Figure S1. Localization of GiOR-1 with truncated N-terminal domain of 29 amino acid residues (ΔL-GiOR-1-HA).

Figure S1. Localization of GiOR-1 with truncated N-terminal domain of 29 amino acid residues (ΔL-GiOR-1-HA). A L-GiOR-1-HA Tom40 DAPI merge DIC B LYS CYT ORG ORG Trypsin - - - + L-GiOR-1-HA

Figure S2A. Phylogeny of hydrogenases, Hyd-CPR fusion proteins and Nar1 Mastigamoeba balamuthi H Blastocystis hominis H 100/1 Trichomonas vaginalis F Trichomonas vaginalis F 68 /1 Carpediemonas membranifera F Chilomastix cuspidata F Dysnectes brevis F Trimastix PCT F Hydrogenases and 75 Trichomonas vaginalis H Hyd-CPR fusion proteins /1 100 /1 Trichomonas vaginalis H Trichomonas vaginalis H HYD FMN FAD 66 /1 Chlamydomonas reinhardtii H Naegleria gruberi H HYD 88/ 73/ 0.99 0.96 Entamoeba histolytica H Giardia intestinalis H Spironucleus salmonicida H Spironucleus salmonicida H Encephalitozoon cuniculi 100/1 Chlamydomonas reinhardtii Arabidopsis thaliana 50/ 0.94 Mastigamoeba balamuthi 62 /0.58 Dictyostelium discoideum Tetrahymena thermophila Homo sapiens Saccharomyces cerevisiae Monosiga brevicollis Naegleria gruberi Cryptosporidium parvum Nar1 Blastocystis hominis Entamoeba histolytica 58/ 0.64 Carpediemonas membranifera Chilomastix cuspidata Dysnectes brevis 99/1 Giardia intestinalis 92/1 87/1 Spironucleus vortens Trepomonas Sp Spironucleus salmonicida 98/1 Bodo saltans 97/1 Leishmania major Trypanosoma brucei Trichomonas vaginalis 0.6 HYD

Figure S2B. Comparison of HC1 domains among hydrogenases (Hyd), hydrogenases that are fused with CPR (Hyd-CPR), and Nar1 components of CIA pathway. HC1 Naegleria gruberi Trichomonas vaginalis Giardia intestinalis Spironucleus salmonicida Blastocystis hominis Entamoeba histolytica Trimastix marina PCT Trichomonas vaginalis Trichomonas vaginalis Carpediemonas membranifera Chilomastix cuspidata Naegleria gruberi Bodo saltans Trichomonas vaginalis Carpediemonas membranifera Chilomastix cuspidata Dysnectes brevis Giardia intestinalis Spironucleus salmonicida Trepomonas PC1 Blastocystis hominis Entamoeba histolytica Saccharomyces cerevisiae MYTSCCPGWVNLVESDFPE - I I PNVSSCKSPQQMLGS- VI RN MFTSCCPAWVNYVEKSDPS -LI PHLSSCRSPMSMLSS- VI KN VFTSCCPAWVNLCEMKYPE - LLPQVSTAKSCVAMVATL- VKR VFTSCCPAWVNLCERKYPQ- LLGRLSTAKSPHGSI CT- LI KR MFTSCCPGWVNMAEKCYPE- LI PNLSSCRSPHMMLGSC- VKT AI TSCCPGWLEYLEKKYPD- LI PRVSTARSPI ACLAGC- I KN MFTSCCPGWI NLLEQKYPE - FI PNVSSCKSPQQMLGA- VI KT - FTSWCPAWVNFVEKLHPE -LIPNL STVKSPHI L LSV- I I RN MI TSS CPSWVNFI EKI FPD- LTENL STTMSPHI I TGS- LI KN MFTSCCPAWI NNI ETRFPD- YLDNLSTARSPMMMMGS- VVKT MFTSCCPGWI NFVEQEAPD- FI PNLSSCKSPMGMQSAL- LRT VFASACPGWI CYAEKTQPE - I I PSI STVKSPQQI MGT- FVKK MI VSS CPGWVCYCEKQYPE- LLPHLSRVMSPQGI AGS- LLKR - MI SRCAGSVVYYERKTS- - YADHLAQI KPYPQLYAMYEKKI LVVSS CPGFVCVCEKQNPE - VLPKMSRVGSPMAYTAA- ---- LFVSACPGWVCLAQKRYPD- TVARLARAQSSMTVFGTALLAR RI VTVCPAVRLYI GKRGPS -LIPHLDNTPSPTEI AGHGL - - - AI I THCPAVRLFI TKRNRE- LI SYI VSTASPMELFGASYCGI --I THCPAVRLYI LKKKPE -LYKYVNTVGSMLEFQ------- LI VSHCPAAKLFLNKKDS S -LSKFFSKSASPLEI FFS- - - - - FI TSNCPGWVCYAEKKTHA- LVPFLSTTKSAQQI MGS- LLRK - LTST CPGWVCYSEKMQGKWMFEYMSKVASSMTI AGMI - MKK LLSAVCPGFLI YTEKTKPQ- LVPMLLNVKSPQQI TGS- LI RA Hyd Hyd-CPR Nar1

Figure S3. Distribution of Tah18, Dre2, RNR1 and RNR2 proteins in Metamonada and various anaerobes. Tah18 Dre2 RNR1 RNR2 N. gruberi T. brucei B. saltans T.marina PCT T. vaginalis C. membranifera E. cyprinoides CLO NY171 C. cuspidata Excavata Anaerobes G. intestinalis S. salmonicida S. vortens Trepomonas PC1 B. hominis E. histolytica M. balamuthi D. dictyostelium D. brevis Amoebas

Figure Legends Figure S1. Localization of GiOR-1 with truncated N-terminal domain of 29 amino acid residues (ΔL-GiOR-1-HA). (A) Immunofluorescence microscopy revealed that ΔL-GiOR-1- HA localizes to mitosomes. ΔL-GiOR-1-HA was detected using anti hemagglutinin (HA) antibody (green). Tom40 was detected as mitosomal marker protein using polyclonal rabbit anti-tom40 antibody (red). Nuclei were visualized by DAPI (4,6-Diamidine-2 -phenylindole dihydrochloride). DIC, differential interference contrast. (B) Alternatively, the cellular localization of ΔL-GiOR-1-HA was investigated using western blot analysis of cellular fractions (LYS, cell lysate; CYT, the cytosol; ORG, the organellar fraction) and protein protection assay (treatment of the organellar fraction with 200 μg/ml of trypsin for 10 min at 37 C). Figure S2. Phylogeny of hydrogenase like domain containing proteins A. Maximum likelihood-based phylogeny of Nar1 and hydrogenase domain-containing proteins in eukaryotes. Hydrogenases cluster together with the CPR-hydrogenase fusion from metamonads. Numbers indicate statistical support in the form of bootstrap values or PP values. The scale bar represents the estimated number of amino acid substitutions per site. Only bootstrap support values greater than 50 and PP values greater than 0.5 are shown. Green indicates Viridiplantae, red Opisthokonta, yellow the SAR supergroup, brown Amoebozoa, and blue Excavata. H, only hydrogenase domain-containing protein; F, CPRhydrogenase fusion protein. B. Alignment of HC1 site of CPR-hydrogenase fusion proteins in metamonads with representative protein sequences for hydrogenase and nar1 protein. The HC1 motif is highlighted.

Figure S3. Distribution of Tah18, Dre2, RNR1 and RNR2 proteins in Metamonada and various anaerobes. A function in the synthesis of diferric-tyrosyl radical (Fe(III)2-Y ) cofactor of ribonucleotide reductase (RNR) was recently suggested for Tah18 and Dre2 (17). Hence, we searched to determine whether genes for RNR proteins are missing in organisms lacking Tah18-Dre2 proteins. Nevertheless, we failed to find any such correlation to support this hypothesis. RNR1, Ribonucleotide reductase 1; RNR2, Ribonucleotide reductase 2. Black shading, an ortholog is present; White, an ortholog is absent from the genomic database; Grey, an ortholog is absent from a given EST database.

