% 3 Ribosomal protein L24 location=glchr05: (+) length=189 GL50803_ kda 100% 100% 2 1 2,3 bisphosphoglycerate independent phosphoglycerate mutase GL50803_ kda 100% 4 Dynein light chain location=glchr01: ( ) length=89 GL50803_ kda 100% 3 20S proteasome alpha subunit 4 location=glchr05: (+) length=214 GL50803_ kda 100% 2 Cytidine deaminase location=glchr01: (+) length=134 GL50803_ kda 100% 3 Kinase, NEK location=glchr02: (+) length=777 GL50803_ kda 100% 4 Hypothetical protein location=glchr05: (+) length=171 GL50803_ kda 100% 2 Hypothetical protein location=glchr01: ( ) length=621 GL50803_ kda 100% 5 Hypothetical protein location=glchr01: (+) length=176 GL50803_ kda 100% 96% 2 1 GTP binding protein Sar1 location=glchr02: ( ) length=191 GL50803_ kda 100% 1 TCP 1 chaperonin subunit epsilon location=glchr01: ( ) GL50803_ kda 100% 3 Proteasome subunit beta type 5 precursor location=glchr05: (+) GL50803_ kda 100% 3 Ribosomal protein S2 location=glchr05: ( ) length=242 GL50803_ kda 100% 100% 95% 100% 100% 97% Ribosomal protein L24A location=glchr05: (+) length=102 GL50803_ kda 100% 100% 100% Hypothetical protein location=glchr01: ( ) length=993 GL50803_ kda 100% 100% 100% Ribosomal protein S3a location=glchr05: ( ) length=248 GL50803_ kda 99% 100% 100% 100% 100% Ribosomal protein S4 location=glchr04: ( ) length=268 GL50803_ kda 99% 100% 100% 100% 100% Malic enzyme location=glchr05: ( ) length=557 GL50803_ kda 100% 100% 100% Histone H2A location=glchr03: ( ) length=124 GL50803_ kda 95% 95% 100% 100% 100% Hypothetical protein location=glchr04: ( ) length=886 GL50803_ kda 100% 100% 100% 100% Median body protein location=glchr05: (+) length=857 GL50803_ kda 100% 100% 3 2 AAA family ATPase location=glchr04: (+) length=870 GL50803_ kda 100% 9 Alcohol dehydrogenase location=glchr03: (+) length=888 GL50803_ kda 100% 7 Aspartyl trna synthetase location=glchr03: ( ) length=555 GL50803_ kda 100% 100% 2 5 Protein 21.1 location=glchr04: (+) length=786 GL50803_ kda 100% 100% 100% Ribosomal protein L12 location=glchr02: (+) length=182 GL50803_ kda 100% 4 Ribosomal protein S3 location=glchr02: (+) length=217 GL50803_ kda 100% 100% 100% Hypothetical protein location=glchr05: (+) length=985 GL50803_ kda 100% 3 Macrophage migration inhibitory factor location=glchr04: ( ) GL50803_ kda 100% 5 Ribosomal protein S11 location=glchr02: ( ) length=154 GL50803_ kda 99% 100% 96% Histone H3 location=glchr03: ( ) length=159 GL50803_ kda 100% 2 ADP ribosylation factor location=glchr05: (+) length=191 GL50803_ kda 96% 100% 1 3 ATP dependent RNA helicase location=glchr05: (+) length=900 GL50803_ kda 100% 6

14 Hypothetical protein location=glchr05: (+) length=403 GL50803_ kda 100% 5 Hypothetical protein location=glchr05: ( ) length=178 GL50803_ kda 100% 1 Ribosomal protein S21 location=glchr04: (+) length=89 GL50803_ kda 100% 1 Protein F17L21.10 location=glchr05: (+) length=122 GL50803_ kda 100% 2 Spastin location=glchr01: ( ) length=1118 GL50803_ kda 100% 100% 100% 100% 100% Ribosomal protein L10 location=glchr04: ( ) length=210 GL50803_ kda 100% 100% 99% 100% 100% 100% Pyruvate flavodoxin oxidoreductase location=glchr02: (+) GL50803_ kda 100% 100% 1 15 Hypothetical protein location=glchr04: (+) length=1216 GL50803_ kda 100% 100% 100% 100% 100% Ribosomal protein S23 location=glchr05: ( ) length=143 GL50803_ kda 100% 99% 100% 100% Ribosomal protein L23 location=glchr05: ( ) length=142 GL50803_ kda 100% 100% 100% 100% 100% 100% Histone H4 location=ch991767: ( ) length=99 GL50803_ kda 100% 100% 99% 100% Protein 21.6 location=glchr04: ( ) length=854 GL50803_ kda 95% 100% 100% 100% Ribosomal protein L21 location=glchr03: (+) length=159 GL50803_ kda 100% 100% 95% Acetyl CoA synthetase location=glchr03: ( ) length=726 GL50803_ kda 100% 11 Ribosomal protein L34 location=glchr04: ( ) length=120 GL50803_ kda 95% 100% 100% 100% Hypothetical protein location=glchr05: ( ) length=159 GL50803_ kda 100% 99% 9 1 Elongation factor 2 location=glchr04: (+) length=898 GL50803_ kda 100% 100% 2 7 Axoneme associated protein GASP 180 location=glchr05: ( ) GL50803_ kda 100% 11 Peptidyl prolyl cis trans isomerase B precursor location=glchr01: (+) GL50803_ kda 100% 100% 5 1 Nucleoside diphosphate kinase location=glchr04: (+) length=151 GL50803_ kda 100% 6 ATP dependent RNA helicase location=glchr04: ( ) length=625 GL50803_ kda 100% 100% 100% Ribosomal protein L18a location=glchr05: ( ) length=173 GL50803_ kda 100% 3 Wos2 protein location=glchr04: (+) length=185 GL50803_ kda 100% 95% 3 1 Nucleolar GTPase location=glchr04: (+) length=541 GL50803_ kda 100% 10 Acyl CoA synthetase location=glchr04: ( ) length=905 GL50803_ kda 100% 8 Hypothetical protein location=glchr04: ( ) length=281 GL50803_ kda 100% 96% 100% Adenine phosphoribosyltransferase location=glchr01: (+) GL50803_ kda 100% 4 Histone H3 location=glchr04: ( ) length=189 GL50803_ kda 100% 100% 100% 100% 100% 100% 100% 100% Ribosomal protein L3 location=glchr04: (+) length=379 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr05: ( ) length=367 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr02: (+) length=281 GL50803_ kda 100% 100% 100% 100% 100% 100% Hypothetical protein location=glchr05: (+) length=137 GL50803_ kda 100% 100% 100% 100% 100% 100% 100% Ribosomal protein L2 location=glchr05: (+) length=251 GL50803_ kda 100% 100% 1 5 BirA GL50803_ kda 100% 100% 100% 99% 100% 100% Giardia trophozoite antigen GTA 1 location=glchr03: ( ) GL50803_ kda 100% 100% 100% 100% 100% Protein 21.1 location=glchr05: (+) length=716 GL50803_ kda 100% 100% 100% Ribosomal protein S14 location=glchr04: (+) length=145 GL50803_ kda 100% 100% 100% Pyruvate, phosphate dikinase location=glchr04: ( ) length=884 GL50803_ kda 100% 12 Uracil phosphoribosyltransferase location=glchr05: ( ) length=211 GL50803_ kda 100% 100% 5 2 Ribosomal protein L27 location=glchr03: (+) length=135 GL50803_ kda 100% 95% 100% Hypothetical protein location=glchr05: (+) length=1330 GL50803_ kda 100% 12 Ribosomal protein S15A location=glchr04: ( ) length=130 GL50803_ kda 100% 100% 3 5 Acyl CoA synthetase location=glchr03: (+) length=970 GL50803_ kda 100% 9 Ribosomal protein S26 location=glchr04: ( ) length=109 GL50803_ kda 96% 100% 100% 100% Acidic ribosomal protein P0 location=glchr05: (+) length=326 GL50803_ kda 100% 98% 100% GTP binding nuclear protein RAN/TC4 location=glchr05: (+) GL50803_ kda 100% 100% 100% Ribosomal protein L37a location=glchr05: (+) length=94 GL50803_ kda 100% 100% 100% 100% 100% 100% 100% Hypothetical protein location=glchr01: (+) length=562 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr05: (+) length=826 GL50803_ kda 100% 100% 100% 100% Ribosomal protein S18 location=glchr01: ( ) length=154 GL50803_ kda 100% 100% 100% Cytosolic HSP70 location=glchr01: (+) length=664 GL50803_ kda 100% 92% 12 1 Carbamate kinase location=glchr02: (+) length=316 GL50803_ kda 100% 100% 100% Phosphoglycerate kinase location=glchr02: ( ) length=409 GL50803_ kda 100% 100% 11 1 Fructose bisphosphate aldolase location=glchr03: (+) length=323 GL50803_ kda 100% 10 Malate dehydrogenase location=glchr02: (+) length=331 GL50803_ kda 100% 100% 95% 2 4 1

15 Thioredoxin reductase location=glchr02: ( ) length=314 GL50803_ kda 99% 100% 100% Nucleolar GAR1 like protein, putative location=glchr02: (+) GL50803_ kda 100% 95% 4 1 Protein 21.1 location=glchr05: ( ) length=1024 GL50803_ kda 100% 100% 100% 100% 100% Ribosomal protein L4 location=glchr05: ( ) length=316 GL50803_ kda 100% 100% 100% 100% 100% 100% NADP specific glutamate dehydrogenase location=glchr04: ( ) GL50803_ kda 100% 100% 1 20 Ribosomal protein L23A location=glchr04: ( ) length=141 GL50803_ kda 100% 100% 100% 100% 100% 100% Hypothetical protein location=glchr05: (+) length=606 GL50803_ kda 100% 100% 100% 100% 100% Kinase, NEK