45 2 ( ) Vol. 45 Sup. 2 2006 12 Journal of Xiamen University (Natural Science) Dec. 2006,,, 3 (,, 361005) :,. : ; ; : Q 45; S 917 : A : 043820479 (2006) S220170206,. 20,, ;.,, ( Penaeus m onodon) (L itopenaeus vannam ei) (M acrobrachium rosenberg ii) ( Hom a rus am ericanus),.,,. 1 1. 1 1. 1. 1,,,. (Ca llinectes sapidus) (S cylla serrata),.,, [ 1, 2 ]., (L ibin ia em arginata ) [ 2, 3 ]. ( Corpora allata), (Methyl farnesoate,mf) : 2006211215 : (40406030, 30300269) : (1970 - ),,,. 3 : zoopecol@ xmu. edu. cn,., MF, [ 4, 5 ]., [ 6 ].,,, MF MF [ 7 ]. Takac [ 8 ] MF, MF. MF MF. 1. 1. 2 Y Y,,. Y,. Y,,,.,,,,,,, [ 9 ]. Y., (A can tho2 nyx lunula tus), [ 10 ]., ( E riocheir sinensis) 202,, 202 ; 202 [ 11, 12 ]. Y, [ 13 ]. 1. 1. 3,
2 : 171..,. 3 [ 14 ]. (Holocrine) [ 15 ]. ( GSH )., GSH, (M arsupenaeus [ 16 ]. Lee [ 17 ] japonicus) GSH 1 2 ku [ 23 ], ( E riocheir japonicus), GSH 7 ku, [ 24 ].,, 3 5. ( FSH) 1. 1. 4 (LH), FSH LH,, GSH. 17 2 FSH LH [ 25 ]., [ 12 ]. 1. 2. 3 17 2, Fingerman, 52, 17 2 ; ( RPCH ) GSH / GIH 17 2., (U ca pugila ter) ;, 17,, 2 (Met2ENK) [ 18 ]. ( Portunus tritubercu latlus) GSH / GIH, 17 2 [ 26 ]. [ 19 ]. 52HT 1. 1. 5 [ 27 29 ]., 52HT,. 52HT. F [ 30, 31 ]. E (O ziotelphusa senex senex), D [ 20 ]. 1. 2 1. 2. 1,. X 2,.,,,. X, X. X (CHH s),, ( GIH) (M IH) (CHH ) (MO IH)., 71 78 ;,, CHH (CHH2p recursor2related pep tide, CPRP),. GIH,, (V IH) [ 21, 22 ]. GIH,.,. 1. 2. 2 2 2. 1,,., [ (L ipovitellin, LV) (V itellogenin, VG) ],., [ 32 ]. [ 33 ],,, ;., Glu, A sp Pro Gly A la Leu Lys A rg, Thr Ser Val Ile Tyr Phe H is,met ;, Gly A la Phe Tyr 4 ;,,,
172 ( ) 2006. Penaflorida [ 34 ] (HPLC).,,.,.,,. 2. 5, FAA,.,,. 2. 2,, ( EFA ) ( PL )., (HUFA s). [ 35 ] [ 36 ],, HUFA, 2%, ; HUFA ; HUFA ( EPA ). [ 37 ],,, HUFA EPA DHA [ 38 ].,,,.. 20: 5n3 22: 6n3 18: 2n6 18: 3n3,,, n32hufa /n62hufa,, [ 39 ].,,., /,, TAG.,, ; n32hufa, EPA.,,, HUFA [ 40 ].. [ 41 ] 40% 6%, 28,,, :, HSI, GSI, 1 2, 2 GSI 9. 15,, (Vg), 1 2,., GSI IV, IV GSI ( 11. 50) ( 11. 25) > (8. 85) > ( 7. 30). 6% 15%, HSI, 2 15% HSI 10. 56, (9. 78), ( 8. 83), 6% ; GSI. 15% GSI 11. 49, 9. 47 9. 23. 15%. 15%, 1. 394 10 3 cell/g mass, 77. 46% 21. 53% ; 1. 088 10 3 cell/g mass., 62. 11% 15. 03%, 15% (72. 48% ) 6%, (2. 216 10 3 cell/g mass) ( 96. 03% ).. 2. 3, A (V itam in A, V A ) E (V E ) C (V C ) 2., V A V E V C 2. [ 42, 43 ], V E V C HUFA,
