22 (1) :70 76,2003 Journal of Mountain Agriculture and Biology 3 Ξ 1, 2, 1, 1 (11, 550025 ; 21, 330045) : [ Cordyceps sinensis (Berk. ) Sacc ],, 21, :;;; ; :S46711 :A :1008-0457 (2003) 01-0070 - 07 Research and appl ication prospect of polysaccharides of Cordyceps si nensis XIA O Jian2hui 1, JIA N G Nong2hui 2, L IA N G Zong2qi 1, L IU A i2ying 1 ( 1. Depart ment of Biotechnology, Biological and Environmental Science College, Guiz hou U niversity, Guiz hou Guiyang 550025, Chian ; 21 College of A gronomy, Jiangxi A gricultural U niversity, Jiangxi N anchang 330045, China) Abstract : In this paper,current situation on chemical structure and bioactivity of polysaccharides of Cordyceps sinensis (Berk. ) Sacc and its substitute were summarized. Combined with the achievements of studies on polysaccharides of the C. sinensis and its substitute and the edible and medicinal mushroom,the paper indicated that the connection of struc2 ture and function and the reciprocal actions of different components should be studied further. At the same time, the extensive bioactivity of Cordyceps, the profound effect of polysaccharides of edible and medicinal mushroom,good appli2 cation prospect of polysaccharides of C. sinensis and its substitutes were put forward. Key words : Cordyceps sinensis ; substitute ;edible and medicinal mushroom ;polysaccharide ;bioactivity E. Fischer,,,,,,,, 21,,, [1 ] 1993,Hart,,,,10,, [2 ],, 60 % 100 %300, 51 70, Ξ :2001-09 - 12 ;:2001-10 - 08 : (2001 1009) :(1972 - ),,,,
1, : 71 [3 ],,, C. si nensis,, C. si nensis,, [4 ] WTO,,, 1, C. si nensis,,,,,, C. si nensis,,, 1. 1,,,,,,,, 1. 2,,,, [5 ] 3, [6,7 ] 2 70,Miyazaki [8 ] C. si nensis, (CS - ) CS -, D - D - 1 1, Ki2 ho Na 2 CO 3 C. si nensis (CT - 4N),,,23uChen [10 ] C. si nensis PSCS(polysaccharide fraction of C. si nensis) U 937, - ( INF ) ( TNF) [ C. cicadae (Miquel) Massee ] (C - 3),, - D - (1-2) - D - (1-6), 4 3,27u [11,12 ] Kiho C. cicadae CI - P CI - A, 25u ;,39u
72 2003 CI - 5N 21u CI - 6P CI - 5N (1-6) (1-2) - D - (1-2) - D -,- D - 0-2 0-6 - D - - - D - ; CI - 6P (1-3) - - D -,(1-6) - - D -, [13,14 ] [ C. militaris (L. ) Link. ]CMPS,30u, 1 0. 5 51 99 2. 38 36. 5, 24. 17 % [15 ] C. sinensis ( 1) 1 C. sinensis Tab11 Research and exploitation of polysaccharides of Cordyceps sinensis and its substitute u Cordyceps sinensis C. cicadae Cephalosporium sinensis Cephalosporium sinensis C. sinensis CS - I CT - 4N PSCS C. cicadae C - 3 CI - 5N CI - 6P (123) 2D2 CI - P (123) 2D2 CI - A C. sinensis CS - F30 CS - F10 C. ophioglossoides CO - I (123) 2D2 CO - N SN - C C. sinensis C. militaris CM - 1 C. militaris C. militaris CMPS M ariannaea pruinosa Isaria sp. Metarhizium taii Paecilomyces hepiali Acremonium terricola Pae. gunnii var. minor Huang C. pruinosa PS1 PS2 23 27 39 21 25 25 45 15 632 50 700 270 C. sinensis C. taii CD - 1 200 C. sinensis CS81002 C. gunnii AT01 C. gi m mii PGM01 30 24 130 43,,, [ 8 10 ] [ 11 14 ] [ 16 18 ] [ 19 25 ] [26 ] [ 27 28 ] [ 15 ] [ 29 ] [ 30 ] [ 31 ] [ 32 ] [ 33 ] [ 33 34 ]
