(1) Leishmania infantum zoonotic L. donovani anthroponotic 1 1. Leishmania infantum L. donovani. (2) 4 TNF-α IL-10 (aly/aly) Foxp3 + CD4 + T (T, Treg) L. infantum L. donovani Treg (3) 30-100 nm L. donovani (1) (2) T (Treg)(3) (1) 50 2 2.
(Kalazar Detect TM, rk39 dipstick) rk39 DNA DNA ITS PCR (ITS-PCR) (2) 1 3 L. donovani promastigote 4.3 x 10 9 13-01491 1, 3, 5 8 8 (Alb, A/G, GOP, GPT, γ-gt, UN, CRE)DNA ITS-PCR DNA (kdna) real-time PCR (kdna-qpcr) CD4 + T cell CD8 + T cellcd25 + T cellfoxp3 + PCR 2 1 2 2 4 (3) L. donovani 34 (ExoQuick TM ) (MALDI-TOF-MS) (1) 50 6 12rk39 3 5 10ITS-PCR 4 3. rk39 dipstick. 4. ITS-PCR DNA. 9 18 3 (2) P2 1 3 3 8 ITS-PCR kdna-qpcr CD4 + CD25 + Foxp3 + (Treg)CD4 + T 6 5
5. CD4 + CD25 + Foxp3 + (Treg)CD4 + T. 3 HE 3 1 90 kda 8 690 kda 6.. (PC).1235 8 01m2m3m5m8m 100 2 HRP protein G ECL Plus TM. 2 1 2 4 PCR 2 DNA 8 L. donovani (3) L. donovani MALDI-TOF-MS Mascot Search 26 11 probable eukaryotic inhibition factor 4A, 40S ribosomal protein SA, probable 60S ribosomal protein L14, heat shock 70 kda protein, receptor-type adenylate cyclase A, S-adenosylmethionine synthase, elongation factor Ts (mitochondrial), histon H3, kinesin-like protein K39 (fragment), leishmanolysin, protein phosphatase 2C 15 (LC-MS/MS) (GO) 12 Terao M, Akter S, Yasin Md.G, Nakao R, Kato H, Alam MZ, Katakura K: Molecular detection and genetic diversity of Babesia gibsoni in dogs in Bangladesh. Infect Genet Evol 31:53-60, 2015. doi: 10.1016/j.meegid.2015.01.011 Ikeda T, Yoshimura M, Onoyama K, Oku Y, Nonaka N, Katakura K: Where to deliver baits for deworming urban red foxes for Echinococcus multilocularis control: new protocol for micro-habitat modeling of fox denning requirements. Parasit Vectors
7(1):357, 2014. doi:10.1186/1756-3305-7-357 Alam MZ, Bhutto AM, Soomro FR, Baloch JH, Nakao R, Kato H, Schönian G, Uezato H, Hashiguchi Y, Katakura K: Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing. Parasit Vectors 7(1): 332, 2014. doi: 10.1186/1756-3305-7-332. Chiba R, Amagai Y, Tanaka A, Katakura K, Matsuda H.: Nerve growth factor promotes killing of Leishmania donovani by macrophages through the induction of hydrogen peroxide. Microbes Infect 16(8): 702-706, 2014. doi: 10.1016/ j.micinf.2014.06.001 Bawm S, Shimizu K, Hirota J, Tosa Y, Htun LL, Maw NN, Thein M, Kato H, Sakurai T, Katakura K: Molecular prevalence and genetic diversity of bovine Theileria orientalis in Myanmar. Parasitol Int 63(4): 640-645, 2014. doi: 10.1016/j.parint.2014.04.009 Alam MZ, Nakao R, Sakurai T, Kato H, Qu J-Q, Chai J-J, Chang K-P, Schönian G, Katakura K: Genetic diversity of Leishmania donovani/infantum complex in China through microsatellite analysis. Infect Genet Evol 22, 112-119, 2014. doi: 10.1016/j.meegid.2014.01.019 Nzelu CO, Gomez EA, Cáceres AG, Sakurai T, Martini-Robles L, Uezato H, Mimori T, Katakura K, Hashiguchi Y, Kato H: Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection. Acta Trop 132:1-6, 2014. doi: 10.1016/j.actatropica.2013.12.016. : III.40 (4) 347-352, 2013. https://www.kindai-s.co.jp/products/list.php? category_id=37 :. 11 (2) 61-70, 2013. http://jsvp.umin.jp/kaishi.html Alam MZ, Yasin MG, Kato H, Sakurai T, Katakura K: PCR-based detection of Leishmania donovani DNA in a stray dog from a visceral leishmaniasis endemic focus in Bangladesh. J Vet Med Sci 75, 75-78, 2013. doi:10.1292/jvms.12-0134 Tiwananthagorn S, Iwabuchi K, Ato M, Sakurai T, Kato H, Katakura K: Involvement of CD4 + Foxp3 + regulatory T cells in persistence of Leishmania donovani in the liver of alymphoplastic aly/aly mice. PLoS Negl Trop Dis 6, e1798, 2012. doi:10.1371/journal.pntd.0001798 Tiwananthagorn S, Bhutto AM, Baloch JH, Soomro FR, Kawamura Y, Nakao R, Aoshima K, Nonaka N, Oku Y, Katakura K: Zoophilic feeding behaviour of phlebotomine sand flies in the endemic areas of cutaneous leishmaniasis of Sindh Province, Pakistan. Parasitol Res 111, 125-133, 2012. doi:10.1007/s00436-011-2808-3 8, Alam M,,,, Chang KP, Schönian G: Population genetics of Leishmania donovani/ infantum complex in China by multilocus microsatellite analysis. 83 2014 3 27 Katakura K: Molecular epidemiological studies on livestock parasites in Myanmar. First International Conference and the 13 th Annual Meeting of Myanmar Veterinary Association, 2014 2 2, Yangon Convention Center, Yangon, Myanmar Alam MZAkter S. 156 2013 9 21 Alam MZ, Nakao R, Sakurai T, Kato H, Chang KP, Schönian G, Katakura K Multilocus microsatellite typing of Leishmania infantum parasites from China. Fifth World Congress on Leishmaniasis. 2013 5 14 Enotel Resort & Spa, Porto de Galinhas. 82 2013 3 31 Alam MZ, Akter S, Yasin Md.G, Serological and molecular evidence of Leishmania parasite in dogs of visceral leishmaniasis endemic areas in Bangladesh. 155 2013 3 28 Alam M,,,, Chang KP, Schönian G, Genetic
polymorphism of Chinese Leishmania infantum strains revealed by multilocus microsatellite analysis. 154 2012 9 16 Tiwananthagorn S aly/aly T. 154 2012 9 16 3 II 2014331 (pp.126-130) http://www.vetmed.hokudai.ac.jp/parasitology01. html (1) (KATAKURA, Ken) 10130155 (2) (KATO, Hirotomo) 50281853 (3) (TAKIGUCHI, Mitsuyoshi) 70261336 (ATO, Manabu) 20392318