Table S1. Analysis of immunoprecipitated proteins by mass spectrometry Identified proteins Acc. Number Molecular weight Protein identification probability Total unique spectra count GiOR1 Cia2 CBirA CBirA CBirA CBirA Wt Wt GiOR1 Cia2 CBirA CBirA CBirA CBirA Wt Wt Nitric oxide synthase, inducible location=glchr04:846624 848438( ) length=604 GL50803_91252 67 kda 100% 7 Chaperone protein DnaK HSP70 location=glchr03:1227021 1228943( ) length=640 GL50803_14581 70 kda 100% 9 Chaperonin 60 location=glchr02:250593 252236( ) length=547 GL50803_103891 57 kda 100% 8 Cysteine desulfurase location=glchr03:1250752 1252053(+) length=433 GL50803_14519 48 kda 100% 4 Alpha mitochondrial protein peptidase like protein location=glchr01:975015 976232( GL50803_9478 45 kda 100% 3 Glutaredoxin related protein location=glchr01:1150793 1151401(+) length=202 GL50803_2013 22 kda 100% 3 Hypothetical protein location=glchr05:1420047 1420907( ) length=286 GL50803_14845 33 kda 100% 2 Hypothetical protein location=glchr01:62464 63300(+) length=278 GL50803_3491 32 kda 100% 2 Hypothetical protein location=glchr04:1295467 1296060( ) length=197 GL50803_32838 22 kda 100% 2 [2Fe 2S] ferredoxin location=glchr02:252344 252780( ) length=133 GL50803_27266 15 kda 100% 2 Hypothetical protein location=glchr03:899449 899826( ) length=125 GL50803_27910 14 kda 100% 2 Hypothetical protein location=glchr01:196648 197733(+) length=361 GL50803_3582 40 kda 100% 96% 2 1 HesB domain containing protein location=glchr01:407365 407760( ) length=131 GL50803_14821 14 kda 100% 1 Beta adaptin location=glchr05:4202692 4206090( ) length=1132 GL50803_21423 123 kda 100% 1 Hypothetical protein location=ch991767:448255 451542(+) length=1095 GL50803_16985 123 kda 100% 1 Gamma giardin location=glchr03:483217 484152( ) length=311 GL50803_17230 36 kda 100% 1 Axonemal p66.0 location=glchr03:351832 353490(+) length=552 GL50803_114462 63 kda 100% 1 Hypothetical protein location=glchr01:187549 189786(+) length=745 GL50803_13282 83 kda 100% 1 Hypothetical protein location=glchr01:187549 189786(+) length=745 GL50803_13282 83 kda 100% 1 Hypothetical protein location=glchr02:543824 545218(+) length=464 GL50803_14999 50 kda 100% 1 Kinase, NEK location=glchr04:2531182 2533878( ) length=898 GL50803_92498 102 kda 100% 1 Hypothetical protein location=glchr05:4046528 4047322( ) length=264 GL50803_4590 31 kda 99% 1 Hypothetical protein location=glchr03:1354207 1356216( ) length=669 GL50803_17312 73 kda 99% 1 Hypothetical protein location=glchr02:475201 475887(+) length=228 GL50803_10808 25 kda 99% 1 Hypothetical protein location=glchr04:435707 437125(+) length=472 GL50803_22291 52 kda 98% 1 Hypothetical protein location=glchr05:4304006 4306423(+) length=805 GL50803_8557 90 kda 95% 1 Protein required for cell viability location=glchr05:1543427 1543864(+) length=145 GL50803_8819 16 kda 100% 7 Coiled coil protein location=glchr04:330287 335314( ) length=1675 GL50803_17249 191 kda 100% 7 ELKS location=glchr01:945014 947788(+) length=924 GL50803_113603 103 kda 100% 100% 1 4 Nuclear ATP/GTP binding protein location=glchr02:315994 320262( ) length=1422 GL50803_10976 160 kda 100% 3 MDR type permease location=glchr05:895915 897915( ) length=666 GL50803_8444 74 kda 100% 3 Hypothetical protein location=glchr05:955060 959475(+) length=1471 GL50803_94542 162 kda 100% 100% 1 2 Hypothetical protein location=glchr03:1262924 1265032(+) length=702 GL50803_7723 81 kda 100% 2 Hypothetical protein location=glchr02:117748 118506( ) length=252 GL50803_16424 30 kda 100% 2 Hypothetical protein location=glchr05:3785915 3788233( ) length=772 GL50803_16648 88 kda 100% 2 Hypothetical protein location=glchr02:625502 632380(+) length=2292 GL50803_16273 253 kda 100% 1 hypothetical protein location=glchr05:1866649 1867416( ) length=255 GL50803_3141 29 kda 96% 1 Kinase, NEK location=glchr04:11022 13493( ) length=823 GL50803_16824 91 kda 100% 1 Potassium transporting ATPase alpha chain 1 location=glchr02:1325350 1329357(+) GL50803_96670 148 kda 100% 1 Rac/Rho like protein location=glchr03:1715915 1716571(+) length=218 GL50803_8496 24 kda 96% 1 Hypothetical protein location=glchr01:768208 768807(+) length=199 GL50803_7825 23 kda 96% 1 Hypothetical protein location=glchr04:577446 581231( ) length=1261 GL50803_6464 142 kda 96% 1 Protein 21.1 location=glchr05:1404750 1405742( ) length=330 GL50803_9030 37 kda 100% 95% 2 1 Coiled coil protein location=glchr02:1450392 1457219(+) length=2275 GL50803_113677 255 kda 100% 99% 1 1 Kinase, NEK location=glchr03:1127120 1127983( ) length=287 GL50803_14742 33 kda 96% 1 DNA directed RNA polymerase RPB3 location=glchr05:2530086 2531066(+) GL50803_7474 36 kda 96% 1 Manganese dependent inorganic pyrophosphatase, putative GL50803_8074 76 kda 98% 1 Vacuolar ATP synthase subunit H location=glchr01:976410 978002(+) length=530 GL50803_14961 59 kda 100% 1 Centromere/microtubule binding protein CBF5 location=glchr05:834235 835500(+) GL50803_16311 47 kda 99% 1 H SHIPPO 1 location=glchr05:4188401 4190248( ) length=615 GL50803_91354 63 kda 99% 1 Alpha 11 giardin location=glchr05:1207222 1208145( ) length=307 GL50803_17153 35 kda 96% 1

Sec23 location=glchr02:1256237 1258822(+) length=861 GL50803_9376 95 kda 96% 1 Kinase, CMGC CDK location=glchr02:1236914 1237936(+) length=340 GL50803_15397 38 kda 96% 1 Hypothetical protein location=glchr05:3774933 3777857( ) length=974 GL50803_6735 107 kda 95% 1 Nucleolar GTP binding protein 1, putative location=glchr04:1478696 1480726( ) GL50803_16371 78 kda 99% 1 Phosphatase location=glchr01:1234177 1235109( ) length=310 GL50803_15215 35 kda 100% 1 Kinase, CAMK CAMKL location=glchr03:195626 196924( ) length=432 GL50803_16034 49 kda 95% 96% 1 1 UTP glucose 1 phosphate uridylyltransferase location=glchr04:1237229 1238581(+) GL50803_29307 49 kda 100% 1 MCT 1 protein like protein location=glchr05:3812658 3813371(+) length=237 GL50803_13897 26 kda 99% 1 Kinase, NEK location=glchr05:3045352 3046401( ) length=349 GL50803_6680 39 kda 96% 1 Nucleoside diphosphate kinase location=glchr03:462824 463987( ) length=387 GL50803_14135 43 kda 96% 1 Hypothetical protein location=glchr05:1356529 1357125( ) length=198 GL50803_8657 22 kda 96% 1 Phosphatase location=glchr02:159399 160556( ) length=385 GL50803_14404 42 kda 96% 1 Transglutaminase/protease, putative location=glchr05:3836233 3837606( ) GL50803_16817 52 kda 96% 1 Phosphatidate cytidylyltransferase location=glchr03:241108 242238( ) length=376 GL50803_16906 42 kda 95% 1 Phosphorylated CTD interacting factor PCIF1 location=glchr04:1838064 1839863( ) GL50803_24111 69 kda 96% 1 IFT complex B location=glchr03:947370 949388( ) length=672 GL50803_15428 76 kda 96% 1 Hypothetical protein location=glchr04:218879 220696( ) length=605 GL50803_17474 68 kda 99% 1 Clathrin heavy chain location=glchr04:1021986 1027601(+) length=1871 GL50803_102108 207 kda 100% 1 Hypothetical protein location=glchr03:1585416 1587962( ) length=848 GL50803_103709 95 kda 96% 1 Spindle pole protein, putative location=glchr05:1004642 1006306( ) length=554 GL50803_17055 62 kda 96% 1 L asparaginase location=glchr04:1495446 1496648(+) length=400 GL50803_7865 44 kda 96% 1 Kinase, NEK location=glchr04:2455966 2457126(+) length=386 GL50803_13921 43 kda 96% 1 Translation initiation factor 2 gamma subunit location=glchr04:1777620 1779056(+) GL50803_2970 52 kda 96% 1 Translation initiation factor eif 4A, putative location=glchr01:1336531 1337706( ) GL50803_10255 43 kda 96% 95% 1 1 Protein 21.1 1 location=glchr05:2658864 2661110(+) length=748 GL50803_11165 82 kda 99% 100% 100% 1 2 1 Ser/Thr phosphatase 2C, putative location=glchr02:1114771 1115853( ) GL50803_11740 39 kda 96% 1 La ribonucleoprotein, putative location=glchr05:1704672 1705718( ) length=348 GL50803_9803 39 kda 100% 1 Protein 21.1 location=glchr03:1760044 1764417(+) length=1457 GL50803_17097 163 kda 100% 100% 7 1 Trichohyalin location=glchr05:4273851 4278095( ) length=1414 GL50803_32375 156 kda 100% 1 Kinase, NEK location=glchr03:476501 478453(+) length=650 GL50803_113553 72 kda 100% 100% 1 1 Protein 21.1 location=glchr04:572878 577380( ) length=1500 GL50803_17130 164 kda 100% 99% 6 1 Hypothetical protein location=glchr05:1583174 1584202( ) length=342 GL50803_16550 37 kda 99% 100% 95% 1 4 1 Alcohol dehydrogenase lateral transfer candidate location=glchr04:2352037 GL50803_13350 49 kda 96% 1 Beta giardin location=glchr04:2015776 2016594(+) length=272 GL50803_4812 31 kda 100% 95% 2 1 Hypothetical protein location=glchr02:249807 250571(+) length=254 GL50803_17037 29 kda 100% 97% 1 1 26S proteasome regulatory subunit, putative location=glchr01:1198367 1202413(+) GL50803_91643 148 kda 96% 1 Type II inositol 1,4,5 trisphosphate 5 phosphatase precursor GL50803_14787 81 kda 100% 1 Hypothetical protein location=glchr02:139251 140732( ) length=493 GL50803_11380 52 kda 96% 1 Uridine kinase location=glchr05:1578937 1580985( ) length=682 GL50803_16549 77 kda 96% 1 Hypothetical protein location=glchr04:412122 413564(+) length=480 GL50803_94463 53 kda 96% 1 hypothetical protein location=glchr04:461008 463569(+) length=853 GL50803_14509 94 kda 96% 1 Hypothetical protein location=glchr04:1946060 1946986( ) length=308 GL50803_11604 35 kda 95% 1 Uridine kinase location=glchr03:424334 426082(+) length=582 GL50803_8217 66 kda 96% 1 Kinase, NEK location=glchr05:3818135 3819616( ) length=493 GL50803_5346 55 kda 100% 2 Long chain fatty acid CoA ligase 5 location=glchr05:3954142 3956439(+) GL50803_21118 85 kda 100% 2 Histone H2B location=glchr05:3406202 3406594(+) length=130 GL50803_121045 15 kda 95% 1 Kinase, CMGC DYRK location=glchr01:164184 166766( ) length=860 GL50803_137695 97 kda 96% 1 DNA topoisomerase III location=glchr04:1111493 1114414(+) length=973 GL50803_15190 107 kda 96% 1 Phosphoenolpyruvate carboxykinase location=glchr03:850721 852685( ) GL50803_10623 74 kda 100% 3 Hypothetical protein location=glchr04:1834261 1834638(+) length=125 GL50803_6812 14 kda 95% 1 Peptide methionine sulfoxide reductase msrb location=glchr05:3407196 3407672(+) GL50803_5180 17 kda 95% 1 Dynein intermediate chain location=glchr02:797338 799611(+) length=757 GL50803_33218 84 kda 100% 1 CAMP dependent protein kinase regulatory chain location=glchr05:2786128 GL50803_9117 51 kda 100% 3 Ribosome biogenesis protein BMS1 location=glchr03:1319716 1323597(+) GL50803_102722 146 kda 100% 1 hypothetical protein location=glchr04:2113758 2116376( ) length=872 GL50803_12207 95 kda 100% 100% 99% 95% 1 3 1 1

