Hydrogen sulfide promotes the osteogenesis of bone marrow mesenchymal cells through hypoxia-inducible factor-1 alpha under tensile stress

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21 21 2017 07 28 Chinese Journal of Tissue Engineering Research July 28, 2017 Vol.21, No.21 1α ( 423000). 1α [J]. 2017 21(21):3281-3286. DOI:10.3969/j.issn.2095-4344.2017.21.001 ORCID: 0000-0002-2701-9583() 1α 1981 2010 423000 (5 7 d)(1.0 1.5 mm/d) 1 1 1 1 :R318 :A :2095-4344 (2017)21-03281-06 2017-04-26 1α 2 000 µstrain 1α 1α 1α GYY4137 YC-1 1 α (81301651)(N2015-006) Hydrogen sulfide promotes the osteogenesis of bone marrow mesenchymal cells through hypoxia-inducible factor-1 alpha under tensile stress Jiang Xiao-wen, Zhang Yi, Fan Xiao-sheng, Chen Yan-ze (Department of Stomatology, the First People s Hospital of Chenzhou & Institute of Translation Medicine, University of China South, Chenzhou 423000, Hunan Province, China) Abstract BACKGROUND: Hydrogen sulfide signaling has been proved to promote distraction osteogenesis; however, the underlying mechanism remains unclear. OBJECTIVE: To evaluate the relationship between hydrogen sulfide and hypoxia-inducible factor-1α (HIF-1α) Jiang Xiao-wen, Associate chief physician, Department of Stomatology, the First People s Hospital of Chenzhou & Institute of Translation Medicine, University of China South, Chenzhou 423000, Hunan Province, China Corresponding author: Jiang Xiao-wen, Department of Stomatology, the First People s Hospital of Chenzhou & Institute of Translation Medicine, University of China South, Chenzhou 423000, Hunan Province, China ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 3281

. 1α during the osteogenesis of rat bone marrow mesenchymal cells under tensile stress. METHODS: 2 000 µ strain was loaded on the in vitro cultured rat bone marrow mesenchymal cells by a four-point bending apparatus, and hydrogen sulfide donor or HIF-1α inhibitor was adopted in the tensile unit. Subsequently, the levels of osteogenic markers were detected. RESULTS AND CONCLUSION: Exogenous hydrogen sulfide signaling could promote the osteogenesis of rat bone marrow mesenchymal cells under tensile stress. However, this promotion was obviously eliminated when the endogenous HIF-1α expression was inhibited. These results show that hydrogen sulfide signaling system promotes the osteogenesis of rat bone marrow mesenchymal cells under tensile stress probably through HIF-1α. Subject headings: Tissue Engineering; Hydrogen Sulfide; Hypoxia-Inducible Factor 1, alpha Subunit Funding: the National Natural Science Foundation of China, No. 81301651; the Key Project of the First People s Hospital of Chenzhou, No. N2015-006 Cite this article: Jiang XW, Zhang Y, Fan XS, Chen YZ. Hydrogen sulfide promotes the osteogenesis of bone marrow mesenchymal cells through hypoxia-inducible factor-1 alpha under tensile stress. Zhongguo Zuzhi Gongcheng Yanjiu. 2017;21(21):3281-3286. 0 Introduction (distraction osteogenesis DO) [1] [1] [2-10] [11] (H 2 S) L [12] [13-14] [15] H 2 S3 H 2 S β 3 L H 2 S [16] H 2 S H 2 S H 2 S [17-19] (hypoxia-inducible factors HIFs) [20] H 2 S [21-22] H 2 S (bone marrow mesenchymal cell BMMC) 1α 1α H 2 S 1Materials and methods 1.1 1.2 2015 9 2016 2 1.3 2 SD250 350 g SYXK( ) 2010-0006 1.4 1.4.1 Percoll( Gibco ) 5% PBS 3 10% LG-DMEM( Gibco ) 2 d 70% 80% 0.25% 3 1.4.2 1.5 10 5 /cm 2 [1] 24 h 3282 P.O. Box 10002, Shenyang 110180

