Legends for Supplemental Data

Legends for Supplemental Data Supplemental Fig. 1. Top molecular and cellular functions identified by Ingenuity Pathway Analysis after 6 h (left) and 24 h (right) of exposure of mouse pancreatic islets to IL-1β and IFN-γ (p<0.05 by Fischer s exact test). Supplemental Fig. 2. Canonical pathways identified by Ingenuity Pathway Analysis after 6 h (left) and 24 h (right) of exposure of mouse pancreatic islets to IL-1β and IFN-γ (p<0.05 by Fischer s exact test). Supplemental Fig. 3. Top molecular and cellular functions identified by Ingenuity Pathway Analysis after 6 h (left) and 24 h (right) of co-treatment (1,25(OH) 2 D 3 in combination with IL-1β and IFN-γ) as compared to cytokine exposure alone (IL-1β and IFN-γ) of mouse pancreatic islets (p<0.05 by Fischer s exact test). Supplemental Fig. 4. Effect of 1,25(OH) 2 D 3 on qrt-pcr mrna expression of chemokines and cytokines in IL-1β- and IFN-γ-treated human islets. Islets were cultured for 24 h with ) control medium; ) 10-8 M 1,25(OH) 2 D 3 ; ) 50 U/ml IL-1β and 100 U/ml IFN-γ; and ) 10-8 M 1,25(OH) 2 D 3, 50 U/ml IL-1β and 100 U/ml IFN-γ. Data are presented as three individual islet isolations (HI 1, HI 2 and HI 3). Supplemental Table 1. Manual classification of selected genes differentially regulated by IL-1β and IFN-γ vs vehicle in islets after 6 and 24 h of in vitro exposure (n=4 independent experiments expressed as fold change vs respective control. *p<0.05; **p<0.02; ***p<0.01; ****p<0.001 vs vehicle by Welch s t-test. Con: Control. 1,25: 1,25(OH) 2 D 3. Cyt: IL-1β and IFN-γ). Supplemental Table 2. Manual classification of selected genes differentially regulated by cotreatment (1,25(OH) 2 D 3 in combination with IL-1β and IFN-γ) as compared to cytokine exposure alone (IL-1β and IFN-γ) in pancreatic islets (n=4 independent experiments expressed as fold change vs respective control. *p<0.05; **p<0.02; ***p<0.01; ****p<0.001 Welch s t-test. Con: Control. 1,25: 1,25(OH) 2 D 3. Cyt: IL-1β and IFN-γ).

1 Supplemental materials and methods 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Chemotaxis assay C57BL/6 islets were cultured in BioWhittaker UltraCULTURE Serum-Free Medium (Lonza, Basel, Switzerland) supplemented with Glutamax -I (Life Technologies, Carlsbad, CA) and 5 µg/ml geneticin. Exposure to 1,25(OH) 2 D 3 and cytokines was similar to that described in section 2.2. except for a prolonged culture period of 3 days. For the chemotaxis assay thioglycolate-elicited peritoneal cells from 8-12 week old C57BL/6 mice were seeded in the top chamber of Costar transwell plates (1 10 6 cells per well) (5.0 µm, Corning Inc., Corning, NY), while conditioned islet medium was added to the lower chambers of the transwell plate. Positive and negative controls consisted of UltraCULTURE Serum-Free medium with or without recombinant murine MIP-1α (50ng/ml, PeproTech), respectively. Following an incubation time of 2 h at 37 C in 5% CO 2, the cells in the lower chamber were harvested and stained for monocyte specific cell surface markers. To minimize nonspecific binding, the cells were first pre-incubated with anti-cd16/cd32 (clone 2.4G2). Cells were then labeled with directly conjugated mabs: CD45, CD11b, Ly6C, F4/80 (San Diego, CA, USA). Dead cells were excluded by using the Fixable Live/Dead Yellow stain according to the manufacturer s specifications (Invitrogen, Carlsbad, CA). The absolute number of migrated monocytes was determined by CountBright absolute counting beads (Invitrogen). Data acquisition was performed on a Gallios TM flow cytometer (Beckman Coulter, Brea, CA, USA) and KaluzaTM software was used for data analysis. Monocytes were determined as Ly6C + CD11b + F4/80 - within the viable leukocyte (CD45 + ) gate. 22 23 24 25 26 27 Assessment of NF-κB activity Islets were cultured for 24 h in the presence of 1,25(OH) 2 D 3 or vehicle, after which they were stimulated for 30 min with IL-1β and IFN-γ at 37 C. Thereafter, islets were washed twice in ice-cold PBS supplemented with 1:100 Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich, St. Louis, MO) and lysed in Complete lysis buffer (Active Motif, Carlsbad, CA). Protein concentrations were determined

28 29 by the Coomassie method. Binding activity of NF-κB p52 subunits was determined by the TransAM NF-κB assay according to the manufacturer s instructions (Active Motif).

