ID 32 STREP 07924D - CN /04 ID 32 STREP NIN FB 2-8 VP A NIN FB FB VP A VP B ( ) 2 FB ( ) () 5 3 NIN ( ) NCCLS M29-T2:CDC, NCC

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1 REF D - CN /04 Rapid ID 32 STREP 4 ID 32 STREP 4 ID 32 STREP 32 4 ATB API ID 32 STREP 25 ID 32 STREP ml(70 600) 3ml(#70 630)( ATB ) NIN (#70 490)FB (#70 560) VP A(#70 570) VP B(#70 580) (#70 610) ATB (#99 040)(#15 740) Tip (#15 710) (99 234) ATB (#70 900) ATB Express API (#70 200) 37 2ml 3ml FB 5ml NIN 5ml VP A 5ml VP B 5ml BB0.1% 0.35g g 100ml R61 R20/21/22 R36 S53 S45 7g ml R60 R61 R10 R20/21/22 S53 S45 KOH 20g 100ml R35 S2 S26 S27 S37/S39-12g 100ml R60 R61 R10 R20/21/22 S53 S45-1

2 ID 32 STREP 07924D - CN /04 ID 32 STREP NIN FB 2-8 VP A NIN FB FB VP A VP B ( ) 2 FB ( ) () 5 3 NIN ( ) NCCLS M29-T2:CDC, NCCLS M29-T2 2 CDC (a B)( ) Columbia () B 48-2

3 ID 32 STREP 07924D - CN /04 2ml ( ATB 3m1) No30 (4 ) ( No30) Tip ATB 55 l ATB (55 l) ( 0) B B 3 9 SACLARADARL1.C1.D 1.E 10 MBDGTAGBMAN0.C0.D 0.E 11 CDEXURE1.F0.F VP (0.0 ) VP A VP B APPA GTA( ) FB HIP (0.6 ) NIN 5 ( 10 ) ATB Express API - 3

4 ID 32 STREP 07924D - CN /04 ADH βgluβgarβgurαgal PAL RIB MAN SOR LAC TRE RAF SAC LARA DARL CDEX VP APPA βgal PYRA βnag GTA HIP GLYG PUL MAL MEL MLZ MBDG TAG βman URE V V V 3. V V 4. * V 1 (Streptococcus equi ssp equi)atcc (Erysipeiothrix rhusiopathiae)atcc Enterococcus aviumatcc Listeria grayiatcc Streptococcus agalactiaeatcc Streptococcus saccharolytiousatcc Streptococcus vestibularisatcc Tip ID 32 STREP ID 32 STREP (Columbia ) diode No.30( ATB ExpressAPI )4 ATB55 l (ATB ExpressAPI ) (4 5 ) ( 51 0 ) - 4

5 / 1.0 ADH / 1.1 βglu / 1.2 βgar 1.3 βgur / 1.4 αgal α 1.5 PAL 1.6 RIB (Acidification) 1.7 MAN (Acidification) 1.8 SOR (Acidification) 1.9 LAC (Acidification) 1.A TRE (Acidification) 1.B RAF (Acidification) / 1.C SAC SACcharose/(Acidification) 1.D LARA L(Acidification) 1.E DARL D(Acidification) 1.F CDEX (Acidification) 0.0 VP 0.1 APPA βgal 0.3 PyrA 0.4 βnag N- 0.5 GTA HIP 0.7 GLYG (Acidification) 0.8 PUL (Acidification) 0.9 MAL (Acidification) VP A VP B / 5 < 10 FB / 5 < 10 (APPA GTA) NIN / 5 < 10 0.A MEL (Acidification) 0.B MLZ (Acidification) / 0.C MBDG -B-D-(Acidification) 0.D TAG (Acidification) 0.E βman 0.F URE p. I p. II p. III V

6 Columbia blood Diode / Diodo / Díodo 30 Suspension Medium 55 µl rapid ID 32 STREP 4:00 37 rapid ID 32 STREP VP : VP A VP B APPA GTA : FB HIP : NIN VI

