EPS. DOI / j. issn EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM ACTIVATED SLUDGE IN MEMBRANE BIOREACTOR

Transcript

1 0 * EPS MBR B-EPS NaOH + 6 B-EPS B-EPS B-EPS B-EPS NaOH + B-EPS B-EPS B-EPS DOI / j. issn EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM ACTIVATED SLUDGE IN MEMBRANE BIOREACTOR Li Jihong Shan Shiliang Li Liang Hu Xiaomin Dong Yihua 2. College of Resources and Civil EngineeringNortheastern UniversityShenyang 0004China 2. School of Biology and Environment EngineeringShenyang UniversityShenyang 0044China Abstract Taking the content of proteinpolysaccharide and nucleic acid as measurement index for bound extracellular polymeric substances B-EPS total quantitythe extraction of B-EPS from activated sludge in the membrane bioreactor MBR was studied by six methods including thermal treatmentultrasonicationnaoh + formaldehyde treatmentsulfuric acid treatmenthigh speed centrifugation and steam treatment. The optimal extraction conditions were determined by comparative analysis of proteinpolysaccharide and nucleic acid extraction amount. The results show that ultrasonicationhigh speed centrifugationsteam treatment and sulfuric acid treatment have a low performance on B-EPS extraction. NaOH + formaldehyde treatment is effectivebut causes measurement deviation and effects operation process. Thermal treatment is determined as the optimal extraction method with high extraction efficiency of B-EPS little cell disruption and simple process. Keywords membrane bioreactor extracellular polymeric substances extraction protein 0 membrane bioreactormbr EPS 3 EPS MBR 70% ~ 80% 5 EPS 0-2 MBR * extracellular polymeric substances

2 EPS 2 EPS bound EPS B- EPS EPS soluble EPS S-EPS S-EPS B-EPS B-EPS EPS B-EPS r / min 0 min NaOH B-EPS B-EPS EPS 2 3 B-EPS NaOH + 6 MBR B-EPS. MBR 0. 2 m m m 4. 5 L B-EPS PVDF 20 B-EPS Folin- Lowry 2 22 Longer pump YE55x 8 min 2 min 253Y h ACO-38 LZB-6 EPS 0. m 3 / h ρ DO 4. 8 mg / L ml 4. 4 EPS mg / L 50 ml B-EPS B-EPS 2.. B-EPS min r / 5 min μm

3 2 3 3 B-EPS 5 ml W 3 B-EPS 2 min r / min 5 min μm EPS B-EPS B-EPS 5 ml min r / min 5 min μm 5 DNA 4 4 B-EPS B-EPS DNA 40 min B-EPS min B-EPS mg / g mg / g mg / g 2. 2 B-EPS EPS 4 40 min EPS B-EPS 40 min B-EPS B-EPS 50 W 2 min B-EPS mg / g mg / g DNA mg / g 2. 3 NaOH + B-EPS

4 mol / L NaOH 0 ml 30 ± 00 r / min 3 h r / min ph B-EPS 5 min μm EPS EPS 6 29 NaOH B-EPS 2. 4 B-EPS r / min 20 0% 20 min h. 5 h 6 NaOH B-EPS 2 6 NaOH. 5 mol / L mg / g NaOH B-EPS ph NaOH NaOH ml NaOH ml 2 B-EPS B-EPS 7 NaOH + > > 7 B-EPS 2. 5 B-EPS 2 B-EPS mg / g > > > NaOH + > > > > > > > > > NaOH + B-EPS EPS EPS King Forster EPS 30 NaOH + 7 2% B-EPS mg / g NaOH Wingender 28 + NaOH B-EPS

5 4 3 B-EPS min 2 B-EPS 50 W 2 min 3. 5 mol / L NaOH B-EPS NaOH J 2% B-EPS 4 3 B-EPS 6. J J Frolund BGriebe TNielsen P H. Enzymatic activity in the 2. activated-sludge floc matrix J. Applied Microbiology and J Biotechnology Willianms M DPirbazari M. Membrane bioreactor process for removing biodegradable organic matter from water J. Water Research J Seung H B Krishna R P Hyung J K. Lab-scale study of an anaerobic membrane bioreactor An MBR for dilute municipal wastewater treatmentj. Biotechnology and Bioprocess Engineering MBR J MBR J. 8 Konovalova VNigmatullin RDmytrenko Get al. Spatial sequencing of microbial reduction of chromate and nitrate in membrane bioreactorj. Bioprocess Biosystem Engineering J. substances EPS of sludgesj. Biotechnology MBR 0. J J Hernandez Rojas M E Van Kaam R Schetrite S et al. Role and variations of supernatant compounds in submerged membrane bioreactor foulingj. Desalination MBR J Frolund B Palmgren R Keiding K et al. Extraction of extracellular polymers from activated sludge using cation exchange resinj. Water Science and Technology EPS MBR J Houghton J I Stephensen T. Effect of influent organic content on digested sludge extracellular polymer content and dewaterability J. Water Research J D abzac PBordas FVan Hullebusch Eet al. Extraction of extracellular polymeric substances EPS from anaerobic granular sludge comparison of chemical and physical extraction protocolsj. Applied Microbiology and Biotechnology EPS J EPS 22Gaudy A F. Colorimetric determination of protein and carbohydrate J. Indian Water Wastes M. 24. M J J Zhang X Q Paul L B Brain K K. Comparison of extraction methods for quantifying extracellular polymers in biofilmsj. Water Science and Technology Wingender JNeu T RFlemming H C. Microbial extracellular polymeric substances characterizationstructures and function M. Berlin Springer Liu HFang Herbert H P. Extraction of extracellular polymeric # harvesttime@ 63. com