6 h 24 h Cyt Cyt Accession Symbol Gene name / functional group vs Con vs Con Arginine metabolism, Nitric oxide formation NM_007482 Arg1 arginase, liver -6.12 **** -7.59 **** NM_010927 Nos2 (inos) nitric oxide synthase 2, inducible 148.83 **** 40.93 **** Glucose metabolism, Krebs cycle NM_007386 Aco1 aconitase 1-1.35 **** -1.27 **** NM_080633 Aco2 aconitase 2, mitochondrial -1.38 **** -1.37 *** NM_009656 Aldh2 aldehyde dehydrogenase 2, mitochondrial -1.39 *** -1.43 *** NM_026444 Cs citrate synthase 1.05 1.19 * NM_023119 Eno1 enolase 1, alpha non-neuron -1.29 **** 1.38 *** NM_010209 Fh1 fumarate hydratase 1 1.17 **** -1.91 **** NM_010292 Gck glucokinase 1.05-1.46 *** NM_008133 Glud1 glutamate dehydrogenase 1-1.35 **** 1.01 NM_008149 Gpam glycerol-3-phosphate acyltransferase, mitochondrial -1.63 *** 1.06 NM_010271 Gpd1 glycerol-3-phosphate dehydrogenase 1 (soluble) -2.24 **** 1.07 NM_008155 Gpi1 glucose phosphate isomerase 1-1.28 ** 1.3 ** NM_013820 Hk2 hexokinase 2 3.65 **** 2.78 *** NM_010497 Idh1 isocitrate dehydrogenase 1 (NADP+), soluble -1.49 **** -2.42 **** NM_173011 Idh2 isocitrate dehydrogenase 2 (NADP+), mitochondrial -2.01 **** 1.45 *** NM_130884 Idh3b isocitrate dehydrogenase 3 (NAD+), beta -1.14 **** 1.14 ** NM_008323 Idh3g isocitrate dehydrogenase 3 (NAD+), gamma -1.22 * -1.32 **** NM_010699 Ldha lactate dehydrogenase A 1.11 1.94 ** NM_027570 Ldhd lactate dehydrogenase D 1.38 *** -1.02 NM_008618 Mdh1 malate dehydrogenase 1, NAD (soluble) -1.19 **** -1.4 **** NM_008617 Mdh2 malate dehydrogenase 2, NAD (mitochondrial) -1.13 * -1.07 ** NM_010956 Ogdh oxoglutarate dehydrogenase (lipoamide) -1.29 *** -1.85 **** NM_011032 P4hb prolyl 4-hydroxylase, beta polypeptide -1.24 **** -1.18 * NM_008797 Pcx pyruvate carboxylase -1.43 *** -1.98 *** NM_008810 Pdha1 pyruvate dehydrogenase E1 alpha 1-1.92 *** -3.46 **** NM_024221 Pdhb pyruvate dehydrogenase (lipoamide) beta -1.44 **** -1.14 NM_172665 Pdk1 pyruvate dehydrogenase kinase, isoenzyme 1-1.53 *** -1.04 NM_133667 Pdk2 pyruvate dehydrogenase kinase, isoenzyme 2-2.34 **** -1.96 *** NM_145630 Pdk3 pyruvate dehydrogenase kinase, isoenzyme 3 1.49 *** 1.54 ** NM_013743 Pdk4 pyruvate dehydrogenase kinase, isoenzyme 4-5.19 **** -9.02 **** NM_008825 Pfkfb2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2-1.6 *** -2.37 *** NM_133232 Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (glycolytic 9.8 **** 4.08 **** activator) NM_008826 Pfkl phosphofructokinase, liver, B-type -1.64 **** 1.46 * NM_021514 Pfkm phosphofructokinase, muscle -2.15 **** -2 **** NM_019703 Pfkp phosphofructokinase, platelet 6 **** 2.67 *** NM_028273 Pgam5 phosphoglycerate mutase family member 5 1.09 *** 1.54 *** NM_013631 Pklr pyruvate kinase liver and red blood cell -1.53 *** -1.54 *** NM_011099 Pkm2 pyruvate kinase, muscle -1.35 *** 1.03 NM_023374 Sdhb succinate dehydrogenase complex, subunit B, iron sulfur (Ip) -1.15 * 1.06 NM_011400 Slc2a1 solute carrier family 2 (facilitated glucose transporter), member 1 1.91 *** 4.92 *** NM_031197 Slc2a2 (Glut-2) solute carrier family 2 (facilitated glucose transporter), member 