location=glchr02: (+) length=515 GL50803_ kda 95% 100% 99% Protein 21.1 location=glchr05: (+) length=836 GL50803_ kda 100% 100% 100% 100% Kelch repeat containing protein location=glchr05: ( ) length=1514 GL50803_ kda 100% 7 Hypothetical protein location=glchr04: (+) length=1230 GL50803_ kda 100% 100% 100% 100% 100% Ribosomal protein S24 location=glchr04: (+) length=132 GL50803_ kda 100% 100% 100% 100% 100% Glyceraldehyde 3 phosphate dehydrogenase location=glchr05: ( ) GL50803_ kda 100% 100% 100% Peroxiredoxin 1 location=glchr03: ( ) length=201 GL50803_ kda 100% 100% 100% 100% 96% 100% 100% Ribosomal protein S5 location=ch991769: (+) length=190 GL50803_ kda 96% 99% 100% 100% Ribosomal protein S8 location=glchr03: ( ) length=174 GL50803_ kda 100% 100% 100% 100% 100% 100% Ribosomal protein S9 location=glchr01: ( ) length=189 GL50803_ kda 100% 100% 96% 100% Ribosomal protein S6 location=glchr01: (+) length=248 GL50803_ kda 100% 100% 100% 100% 100% 100% Ribosomal protein S13 location=glchr05: ( ) length=154 GL50803_ kda 100% 100% 100% 100% Ribosomal protein L17 location=glchr05: (+) length=164 GL50803_ kda 100% 100% 100% Ribosomal protein L18 location=glchr04: (+) length=179 GL50803_ kda 95% 100% 100% 100% 100% 100% Protein 21.1 location=glchr03: ( ) length=1146 GL50803_ kda 100% 100% 10 2 Enolase location=glchr05: (+) length=445 GL50803_ kda 100% 100% 100% Xaa Pro dipeptidase location=glchr01: (+) length=444 GL50803_ kda 100% 100% 95% Glycerol 3 phosphate dehydrogenase location=glchr05: ( ) GL50803_ kda 100% 17 Ribosomal protein L26 location=glchr04: (+) length=135 GL50803_ kda 100% 100% 100% 100% 100% 100% Spindle pole protein, putative location=glchr04: (+) length=780 GL50803_ kda 100% 100% 100% 100% 100% Ribosomal protein S17 location=glchr05: ( ) length=137 GL50803_ kda 100% 100% 9 6 UPL 1 location=glchr04: (+) length=310 GL50803_ kda 100% 100% 100% Ribosomal protein S16 location=glchr01: (+) length=158 GL50803_ kda 100% 95% 100% 100% 100% Ribosomal protein L19 location=glchr02: ( ) length=196 GL50803_ kda 100% 100% 100% 100% 100% 100% Ribosomal protein L13 location=glchr01: (+) length=234 GL50803_ kda 100% 100% 100% 100% 100% 100% 100% Alpha tubulin location=glchr03: ( ) length=454 GL50803_ kda 100% 100% 100% 100% 100% 100% 100% 100% Arginine deiminase location=glchr03: ( ) length=580 GL50803_ kda 100% 100% 100% Beta tubulin location=glchr05: ( ) length=447 GL50803_ kda 100% 100% 100% 100% 100% 100% 100% Kinase, NEK location=glchr02: (+) length=1117 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr05: (+) length=487 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr05: (+) length=988 GL50803_ kda 100% 100% 100% 100% 100% Elongation factor 1 alpha location=glchr02: (+) length=442 GL50803_ kda 100% 100% 100% 100% 100% 100% 100% Trichohyalin location=glchr04: ( ) length=1026 GL50803_ kda 100% 100% 100% 100% 100% Ribosomal protein L27a location=glchr05: (+) length=149 GL50803_ kda 100% 100% 100% 100% 96% 100% 100% 100% FtsJ cell division protein, putative location=glchr05: (+) GL50803_ kda 100% 100% 100% Ornithine carbamoyltransferase location=glchr03: (+) length=327 GL50803_ kda 100% 100% 100% 100% ATP/GTP binding protein, putative location=glchr04: (+) GL50803_ kda 100% 100% 100% 100% 100% 100% 100% 95% Coiled coil protein location=glchr02: (+) length=1374 GL50803_ kda 100% 100% 100% 100% Spindle pole protein, putative location=glchr01: ( ) length=995 GL50803_ kda 100% 100% 100% 100% 100% Hypothetical protein location=glchr01: ( ) length=385 GL50803_ kda 100% 100% 100% 100% 100% 100% GiOR1 G. intestinalis strain expressing cytosolic biotine ligase (cbira) and GiOR1 fused with biotin acceptor peptide Cia2 G. intestinalis strain expressing cytosolic biotine ligase (cbira) and Cia2 fused with biotin acceptor peptide cbira G. intestinalis strain expressing cbira (control) wt parent G. intestinalis strain (control)