Abstract : With the rapid development of genetically modified ( GM) crops, a number of fundamental issues regarding health

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1 473 1, ( ; 21, ) :,,,, :, ;; Progress in Rodent Models Used for Detecting the Allergenicity of Genetically Modified Foods SUN Na2na, PENG Yu2fa, J IA Xu2dong, Li Ning ( Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing , China) Abstract : With the rapid development of genetically modified ( GM) crops, a number of fundamental issues regarding health concerns have been raised. The potential allergenicity of GM foods is one of the focuses of attention. Recently, the development of animal models used for the assessment of allergenicity of GM crops has become a hot field of research and development. Several rodent models used for detecting the allergenicity of genetically modified foods were reviewed. Key words : Food, Genetically Modified ;Allergenicity Assessment ;Rodent Models Π, 1996,, 2S,, [1, 2 ],, [2 ], ( IFBC) ( ILSI) 1996 (decision tree approach) [3 ], (FAO) (WHO) [4, 5 ],, 1 (food allergy), :973 (2007CB109207) ; (2008ZX ) : :, ( adverse reactions to food), (food intolerance) [6 ] 8 % 2 %4 % 315 kda IgE, [7 ], IgE I,,,CD4 + Th 2, IL24 IL213,Th 2 IL24 IL213 Th 1 IFN2 IL22 () IgE Th 2 (mast cell, MC) ( basophil, Bas) [8 ] IgE MC Bas,, IgE,,,,,, [9 ] CD8 + Tc

2 474 CHINESE JOURNAL OF FOOD HYGIENE IgE [8 ] 2 :, ;,, ; IgE Th 2 ; ; BALBΠc,C3HΠHeJ,,,, [10] 211 (Brown Norway, BN) BN (IgE),,BN,, BN, [11 ],BN, [12 ] [13-17 ], KNIPPELS [14 ] (OVA),( mg OVA), () (,1 2,1 1,2 1 ) 42 BN OVA (1 mgπd),( ELISA) ( PCA), OVA IgE 80 % OVA IgE 2835, KNIPPELS [16 ] Wistar Piebald Virol Glaxo ( PVG) Hooded Lister (HL) BN OVA, BN OVA IgE, BN,KNIPPELS [15 ] BN BN OVA OVA BN, - ( 2lactoglobulin),,OVA OVA BN, BN [18 ] BN 1 mgπd OVA ( PAP) 10 mgπd (HEWP),BN OVA HEWP, PAP,BN [19 ] BN,,BN, 212 BALBΠc,BALBΠc,IgE, BN, Th 2 IgE [20, 21 ] DEARMAN [22, 23 ] BALBΠc, KIMBER [24 ] BALBΠc IgG IgE BALBΠc IgE ( inherent sensitizing potential), (immunogenic proteins) ( potentially allergenic proteins) IgG IgE,IgG IgE [22, 23, ], : ( ) [8, 28 ], IgE,,,, BALBΠc DEARMAN [27, 29 ] 012 % 2 %OVA 20 % PAP 0125 ml

3 475 BALBΠc, PCA IgE,, 32 IgE ; OVA16 IgE ; PAP IgE,BALBΠ c [30 ] 2 %OVA (BSA) ( TI) PAP 0125 ml,,ova TI, BSA PAP BALBΠc,, BALBΠc IgE, 213 C3HΠHeJ C3HΠHeJ IgE, [31-33 ] BOWMAN [37 ] C3HΠHeJ BALBΠc,,, C3HΠHeJ,BALBΠc 68 C3HΠHeJ (food total protein), ( ),C3HΠHeJ [33, 34 ], +,TLR, IgE, [34 ] (1 mgπ 2 mgπ) + C3HΠHeJ Toll 4 ( Toll2like receptor 4, TLR4), (LPS) LI BASHIR [32, 35 ] TLR4 CAPOBIANCO [34 ] ( ST) + (cholera toxin, CT) 8 C3HΠHeJ mrna, IL213mRNA,IL24mRNA 5,IL24mRNA, IFN2 IL2 10,Th 2, ), [29 ], [31 ] LI [31, 32 ],,, 3 C3HΠHeJ ( mgπg BW) + CT ( 013g CTΠg BW), 5 C3HΠHeJ ( 1 mgπ, 5 mgπ ) + CT ( 10g CTΠ) 110 mgπg BW 1 mgπ PROUST [36 ], C3HΠHeJ, 4, 14 IgE, IgE, (10g CT), IgE,, IgE, IgE,, (5 mgπ+ 10g), IgE IgE, (,, [24 ],C3HΠHeJ

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