. 1α 60 min( ( ) 0.5 Hz 1 mm 2 000 µ strain0.2% 4 8 1 2 1α 1 α (YC-1) 80 µmol/l 3 H 2 S GYY41374 mmol/l 4 1α /H 2 S 3 YC-180 µmol/l 2 h 1.4.3 24 h PBS 1.4.4 H 2 S (10 ml 1 cm 0.8 cm ) 1 mol/l HCl 0.5 mlh 2 S 1 mol/l NaOH 0.5 ml H 2 S37 4 h H 2 S [17] 1.4.5 2 h PBS 3 ml 2 ml PBS 90 µl PBS 3 ( )450 nm U/mg 1.4.6 450 nm 2 h 1 ml 10 µl 40 µl 37 30 min 50 µl 37 30 min 37 15 min 450 nm 1.4.7 (PINP) ( ) 450 nm 2 h 1 ml 5 µl 45 µl PINP 30 min 150 µl 30 min 30 min 450 nm PINP 1.4.8 Real time-pcr 2 h Trizol(Gibco) RNA; (TaKaRa) BioRad 1 000 TM cdna 1 cdna SYBR Green RT-PCR GAPDH PCR 5 -CTT CGT CAG CGT CCT ATC AGT TC-3 5 -CAG CGT CAA CAC CAT CAT TCT G-3 2 (runt-related transcription factor 2 Runx2) mrna 5 -TAT GAC TCT ACC CAC GGC AAG T-3 5 -ATA CTC AGC ACC AGC ATC ACC-3 PCR95 30 s 95 5 s 57 30 s 40 Runx2 GAPDH 1.4.9 Western Blot 1α BCA ( Gibco ) 20 µg SDS-PAGE PVDF (1α1 500 Abcam ) (lgg 1 500 )37 1 h 1.5 H 2 S (PINP) Runx2 1α 1.6 SPSS 13.0 x _ ±s LSD P < 0.05 2Results 2.1 4 2 h 4 (P > 0.05 1) 2.2 H 2 S 4 2 h H 2 S 1α/ H 2 SH 2 S 2(P < 0.05 1) 2.3 H 2 S 1α/H 2 S (P < 0.05 1) H 2 S 2.4 H 2 S 3 (P < 0.05 1) 2.5 PINP 1α /H 2 S (P < 0.05 1) H 2 S PINP 2.6 Runx2 mrna H 2 S Runx2 mrna 4 (P < 0.05 1) ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 3283

. 1α 1 H 2S Table 1 Levels of osteogenic markers and hydrogen sulfide at the end of tensile stress loading (x _ ±s n=8) 1α H 2S 1α / H 2S (10 6 ) 6.22±0.80 5.67±0.75 6.21±0.66 6.38±0.61 H 2S(U/mg) 17.84±1.48 15.70±3.95 35.45±3.29 a 35.43±3.44 a (U/mg) 194.12±15.40 183.69±22.98 241.35±26.81 a 209.06±27.18 a (U) 1.48±0.16 1.17±0.17 1.91±0.25 a 1.28±0.18 PINP(µg/L) 8.00±0.26 2.23±0.35 4.81±0.57 a 3.16±0.37 Runx2 mrna 10.97±1.29 9.23±1.21 15.06±2.65 a 11.13±0.81 1α a P < 0.05 PINP A B C D M r 120 000 1α β-actin 43 000 1 1α Figure 1 Protein expression of hypoxia-inducible factor-1α in rat bone marrow mesenchymal cells at the end of tensile stress loading A D 1α H 2S 1α /H 2S B D 1α 2.7 1α Western blot4 hh 2 S 1α H 2 S 1α 1α1α /H 2 S ( 1) 3Discussion H 2 S 3 H 2 S [23-25] H 2 S H 2 S [17-18] GYY4137 H 2 S H 2 S [19] [26] [20] H 2 S H 2 S H 2 S Runx2 PINP Runx2 PINPH 2 S H 2 S H 2 S H 2 S H 2 S [27-28] α 3 α (1α 2α 3α) 1α H 2 S [29] Liu [21] H 2 S Bir [22] H 2 S [30] H 2 S [31] H 2 S 1α 1α mrna 1α [32-51] Wan [52] 3284 P.O. Box 10002, Shenyang 110180

. 1α H 2 S 1α H 2 S H 2 S H 2 S H 2 S H 2 S 2009 Ethical issues in animal experimentation CNKI 3 ( ) 3.0 4 References [1].[J]., 2005,40(1):10-12. [2] Jiang XW, Zou SJ, Ye B, et al. bfgf-modified BMMSCs enhance bone regeneration following distraction osteogenesis in rabbits. Bone. 2010;46(4):1156-1161. [3],,,.Runt 2 [J].,2016, 34(2):125-129. [4] Kitoh H, Kitakoji T, Tsuchiya H, et al. Transplantation of marrow-derived mesenchymal stem cells and platelet-rich plasma during distraction osteogenesis-a preliminary result of three cases. Bone. 2004;35(4):892-898. [5] Kitoh H, Kitakoji T, Tsuchiya H, et al. Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long bones. Bone. 2007;40(2): 522-528. [6] Schiller JR, Moore DC, Ehrlich MG. Increased lengthening rate decreases expression of fibroblast growth factor 2, platelet-derived growth factor, vascular endothelial growth factor, and CD31 in a rat model of distraction osteogenesis. J Pediatr Orthop. 2007;27(8):961-968. [7] Li G, Bouxsein ML, Luppen C, et al. Bone consolidation is enhanced by rhbmp-2 in a rabbit model of distraction osteogenesis. J Orthop Res. 2002;20(4):779-788. [8],,. [J].,2016,32(2):249-252. [9],,,. -2 -β1 [J]., 2016,33(5):679-683. [10] Chan CW, Qin L, Lee KM, et al. Low intensity pulsed ultrasound accelerated bone remodeling during consolidation stage of distraction osteogenesis. J Orthop Res. 2006;24(2): 263-270. [11],. [J]., 2016,37(4):242-246. [12],./ [J].,2007,28(4):603-606. [13],,. [J].,2005,5(1):61-65. [14],,,. [J].,2009,27(6):676-680. [15],,,. [J].( ),2016,17(1):62-65. [16],. [J].,2009,16(2):170-173. [17],,,. [J].,2015, 23(1):21-24. [18],,,. [J]., 2015,31(20): 3291-3294. [19] Jiang XW, Chen YZ, Lu K, et al. GYY4137 promotes bone formation in a rabbit distraction osteogenesis model: a preliminary report. J Oral Maxillofac Surg. 2015;73(4): 732.e1-e6. [20] Jiang XW, Zhang Y, Fan XS, et al. The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: ananimal study. Int J Oral Maxillofac Surg. 2016;45(2):267-272. [21] Liu X, Pan L, Zhuo Y, et al. Hypoxia-Inducible Factor-1α is involved in the pro-angiogenic effect of hydrogen sulfide under hypoxic stress. Biol Pharm Bull. 2010;33(9):1550-1554. [22] Bir SC, Kolluru GK, McCarthy P, et al. Hydrogen sulfide stimulates ischemic vascular remodeling through nitric oxide synthase and nitrite reduction activity regulating hypoxiainducible factor-1α and vascular endothelial growth factordependent angiogenesis. J Am Heart Assoc. 2012;1(5): e004093. ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 3285

. 1α [23],,,.PI3K/Akt /Sirt1 [J].,2016,32(2): 268-273. [24],,,. / [J].,2010, 26(7):944-947. [25],,,. / [J].,2008,24(7):910-914. [26] He JF, Xie ZJ, Zhao H, et al. Immunohistochemical and in-situ hybridization study of hypoxia inducible factor-1alpha and angiopoietin-1 in a rabbit model of mandibular distraction osteogenesis. Int J Oral Maxillofac Surg. 2008;37(6):554-560. [27],,,.1 2: [J].,2011,23(6):753-761. [28],,,.2α [J].,2016,20(7):917-924. [29] Budde MW, Roth MB. Hydrogen sulfide increases hypoxia-inducible factor-1 activity independently of von Hippel-Lindau tumor suppressor-1 in C. elegans. Mol Biol Cell. 2010;21(1):212-217. [30],,,. α[j].,2011,20(7):687-691. [31],,,.1 [J]., 2012,64(2):129-134. [32] Edelmayer M, Al-Habbal D, Pensch M, et al. Effect of prolyl hydroxylase inhibitor-loaded collagen barrier membranes on osteoclastogenesis and osteoblastogenesis. J Biomater Appl. 2017. doi: 10.1177/0885328217702563. [33] Böhm AM, Dirckx N, Maes C. Recruitment of osteogenic cells to bone formation sites during development and fracture repair - German Version. Z Rheumatol. 2016;75(3):316-321. [34] Stegen S, van Gastel N, Eelen G, et al. HIF-1α Promotes Glutamine-Mediated Redox Homeostasis and Glycogen-Dependent Bioenergetics to Support Postimplantation Bone Cell Survival. Cell Metab. 