Histidyl trna synthetase location=glchr01:1398857 1401742( ) length=961 GL50803_22808 105 kda 96% 1 70 kda peptidylprolyl isomerase, putative location=glchr04:335471 336487( ) GL50803_3643 38 kda 100% 2 Nitroreductase Fd NR2 location=glchr01:373022 373816(+) length=264 GL50803_22677 29 kda 96% 1 Hypothetical protein location=glchr02:208489 209448( ) length=319 GL50803_101918 37 kda 99% 1 Aminoacyl histidine dipeptidase location=glchr05:1333710 1335230(+) length=506 GL50803_8407 55 kda 100% 1 Kinase, STE STE20 location=ch991761:251395 252666(+) length=423 GL50803_10609 47 kda 96% 1 Farnesyl diphosphate synthase location=glchr04:541501 542724( ) length=407 GL50803_6633 46 kda 96% 1 Protein 21.1 location=glchr05:4353561 4355606( ) length=681 GL50803_12139 76 kda 100% 2 A type flavoprotein lateral transfer candidate location=glchr04:1730357 1731601( ) GL50803_10358 47 kda 100% 2 Protein 21.1 location=glchr03:1757632 1759914( ) length=760 GL50803_17096 85 kda 100% 100% 100% 1 2 2 Protein phosphatase 2A regulatory subunit, putative location=glchr04:1618007 GL50803_9894 101 kda 100% 2 Rab2a location=glchr04:106850 107494( ) length=214 GL50803_15567 24 kda 100% 100% 1 1 Hypothetical protein location=glchr02:1148294 1149589( ) length=431 GL50803_15499 49 kda 100% 99% 1 1 Kinesin 13 location=glchr03:997931 1000075( ) length=714 GL50803_16945 80 kda 100% 1 Hypothetical protein location=glchr05:2019199 2020965(+) length=588 GL50803_11105 65 kda 100% 100% 1 1 Kinase, NEK frag location=glchr02:123292 125412(+) length=706 GL50803_15411 78 kda 100% 2 hypothetical protein location=glchr05:4024720 4029309( ) length=1529 GL50803_14390 173 kda 100% 1 Kinase, CAMK CAMKL location=glchr05:4081287 4082498(+) length=403 GL50803_16235 46 kda 96% 1 Phosphorylase B kinase gamma catalytic chain location=glchr04:1789017 1791329( ) GL50803_10698 85 kda 96% 1 Hypothetical protein location=glchr04:481202 483829(+) length=875 GL50803_16507 99 kda 95% 1 Protein 21.1 location=glchr04:2128398 2130755( ) length=785 GL50803_4383 84 kda 95% 1 Ribosomal protein S29A location=glchr05:2867879 2868292(+) length=137 GL50803_39483 15 kda 100% 1 TCP 1 chaperonin subunit alpha location=glchr04:1267312 1268964(+) length=550 GL50803_91919 59 kda 99% 1 Coiled coil protein location=ch991767:452286 457343(+) length=1685 GL50803_15591 186 kda 100% 2 TCP 1 chaperonin subunit beta location=glchr02:118733 120310( ) length=525 GL50803_11397 57 kda 100% 2 Hypothetical protein location=glchr01:622857 624443(+) length=528 GL50803_8528 57 kda 100% 1 Protein F10G7.1 location=glchr04:2240683 2243163(+) length=826 GL50803_5294 93 kda 100% 2 Dual specificity phosphatase Cdc25, putative location=glchr03:1385171 1385533(+) GL50803_4369 13 kda 95% 100% 1 1 Dipeptidyl peptidase III location=glchr01:170598 172682(+) length=694 GL50803_3822 78 kda 100% 1 Hypothetical protein location=ch991767:1115776 1119564( ) length=1262 GL50803_95787 147 kda 96% 1 Prefoldin subunit 3, putative location=glchr01:311816 312394(+) length=192 GL50803_7030 22 kda 100% 1 Nucleolar protein NOP5 location=glchr05:2490068 2491909(+) length=613 GL50803_5359 69 kda 100% 100% 100% 1 1 2 Ubiquitin conjugating enzyme E1 location=glchr05:2463417 2466695( ) GL50803_10661 121 kda 100% 2 DEAD box RNA helicase Vasa location=glchr05:1279223 1280572(+) length=449 GL50803_34684 50 kda 100% 1 TCP 1 chaperonin subunit theta location=glchr02:523561 525252( ) length=563 GL50803_13500 61 kda 100% 1 Alcohol dehydrogenase lateral transfer candidate location=glchr03:1728021 GL50803_3593 45 kda 100% 1 Ribosomal protein L7 location=glchr04:2481439 2482146(+) length=235 GL50803_19436 27 kda 100% 100% 1 1 Kinase, NEK location=glchr05:2956107 2956973( ) length=288 GL50803_24321 33 kda 100% 3 Hypothetical protein location=glchr05:3737483 3738271( ) length=262 GL50803_16717 29 kda 100% 95% 3 1 Proteasome subunit beta type 1 location=glchr05:2125550 2126314( ) length=254 GL50803_13127 28 kda 100% 2 Nucleolar protein NOP5 location=glchr01:745277 746773( ) length=498 GL50803_10577 55 kda 100% 2 Coiled coil protein location=glchr05:3547580 3552538( ) length=1652 GL50803_16342 186 kda 100% 1 Metalloprotease, insulinase family location=glchr02:1405802 1409320(+) GL50803_9508 131 kda 100% 2 Hypothetical protein location=glchr02:1086416 1087129( ) length=237 GL50803_8002 27 kda 100% 1 Translation elongation factor location=glchr01:1067637 1068299( ) length=220 GL50803_13561 25 kda 100% 2 Hypothetical protein location=glchr05:4283113 4283865( ) length=250 GL50803_10524 27 kda 100% 2 Hypothetical protein location=glchr03:469439 470080(+) length=213 GL50803_6185 25 kda 100% 1 hypothetical protein location=glchr03:584909 597991( ) length=4360 GL50803_113592 489 kda 96% 1 20S proteasome alpha subunit 5 location=glchr02:799696 800433( ) length=245 GL50803_2980 27 kda 100% 2 Serine/threonine protein phosphatase PP X isozyme 2 location=glchr02:908276 GL50803_14311 38 kda 100% 1 Ubiquitin carboxyl terminal hydrolase 4 location=glchr05:2525772 2528231( ) GL50803_14460 93 kda 100% 2 Hypothetical protein location=glchr03:1813097 1813711( ) length=204 GL50803_10868 23 kda 100% 1 Kinase, NEK location=glchr04:1908314 1910068( ) length=584 GL50803_9421 64 kda 100% 2 Nucleolar GTP binding protein 2 location=glchr05:2003305 2005518( ) length=737 GL50803_89887 84 kda 100% 2 Hypothetical protein location=glchr03:1019029 1020021(+) length=330 GL50803_10316 37 kda 100% 100% 1 1