2 : 173. [ 43 ], V C V C, ;,, V C. [ 45 ] V E 2 65 5 g., 2 V E ( p < 0. 05), V E 2, ( p < 0. 05), ( p < 0. 05). 2. 4,,, ;,, [ 46 ]. [ 44 ] 75 5 g,. : ( ) ( ) ( p < 0. 05), 5 mg/kg (0 mg/kg) ( p < 0. 05), 0. 5 mg/kg ( p > 0. 05). 2. 5,. M illamena & Quinitio [ 47 ],, ; D junaiah [ 48 ], (S cylla pa ram am osain). 3, : MF. GIH GSH,,., X2 M IH MO IH Y,.,. : GSH GSH ; ; ; ;..,.,.,,. :,,,,.,. ; ; ; ; ; ;,,. : [ 1 ] Yudin A L, D iener R A, Clark W H, et al. Mandibular gland of the crab, Callinectes sapidus[ J ]. B iol Bull, 1980, 159: 760-772. [ 2 ],,,. [ J ]., 2005, 27 (1) : 120-124. [ 3 ] H insch G W. The mandibular organ of the female sp ider crab, L ibinia em arginata, in immature, mature and ovigerous crabs[ J ]. J Morphol, 1981, 168: 181-172. [ 4 ] Laufer H, Borst D, Baker F C, et al. Identification of a juven2 ile hormone2like compound in a crustacean [ J ]. Science, 1987, 235: 202-205.
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2 : 175 113-119. [ 36 ]. [D ]. :, 2004. [ 37 ] W en X B, Chen L Q, A i C X, et al. Variation in lip id com2 position of Chinese m itten2handed crab, Eriocheir sinensis during ovarian maturation [ J ]. Comp B iochem Physiol, 2001, 130B (1) : 95-104. [ 38 ] W en X B, Chen L Q, Zhou Z L, et al. Rep roduction re2 sponse of Chinese m itten2handed crab ( Eriocheir sinensis) fed different sources of dietary lip id [ J ]. Comp B iochem Physiol, 2002, 131A (3) : 675-681. [ 39 ],,,. [ J ]., 2005, 20 (4) : 222-227. [ 40 ]. [D ]. :, 2005. [ 41 ],,,. [ J ]., 2001, 23 (3) : 66-77. [ 42 ],,,. C E [ J ]., 2003, 27 (1) : 46-54. [ 43 ],,,. C E HUFA [ J ]., 2002, 26 (6) : 533-541. [ 44 ],,,. C [ J ]., 2004, 39 (2) : 1-5. [ 45 ]. E 2 [D ]. :, 2005. [ 46 ],,,. [ J ]., 2003, 22 (2) : 255-262. [ 47 ] M illamena O M, Quinitio E. The effects of diets on rep ro2 ductive performance of eyestalk ablated and intact mud crab Scylla serrata [ J ]. Aquaculture, 2000, 181 (1 /2) : 81-90. [ 48 ] D junaidah I S, W ille M, Kontara E K, et al. Rep roductive performance and offsp ring quality in mud crab (Scylla para2 m am osain) broodstock fed different diets [ J ]. Aquaculture international, 2003, 11 (1 /2) : 3-15. Advances on Reproductive Physiology of Crabs YE Hai2hui, A I Chun2xiang, HUANG Hui2yang, L I Shao2jing 3 (Department of Oceanography, Institute of Subtrop ical Oceanography, Xiamen University, Xiamen 361005, China) Abstract: This paper reviews the current knowledge on the rep roductive endocrine and rep roductive nutrition of crabs. Additionally, the further studies on rep roductive physiology of crabs are suggested. Key words: crab; rep roductive endocrine; rep roductive nutrition