2016; 23(2):265-279. [35] Zhang T, Xie J, Sun K, et al. Physiological oxygen tension modulates soluble growth factor profile after crosstalk between chondrocytes and osteoblasts. Cell Prolif. 2016; 49(1):122-133. [36] Kuroyanagi G, Otsuka T, Yamamoto N, et al. Mimosine suppresses the PGF2α-induced synthesis of osteoprotegerin but not interleukin-6 in osteoblasts. Int J Mol Med. 2016;37(2): 533-541. [37] Cui M, Kanemoto S, Cui X, et al. OASIS modulates hypoxia pathway activity to regulate bone angiogenesis. Sci Rep. 2015;5:16455. [38] Huang J, Liu L, Feng M, et al. Effect of CoCl 2 on fracture repair in a rat model of bone fracture. Mol Med Rep. 2015; 12(4):5951-5956. [39] Chen NX, O'Neill KD, Allen MR, et al. Low Bone Turnover in Chronic Kidney Disease Is Associated with Decreased VEGF-A Expression and Osteoblast Differentiation. Am J Nephrol. 2015;41(6):464-473. [40] Xing Y, Wang R, Chen D, et al. COX2 is involved in hypoxia-induced TNF-α expression in osteoblast. Sci Rep. 2015;5:10020. [41] Quinlan E, Partap S, Azevedo MM, et al. Hypoxia-mimicking bioactive glass/collagen glycosaminoglycan composite scaffolds to enhance angiogenesis and bone repair. Biomaterials. 2015;52:358-366. [42] Johnson RW, Schipani E, Giaccia AJ. HIF targets in bone remodeling and metastatic disease.pharmacol Ther. 2015; 150:169-177. [43] Liu XD, Cai F, Liu L, et al. MicroRNA-210 is involved in the regulation of postmenopausal osteoporosis through promotion of VEGF expression and osteoblast differentiation. Biol Chem. 2015;396(4):339-347. [44] Peng J, Lai ZG, Fang ZL, et al. Dimethyloxalylglycine prevents bone loss in ovariectomized C57BL/6J mice through enhanced angiogenesis and osteogenesis. PLoS One. 2014; 9(11):e112744. [45] Kawao N, Tamura Y, Okumoto K, et al. Tissue-type plasminogen activator deficiency delays bone repair: roles of osteoblastic proliferation and vascular endothelial growth factor. Am J Physiol Endocrinol Metab. 2014;307(3): E278-E288. [46] Regan JN, Lim J, Shi Y, et al. Up-regulation of glycolytic metabolism is required for HIF1α-driven bone formation. Proc Natl Acad Sci U S A. 2014;111(23):8673-8678. [47] Kusumbe AP, Ramasamy SK, Adams RH. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature. 2014;507(7492):323-328. [48] Zhao G, Liu Y, Fang J, et al. Dimethyl fumarate inhibits the expression and function of hypoxia-inducible factor-1α (HIF-1α). Biochem Biophys Res Commun. 2014;448(3): 303-307. [49] Liu X, Tu Y, Zhang L, et al. Prolyl hydroxylase inhibitors protect from the bone loss in ovariectomy rats by increasing bone vascularity. Cell Biochem Biophys. 2014;69(1):141-149. [50] Dirckx N, Van Hul M, Maes C. Osteoblast recruitment to sites of bone formation in skeletal development, homeostasis, and regeneration. Birth Defects Res C Embryo Today. 2013;99(3): 170-191. [51] Dorjgochoo T, Zheng Y, Gao YT, et al. No association between genetic variants in angiogenesis and inflammation pathway genes and breast cancer survival among Chinese women.cancer Epidemiol. 2013;37(5):619-624. [52] Wan C, Gilbert SR, Wang Y, et al. Activation of the hypoxia-inducible factor-1α pathway accelerates bone regeneration. Proc Natl Acad Sci USA. 2008;105(2):686-691. 3286 P.O. Box 10002, Shenyang 110180