1, : 73 1,70 90, C. si nensis C. si nensis, C. si nensis,c. si nensis,, C. si nensis 3,,, ;,, C. si nensis,c. si nensis [16 18,28,33 ] [35 37 ],,,,,,,,,,,, 4, C. si nensis C. cicadae [ C. ophioglossoi des ( Ehrenb) Link ] [ Beauvera bassiana (Bals. ) Vullemin ],C. si nensis C. si nensis,,, C. si nensis, C. si nensis, C. si nensis [38 40 ], C. m ilitaris [ C. liangshanensis Zhang,Liu et Hu ] [ C. haw kesii ( Gray) Cooke ] [ C. gansuensis Zhang, Wang et Yan ] [ C. barnesis Thwaites ex Berk. Et Br. ] [ C. brasiliensis P. Henn ] [ C. takaomontana Samson ] [ C. scarabaeicola Kobayasi ] [39,41,42 ],,,, C. sinensis [ Paecilomyces hepiali Chen et Dai ] [ Cephalosporium sinensis Chen] [ Mortierella hepiali Chen et Lu] 5 C. si nensis,80, C. si nensis, 2,,, C. si nensis
74 2003 2 Tab12 Part edible and medicinal mushroom products in native market Hericium erinaceum Coriolus versicolor MAIEXT Grif ola f rondosa Grifron Grif ola. f rondosa Grif ola f rondosa Coriolus versicolor Coriolus versicolor Coriolus versicolor Coriolus versicolor Grif ola umbellata Grif ola umbellate Lenti m us edodes Lenti m us edodes Lenti m us edodes Lenti m us edodes Tremella f ucif ormis Tremella f ucif ormis Schizophyllum com m une Tramet us robiniophila Tramet us robiniophila Ganoderma lucidum Poria cocos Cordyceps sinensis Cordyceps militaris et al. 2 8 / kg / kg Paecilomyces hepiali Mortierella hepiali Hirsutella hepiali Cephlosporium sinensis Cephlosporium sinensis Cephlosporium sinensis Paecilomyces sinensis Cephlosporium sinensis Cordyceps militaris Hirsutella sinensis Cephlosporium sinensis Acremonium terricola Paecilomyces gunnii, C. si nensis,, C. si nensis, C. si nensis
1, : 75, C. si nensis,,,,,,,,,, [43 ] : [1 ] 1 :[J ]1,1999,19 (3) :103-106 1 [2 ] 1 [J ]1, 1999,7 (2) :145-1501 [3 ],1 [J ]1,1999,12 (2) :81-851 [4 ] 1 [A ]. 1 [ C]1 :,19991157-158. [5 ],,1 [J ]1,2000,35 (5) :397-4001 [6 ],,, 1 [J ]1,2000,15 (3) :107-1131 [7 ],1 [J ]1,1995,7 (3) :47-511 [ 8 ] Miyazaki T,Oikawa N, Yamada H1Studies on fungal polysaccharides XX galatomannan of Cordyceps sinensis[j ]1Chem Pharm Bull,1977,25 (12) :24-281 [9 ] Kiho T, Tabata H, Ukai S,et al. A minor,protein2containing galactomannan from a sodium carbonate extract of Cordyceps sinensis[j ]1Carbohyr Res,1986,156 :189-1971 [10 ] Chen Y J,Shiao M S,Lee S S, et al. Effect of Cordyceps sinensis on the proliferation and differentiation of human leukemic U 937 Cells[J ]1Life Sci,1997,60 (25) :2 349-2 3591 [11 ] Ukai S, Matsuura S, Hara C,et al. Structure of a new galactomannan from the ascocarps of Cordyceps cicadae Shing [J ] 1Carbohydr Res,1982, 101 :109-1161 [12 ] Ukai S, Kiho T,Hara C, et al. Poysaccharides in fungi X antitumor activity of various polysaccharides isolated from Doc2 tyophora indusiata, Ganoderm a japonicum, Cordyceps cicadae, A uricularia auricula2judae and A uricularia species [ J ] 1Chem Pharm Bull,1983,31 (2) :741-7441 [13 ] Kiho T,Ito M,Nagai K,et al. Polysaccharides in fungi XX a water2soluble polysaccharides from the alkaline extract of the insect2body portion of Chan hua ( Fungus : Cordyceps cicadae) [J ]1Chem Pharm Bull,1988,36 (8) :3 032-3 0371 [ 14 ] Kiho T,Ito M, Yoshida I,et