2-11.77 **** -7.81 **** NM_019879 Suclg1 succinate-coa ligase, GDP-forming, alpha subunit 1.08-1.15 NM_009415 Tpi1 triosephosphate isomerase 1-1.27 **** 1.38 ** Lipid metabolism NM_133360 Acaca acetyl-coenzyme A carboxylase alpha -1.82 *** -1.53 **** NM_144784 Acat1 acetyl-coenzyme A acetyltransferase 1-1.78 **** -2.02 **** NM_134037 Acly ATP citrate lyase -1.65 **** -1.77 **** NM_007981 Acsl1 acyl-coa synthetase long-chain family member 1-1.11-1.27 *** NM_028817 Acsl3 acyl-coa synthetase long-chain family member 3-1.47 *** 1.3 **** NM_027976 Acsl5 acyl-coa synthetase long-chain family member 5 1.76 **** 1.52 **** NM_021273 Ckb creatine kinase, brain -1.32 * -1.98 **** NM_009897 Ckmt1 creatine kinase, mitochondrial 1, ubiquitous -1.2 * 1.74 * NM_013495 Cpt1a carnitine palmitoyltransferase 1a, liver 1.14 ** 2.14 **** NM_009949 Cpt2 carnitine palmitoyltransferase 2-1.07 1.04 NM_198114 Dagla diacylglycerol lipase, alpha -2.37 **** -1.7 * NM_138650 Dgkg diacylglycerol kinase, gamma 7.43 **** 2.08 *

NM_019423 Elovl2 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, -1.87 *** -2.58 **** yeast)-like 2 NM_130450 Elovl6 ELOVL family member 6, elongation of long chain fatty acids 1.18 ** -1.87 ** (yeast) NM_146094 Fads1 fatty acid desaturase 1-2.2 **** -1.65 **** NM_019699 Fads2 fatty acid desaturase 2-2.23 **** -2.41 * NM_007988 Fasn fatty acid synthase -2.08 **** -1.93 **** NM_212444 Gyk glycerol kinase -1.35 ** -1.47 **** NM_008280 Lipc lipase, hepatic 1.51 **** 1.65 **** NM_008509 Lpl lipoprotein lipase -1.41 *** -1.15 ** NM_033218 Srebf2 sterol regulatory element binding factor 2-1.19 * -1.31 * NM_013703 Vldlr very low density lipoprotein receptor -1 1.01 Interferon-γ signaling NM_010509 Ifnar2 interferon (alpha and beta) receptor 2 1.98 **** 2.52 **** NM_010511 Ifngr1 interferon gamma receptor 1 1.06 1.26 NM_008338 Ifngr2 interferon gamma receptor 2 5.58 **** 3.03 **** NM_008390 Irf1 interferon regulatory factor 1 18.51 **** 9.97 **** NM_008390 Irf1 interferon regulatory factor 1 1.95 **** 1.64 **** NM_008391 Irf2 interferon regulatory factor 2 1.67 **** 1.47 **** NM_016849 Irf3 interferon regulatory factor 3-1 1.02 NM_016850 Irf7 interferon regulatory factor 7 13.5 **** 13.27 **** NM_008320 Irf8 interferon regulatory factor 8 4.33 **** 2.29 **** NM_001159417 Irf9 interferon regulatory factor 9 6.43 **** 5.79 **** NM_146145 Jak1 Janus kinase 1 1.7 **** 1.49 **** NM_008413 Jak2 Janus kinase 2 4.11 **** 2.02 *** NM_010589 Jak3 Janus kinase 3 1.95 **** 1.52 *** NM_009896 Socs1 suppressor of cytokine signaling 1 2.09 **** 1.48 **** NM_007706 Socs2 suppressor of cytokine signaling 2-1.1-1.52 U72673 Socs3 suppressor of cytokine signaling 3 1.41 * 1.04 NM_007707 Socs3 suppressor of cytokine signaling 3 3.17 **** 1.41 ** NM_018821 Socs6 suppressor of cytokine signaling 6 1.19 * 1.47 * NM_138657 Socs7 suppressor of cytokine signaling 7 1.08 1.45 *** NM_009283 Stat1 signal transducer and activator of transcription 1 14.22 **** 11.51 **** NM_019963 Stat2 signal transducer and activator of transcription 2 8.03 **** 7.85 **** NM_213659 