ISSN 100727626 CN 1123870ΠQ 2007 10 Chinese Journal of Biochemistry and Molecular Biology 23 (10) :823 828 DAZ BOUL E 3 3,,,,, ( 210095) BOULE 2001 DAZ BOUL E BOULE BOULE DAZ DAZ RNA (RBM) DAZ DAZL DAZ 3 BOULE BOULE ;DAZ ; R69812 Structure and Function of BOULE Protein, a Ne w Member of the Human DAZ Family LI Qi2Fa 3, LI Yin2Xia, ZHANG Qing2Bo, LIU Zhen2Shan, QU Xu2Guang, XIE Zhuang 3 ( Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China) Abstract BOULE protein, a new member of DAZ family, is found in 2001, and is the key regulation factor in meiosis of human spermatogenesis The expression change of BOUL E gene or the absence of BOULE protein brings on meiosis arrest and spermatogenic failure, which can lead to azoospermia and male infertility Primary structure of BOULE protein owns the characterized domain of DAZ family, including DAZ repeat and RNA2 binding domain ( RBM), so BOULE protein is list the third member followed the DAZ and DAZL In this papper, we summarized the finding, structure and location and focused on introducing in detail its function and mechanism in germ cell meiosis in male and other species Key words BOULE protein ; DAZ family ; meiosis arrest ; male infertility A DAZ DA Z B [11 ], DA ZL [14 ] [15 ] [16 ] [17 ] 3, :2007206204 ; :2007208227 (No 30500360) [1 3 ] DA Z ( Deleted in Azoospermia) ( DA Z [4 family) ] DA Z 3 1 Y DA Z [5 ] 2 DA ZL ( deleted in (No KJ05011) 3 :Tel : 025284395046, azoospermia2like) [6 ] BOUL E [7 ] 3 RNA E2mail : liqifa @njau edu cn ; E2mail : zxie @njau edu cn Received : June 4, 2007 ;Accepted :August 27,2007 Supported by National Natural Science Foundation of China (No 30671503) and Youth Science and Technology Innovation Foundation of Nanjing Agriculture University (No KJ05011) 3 Corresponding author [8,9 ] [10 ] Li Qi2Fa Tel : (025) 84395046, E2mail : liqifa @njau edu cn Xie Zhuang Tel : (025) 84395046, E2mail : zxie @njau edu cn [12 ] [13 ]
824 23 [7, BOUL E ] [18 ] [19 ] 325 bp 872 bp 1 DAZ,, 283 (Fig 1) DA ZL Boule DA Z BOULE 2001 DAZ DAZL,BOULE RNA [7 ] ( 33 110 RNA 2 ) ( ribonucleoprotein, RNP ) RNP1, BOUL E RNP2 [5,6,24 ] DAZ RNA [4,25 BOULE ] ; 164 194 (meiotic arrest,ma) DAZ [25 ] [20 ], BOULE [20,21 ] BOULE Fig 1 Sketch map of primary structure of BOULE protein 1 BOULE 1996, Eberhart [18 ] (transcript variants) B1 B2 B3,3 N DA Z 3 1 Boule DA Z, Boule (alternatively spliced exons 1) RNA, 80 220 1 ; BOULE DAZ : (1) DAZ DAZL BOULE RNA ( RNA2binding domain,rbm) RNA RNA [7 ] 2 BOULE BOUL E 2 046 bp 849 bp 3113 kd [11 ] DAZ DAZ Kostova [21 ] 3 BOULE Boule,Boule 3 BOULE G 2 ΠM 311, 2001,Xu [7 ] BOUL E 2q33 [7 ] DA Z DAZΠDAZL Yq11123 [5 ],DAZL, DAZΠDAZL 3p24 [6 ], DA Z 1 DAZ DAZL Boule 2, Boule 312 (42 %) DAZ Northern, BOUL E DAZL Boule BOULE Western BOUL E 32 kd BOULE BOUL E [26 ] [6 ] [7 ] DA Z DA Z [5 ] DA ZL [6 ] [22 DAZ ] ; (2) DAZ DAZL BOULE ( ) DAZ (DAZ repeat) [23,DAZL ] ; (3) DAZ DAZL BOULE, boule [4 ] 3 313 BOULE DAZ DAZ 3, BOULE ( ) (leptotene spermatocytes) (zygotene spermatocytes)
10 :DAZ BOULE 825 (pachytene spermatocytes) (diplotene) ; ( round spermatids ) ( elongating, 12 spermatids), BOUL E (primordial germ cells) Boule Boule [4,27 ] ( spermatogonial cells),, Boule BOULE Boule, [24,25 ] BOULE BOUL E [25 ] [28 ] Boule, [7 ] Boule DAZ DAZL BOUL E Boule Boule [29,30 ] BOUL E BOULE 4 BOULE (metazoans) 411 41111 BOULE 412 BOUL E 315 10-4 BOULE, BOUL E ( ) RNA Xu [11 ] 164 BOUL E,BOULE 3, 3 (germ cell maturation) ( 3 2untranslated region, 3 2UTR ) [24 ], BOUL E Luetjens [25 ] 18 BOUL E, 2 11 [31 ] (MA) ( Westerveld [32 ] Lepretre [33 ] 2 ) BOUL E BOUL E 2 1 GΠC Q2E (mixed atrophy) BOUL E [32,33 BOUL E ] BOUL E Boule 1 BOULE twine ( Cdc25 [25 ], BOUL E ), Cdc2Π (testicular failure), B M (M phase2promoting BOUL E, factor,mpf) Cdc2Π B G 2 2 (sperm retrieval) BOUL E [20 ], BOUL E boule twine [34,35 ] Cdc25 2,BOULE Cdc25A Cdc25C, MPF [7,9,21 ] [36 G 2 2M ] Xu [11 ], 41112 BOULE BOUL E boule Xu [11 ] BOUL E Boule M 1 BOULE Boule ( ) BOULE Boule? Boule BOUL E M
826 23 M MPF MPF, CDC25A B CDC2 (cell division cycle 2) MPF MPF,,BOULE,Wee1 Myt1 CDC25A CDC25 G 2 ΠM,BOULE CDC25A mrna [37 39 ] [25 3 CDC25, ] Xu [11 ] CDC25A, BOUL E boule [40,41 ] Boule RNA twine BOULE BOULE (Cdc25 ) Boule,CDC25A mrna, twine [ 11, 25] Fig 2 Pattern map of BOULE protein interaction mechanism in human The vertical arrows show positive regulation, P is the PO 2-3, mrna, BOULE ( Fig 2), Cheng [38 ], CDC25A :BOULE CDC25A mrna,cdc25a BOULE MPF CDC2 MPF RNAi ; MPF G 2 ΠM DAZΠDAZL PUMILIO2 DAZΠDAZL 5 BOULE,BOULE ( References) BOULE BOULE : BOUL E BOUL E [32,33 ], BOUL E? Luetjens [25 ],BOULE CDC25A 2 DAZAP1 DAZAP2 DZIP1 DZIP2 DZIP3 hqk3 [9 ], [ 1 ] Crackower M A, Kolas N K, Noguchi J, et al Essential role of Fkbp6 in male fertility and homologous chromosome pairing in meiosis [J ] Science, 2003, 300 (5623) : 129121295 [ 2 ] Miyamoto T, Hasuike S, Yogev L, et al Azoospermia in patients heterozygous for a mutation in SCP3 [J ] Lancet, 2003, 362 : 17142 1719 [ 3 ] Zhang W, Zhang S, Xiao C, et al Mutation screening of the FKBP6 gene and its association study with spermatogenic impairment in
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