Glutaminyl trna synthetase location=glchr05:3469699 3471807( ) length=702 GL50803_86681 80 kda 100% 1 Rab11 location=glchr05:2730259 2730909( ) length=216 GL50803_1695 24 kda 96% 100% 1 3 Alanine aminotransferase, putative location=glchr03:667326 668891(+) length=521 GL50803_12150 58 kda 100% 3 Hypothetical protein location=glchr04:2282398 2284935( ) length=845 GL50803_27602 96 kda 100% 2 Prolyl trna synthetase location=glchr04:2274485 2276113(+) length=542 GL50803_15983 61 kda 100% 1 Hypothetical protein location=glchr05:4333683 4336562(+) length=959 GL50803_102022 107 kda 99% 100% 1 1 TBP interacting protein TIP49 location=glchr02:679775 681181(+) length=468 GL50803_9825 51 kda 100% 3 Hypothetical protein location=glchr05:951110 954820(+) length=1236 GL50803_16365 136 kda 100% 100% 99% 2 2 Aldose reductase location=glchr02:157396 158337(+) length=313 GL50803_7260 35 kda 100% 2 Hypothetical protein location=glchr02:890655 891470(+) length=271 GL50803_6330 29 kda 100% 2 Serine/threonine protein phosphatase 7 location=glchr05:1221649 1222905(+) GL50803_7588 47 kda 100% 2 Hypothetical protein location=glchr05:2638973 2639803(+) length=276 GL50803_17062 31 kda 100% 100% 100% 2 1 2 hypothetical protein location=glchr02:547395 548666( ) length=423 GL50803_9612 47 kda 100% 2 Kinase, NEK location=glchr02:1091453 1092310(+) length=285 GL50803_101534 31 kda 100% 2 Hypothetical protein location=glchr05:1210189 1212855(+) length=888 GL50803_5648 100 kda 100% 100% 100% 2 3 2 Dual specificity protein phosphatase CDC14A location=glchr04:276551 278383(+) GL50803_9270 66 kda 100% 99% 1 1 Hypothetical protein location=glchr04:1327288 1328670(+) length=460 GL50803_8883 52 kda 96% 100% 1 2 Hypothetical protein location=glchr05:2260480 2260851( ) length=123 GL50803_3910 14 kda 100% 1 Hypothetical protein location=glchr02:1131140 1131922(+) length=260 GL50803_11755 30 kda 100% 3 Fibrillarin like pre rrna processing protein Narcisi et al location=glchr05:4262865 GL50803_97219 35 kda 100% 3 hypothetical protein location=glchr04:878097 881987(+) length=1296 GL50803_11932 145 kda 100% 3 Kinase, CMGC MAPK location=glchr05:1311119 1312201( ) length=360 GL50803_22850 41 kda 100% 3 TCP 1 chaperonin subunit eta location=glchr05:2528272 2530059( ) length=595 GL50803_16124 65 kda 100% 1 S adenosylmethionine synthetase location=glchr01:1096377 1097687(+) GL50803_5659 49 kda 100% 2 Protein 21.1 1 location=glchr05:1007720 1010854( ) length=1044 GL50803_17053 114 kda 100% 100% 4 1 Kinase, NEK location=glchr03:1279611 1280741(+) length=376 GL50803_95593 42 kda 100% 100% 1 1 Kinase, NEK location=glchr04:1300517 1301734(+) length=405 GL50803_5375 45 kda 100% 1 Threonyl trna synthetase location=glchr05:1205079 1207166(+) length=695 GL50803_13998 79 kda 100% 3 Kinesin 14 location=glchr01:89419 92937( ) length=1172 GL50803_13797 131 kda 100% 5 hypothetical protein location=glchr04:530819 533830( ) length=1003 GL50803_96324 111 kda 100% 100% 100% 1 1 4 Bip location=glchr04:535188 537221(+) length=677 GL50803_17121 74 kda 100% 100% 100% 1 3 3 Hypothetical protein location=glchr05:3484925 3490639(+) length=1904 GL50803_9183 214 kda 100% 3 Ribosomal protein S7 location=glchr05:3929431 3930003( ) length=190 GL50803_14329 22 kda 100% 2 Coiled coil protein location=ch991776:375101 378361(+) length=1086 GL50803_16152 121 kda 100% 5 Plasma membrane calcium transporting ATPase 2 location=glchr05:4209683 GL50803_32658 120 kda 100% 100% 3 5 NADH oxidase lateral transfer candidate location=glchr01:307195 308649( ) GL50803_33769 53 kda 100% 100% 1 3 Hypothetical protein location=glchr04:529114 530817(+) length=567 GL50803_96306 63 kda 100% 4 Phosphomannomutase 2 location=glchr04:363536 365503( ) length=655 GL50803_17254 74 kda 100% 3 FKBP type peptidyl prolyl cis trans isomerase location=glchr01:755256 756320( ) GL50803_10570 39 kda 100% 3 Alcohol dehydrogenase 3 lateral transfer candidate location=glchr03:197300 GL50803_3861 45 kda 100% 1 Hexose transporter location=glchr03:949443 950981( ) length=512 GL50803_11540 56 kda 95% 95% 1 1 Protein 21.1 location=glchr04:455157 457328(+) length=723 GL50803_7679 78 kda 95% 1 Hypothetical protein location=glchr01:242803 243576( ) length=257 GL50803_8627 27 kda 95% 96% 96% 97% 1 1 1 1 Glucokinase location=glchr05:1550601 1551632(+) length=343 GL50803_8826 38 kda 100% 2 U3 small nucleolar RNA associated protein 11, putative location=glchr04:1839951 GL50803_1894 26 kda 96% 1 Kinase, NEK location=glchr03:1829978 1832179( ) length=733 GL50803_9327 82 kda 96% 1 Cysteinyl trna synthetase location=glchr04:1397773 1399710( ) length=645 GL50803_5867 73 kda 96% 1 Vacuolar ATP synthase subunit C location=glchr02:393222 394706(+) length=494 GL50803_87058 56 kda 96% 1 Phosphoglycolate phosphatase location=glchr05:3984009 3984695(+) length=228 GL50803_14546 25 kda 95% 1 Ubiquitin conjugating enzyme E2 17 kda location=glchr02:1023303 1023779(+) GL50803_3978 18 kda 95% 1 Hypothetical protein location=glchr04:1818916 1819341( ) length=141 GL50803_14299 16 kda 95% 95% 1 1 FKBP type peptidyl prolyl cis trans isomerase location=glchr01:1233776 1234105( ) GL50803_10450 12 kda 100% 1 Hypothetical protein location=glchr02:1095473 1095718(+) length=81 GL50803_8394 9 kda 95% 1 GTP binding protein location=glchr05:1234471 1235994( ) length=507 GL50803_17149 57 kda 100% 1 Hypothetical protein location=glchr05:1336634 1337971(+) length=445 GL50803_8405 48 kda 99% 1