Stat3 signal transducer and activator of transcription 3 3.13 **** 2.1 *** NM_001113563 Stat5b signal transducer and activator of transcription 5B -1.03 1.17 ** NM_009284 Stat6 signal transducer and activator of transcription 6 1.73 **** 1.63 *** NF-κB regulation NM_019777 Ikbke inhibitor of kappab kinase epsilon 9.22 **** 2.99 **** NM_001161421 Ikbkg inhibitor of kappab kinase gamma 1.38 *** 2.14 **** NM_172161 Irak2 interleukin-1 receptor-associated kinase 2 2.06 **** 2.43 **** NM_028679 Irak3 interleukin-1 receptor-associated kinase 3 4.94 **** 1.92 **** NM_008689 Nfkb1 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, 3.36 **** 1.29 ** p105 NM_019408 Nfkb2 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, 10.76 **** 3.86 **** p49/p100 NM_010907 Nfkbia nuclear factor of kappa light polypeptide gene enhancer in B-cells 10.2 **** 5.43 **** inhibitor, alpha NM_010908 Nfkbib nuclear factor of kappa light polypeptide gene enhancer in B-cells 2.7 **** 1.95 **** inhibitor, beta NM_008690 Nfkbie nuclear factor of kappa light polypeptide gene enhancer in B-cells 3.26 **** 1.49 * inhibitor, epsilon NM_030612 Nfkbiz nuclear factor of kappa light polypeptide gene enhancer in B-cells 4.61 **** 2.69 **** inhibitor, zeta NM_011529 Tank TRAF family member-associated Nf-kappa B activator 1.91 **** 1.13 ** NM_019786 Tbk1 TANK-binding kinase 1 2.39 **** 1.64 *** NM_145133 Tifa TRAF-interacting protein with forkhead-associated domain 54.38 **** 7.59 **** NM_009397 Tnfaip3 (A20) tumor necrosis factor, alpha-induced protein 3 13.81 **** 3.32 **** NM_021327 Tnip1 TNFAIP3 interacting protein 1 (obs candidate gene in psoriarisis) 6.72 **** 4.97 **** NM_139064 Tnip2 TNFAIP3 interacting protein 2 1.48 *** 1.5 **** NM_009422 Traf2 TNF receptor-associated factor 2 1.6 **** 1.66 ** Other transcription factors NM_028967 Batf2 basic leucine zipper transcription factor, ATF-like 2 6.08 **** 3.39 **** NM_009740 Bcl10 B-cell leukemia/lymphoma 10 1.77 *** 1.32 ***

NM_033601 Bcl3 B-cell leukemia/lymphoma 3 6.58 **** 3.96 **** NM_009744 Bcl6 B-cell leukemia/lymphoma 6 2.72 **** 4.2 *** NM_175045 Bcor BCL6 interacting corepressor 1.17 1.22 *** NM_007553 Bmp2 bone morphogenetic protein 2 1.08 1.51 **** NM_009883 Cebpb CCAAT/enhancer binding protein (C/EBP), beta 2.79 **** 1.88 **** NM_007679 Cebpd CCAAT/enhancer binding protein (C/EBP), delta 11.08 **** 5 **** NM_009884 Cebpg CCAAT/enhancer binding protein (C/EBP), gamma 1.62 **** 1.63 *** NM_007865 Dll1 delta-like 1 (Drosophila) 3.99 **** 1.98 *** NM_010234 Fos FBJ osteosarcoma oncogene 1.02 1.03 NM_019739 Foxo1 forkhead box O1-1.6 **** -1.35 **** NM_018789 Foxo4 forkhead box O4-1.39 **** -1.49 **** NM_019479 Hes6 hairy and enhancer of split 6 (Drosophila) -1.67 **** -1.36 *** NM_010431 Hif1a hypoxia inducible factor 1, alpha subunit 2.27 **** 1.24 * NM_026820 Ifitm1 interferon induced transmembrane protein 1 2.57 **** 1.48 **** NM_021459 Isl1 ISL1 transcription factor, LIM/homeodomain -1.53 **** -3.63 **** NM_010588 Jag2 jagged 2-1.44 *** -1.62 **** NM_010591 Jun (c-jun) Jun oncogene 5.86 **** 4.13 *** NM_008416 Junb Jun-B oncogene 2.72 **** 1.9 *** NM_010592 Jund Jun proto-oncogene related gene d 1.53 *** 1.4 *** NM_009769 Klf5 Kruppel-like factor 5 2.66 **** 1.51 *** NM_011803 Klf6 Kruppel-like factor 6 2.13 **** 2.62 *** NM_010894 Neurod1 neurogenic differentiation 1-1.12-2.15 *** NM_009719 Neurog3 neurogenin 3-2.75 **** -2.44 *** NM_010919 Nkx2-2 NK2 transcription factor related, locus 2 (Drosophila) -2.09 **** -2.38 **** NM_144955 Nkx6-1 NK6 homeobox 1-1.98 **** -1.8 *** NM_013627 Pax6 paired box gene 6-1.35 **** -2.41 **** NM_008814 Pdx1 pancreatic and duodenal homeobox 1-2.62 **** -4.99 **** NM_011145 Ppard peroxisome proliferator activator receptor delta 1.55 **** 1.12 NM_153125 Sec16a SEC16 homolog A (S. cerevisiae) -1.1-1.39 *** NM_011480 Srebf1 sterol regulatory element binding transcription factor 1-1.73 *** -1.48 **** NM_001142918 Tcf7l2 transcription factor 7-like 2, T-cell specific, HMG-box 1.31 *** -1.37 **** NM_009396 Tnfaip2 tumor necrosis factor, alpha-induced protein 2 20.36 **** 5.76 **** NM_144551 Trib2 tribbles homolog 2 (Drosophila) -1.53 **** -1.39 **** NM_011915 Wif1 Wnt inhibitory factor 1 1.9 **** 1.74 *** NM_013842 Xbp1 X-box binding protein 1 1.13 *** -1.33 *** Splicing machinery, chromatin regulation NM_008229 Hdac2 histone deacetylase 2-1.37 *** -1.19 NM_010411 Hdac3 histone deacetylase 3-1.34 **** -1.36 *** NM_001077696 Hdac5 histone deacetylase 5-2.06 **** -1.24 *** NM_024124 Hdac9 histone deacetylase 9-2.15 **** -1.74 *** NM_009545 Pcgf2 polycomb group ring finger 2-1.31 *** 1.06 BC145590 Pcgf5 polycomb group ring finger 5 1.02-1.17 *** NM_173374 Sfrs1 splicing factor, arginine/serine-rich 1 (ASF/SF2) 1.01-2.18 **** NM_001081956 Sfrs17b splicing factor, arginine/serine-rich 17b -1.14-1.54 *** NM_025669 Sfrs18 splicing factor, arginine/serine-rich 18-1.48 *** -1.95 *** NM_025669 Sfrs18 splicing factor, arginine/serine-rich 18-1.43 **** -1.22 NM_011358 Sfrs2 splicing factor, arginine/serine-rich 2 (SC-35) 1.05-1.29 *** NM_028148 Sfrs2ip splicing factor, arginine/serine-rich 2, interacting protein -1.2 * 1.3 * NM_013663 Sfrs3 splicing factor, arginine/serine-rich 3 (SRp20) -1.04-2.65 ** NM_020587 Sfrs4 splicing factor, arginine/serine-rich 4 (SRp75) 1.47 **** 1.48 * NM_026499 Sfrs6 splicing factor, arginine/serine-rich 6-1.11-1.79 **** NM_146083 Sfrs7 splicing factor, arginine/serine-rich 7 1.14 * -1.11 ** Chemokines, cytokines, adhesion molecules NM_011330 Ccl11 (Eotaxin) chemokine (C-C motif) ligand 11 3.43 **** 1.77 NM_011333 Ccl2 (MCP-1) chemokine (C-C motif) ligand 2 70.4 **** 15.19 **** NM_016960 Ccl20 chemokine (C-C motif) ligand 20 4.35 **** 1.22 * NM_020279 Ccl28 chemokine (C-C motif) ligand 28 2.99 **** 1.23 ** NM_013653 Ccl5 (RANTES) chemokine (C-C motif) ligand 5 3.99 **** 4.21 **** NM_013654 Ccl7 (MCP-3) chemokine (C-C motif) ligand 7 3.13 *** 2.73 *** NM_021443 Ccl8 (MCP-2) chemokine (C-C motif) ligand 8 2.15 **** 3 **** NM_017466 Ccrl2 chemokine (C-C motif) receptor-like 2 10.16 **** 4.92 **** NM_007778 Csf1 colony stimulating factor 1 (macrophage) 5.13 **** 3.43 *** NM_009142 Cx3cl1 (fractalkine) chemokine (C-X3-C motif) ligand 1 1.34 **** -1.38 ****

