Vol. 42 No Journal of Jiangxi Normal University Natural Science Jul. 2018

Transcript

1 42 4 Vol 42 No Journal of Jiangxi Normal UniversityNatural Science Jul β - 1, 1,2*, β- - 4 ml 5 ml 10 ml ph 7 NaCl 200 μl 200 μl 5 min μl nm β- β- O 657 A DOI /j cnki issn β- β-carotene β- - β- 1-3 β- A β β- 9 β UV DHG-9076A 18 β- PHS-3C ph TDL80-2B KJT NXSFZ- DA1812 NXSFZD1715 HG jiangpingcao@ 126 com

2 4 - β g 10 ml 1 g ml min r min min ml6 mol L - 1 ph ml 100 ~ 300 μl 10 ml 5 ml 1b 100 μl NaCl 200 μl μl 5 min μl rpm 4 min 200 μl 5 min 200 μl 200 μl μl - 2 XMTD-204 FA2204B 2 1 FW200A 10 μg ml - 1 β- KQ-5200E 200 ~ 800 nm UPDR-II-20L β- 460 nm nm SFO-DLLME β- 5 0 mg 1 μg ml ml 5 5 ml 1 0 mg ml β mg ml - 1 SFO-DLLME β μl 5 ml μg E R = C s V s /C w V w 100% C s ml - 1 β- 2 β- μg 1 mg ml - 1 β- ml - 1 V s mlc w 25 μl 25 ml β- μg ml - 1 V w 1 0 μg ml - 1 3KOH ml KOH 100 g ml 100 ml 10 ml 5 ml 1 g ml - 1 KOH 100 μl 100 μl g 250 ml 50 ml 1a 1 a b

3 ph β ml 5 ml 5% 10% 20% NaCl 200 μl 4 4 NaCl 2 2 NaCl 88 3% ph ph r > R SD 4 5% ph R SD 1 8% 3 3 ph 5 0% 3 1 β- 0 5 μg g μg g - 1 ph 90 1% ~ 96 2% 95 5% ~ 103 0% R SD 1 4% ~ 4 5% β μg g - 1 β- 3 ph 90% β- 1 ~ μg ml - 1 Y = X + 1 / / / μg g - 1 ± R SD % n = 3μg g - 1 μg g - 1 ± R SD % n = ± ± 2 6 S ± ± ± 4 1 S ± 3 31 S ± 1 86 / % ± R SD % n = ± ± ± ± ± ± ± ± 3 5

4 4 - β HPLC - J β β- 10 GB v v= 4 ml β- S 5 ml ph 7 NaCl 200 μl 200 μl 11Hosotani K Kitagawa M Improved simultaneous determination method of β-carotene and retinol with saponification in human serum and rat liver J Journal of Chromatography B % - 12 J Roura S M D Piedra J Miravet S et al Beta-carotene reduces oxidative stress improves glutathione metabolism and modifies antioxidant defense systems in lead-exposed Engineering Science β- J workers J Toxicology and Applied Pharmacology Zaghdoudi K Pontvianne S Framboisier X et al Acceler- 2 J ated solvent extraction of carotenoids fromtunisian Kaki Teng Yuning Sheu M J Hsieh Y W et al β-carotene reverses multidrug resistant cancer cells by selectively modulating human P-glycoprotein function J Phytomedicine International Journal of Phytotherapy and Phytopharmacology Jin Qing Cheng HaijianWan Fachun et al Effects of feeding β-carotene on levels of β-carotene and vitamin A in blood and tissues of beef cattle and the effects on beef quality J Meat Science J Sharif H R Goff H D Majeed H et al Physicochemical stability of β-carotene and α-tocopherol enriched nanoemulsionsinfluence of carrier oil emulsifier and antioxidant J Colloids and Surfaces A Physicochemical and Engineering Aspects β- Technology α- J CO 2 β- J β- J Chumnanpaisont N Niamnuy C Devahastin S Mathematical model for continuous and intermittent microwave-assisted extraction of bioactive compound from plant material extraction of β-carotene from carrot peels J Chemical Diospyros kaki L peachprunus persica L and apricotprunus armeniaca L J Food Chemistry Bashipour F Ghoreishi S M Experimental optimization of supercritical extraction of β-carotene from Aloe barbadensis Miller via genetic algorithm J Journal of Supercritical Fluids Iftikhar Tan H J Zhao Y P Enrichment of β-carotene from palm oil using supercritical carbon dioxide pretreatmentsolvent extraction technique J LWT-Food Science and 18Xu Hui Jia Li Capillary liquid chromatographic analysis of fat-soluble vitamins and beta-carotene in combination with in-tube solid-phase microextraction J Journal of Chromatography B J

5 The Extraction of β-carotene in Millet Using Dispersive Liquid-Liquid Microextraction Combined with Solidified Floating Organic Droplets WANG Xuefeng 1 2 WANG Yali 1 2 ZHAO Wenxia 1 2 YAN Haiyan 1 CAO Jiangping 1 DI Hongwei 1 2 LIANG Yongfeng 1 2 XIE Qilong 3 2* 1 College of Chemistry and Chemical Engineering Ningxia Normal University Guyuan Ningxia China 2 Engineering and Technology Research Center of Liupanshan Resources Ningxia Normal University Guyuan Ningxia China 3 State Key Laboratory of Coal ConversionInstitute of Coal Chemistry Chinese Academy of Sciences Taiyuan Shanxi China AbstractA novel method for the extraction and determination of β-carotene in millet has been developed by dispersive liquid-liquid microextraction combined with solidified floating organic dropletssfo-dllmeand ultraviolet visible spectrophotometer The results show that the optimal parameters are diluting 4 ml of saponified solution with 5 ml water adjusting ph = 7 adding saturated NaCl and 200 μl 1-dodecanol extractantwith the same volume of acetonitriledispersantto the aqueous solution for extracting After that the centrifuge tube is immersed in an icewater bath for 5 min the floated organic solvent is solidified on top of the surface of the aqueous solution after a short period of time and any liquid phase is removed using the same syringe The solidified floating is quickly melted at room temperature and the extract is diluted to μl using THF Ultimately β-carotene in the extract is determined by ultraviolet visible spectrophotometer The advantage of the extraction method shows simplicity of the procedure high extraction rate which is available for the extraction and determination of β-carotene in millet Key wordssolidified floating organic dropletsdispersive liquid-liquid microextractionβ-carotene ( 418 ) The Preparation and Photoluminescence Properties of Europium Thenoyltrifluoroacetone Coordination Polymer Nanoparticles and Their Film ZHAO Zhipeng ZENG Chenghui SHUAI Min LIU Ran ZHONG Shengliang * College of Chemical and Chemistry Engineering Jiangxi Normal University Nanchang Jiangxi China AbstractEuropium thenoyltrifluoroacetone coordination polymereu-cpnanoparticles have been prepared via a facile solvothermal method employing EuNO 3 3 6H 2 O as metal ion source and thiophene-formyl trifluoroacetone as organic ligand In order to study the structure and composition of precursors a series of characterizations of the precursors are performed and their luminescence properties are studied In addition a series of spin-coating films of Eu-CP nanoparticles with different raw material ratios are prepared by mixing with polymethyl methacrylate and using N N-dimethylformamide as solvent And their fluorescence intensities are compared Furthermore light stability and thermal stability of the films obtained are also investigated Key wordscoordination polymerrare earththenoyltrifluoroacetonenanoparticlesfilms