Spindle pole protein, putative location=glchr03:723448 725529( ) length=693 GL50803_14181 76 kda 99% 1 Hypothetical protein location=glchr02:576372 576833(+) length=153 GL50803_5568 17 kda 100% 1 Arsenical pump driving ATPase location=glchr03:1410522 1411586( ) length=354 GL50803_7953 40 kda 96% 1 Hypothetical protein location=glchr05:4284903 4285757(+) length=284 GL50803_10522 30 kda 98% 96% 95% 100% 1 1 1 1 Hypothetical protein location=glchr01:1150171 1150593( ) length=140 GL50803_2012 16 kda 100% 1 Alanyl trna synthetase location=glchr04:1566990 1569899( ) length=969 GL50803_96460 108 kda 100% 1 Protein 21.1 location=glchr05:2201241 2204018(+) length=925 GL50803_14859 103 kda 99% 1 L serine dehydratase location=glchr03:1193311 1194861(+) length=516 GL50803_15090 56 kda 96% 1 TBP interacting protein TIP49 location=glchr04:2689436 2690887( ) length=483 GL50803_17565 53 kda 98% 1 Protein 21.1 location=glchr05:1966122 1971743( ) length=1873 GL50803_103810 206 kda 100% 1 LEK1 location=glchr05:1584394 1586544(+) length=716 GL50803_86761 79 kda 100% 1 Hypothetical protein location=glchr05:3559348 3560799( ) length=483 GL50803_13075 54 kda 96% 1 Vacuolar protein sorting 26, putative location=glchr05:1425877 1427472(+) GL50803_100864 57 kda 100% 1 Ubiquitin fusion degradation protein 1 location=glchr01:687262 688203( ) GL50803_3994 35 kda 96% 1 Spindle pole protein, putative location=glchr05:1543866 1546964( ) length=1032 GL50803_8820 113 kda 100% 1 organism=crap product=crap_aldoa_rabit GL50803_20130614107 39 kda 95% 1 Hypothetical protein location=glchr01:947822 949909( ) length=695 GL50803_13651 78 kda 100% 96% 100% 3 1 1 Hypothetical protein location=glchr03:1261045 1262844(+) length=599 GL50803_16596 67 kda 95% 1 Ripening regulated protein DDTFR19 location=glchr05:1455859 1456047(+) GL50803_14457 7 kda 98% 100% 1 1 SnRNP Sm like protein, putative location=glchr04:1674717 1675094(+) length=125 GL50803_6408 14 kda 100% 1 Hypothetical protein location=glchr03:1173065 1173736( ) length=223 GL50803_11034 25 kda 100% 1 Protein 21.1 location=glchr05:2007327 2009987( ) length=886 GL50803_11099 98 kda 100% 1 Hypothetical protein location=glchr05:3813460 3816432(+) length=990 GL50803_16653 103 kda 100% 1 Hypothetical protein location=glchr05:2951652 2954912( ) length=1086 GL50803_4266 121 kda 100% 2 Polyadenylate binding protein, putative location=glchr01:642027 643355( ) GL50803_59425942 50 kda 100% 1 ATP dependent RNA helicase location=glchr05:3177173 3179023( ) length=616 GL50803_96537 69 kda 100% 2 ATP dependent RNA helicase p47, putative location=glchr05:1276972 1278522(+) GL50803_16376 57 kda 100% 2 ACTIN location=glchr02:1296903 1298030( ) length=375 GL50803_40817 42 kda 99% 100% 95% 1 2 1 Ribosomal protein L7Ae location=glchr04:1207642 1208010( ) length=122 GL50803_2825 13 kda 100% 1 ARF GAP location=glchr05:982690 983886( ) length=398 GL50803_2834 42 kda Hypothetical protein location=glchr04:1565960 1566955(+) length=331 GL50803_8692 37 kda 100% 2 Ribose 5 phosphate isomerase location=glchr03:1049016 1049453( ) length=145 GL50803_27614 15 kda 100% 1 Eukaryotic translation initiation factor 2 beta subunit location=glchr05:1410138 GL50803_91398 37 kda 100% 2 Hypothetical protein location=glchr03:725577 726404(+) length=275 GL50803_6367 31 kda 95% 100% 95% 1 2 1 NADH oxidase location=glchr05:2174947 2176236(+) length=429 GL50803_9719 47 kda 100% 1 Low molecular weight protein tyrosine phosphatase location=glchr05:1455282 GL50803_14456 18 kda 100% 2 NADPH oxidoreductase, putative location=glchr05:1217835 1218329( ) length=164 GL50803_17150 19 kda 100% 1 Hypothetical protein location=glchr03:1304759 1305172( ) length=137 GL50803_6752 15 kda 100% 95% 1 1 Ribosomal protein L5 location=glchr02:804131 805024(+) length=297 GL50803_17395 34 kda 100% 2 Ribosomal protein S27 location=glchr04:1245273 1245647(+) length=124 GL50803_10780 14 kda 100% 2 Cathepsin B precursor location=glchr04:2314738 2315640( ) length=300 GL50803_14019 33 kda 95% 97% 1 1 Translation initiation inhibitor location=glchr02:137274 137636(+) length=120 GL50803_480 13 kda 100% 2 Ser/Thr protein kinase location=glchr04:733322 735931( ) length=869 GL50803_22003 94 kda 100% 100% Zinc finger domain location=glchr02:399455 399841(+) length=128 GL50803_16877 15 kda 100% 100% 1 1 Hypothetical protein location=glchr05:3238894 3240900(+) length=668 GL50803_5822 74 kda 100% 1 Hypothetical protein location=glchr01:1194573 1195151(+) length=192 GL50803_7244 21 kda 100% 1 RNA binding protein, putative location=glchr05:2818404 2819225(+) length=273 GL50803_11186 32 kda 100% 2 Heat shock protein, putative location=glchr04:1692786 1695323(+) length=845 GL50803_16412 95 kda 100% 3 Hypothetical protein location=glchr05:1869412 1870278( ) length=288 GL50803_13103 33 kda 100% 2 Hypothetical protein location=glchr03:490036 491157( ) length=373 GL50803_6120 42 kda 100% 2 Cluster of SKD1 protein location=glchr05:3100075 3101439(+) length=454 GL50803_101906 [2] 50 kda 100% 2 Topoisomerase II location=glchr05:2989657 2991216(+) length=519 GL50803_16795 57 kda 100% 2 Threonine dehydratase lateral transfer candidate location=glchr04:52029 54842( ) GL50803_12108 104 kda 100% 1 CGI 35 protein, putative location=glchr01:1219297 1219869(+) length=190 GL50803_10459 22 kda 100% 1 Kinesin 3 location=glchr04:998288 1001368(+) length=1026 GL50803_102101 115 kda 90% 100% 1 2