7 ID 32 STREP ( 37 C 24 ) RAPID ID 32 STREP V2.0 ADH BGLU BGAR BGUR AGAL PAL RIB MAN SOR LAC TRE RAF VP APPA BGAL PYRA BNAG GTA HIP GLYG PUL MAL MEL MLZ SAC LARA DARL MBDG TAG BMAN CDEX URE Abiotrophia adiacens Abiotrophia defectiva Aerococcus viridans Alloiococcus otitis Enterococcus avium Enterococcus casseliflavus Enterococcus durans Enterococcus faecalis Enterococcus faecium Enterococcus faecium Enterococcus gallinarum Enterococcus hirae Enterococcus saccharolyticus Erysipelothrix rhusiopathiae Gardnerella vaginalis Gemella haemolysans Gemella morbillorum Lactococcus garvieae Lactococcus lactis ssp cremoris Lactococcus lactis ssp lactis Lactococcus raffinolactis Leuconostoc spp Listeria grayi Listeria spp Streptococcus acidominimus Streptococcus agalactiae Streptococcus alactolyticus Streptococcus anginosus Streptococcus bovis I Streptococcus bovis II Streptococcus bovis II Streptococcus canis Streptococcus constellatus Streptococcus downei/sobrinus Streptococcus dysgalactiae ssp dysgalactiae Streptococcus dysgalactiae ssp equisimilis Streptococcus equi ssp equi Streptococcus equi ssp zooepidemicus Streptococcus equinus Streptococcus gordonii Streptococcus group L Streptococcus intermedius Streptococcus mitis Streptococcus mitis Streptococcus mutans Streptococcus oralis Streptococcus oralis Streptococcus oralis Streptococcus parasanguis Streptococcus pneumoniae Streptococcus porcinus Streptococcus pyogenes Streptococcus salivarius ssp salivarius Streptococcus salivarius ssp thermophilus Streptococcus sanguis Streptococcus sanguis Streptococcus suis I Streptococcus suis II Streptococcus uberis Streptococcus uberis Streptococcus vestibularis II

8 ID 32 STREP 07924D - XL - CN /04 1. DESMONCEAUX M., GUICHERD M., FAGET N., ALLARD F., BOEUFGRAS JM., MONGET D. rapid ID 32 Strep, a New Identification System for Streptococci and Related Genera. (1992) Zbl. Bakt., suppl. 22, FRENEY J., BLAND S., ETIENNE J., DESMONCEAUX M., BOEUFGRAS JM., FLEURETTE J. Description and Evaluation of the Semiautomated 4-Hour rapid ID 32 STREP Method for Identification of Streptococci and Members of Related Genera. (1992) J. Clin. Microbiol. p N HARDIE JM., WHILEY R.A., FRASER H., BEIGHTON D. Identification of Viridans Streptococci by the API rapid ID 32 Strep test kit. (1991) 5th European congress of Clinical Microbiology and Infectious Diseases, Oslo (Norway). 4. MURRAY P.R., BARON E.J., PFALLER M.A., TENOVER F.C., YOLKEN R.H. Manual of Clinical Microbiology. Sixth Edition. (1995) American Society for Microbiology, Washington, D.C. 5. SCHLEIFER K.H., KILPER-BAELZ R. Molecular and Chemotaxonomic Approaches to the Classification of Streptococci, Enterococci and Lactococci : A review. (1987) System, Appl. Microbiol., 10, SNEATH P.H.A., NAIR N.S., SHAPE M.E., HOLT J.G. Bergey's Manual of Systematic Bacteriology Ninth Edition, Vol. 2 (1986) Williams and Wilkins Co, Baltimore, Md. au capital de F RCS LYON Marcy-I'Etoile / France : (33) / : (33) biomérieux, Inc. 595 Anglum Road, Hazelwood, Missouri / USA : (1) / : (1)