Effects of Polygonatum sibiricum Polysaccharide on Immunosuppressive Action Induced by Cyclophosphamide in Mice

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1 37 2 Vol37 No Journal of South-Central University for NationalitiesNatSciEdition Jun MTT 200 μg ml -1 ConA 700% ConA IL-6 TNF-α 150 mg kg %ConA 433% 1483% ConA 485% Q956 A Effects of Polygonatum sibiricum Polysaccharide on Immunosuppressive Action Induced by Cyclophosphamide in Mice Deng Xukun 1 Duan Huan 1 Liu Zhao 2 Shu Guangwen 1 Jiang Shanqing 1 Yu Huifan 1 1 College of Pharmaceutical SciencesSouth-Central University for NationalitiesWuhan China 2 Editorial Department of University Journal South-Central University for Nationalities Wuhan China Abstract ObjectiveTo investigate the effects of Polygonatum sibiricum polysaccharidepspon the immunosuppression activities induced by cyclophosphamide in mice MethodsMTT assay was used to evaluate the proliferation activity of splenocytes of normal mice Mice immunosuppression model was established with cyclophosphamide The mice spleen thymus index and spleen cellthymocyte proliferation activity in vitromacrophage phagocytosisand the ability of macrophages to secrete inflammatory cytokines were respectively determined ResultPSP could evidently increase the proliferation activity of spleen cell of normal mice The synergistic proliferation promotion rate with ConA was more than 700% with PSP dose of 200 μg ml -1 PSP could also improve the spleen and thymus index of immunosuppressed mice promote the proliferation of spleen and thymus cells stimulated by ConAand enhance the phagocytosis of macrophage and the secretion of IL-6 and TNF-α Compared with the control group the spleen index increased by 790%433% in high PSP dose 150 mg kg -1 for ConA synergized groupand the thymus indexes increased by 1483%485% respectively ConclusionPSP can significantly alleviate the immunosuppression of mice induced by cyclophosphamide Keywords Polygonatum sibiricum polysaccharidecyclophosphamideimmunosuppression Polygonatum 1 kingianum Coll et Hemsl Polygonatum sibiricum Red Polygonatum cyrtonema Hua dxk711208@ 163com BAI27B06

2 mg kg -1 IL ml g -1 2 mg 3 d 4 d ml RPMI min 7 ~ 9 SPF 18 ~ 22 g 2 RPMI-1640 SCXK d 95% 12 6 Polygonatum = mg/ g sibiricum Red RPMI-1640 RPMI-1640 Genom Tissue Culture Biologicals MTT ConA LPSSigma IL- 1β IL-6 TNF-α Spectra Max M3 Dynex CO 2 BB15 Thermo BL h 3 Suprafuge22 Heraeus 200 μl RE52CS-1 24 / % NH 4 Cl ph /37 5% CO 2 4 h PBS 100 μl 0 04% 12 h OD ConA g 95% /24 h 60 3 h 80% 60 2 h 100 μl ConA2 μg ml RPMI h 10 μl / MTT 4 h 150 μl 3 h 80% DMSO OD 570 ConA 4 = OD / 48 h OD 7-27 IL-1βIL-6TNF-α 969% /

3 μg ml -1 LPS24 h t x± 珋 s P<005 ELISA IL-1βIL-6 TNF-α / 6 ConA ConA μg /ml ConA μg ml -1 ConA 10 μl OD 570 OD 150 mg kg SPSS 190 P< % ConA 309% P < 005 μg ml h 10 μl MTT 4 h 150 μl DMSO ConA 790% P <001ConA 433%P<005 Tab1 1 ConA x±sn 珋 = 10 Effects of PSP on spleen index and proliferation ofsplenocyte stimulated by ConA in immunosuppression mice /mg kg -1 / mg g -1 ConA 399± ± ± ± ± ± ±029 * 582± ±026 ** 639±081 * P<005 * ** P< ConA ConA 150 mg kg %P<001ConA ConA 485%P< %345%P<005 Tab2 2 ConA x±sn 珋 = 10 Effects of PSP on thymus index and proliferation ofthymocyte stimulated by ConA in immunosuppression mice /mg kg -1 / mg g -1 ConA 299± ± ± ± ± ± ±027 * 528± ±039 ** 646±083 * P<005 * ** P<001

4 βIL-6 TNF-α 33 IL- TNF-α P <005 IL- 1β IL-6TNF-α 3 3 IL-6 P<001 Tab3 3 IL-6 TNF-α 150 mg kg -1 IL-6 TNF-α IL-1βIL-6TNF-α x±sn 珋 = 10 Effects of PSP on the secretion of IL-1βIL-6TNF-α in macrophages of immunosuppression mice /mg kg -1 / pg ml -1 IL-1β IL-6 TNF-α 3374± ± ± ± ± ± ± ± ± ± ±475 * 17863±642 * ±353 * 898±501 ** 18018±343 ** P<005 P<001 * P<005 ** P< P<001 P<005 1 P<001 * P<005 Fig1 Effect of PSP on phagocytosis of macrophages in immunosuppression mice ConA ConA ConA μg ml -1 9 P < ConA 760% Tab4 珋 x±sn =6 Effect of PSP on the proliferation activity of spleen cells in normal mice / μg ml -1 ConA ± ± ± ± ± ±037 * ±030 * 414±043 ** ±057 ** 739±076 *** ±087 *** 760±105 *** * P<005 ** P<001 *** P< T B 8

5 2 53 TNF-αIL-1β IL-2 IL-6 J LPS 5 J IL-6 TNFα J ConA J 2016 IL-6 TNF-α J J M 10Schepetkin I AQuinn M T Botanical polysaccharides macrophage immunomodulation and therapeutic potential 2 J J Int Immunopharmacol Liu J Qi YZheng Let al Xinfeng capsule improves 3 pulmonary function in ankylosing spondylitis patients via J NF-κB-iNOS-NO signaling pathway J J Tradit Chin Med 櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖櫖 19 LiNi 1/3 Co 1/3 Mn 1/3 O 2 powders cathode material for Li-ion batteries J J Power Sources Florian S Prasant K N Evan M E et al Study of cathode materials for lithium-ion batteriesrecent progress and new challenges J Inorganics Yuan X Xu Q J Liu X et al Excellent rate performance and high capacity of Mo doped layered cathode material Li Li 02 Mn 054 Ni 013 Co 013 O 2 derived from an improved coprecipitation approach J Electrochim Acta CeO 2 J Rosedhi N D Idris N H Rahman M M et al Disordered spinel LiNi 05 Mn 15 O 4 cathode with improved rate performance for lithium-ion batteries J Electrochim Acta Han X Meng Q Sun T et al Preparation and electrochemical characterization of single-crystalline spherical 6Zhang S Deng C Fu B L et al Synthetic optimization of spherical LiNi 1/3 Mn 1/3 Co 1/3 O 2 prepared by a carbonate co-precipitation method J Powder Technol Wang T Liu Z H Fan L et al Synthesis optimization of Li 1+x Mn 045 Co 040 Ni 015 O 2 with different spherical sizes via co-precipitation J Powder Technol Zheng Z Guo X D Chou S L et al Uniform Ni-rich LiNi 06 Co 02 Mn 02 O 2 porous microspheresfacile designed synthesis and their improved electrochemical performance J Electrochim Acta Ellis B Subramanya Herle P Rho Y H et al Nanostructured materials for lithium-ion batteriessurface conductivity vs bulk ion/electron transport J Faraday Discuss

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