hypothetical protein location=glchr03:858974 859408(+) length=144 GL50803_6340 17 kda 100% 2 Kinesin like protein location=glchr01:816079 818469( ) length=796 GL50803_17264 88 kda 100% 1 Hypothetical protein location=glchr04:2092394 2092927( ) length=177 GL50803_15600 19 kda 100% 1 Phosphatidylinositol 4 phosphate 5 kinase, putative location=glchr02:156094 GL50803_7261 43 kda 100% 100% 6 1 Branched chain amino acid aminotransferase lateral transfer candidate GL50803_6184 39 kda 100% 100% 1 5 Hypothetical protein location=glchr04:2481039 2481491( ) length=150 GL50803_16522 17 kda 100% 1 Ribosomal protein L30 location=glchr05:2161528 2161857(+) length=109 GL50803_14321 12 kda 100% 2 WD 40 repeat protein family location=glchr01:1299082 1300110( ) length=342 GL50803_10822 39 kda 96% 100% 1 2 Ribosomal protein L15 location=glchr02:1087216 1087830(+) length=204 GL50803_8001 24 kda 100% 100% 100% 100% 100% 2 2 1 1 1 Eukaryotic translation initiation factor 5A location=glchr05:829656 830105(+) GL50803_14614 17 kda 100% 1 Heat shock protein HSP 90 alpha location=glchr05:1731251 1732225(+) length=324 GL50803_13864 37 kda 100% 3 Ribosomal protein L44 location=glchr05:1714863 1715183( ) length=106 GL50803_9810 12 kda 100% 100% 96% 100% 100% 1 1 1 1 1 Ribosomal protein L7a location=glchr03:579698 580484(+) length=225 GL50803_17244 25 kda 99% 95% 100% 100% 95% 1 1 1 3 1 Hypothetical protein location=glchr01:1270105 1273020( ) length=971 GL50803_90418 110 kda 100% 100% 1 1 Pyrophosphate fructose 6 phosphate 1 phosphotransferase alpha subunit GL50803_14993 60 kda 100% 2 Hypothetical protein location=glchr02:1118864 1120303(+) length=479 GL50803_11743 54 kda 100% 100% 2 1 Hypothetical protein location=glchr04:1805318 1807360( ) length=680 GL50803_10708 75 kda 100% 3 Hypothetical protein location=glchr02:1248127 1248393( ) length=88 GL50803_13268 10 kda 100% 2 Liver stage antigen like protein location=glchr03:1265109 1270544(+) length=1811 GL50803_16595 201 kda 100% 100% 100% 100% 4 12 1 4 Ribosomal protein L9 location=glchr05:1003973 1004530( ) length=185 GL50803_17056 21 kda 100% 100% 100% 1 2 2 Aminoacyl histidine dipeptidase location=glchr05:3627220 3628794(+) length=524 GL50803_15832 57 kda 100% 8 Glutamate synthase location=glchr05:4017659 4020391( ) length=910 GL50803_7195 98 kda 100% 6 Ribosomal protein L13a location=glchr04:2660666 2661259(+) length=197 GL50803_11247 23 kda 95% 100% 92% 1 1 0 Ribosomal protein L32 location=glchr04:1407911 1408321(+) length=136 GL50803_14049 16 kda 100% 2 Heat shock protein HSP 90 alpha location=glchr05:2514317 2515447(+) length=376 GL50803_98054 43 kda 100% 4 Kinase, STE STE20 location=glchr03:1114007 1115590( ) length=527 GL50803_15514 58 kda 100% 3 Protein 21.1 location=glchr04:312948 313979( ) length=343 GL50803_102813 37 kda 100% 4 Chaperone protein DnaJ location=glchr05:1713531 1714760( ) length=409 GL50803_9808 45 kda 100% 4 Hypothetical protein location=glchr05:4077692 4078795(+) length=367 GL50803_13272 40 kda 100% 1 Transketolase location=glchr03:349269 351428( ) length=719 GL50803_9704 78 kda 100% 3 Glucosamine 6 phosphate N acetyltransferase location=glchr05:2046997 2047443( ) GL50803_14651 17 kda 100% 2 Deoxyribose phosphate aldolase lateral transfer candidate location=glchr04:1847981 GL50803_15127 27 kda 96% 1 Dynamin location=glchr03:741223 743421(+) length=732 GL50803_14373 80 kda 100% 100% 100% 100% 100% 4 1 1 1 1 Hypothetical protein location=glchr04:596559 596792(+) length=77 GL50803_34093 9 kda 100% 100% 100% 1 1 3 4 methyl 5 thiazole monophosphate biosynthesis enzyme location=glchr02:972691 GL50803_9088 20 kda 100% 2 Glycogen phosphorylase location=glchr05:3185783 3188557(+) length=924 GL50803_6226 105 kda 100% 3 Ribokinase location=glchr01:1309908 1310942(+) length=344 GL50803_15297 37 kda 100% 2 Ser/Thr protein kinase location=glchr02:125385 126242(+) length=285 GL50803_15410 32 kda 100% 100% 1 2 Protein 21.1 location=glchr05:2791061 2794816(+) length=1251 GL50803_14872 137 kda 100% 100% 2 1 Hypothetical protein location=glchr02:1019733 1022081(+) length=782 GL50803_16602 86 kda 100% 2 Hypothetical protein location=glchr03:1245244 1248321( ) length=1025 GL50803_42357 115 kda 100% 100% 100% 4 2 1 FixW protein, putative location=glchr05:3843149 3843550( ) length=133 GL50803_6289 15 kda 100% 2 Adenylate kinase location=glchr01:1293692 1294264( ) length=190 GL50803_90402 21 kda 100% 2 Giardia trophozoite antigen GTA 2 location=glchr03:946579 947289( ) length=236 GL50803_15427 27 kda 100% 96% 99% 2 1 1 Ribosomal protein L37 location=glchr02:895325 895594( ) length=89 GL50803_14171 10 kda 100% 100% 100% 100% 100% 3 2 1 1 2 Dynein light chain location=glchr05:2585684 2586118( ) length=144 GL50803_14270 16 kda 100% 2 Hypothetical protein location=glchr03:1115627 1119940( ) length=1437 GL50803_11877 155 kda 100% 2 Hypothetical protein location=glchr04:1646820 1647215( ) length=131 GL50803_5810 15 kda 100% 100% 1 1 Ubiquitin conjugating enzyme E2 17 kda 3 location=ch991761:255420 255956(+) GL50803_15252 20 kda 100% 1 Hypothetical protein, similar to AMMECR1 location=glchr05:3956710 3957303( ) GL50803_9349 22 kda 100% 1 Ribosomal protein L36 1 location=glchr05:1357236 1357508(+) length=90 GL50803_16114 11 kda 100% 100% 1 2 Ribosomal protein L35 location=glchr05:4085972 4086430( ) length=152 GL50803_6133 17 kda 100% 100% 100% 100% 100% 1 1 3 1 2 Hypothetical protein location=glchr01:30297 33218( ) length=973 GL50803_94534 108 kda 100% 100% 100% 100% 1 4 1 10 Hypothetical protein location=glchr05:4245511 4249503(+) length=1330 GL50803_13771 147 kda 100% 100% 100% 3 2 3 Hypothetical protein location=glchr01:1217511 1219220(+) length=569 GL50803_10460 63 kda 100% 100% 100% 6 4 1

Ribosomal protein L35a location=glchr01:635668 636039(+) length=123 GL50803_5947 14 kda 96% 100% 100% 1 2 2 Hypothetical protein location=ch991767:1110208 1111368(+) length=386 GL50803_13584 44 kda 100% 100% 100% 1 2 3 Hypothetical protein location=glchr03:260572 261723( ) length=383 GL50803_21628 42 kda 100% 7 Protein 21.1 location=glchr05:2686379 2689537( ) length=1052 GL50803_10911 115 kda 100% 100% 100% 100% 1 4 2 4 Spindle pole protein, putative location=glchr04:1876707 1878578( ) length=623 GL50803_24122 75 kda 100% 100% 2 1 hypothetical protein location=glchr03:96862 98337(+) length=491 GL50803_5167 55 kda 95% 100% 1 7 Hypothetical protein location=glchr05:2799375 2800898(+) length=507 GL50803_14868 55 kda 100% 100% 100% 3 2 2 Pyruvate flavodoxin oxidoreductase location=glchr05:2633106 2636705( ) GL50803_17063 132 kda 100% 6 Hypothetical protein location=glchr04:1900251 1902551(+) length=766 GL50803_14948 83 kda 100% 100% 2 1 Protein 21.1 location=glchr05:2663387 2665258(+) length=623 GL50803_17060 66 kda 100% 100% 1 6 Ribosomal protein S15 location=glchr05:2468341 2468778( ) length=145 GL50803_15260 17 kda 100% 100% 2 1 Phosphatidylinositol transfer protein alpha isoform location=glchr02:266059 GL50803_4197 39 kda 100% 8 Ribosomal protein L11 location=glchr05:3392830 3393351( ) length=173 GL50803_5593 20 kda 95% 95% 100% 100% 97% 1 1 2 1 1 Pyruvate kinase location=glchr03:652902 654866( ) length=654 GL50803_3206 71 kda 100% 100% 1 3 Elongation factor 1 gamma location=glchr04:57528 58736( ) length=402 GL50803_12102 45 kda 98% 100% 1 4 Vacuolar ATP synthase subunit B location=glchr04:2101514 2103007(+) length=497 GL50803_12216 55 kda 100% 6 ATP dependent RNA helicase like protein location=glchr05:1698177 1700147(+) GL50803_15048 73 kda 100% 6 Vacuolar ATP synthase catalytic subunit A location=glchr03:288880 290847(+) GL50803_7532 72 kda 100% 6 Protein 21.1 location=glchr03:1523457 1526927( ) length=1156 GL50803_7268 126 kda 100% 2 Ubiquitin location=glchr05:1573552 1573800(+) length=82 GL50803_7110 9 kda 100% 100% 4 1 Hypothetical protein location=glchr01:725572 726066(+) length=164 GL50803_10196 18 kda 100% 3 Hypothetical protein location=glchr04:2001138 2003357( ) length=739 GL50803_115478 80 kda 100% 3 Ribosomal protein L24 location=glchr05:2798657 2799226(+) length=189 GL50803_14869 23 kda 100% 100% 2 1 2,3 bisphosphoglycerate independent phosphoglycerate mutase GL50803_8822 65 kda 100% 4 Dynein light chain location=glchr01:809935 810204( ) length=89 GL50803_9848 10 kda 100% 3 20S proteasome alpha subunit 4 location=glchr05:1198637 1199281(+) length=214 GL50803_15099 23 kda 100% 2 Cytidine deaminase location=glchr01:1154613 1155017(+) length=134 GL50803_2101 14 kda 100% 3 Kinase, NEK location=glchr02:127987 130320(+) length=777 GL50803_11390 85 kda 100% 4 Hypothetical protein location=glchr05:4154698 4155213(+) length=171 GL50803_16044 20 kda 100% 2 Hypothetical protein location=glchr01:1459448 1461313( ) length=621 GL50803_26364 70 kda 100% 5 Hypothetical protein location=glchr01:1056830 1057360(+) length=176 GL50803_11984 20 kda 100% 96% 2 1 GTP binding protein Sar1 location=glchr02:1175035 1175610( ) length=191 GL50803_7569 21 kda 100% 1 TCP 1 chaperonin subunit epsilon location=glchr01:1045147 1046808( ) GL50803_11992 61 kda 100% 3 Proteasome subunit beta type 5 precursor location=glchr05:1312757 1313641(+) GL50803_12949 32 kda 100% 3 Ribosomal protein S2 location=glchr05:3165605 3166333( ) length=242 GL50803_8118 27 kda 100% 100% 95% 100% 100% 97% 1 1 1 3 2 1 Ribosomal protein L24A location=glchr05:4082559 4082867(+) length=102 GL50803_19003 12 kda 100% 100% 100% 6 1 4 Hypothetical protein location=glchr01:34869 37850( ) length=993 GL50803_103082 107 kda 100% 100% 100% 3 1 8 Ribosomal protein S3a location=glchr05:1319790 1320536( ) length=248 GL50803_16265 28 kda 99% 100% 100% 100% 100% 1 1 1 4 3 Ribosomal protein S4 location=glchr04:195899 196705( ) length=268 GL50803_11359 30 kda 99% 100% 100% 100% 100% 1 2 2 3 1 Malic enzyme location=glchr05:3782272 3783945( ) length=557 GL50803_14285 62 kda 100% 100% 100% 2 5 1 Histone H2A location=glchr03:233537 233911( ) length=124 GL50803_27521 14 kda 95% 95% 100% 100% 100% 1 1 2 2 2 Hypothetical protein location=glchr04:472479 475139( ) length=886 GL50803_16504 97 kda 100% 100% 100% 100% 3 3 3 6 Median body protein location=glchr05:3552633 3555206(+) length=857 GL50803_16343 101 kda 100% 100% 3 2 AAA family ATPase location=glchr04:2615634 2618246(+) length=870 GL50803_16867 96 kda 100% 9 Alcohol dehydrogenase location=glchr03:1463926 1466592(+) length=888 GL50803_93358 97 kda 100% 7 Aspartyl trna synthetase location=glchr03:1808321 1809988( ) length=555 GL50803_10870 63 kda 100% 100% 2 5 Protein 21.1 location=glchr04:549675 552035(+) length=786 GL50803_27925 88 kda 100% 100% 100% 4 1 1 Ribosomal protein L12 location=glchr02:1255610 1256158(+) length=182 GL50803_14938 20 kda 100% 4 Ribosomal protein S3 location=glchr02:1088732 1089385(+) length=217 GL50803_7999 25 kda 100% 100% 100% 3 1 1 Hypothetical protein location=glchr05:3410142 3413099(+) length=985 GL50803_5183 106 kda 100% 3 Macrophage migration inhibitory factor location=glchr04:74459 74803( ) GL50803_12091 12 kda 100% 5 Ribosomal protein S11 location=glchr02:38668 39132( ) length=154 GL50803_14827 18 kda 99% 100% 96% 1 3 1 Histone H3 location=glchr03:411293 411772( ) length=159 GL50803_3367 18 kda 100% 2 ADP ribosylation factor location=glchr05:3986169 3986744(+) length=191 GL50803_7789 22 kda 96% 100% 1 3 ATP dependent RNA helicase location=glchr05:1736975 1739677(+) length=900 GL50803_90950 100 kda 100% 6

