2005,38(10):20842089 Scientia Agricultura Sinica AFLP 1 1 1 2 1 ( 1 430070 2 430074) AFLP 20 19 918 324 35.29% SAS AFLP 0.9759 20 4 AFLP DNA AFLP Prunus mume Sieb. et Zucc. AFLP A Preliminary Analysis of Genetic Relationships for Prunus mume Sieb. et Zucc. by AFLP YANG Chaodong 1, WANG Jian 1, ZHANG Junwei 1, ZHANG Bo 2, BAO Manzhu 1 ( 1 College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070; 2 China Mei Flower Research Center, Wuhan 430074 ) Abstract: The genetic relationships among a variety, a hybrid and 18 Mei flower cultivars were preliminarily investigated using AFLP. Nineteen selected primer combinations revealed 918 legible loci, 324 of which were polymorphic ones that accounting for 35.29% of the total. Based on the SAS average similarity cluster analysis, 20 Mei samples were classified into four groups as =0.9759. P. mume var. cernua showed the highest polymorphism among these samples. Duoezhusha and Fubanxiaogongfen showed less polymorphism than P. mume var. cernua. In the group IV, AFLP and cluster analysis results were as follows: Parentages of some Mei flower cultivars were successfully identified. The results showed differences between Xiaogongfen, Jiangnanzhusha and their hybrids which would be useful to clear the confusions of homonyms and synonyms. There were close genetic relationships among cultivars of pendulous group, which supported traditionally classified system using branch gesture as the important morphological character in Mei flowers. The pendulous cultivars were more evolutionary than others, which is in conform with their cultivated history. These results demonstrate that AFLP technique is an efficient method for evaluating the genetic relationships and diversity of Mei flower. Key words: Prunus mume; AFLP; Cluster analysis; Genetic relationships Prunus mume Sieb. et Zucc. 3 000 5 000 [1,2] 20041209 30170666 1971 Tel: 02787282435 Fax: 02787282095 Email: mzbao@mail.hzau.edu.cn
10 AFLP 1 [1,3] [3] 1 [1,4] 1.1 20 1 [3 5] RAPD 1.2 [6] 1.2.1 DNA 2003 4 [7 8] DNA SDS [9] [9] 1.2.2 AFLP [16] 3 Vos [17] EcoR I Pst I 5 18 [10] DNA 2 [11] [12] 1.2.3 AFLP : 67 500 bp [13] AFLP, 1 0 19 [14] [15] DNA SAS AFLP 2 AFLP DNA 2.1 AFLP DNA DNA AFLP [18] 19 1 Table 1 Experiment materials No. Line Parents No. Line Parents 1 Danhongchuizhi 11 Dayu 2 Moshanchuizhi 12 Fenpigongfen 3 Qugengmei 13 Xuemei Fenpigongfen 4 Xuemei 14 Duoezhusha 5 Xiaohongchangxu 6 Danlunzhusha 15 Fubanxiaogongfen 16 Tiaoxuechuizhi 7 Xiaogongfen 17 Canxue 8 Jiangnanzhusha 18 Fenpichuizhi 9 Jiangshagongfen 10 Jiangnantaige 19 Shuangbichuizhi 20 Jingshengchuizhi
2086 38 EGT/PAG 20 AFLP 35.29% 48.31 17.05 6% PAGE 2 1 2.2 19 SAS AFLP 918 324 2 λ 0.9759 18 4 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 M 489 bp 404 bp 331 bp 2.3 242 bp 2 190 bp 147 bp 110 bp AFLP : [3] 67 bp 1 20 20 M puc mix8 markers 120 were the samples in the studies, M were the puc mix8 markers 1 EGT/PAG 20 AFLP AFLP Fig. 1 AFLP profiles of 20 Mei samples generated by the primer combination of EAT/PAT
10 AFLP 2087 2 20 AFLP Table 2 The amplification results of AFLP primer combinations in 20 Mei samples Primer combination No. of amplified loci No. of polymorphic loci Ratio of polymorphic loci (%) EAT/PAT 37 23 62.16 ETG/PAG 55 20 36.36 ETG/PTT 44 18 40.90 ETC/PAA 58 27 46.55 ETA/PAT 39 15 38.46 EGC/PCT 53 18 33.96 EAC/PTA 75 28 37.33 EAG/PGC 46 18 39.13 EGT/PAG 53 20 37.73 EGT/PTA 56 27 48.21 EGC/PAC 57 13 22.80 ETT6/PAC 51 13 25.49 ECA/PAG 46 13 28.26 EAC/PAA 30 13 43.33 EGA/PCA 34 11 32.35 EGC/PTC 46 12 26.08 ECA/PCC 36 13 36.11 ETG/PAC 40 11 27.50 EGG/PAT 62 11 17.74 Total 918 324 35.29 P. Pst ; E. EcoR 2 20 AFLP SAS Fig. 2 Dendrogram of cluster analysis of 20 Mei samples based on AFLP data
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