7 PTEN p Tsuchiya DNA Hepatocyte nuclear factor beta HNF beta HNFbeta IGFBP GLUT G Pase MODY maturity onset diabetes of the young Tsuchiya HNFbeta HNFbeta HNFbeta sirna CPT HNFbeta HNFbeta TOVG KOC ESMCAS HNF beta Real time quantitative RT PCR QIAGEN HiPer- Fect Transfection Reagent sirna QIAGEN RT PCR RNA Affymetrix GeneChip Expression Array HNFbeta SPP UGT AANXA
HNFbeta 7 Relative Expression of HNF beta mrna.6 TOV2G KOC ES 2 MCAS Ovarian cancer cell lines HNFbeta Real -time quantitive RT PCR TOVG, KOC, ES clear cell carcinoma MCAS mucinous cyst adenocarcinoma RNA.2.8.6.4.2 TOV 2G.2.8.6.4.2 KOC control sirna control sirna TOVG,KOC HNFbeta BCL LCFLAR CCND HNFbeta Real -time quantitative RT PCR poly- HEMA MTS Invasion assay Matrigel invasion chambers TUNEL assay CPT sirna CPT h MTS HNFbeta Real time quantitative RT PCR TOVG KOC MCAS ES HNFbeta TOV G KOC TOVG RT PCR HNFbeta Real time quantitative RT
72 SPP UGT A ANXA 4.2.2.2.8.8.8.6.6.6.4.4.4.2.2.2 control sirna control sirna control sirna p.5 Survival rate 8 6 4 2 p.5 control cells knockdown cells BCL 2 L CFLAR CCND.2.2.2.8.6.4.2.8.6.4.2 control sirna control sirna control sirna p.5.8.6.4.2 Invasion assay PCR SPP UGT A ANXA BCL L CFLAR CCND survival rate invasion assay TUNEL assay CPT HNFbeta IGFBP GLUT G Pase HNFbeta HNF beta Tsuchiya HNF beta HNFbeta
HNFbeta 73 TUNEL assay sirna HNFbeta HNFbeta HNFbeta sirna HNFbeta HNFbeta SPP CFLAR BCL LCCNDUGT A ANXA HNF beta HNFbeta Cell Viability 2 8 6 4 2 2 5 2 3 4 5M sirna control CPT MTS HNFbeta Vercellini P et al. Endometriosis and ovarian cancer. Am J Obstet Gynecol McMeekin DS et al. Endometrioid adenocarcinoma of the ovary and its relationship to endometriosis Gynecol Oncol Ogawa S et al. Ovarian endometriosis associated with ovarian carcinoma : a clinicopathological and immunohistochemical study. Gynecol Oncol Prowse AH et al. Molecular genetic evidence that endometriosis is a precursor of ovarian cancer. Int J Cancer Jimbo H et al. Prevalence of ovarian endometriosis in epithelial ovarian cancer. Int J Gynaecol Obstet
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