Hypothetical protein location=glchr05:1320864 1322075(+) length=403 GL50803_8415 46 kda 100% 5 Hypothetical protein location=glchr05:3285600 3286136( ) length=178 GL50803_17195 19 kda 100% 1 Ribosomal protein S21 location=glchr04:600401 600670(+) length=89 GL50803_7082 10 kda 100% 1 Protein F17L21.10 location=glchr05:2505627 2505995(+) length=122 GL50803_6724 13 kda 100% 2 Spastin location=glchr01:1010468 1013824( ) length=1118 GL50803_94322 123 kda 100% 100% 100% 100% 100% 5 16 2 6 1 Ribosomal protein L10 location=glchr04:1363733 1364365( ) length=210 GL50803_10428 24 kda 100% 100% 99% 100% 100% 100% 1 2 1 3 2 3 Pyruvate flavodoxin oxidoreductase location=glchr02:1109298 1113059(+) GL50803_114609 139 kda 100% 100% 1 15 Hypothetical protein location=glchr04:921032 924682(+) length=1216 GL50803_86815 135 kda 100% 100% 100% 100% 100% 2 9 4 3 1 Ribosomal protein S23 location=glchr05:1338019 1338450( ) length=143 GL50803_14699 16 kda 100% 99% 100% 100% 4 1 1 3 Ribosomal protein L23 location=glchr05:1285238 1285666( ) length=142 GL50803_10091 15 kda 100% 100% 100% 100% 100% 100% 1 2 2 1 1 1 Histone H4 location=ch991767:467222 467521( ) length=99 GL50803_135003 11 kda 100% 100% 99% 100% 2 3 1 4 Protein 21.6 location=glchr04:1286694 1289258( ) length=854 GL50803_17005 96 kda 95% 100% 100% 100% 1 3 4 9 Ribosomal protein L21 location=glchr03:1101248 1101727(+) length=159 GL50803_15520 18 kda 100% 100% 95% 4 2 1 Acetyl CoA synthetase location=glchr03:1776507 1778687( ) length=726 GL50803_13608 78 kda 100% 11 Ribosomal protein L34 location=glchr04:1421273 1421635( ) length=120 GL50803_36069 13 kda 95% 100% 100% 100% 1 4 1 1 Hypothetical protein location=glchr05:2360937 2361416( ) length=159 GL50803_7204 19 kda 100% 99% 9 1 Elongation factor 2 location=glchr04:2670898 2673594(+) length=898 GL50803_17570 100 kda 100% 100% 2 7 Axoneme associated protein GASP 180 location=glchr05:2250354 2255111( ) GL50803_137716 175 kda 100% 11 Peptidyl prolyl cis trans isomerase B precursor location=glchr01:556210 556716(+) GL50803_17163 18 kda 100% 100% 5 1 Nucleoside diphosphate kinase location=glchr04:1423378 1423833(+) length=151 GL50803_11301 17 kda 100% 6 ATP dependent RNA helicase location=glchr04:1739901 1741778( ) length=625 GL50803_13791 71 kda 100% 100% 100% 1 8 4 Ribosomal protein L18a location=glchr05:3966123 3966644( ) length=173 GL50803_16387 21 kda 100% 3 Wos2 protein location=glchr04:1363139 1363696(+) length=185 GL50803_10429 21 kda 100% 95% 3 1 Nucleolar GTPase location=glchr04:491207 492832(+) length=541 GL50803_16498 60 kda 100% 10 Acyl CoA synthetase location=glchr04:754005 756722( ) length=905 GL50803_16667 99 kda 100% 8 Hypothetical protein location=glchr04:189989 190834( ) length=281 GL50803_11354 30 kda 100% 96% 100% 4 1 2 Adenine phosphoribosyltransferase location=glchr01:1117274 1117816(+) GL50803_112885 19 kda 100% 4 Histone H3 location=glchr04:582567 583136( ) length=189 GL50803_14212 21 kda 100% 100% 100% 100% 100% 100% 100% 100% 1 2 1 5 1 3 1 1 Ribosomal protein L3 location=glchr04:2485220 2486359(+) length=379 GL50803_16525 43 kda 100% 100% 100% 100% 100% 2 2 1 12 2 Hypothetical protein location=glchr05:1459747 1460850( ) length=367 GL50803_7444 41 kda 100% 100% 100% 100% 100% 1 7 3 5 1 Hypothetical protein location=glchr02:1172398 1173243(+) length=281 GL50803_7566 31 kda 100% 100% 100% 100% 100% 100% 1 5 4 1 7 1 Hypothetical protein location=glchr05:977603 978016(+) length=137 GL50803_16298 15 kda 100% 100% 100% 100% 100% 100% 100% 2 1 5 0 1 1 2 Ribosomal protein L2 location=glchr05:1431827 1432582(+) length=251 GL50803_16086 27 kda 100% 100% 1 5 BirA GL50803_20130226 35 kda 100% 100% 100% 99% 100% 100% 4 2 2 1 4 3 Giardia trophozoite antigen GTA 1 location=glchr03:1714568 1715113( ) GL50803_17090 21 kda 100% 100% 100% 100% 100% 1 1 1 2 3 Protein 21.1 location=glchr05:2661167 2663317(+) length=716 GL50803_11164 79 kda 100% 100% 100% 2 4 3 Ribosomal protein S14 location=glchr04:1507409 1507846(+) length=145 GL50803_7878 16 kda 100% 100% 100% 1 5 3 Pyruvate, phosphate dikinase location=glchr04:1607101 1609755( ) length=884 GL50803_9909 98 kda 100% 12 Uracil phosphoribosyltransferase location=glchr05:1664106 1664741( ) length=211 GL50803_6563 23 kda 100% 100% 5 2 Ribosomal protein L27 location=glchr03:808135 808542(+) length=135 GL50803_8462 15 kda 100% 95% 100% 2 1 4 Hypothetical protein location=glchr05:3564268 3568260(+) length=1330 GL50803_101278 150 kda 100% 12 Ribosomal protein S15A location=glchr04:2164751 2165143( ) length=130 GL50803_15228 15 kda 100% 100% 3 5 Acyl CoA synthetase location=glchr03:1552447 1555359(+) length=970 GL50803_86511 107 kda 100% 9 Ribosomal protein S26 location=glchr04:2055996 2056325( ) length=109 GL50803_17364 13 kda 96% 100% 100% 100% 1 4 3 4 Acidic ribosomal protein P0 location=glchr05:1006646 1007626(+) length=326 GL50803_17054 35 kda 100% 98% 100% 2 1 2 GTP binding nuclear protein RAN/TC4 location=glchr05:1308332 1309012(+) GL50803_15869 25 kda 100% 100% 100% 2 3 1 Ribosomal protein L37a location=glchr05:3137049 3137333(+) length=94 GL50803_5517 10 kda 100% 100% 100% 100% 100% 100% 100% 2 1 1 3 1 1 2 Hypothetical protein location=glchr01:931872 933560(+) length=562 GL50803_5883 63 kda 100% 100% 100% 100% 100% 2 2 5 7 1 Hypothetical protein location=glchr05:4056196 4058676(+) length=826 GL50803_17011 93 kda 100% 100% 100% 100% 1 5 4 7 Ribosomal protein S18 location=glchr01:1044604 1045068( ) length=154 GL50803_15551 17 kda 100% 100% 100% 1 6 2 Cytosolic HSP70 location=glchr01:313398 315392(+) length=664 GL50803_88765 72 kda 100% 92% 12 1 Carbamate kinase location=glchr02:366273 367223(+) length=316 GL50803_16453 34 kda 100% 100% 100% 3 11 2 Phosphoglycerate kinase location=glchr02:165792 167021( ) length=409 GL50803_90872 44 kda 100% 100% 11 1 Fructose bisphosphate aldolase location=glchr03:1152763 1153734(+) length=323 GL50803_11043 35 kda 100% 10 Malate dehydrogenase location=glchr02:769888 770883(+) length=331 GL50803_3331 35 kda 100% 100% 95% 2 4 1