NM_008176 Cxcl1 (Gro-α) chemokine (C-X-C motif) ligand 1 14.57 **** 4.06 **** NM_021274 Cxcl10 (IP-10) chemokine (C-X-C motif) ligand 10 417.32 **** 130.01 **** NM_019494 Cxcl11 (Itac) chemokine (C-X-C motif) ligand 11 43.34 **** 13.57 **** NM_023158 Cxcl16 chemokine (C-X-C motif) ligand 16 6.24 **** 8.57 **** NM_009140 Cxcl2 (Gro-beta) chemokine (C-X-C motif) ligand 2 23.18 **** 2.78 * NM_009141 Cxcl5 (ENA-78) chemokine (C-X-C motif) ligand 5 6.73 **** 13.64 **** NM_008599 Cxcl9 (Mig) chemokine (C-X-C motif) ligand 9 203.3 **** 191.01 **** NM_010493 Icam1 intercellular adhesion molecule 1 30.38 **** 11.77 **** NM_008349 Il10rb interleukin 10 receptor, beta 1.84 **** 1.45 **** NM_133990 Il13ra1 interleukin 13 receptor, alpha 1 4.56 **** 3.1 **** NM_008357 Il15 interleukin 15 2.55 **** 1.64 ** NM_008358 Il15ra interleukin 15 receptor, alpha chain 2 **** 1.66 *** NM_145834 Il17c interleukin 17C 2.1 *** -1.01 NM_134159 Il17rc interleukin 17 receptor C 1.34 *** 1.44 **** NM_134437 Il17rd interleukin 17 receptor D 1.29 *** 1.28 NM_145826 Il17re interleukin 17 receptor E -1.5 **** -1.33 **** NM_008360 Il18 interleukin 18 1.7 **** -1.03 NM_010531 Il18bp interleukin 18 binding protein 7.71 **** 9.58 **** NM_008365 Il18r1 interleukin 18 receptor 1 13.81 **** 3.51 **** NM_010554 Il1a interleukin 1 alpha 1.32 1.76 *** NM_008361 Il1b interleukin 1 beta 1.74 *** 1.89 **** NM_010555 Il1r2 interleukin 1 receptor, type II 1.59 *** 1.28 *** NM_008364 Il1rap interleukin 1 receptor accessory protein 1.82 **** 1.23 *** NM_031167 Il1rn interleukin 1 receptor antagonist 1.48 *** 1.76 **** NM_001033543 Il20rb interleukin 20 receptor beta 1.49 *** 1 NM_021782 Il21 interleukin 21 2.27 *** 1.28 ** NM_145636 Il27 interleukin 27 1.62 *** 1.22 NM_177396 Il28b interleukin 28B 1.79 *** 1.5 *** NM_133775 Il33 interleukin 33 1.42 **** 1.28 NM_029646 Il34 interleukin 34 1.43 *** 1.27 ** NM_001008700 Il4ra interleukin 4 receptor, alpha 2 **** 1.65 *** NM_031168 Il6 interleukin 6 26.26 **** 14.77 **** NM_010559 Il6ra interleukin 6 receptor, alpha -1.91 **** -1.92 **** NM_008371 Il7 interleukin 7 2.6 **** 1.64 **** NM_013693 Tnf tumor necrosis factor 3.9 **** 1.28 NM_011693 Vcam1 vascular cell adhesion molecule 1 4.01 **** 2.14 **** Other innate immune response components NM_008198 Cfb complement factor B 59.1 **** 50.91 **** NM_172689 Ddx58 (RIG-I) DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 12.6 **** 4.34 **** NM_001081215 Ddx60 DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 16.11 **** 6.45 **** NM_007843 Defb1 defensin beta 1 4.03 **** 3.06 *** NM_032541 Hamp hepcidin antimicrobial peptide 57.48 **** 25.46 **** NM_008324 Ido1 indoleamine 2,3-dioxygenase 1 4.93 **** 5.74 **** NM_145949 Ido2 indoleamine 2,3-dioxygenase 2 1.33 *** -1.03 NM_027835 Ifih1 (MDA5) interferon induced with helicase C domain 1 4.29 **** 1.76 **** NM_008392 Irg1 immunoresponsive gene 1 46.53 **** 8.46 **** NM_010846 Mx1 myxovirus (influenza virus) resistance 1 7.16 **** 4.21 **** NM_145211 Oas1a 