al. Polysaccharides in fungi XX a (1-3) - D - glucan from the alkaline extract of the insect - body portion of Chan hua ( Fungus : Cordyceps cicadae) [J ]1Chem Pharm Bull,1989,37 (10) :2 7721 [15 ],,1 [J ]1,2000,17 (5) :361-3641 [16 ] Kiho T,Hui J, Yamane A,et al. Polysaccharides in fungi XXX hypoglycemic activity and chemical properties of a polysac2 charides from the cultural mycelium of Cordyceps sinensis[j ]1Biol Pharm Bull,1993,16 (12) :1 291-1 2931 [17 ] Kiho T, Yamane A,Ji H,et al. Polysaccharides in fungi XXX hypoglycemic activity of a polysaccharides(cs - F30) from the cultured mycelium of Cordyceps sinensis and its effect of glucose metabolism in mouse liver[j ]1Biol Pharm Bull,1996,19 (2) :294-296 1 [18 ] Kiho T,Ookubo K,Usui S,et al. Structural features and hypoglycemic activity of a polysaccharides(c S - F10) from the cul2 tured mycelium of Cordyceps sinensis[j ]1Biol Pharm Bull,1999,22 (9) :966-970 1 [ 19 ] Yamada H, Kawaguchi N,Ohmori T,et al. Structure and antitumor activity of an alkali2soluble polysaccharides from Cordy2 ceps ophioglossiodes[j ]1Carbohydr Res,1984,125 :107-1151 [20 ] Ohmori T, Tamura K, Wakaiki A, et al. Dissociation of a glucan fraction ( CO - 1) from protein2bound polysaccharides of Cordyceps ophioglossoides and analysis of its antitumor effect [J ]1Chem Pharm Bull,1988,36 (11) :4 512-4 5181 [ 21 ] Yamada H, Kawaguchi N,Ohmori T,et al. Structure of a galactosaminoglycan from Cordyceps ophioglossiodes[j ]1Carbohydr Res,1984,134 :275-2821 [22 ] Kawaguchi N,Ohmori T, Takeshita Y,et al. Occurrence of Gal (1-3) GalNAC - Ser/ Thr in the linkage region of poly2
76 2003 galactosamine containing fungal glycoprotein from Cordyceps ophioglossoides [J ] 1Biochem and Biophysi Res Comm, 1986, 140 (1) :350-3561 [ 23 ] Ohmori T, Tamura K,Fukui K, et al. Isolation of galactosaminoglycan moiety ( CO - N) from protein2bound polysaccharides of Cordyceps ophoglossoides and its effects against murine tumors[j ]1Chem Pharm Bull,1989,37 (4) :1 019-1 0221 [ 24 ] Ohmori T, Tamura K, Ohgane N, et al. The correlation between molecular weight and antitumor activity of galac2 tosaminoglycan (CO - N) from Cordyceps ophoglossoides[j ]1Chem Pharm Bull,1989,37 (5) :1 337-1 3401 [25 ] Ohmori T, Tamura K, Takaoka H, et al. Component analysis of protein2bound polysaccharides ( SN - C) from Cordyceps ophoglossoides and its effects on syngeneic murine tumors[j ]1Chem Pharm Bull,1988,36 (11) :4 505-4 5111 [26 ],, 1 [J ]1,1990, (1) :111 [ 27 ],1 I CM - 1d [J ]1,1992,11 (4) :300-3071 [28 ],1 Y 3 [J ]1,1998,28 (2) :192-197 1 [29 ],,, 1 [J ]1,1998,14 (3) :82-85 1 [30 ],,1 [J ]1,1996,19 (3) :315-3181 [31 ],,1 [J ]1,1993,6 (1) :27-321 [32 ],,, 1 [J ]1,1990,6 (6) :486-4911 [33 ],,1 [J ]1,2000,27 (4) :413-4161 [34 ],,, 1 [J ]1,2000,35 (1) :20-211 [35 ],,, 1 [J ]1,1997, (1) :1-41 [36 ],1 [J ]1,1998,29 (1) :94-951 [37 ] 1 [J ]1,2001,28 (3) :129-1321 [38 ],, 1 [J ]1,1998,21 (1) :80-851 [39 ] 1 [J ]1,2001,8 (2) :53-621 [40 ],,, 1 [J ]1,1999,30 (12) :950-9521 [41 ],1 [J ]1,1994,25 (5) :269-2711 [42 ],,1 [J ]1,1995,16 (1) :103-1081 [43 ],,, 1 [J ]1,1997,19 (3) :46-471