Thioredoxin reductase location=glchr02:678256 679200( ) length=314 GL50803_9827 34 kda 99% 100% 100% 1 7 1 Nucleolar GAR1 like protein, putative location=glchr02:591354 591905(+) GL50803_8794 20 kda 100% 95% 4 1 Protein 21.1 location=glchr05:2176256 2179330( ) length=1024 GL50803_9720 113 kda 100% 100% 100% 100% 100% 6 14 1 8 5 Ribosomal protein L4 location=glchr05:2867111 2868061( ) length=316 GL50803_17547 35 kda 100% 100% 100% 100% 100% 100% 3 2 1 11 1 2 NADP specific glutamate dehydrogenase location=glchr04:171236 172585( ) GL50803_21942 50 kda 100% 100% 1 20 Ribosomal protein L23A location=glchr04:1500461 1500886( ) length=141 GL50803_7870 16 kda 100% 100% 100% 100% 100% 100% 1 2 3 7 4 1 Hypothetical protein location=glchr05:3071638 3073458(+) length=606 GL50803_7891 66 kda 100% 100% 100% 100% 100% 4 5 5 3 3 Kinase, NEK location=glchr02:126363 127910(+) length=515 GL50803_15409 57 kda 95% 100% 99% 1 11 1 Protein 21.1 location=glchr05:2134897 2137407(+) length=836 GL50803_16532 93 kda 100% 100% 100% 100% 2 2 6 3 Kelch repeat containing protein location=glchr05:1716052 1720596( ) length=1514 GL50803_15054 166 kda 100% 7 Hypothetical protein location=glchr04:467449 471141(+) length=1230 GL50803_16503 135 kda 100% 100% 100% 100% 100% 5 13 7 9 2 Ribosomal protein S24 location=glchr04:1129929 1130327(+) length=132 GL50803_10367 15 kda 100% 100% 100% 100% 100% 2 1 6 2 4 Glyceraldehyde 3 phosphate dehydrogenase location=glchr05:3056628 3057638( ) GL50803_6687 36 kda 100% 100% 100% 1 16 1 Peroxiredoxin 1 location=glchr03:1256829 1257434( ) length=201 GL50803_14521 23 kda 100% 100% 100% 100% 96% 100% 100% 4 1 1 6 1 3 2 Ribosomal protein S5 location=ch991769:654358 654930(+) length=190 GL50803_12981 21 kda 96% 99% 100% 100% 1 1 10 2 Ribosomal protein S8 location=glchr03:910371 910895( ) length=174 GL50803_5845 19 kda 100% 100% 100% 100% 100% 100% 2 9 4 4 1 4 Ribosomal protein S9 location=glchr01:949940 950509( ) length=189 GL50803_4547 22 kda 100% 100% 96% 100% 1 8 1 1 Ribosomal protein S6 location=glchr01:1441844 1442590(+) length=248 GL50803_14620 28 kda 100% 100% 100% 100% 100% 100% 1 2 6 13 3 2 Ribosomal protein S13 location=glchr05:3812245 3812709( ) length=154 GL50803_16652 18 kda 100% 100% 100% 100% 6 2 1 1 Ribosomal protein L17 location=glchr05:3739089 3739583(+) length=164 GL50803_98056 19 kda 100% 100% 100% 1 9 2 Ribosomal protein L18 location=glchr04:895590 896129(+) length=179 GL50803_11950 20 kda 95% 100% 100% 100% 100% 100% 1 1 2 10 4 2 Protein 21.1 location=glchr03:1597805 1601245( ) length=1146 GL50803_95192 124 kda 100% 100% 10 2 Enolase location=glchr05:2034587 2035924(+) length=445 GL50803_11118 48 kda 100% 100% 100% 4 18 2 Xaa Pro dipeptidase location=glchr01:731223 732557(+) length=444 GL50803_17327 50 kda 100% 100% 95% 1 17 1 Glycerol 3 phosphate dehydrogenase location=glchr05:2522245 2525580( ) GL50803_16125 119 kda 100% 17 Ribosomal protein L26 location=glchr04:25780 26187(+) length=135 GL50803_15046 16 kda 100% 100% 100% 100% 100% 100% 2 2 12 3 6 4 Spindle pole protein, putative location=glchr04:1062992 1065334(+) length=780 GL50803_17075 85 kda 100% 100% 100% 100% 100% 9 14 9 13 5 Ribosomal protein S17 location=glchr05:4086927 4087340( ) length=137 GL50803_6135 16 kda 100% 100% 9 6 UPL 1 location=glchr04:22438 23370(+) length=310 GL50803_9779 34 kda 100% 100% 100% 13 11 7 Ribosomal protein S16 location=glchr01:702428 702904(+) length=158 GL50803_4652 18 kda 100% 95% 100% 100% 100% 1 1 11 2 0 4 Ribosomal protein L19 location=glchr02:91793 92383( ) length=196 GL50803_16431 23 kda 100% 100% 100% 100% 100% 100% 1 1 11 14 6 0 2 Ribosomal protein L13 location=glchr01:1437382 1438086(+) length=234 GL50803_14622 27 kda 100% 100% 100% 100% 100% 100% 100% 1 1 2 10 17 3 2 0 Alpha tubulin location=glchr03:1813742 1815106( ) length=454 GL50803_112079 51 kda 100% 100% 100% 100% 100% 100% 100% 100% 5 1 4 6 19 11 1 4 Arginine deiminase location=glchr03:1755615 1757357( ) length=580 GL50803_112103 64 kda 100% 100% 100% 4 27 1 Beta tubulin location=glchr05:3604400 3605743( ) length=447 GL50803_101291 50 kda 100% 100% 100% 100% 100% 100% 100% 5 2 4 8 19 7 4 Kinase, NEK location=glchr02:581481 584834(+) length=1117 GL50803_15953 125 kda 100% 100% 100% 100% 100% 8 18 8 21 1 Hypothetical protein location=glchr05:2095138 2096601(+) length=487 GL50803_92760 55 kda 100% 100% 100% 100% 100% 14 14 14 20 4 Hypothetical protein location=glchr05:2092097 2095063(+) length=988 GL50803_16050 110 kda 100% 100% 100% 100% 100% 2 12 8 20 3 Elongation factor 1 alpha location=glchr02:27919 29247(+) length=442 GL50803_112304 49 kda 100% 100% 100% 100% 100% 100% 100% 4 1 2 5 16 3 2 Trichohyalin location=glchr04:2665055 2668135( ) length=1026 GL50803_17571 116 kda 100% 100% 100% 100% 100% 5 20 11 24 1 Ribosomal protein L27a location=glchr05:835577 836026(+) length=149 GL50803_16310 17 kda 100% 100% 100% 100% 96% 100% 100% 100% 2 6 1 9 1 9 2 3 FtsJ cell division protein, putative location=glchr05:2477561 2480815(+) GL50803_16993 123 kda 100% 100% 100% 4 1 32 Ornithine carbamoyltransferase location=glchr03:1022938 1023921(+) length=327 GL50803_10311 36 kda 100% 100% 100% 100% 1 14 23 6 ATP/GTP binding protein, putative location=glchr04:1125269 1126630(+) GL50803_10370 53 kda 100% 100% 100% 100% 100% 100% 100% 95% 9 9 6 2 16 16 5 1 Coiled coil protein location=glchr02:1413847 1417971(+) length=1374 GL50803_9515 150 kda 100% 100% 100% 100% 3 1 18 5 Spindle pole protein, putative location=glchr01:308747 311734( ) length=995 GL50803_7031 109 kda 100% 100% 100% 100% 100% 4 27 1 29 9 Hypothetical protein location=glchr01:793818 794975( ) length=385 GL50803_9861 43 kda 100% 100% 100% 100% 100% 100% 1 3 2 5 32 3 GiOR1 G. intestinalis strain expressing cytosolic biotine ligase (cbira) and GiOR1 fused with biotin acceptor peptide Cia2 G. intestinalis strain expressing cytosolic biotine ligase (cbira) and Cia2 fused with biotin acceptor peptide cbira G. intestinalis strain expressing cbira (control) wt parent G. intestinalis strain (control)