(mycelial polysaccharides from Cordyceps sinensis MPCS) sinensis) (BCG+LPS) ALT AST (P<0.01) [DOI] /j.issn

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1 [ ] (BCG+LPS) 6 NIH (1 5 25mg/kg) (15mg/kg) 1 ALT AST NO IL-1 TNF- Real-time PCR IL-6 inos mrna ALT AST (P<.1) NO IL-1 TNF- (P<.1) IL-6 inos mrna (P<.1) [ ] [ ] R [ ] A [ ] (216) [DOI] /j.issn Protective effect of mycelial polysaccharides from Cordyceps sinensis on immunological liver injury in mice DONG Kai-zhong 1, GAO Yong-sheng 2, WANG Xiao-heng 1, MA Yan-qing 1, SU Lu 1 1 Medical College, Northwest University for Nationalities, Lanzhou 733, China 2 Tianzhu Tibetan Autonomous County People s Hospital, Wuwei, Gansu 7332, China This work was supported by the Fundamental Research Funds for the Central Universities (ZYZ21292) [Abstract] Objective To explore the protective effects of mycelial polysaccharides from Cordyceps sinensis (MPCS) on BCG+LPS-induced liver injury in mice. Methods The immunological liver injury mice model was reproduced by giving bacillus Calmette-Guerin (BCG) and lipopolysaccharide (LPS). Sixty NIH mice were randomly assigned into 6 groups (1 each): normal control group, model group, mycelium polysaccharide in high (1mg/kg), medium (5mg/kg) and low (25mg/kg) dose group, and bifendate (15mg/kg) treatment group. The serum transaminase levels of alanine ALT and AST were assayed with ELISA, nitric oxide (NO) in serum was measured by nitrate reductase method, and the liver homogenate was prepared for the determination of the contents of interleukin-1 (IL-1 ) and tumor necrosis factor- (TNF- ). The mrna expression levels of IL-6 and inos in hepatic tissue were assessed using RT-PCR. Results In the mice of immunological liver injury, mycelial polysaccharides from Cordyceps sinensis obviously lowered the serum ALT and AST levels (P<.1), high dose MPCS significantly reduced the serum NO and liver tissue IL-1 and TNF- levels (P<.1). Compared with the model group, high and medium dose MPCS significantly reduced the expression levels of IL-6 and inos mrna in hepatic tissues (P<.1). Conclusion MPCS shows a certain protective effect on immunological liver injury induced by BCG plus LPS in mice. [Key words] mycelium; polysaccharides; liver injury [1] (Cordyceps sinensis) [ ] (ZYZ21292) [ ] [ ] 733 ( ) 7332 ( ) [2] [3] (mycelial polysaccharides from Cordyceps sinensis MPCS) (BCG+LPS)

2 Med J Chin PLA, Vol. 41, No. 4, April 1, SPF NIH 2±2g SCXK( )211-4 (Sigma ) ( ) ALT AST NO ( ) IL-1 TNF- (RD ) ( ) 1.2 (BCG) (LPS) [4-5] BCG (.2ml/ ) 12d LPS(.2ml/ 7.5 g) 1.3 NIH (1mg/kg) (5mg/kg) (25mg/ kg) (15mg/kg) 1d 1 /d(.5% ) LPS( ) 16h 12h 1.4 1g [6] 1 12min 1: % 4 24h 8r/min 1min 2 5 Sevag [7] DEAE-cellulose 1.5 ALT AST ALT AST ALT AST (U/L) 1.6 NO ( ) [8] 1min 55nm (A) NO 1.7 IL-1 TNF- 1% ELISA IL-1 TNF- 1.8 Real-time PCR IL-6 inos mrna Primer Premier 5. GenBank mrna IL-6 5'-CCACTTCACAAGTCGGAG- GCTTA-3' 5'-GCAAGTGCATCATCGTTGTTC- ATAC-3' inos 5'-GGCAGCCTGTGAGACCTT- TG-3' 5'-GCATTGGAAGTGAAGCGTTTC-3' -actin 5'-CCACAGCTGAGAGGGAAATC-3' 5'-TCTCCAGGGAGGAAGAGGAT-3' Total RNA RNA 37 1h 95 3min Real-time PCR 93 3min 93 3s 55 45s 72 45s 38 -actin IL-6 inos mrna 1.9 SPSS 13. x±s Student-t χ 2 P< ALT AST (P<.1) ALT AST (P<.1 P<.5 1) 2.2 NO NO Transaminase concentration (U/L) Median dose MP group ALT AST 1 BCG+LPS Fig.1 Effects of mycelial polysaccharides from Cordyceps sinensis on serum ALT and AST levels of mice with immunological liver injury induced by BCG plus LPS P<.1 compared with control group; P<.1, P<.5

3 (P<.1) NO (P<.1 P<.5 2) NO content ( mol/l) Median dose MP group 2 BCG+LPS NO Fig.2 Effects of mycelial polysaccharides from Cordyceps sinensis on serum NO levels of mice with immunological liver injury induced by BCG plus LPS P<.1 compared with control group; P<.1, P< IL-1 TNF- IL-1 TNF- (P<.1) IL-1 TNF- (P<.1 P<.5 3) Median dose MP group IL-1 TNF- 3 BCG+LPS IL-1 TNF- Fig.3 Effects of mycelial polysaccharides from Cordyceps sinensis on IL-1 and TNF- levels in liver homogenate of mice with immunological liver injury induced by BCG plus LPS P<.1 compared with control group; P<.1, P<.5 Content (pg/ml) 2.4 IL-6 inos mrna PCR IL-6 inos mrna (P<.1) IL-6 inos mrna (P<.1 P<.5 4) IL-6 inos mrna mrna expression MediandoseMPgroup IL-6 4 IL-6 inos mrna Fig.4 Effects of mycelial polysaccharides from Cordyceps sinensis on the expression of IL-6 and inos mrna in liver tissues of mice with immunological liver injury P<.1 compared with control group; P<.1, P<.5 3 inos [9-11] BCG LPS [12] [13] [14] [15] BCG LPS BCG T LPS

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