2'-5' oligoadenylate synthetase 1A 9.6 **** 4.98 **** NR_003507 Oas1b 2'-5' oligoadenylate synthetase 1B 7.12 **** 3.92 *** NM_033541 Oas1c 2'-5' oligoadenylate synthetase 1C 1.43 *** -1.21 * NM_145227 Oas2 2'-5' oligoadenylate synthetase 2 13.71 **** 11.9 **** NM_145226 Oas3 2'-5' oligoadenylate synthetase 3 2.47 **** 1.52 ** NM_145209 Oasl1 2'-5' oligoadenylate synthetase-like 1 3.92 **** 1.99 **** NM_011854 Oasl2 2'-5' oligoadenylate synthetase-like 2 34.6 **** 17.97 **** NM_054096 Tirap toll-interleukin 1 receptor (TIR) domain-containing adaptor protein 1.54 *** 1.03 NM_011905 Tlr2 toll-like receptor 2 10.37 **** 3.05 **** NM_126166 Tlr3 toll-like receptor 3 5.97 **** 1.62 **** Proteasome, antigen presentation NM_001042605 Cd74 CD74 antigen (invariant polypeptide of major histocompatibility 11.53 **** 33.07 **** complex, class II antigen-associated) NM_010381 H2-Ea histocompatibility 2, class II antigen E alpha 1.06 1.05 NM_013819 H2-M3 histocompatibility 2, M region locus 3 1.71 **** 1.62 **** NM_010398 H2-T23 histocompatibility 2, T region locus 23 5.12 **** 6.39 **** NM_145487 Prr3 proline-rich polypeptide 3 1.01-1.02

NM_010724 Psmb8 proteasome (prosome, macropain) subunit, beta type 8 (large 14.3 **** 14.37 **** multifunctional peptidase 7) NM_011190 Psme2 proteasome (prosome, macropain) 28 subunit, beta 5.16 **** 5.77 **** NM_009066 Ring1 ring finger protein 1-1.22 *** -1.08 * NM_008202 Slc39a7 solute carrier family 39 (zinc transporter), member 7-1.12-1.41 **** NM_013683 Tap1 (Mtp-1) transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) 25.02 **** 18.25 **** NM_011530 Tap2 (Mtp-2) transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) 8.41 **** 6.9 **** NM_172620 Vps52 vacuolar protein sorting 52 (yeast) -1.14 * -1.06 (there are also a large number of up-regulated HLA-related molecules) Hormones, growth factors NM_009627 Adm adrenomedullin 1.1 2.25 ** NM_007693 Chga chromogranin A -1.07-1.26 *** NM_007694 Chgb chromogranin B 1.17 * 1.17 NM_010197 Fgf1 fibroblast growth factor 1-1.57 **** -1.49 ** NM_011046 Furin furin (paired basic amino acid cleaving enzyme) -1.19-1.11 NM_008100 Gcg glucagon -1.02-1.23 NM_010491 Iapp islet amyloid polypeptide -1.01-1.05 NM_183029 Igf2bp2 insulin-like growth factor 2 mrna binding protein 2 1.64 **** 3.34 *** NM_008386 Ins1 insulin I 1.02-1.05 *** NM_008387 Ins2 insulin II -1.01-1.04 NM_013628 Pcsk1 (Pc1) proprotein convertase subtilisin/kexin type 1-1.09 * -2.57 **** NM_008792 Pcsk2 (Pc2) proprotein convertase subtilisin/kexin type 2-1.08 *** -1.34 **** NM_011057 Pdgfb platelet derived growth factor, B polypeptide 1.61 *** 1.52 NM_009215 Sst somatostatin -1.5 **** -3.51 * NM_011577 Tgfb1 transforming growth factor, beta 1 1.63 ** 1.6 ** NM_001025250 Vegfa vascular endothelial growth factor A -1.48 **** 1.16 *** NM_011697 Vegfb vascular endothelial growth factor B -1.45 *** -1.01 Hormone and growth factor receptors NM_008082 Galr1 galanin receptor 1-8.14 **** -3.81 **** NM_008101 Gcgr glucagon receptor -1.88 **** -1.32 ** NM_010284 Ghr growth hormone receptor -1.79 **** -1.45 **** NM_001080815 Gipr gastric inhibitory polypeptide receptor -2.37 **** -2.84 *** NM_021332 Glp1r glucagon-like peptide 1 receptor -3.22 **** -2.51 **** NM_010513 Igf1r insulin-like growth factor I receptor 2.04 **** 1.6 * NM_010515 Igf2r insulin-like growth factor 2 receptor 1.48 **** 1.06 NM_010568 Insr insulin receptor -1.4 **** 1.08 NM_010568 Insr insulin receptor -1.55 **** 1.02 NM_001081147 Oxtr oxytocin receptor -2.63 **** -3.14 **** NM_001146268 Pdgfrb platelet derived growth factor receptor, beta polypeptide -1.68 *** -1.57 NM_201615 Prlhr prolactin releasing hormone receptor -1.31 **** -1.14 * BC096586 Prlr prolactin receptor -2.6 **** -8.25 **** NM_011169 Prlr prolactin receptor -2.66 **** -4.86 *** NM_009370 Tgfbr1 transforming growth factor, beta receptor I -1.32 *** -1.29 NM_009504 Vdr vitamin D receptor -1.56 *** -2.25 *** Free radical scavenger, DNA damage response NM_009804 Cat catalase 1.23 * 2.88 **** NM_007631 Ccnd1 cyclin D1-1.61 **** -2.03 **** NM_023243 Ccnh cyclin H -1.46 **** -1.68 *** NM_007668 Cdk5 cyclin-dependent kinase 5-1.5 **** -1.23 *** NM_183294 Cdkl1 cyclin-dependent kinase-like 1 (CDC2-related kinase) -2.22 **** -2.07 ** NM_033569 Cnnm2 cyclin M2-1.35 *** -1.15 NM_008655 Gadd45b growth arrest and DNA-damage-inducible 45 beta 6.57 **** 13.11 **** NM_011817 Gadd45g growth arrest and DNA-damage-inducible 45 gamma 1.72 *** 1.85 **** NM_008160 Gpx1 glutathione peroxidase 1 1.83 **** 2.63 **** NM_030677 Gpx2 glutathione peroxidase 2-1.37 **** 1.06 NM_010344 Gsr glutathione reductase 1.95 **** 1.69 **** NM_001077353 Gsta3 glutathione S-transferase, alpha 3 1.09 1.99 **** NM_026672 Gstm7 glutathione S-transferase, mu 7-1.58 *** -1.42 *** NM_010362 Gsto1 glutathione S-transferase omega 1-1.17 ** 2.55 *** NM_010442 Hmox1 heme oxygenase (decycling) 1 2.95 **** 7 **** NM_013602 Mt1 metallothionein 1 3.67 **** 4.55 **** NM_008630 Mt2 metallothionein 2 7.27 **** 8.89 **** NM_172893 Parp12 poly (ADP-ribose) polymerase family, member 12 6.32 **** 4.93 **** NM_009632 Parp2 poly (ADP-ribose) polymerase family, member 2-1.12 *** -1.01

NM_145619 Parp3 poly (ADP-ribose) polymerase family, member 3 2.25 **** 1.82 **** NM_030253 Parp9 poly (ADP-ribose) polymerase family, member 9 17.91 **** 6.35 **** NM_178939 Pdrg1 p53 and DNA damage regulated 1 1.15 * 1.2 NM_013671 Sod2 (MnSOD) superoxide dismutase 2, mitochondrial 4.47 **** 2.65 **** NM_011660 Txn1 thioredoxin 1 1.29 *** -1.06 NM_001042523 Txnrd1 thioredoxin reductase 1 1.67 **** 1.41 *** Cell cycle NM_007631 Ccnd1 cyclin D1-1.61 **** -2.03 **** NM_023243 Ccnh cyclin H -1.46 **** -1.68 *** NM_007661 Cdk11b cyclin-dependent kinase 11B 1.01 1.17 **** NM_007668 Cdk5 cyclin-dependent kinase 5-1.5 **** -1.23 *** NM_033569 Cnnm2 cyclin M2-1.71 **** -1.54 **** NM_033569 Cnnm2 cyclin M2-1.35 *** -1.15 Endoplasmic reticulum stress, translational regulation NM_007498 Atf3 activating transcription factor 3 5.12 **** 6.89 **** NM_009716 Atf4 activating transcription factor 4 1.15 ** 1.01 NR_027838 Atp2a2 (Serca2b) ATPase, Ca++ transporting, cardiac muscle, slow twitch 2-1.78 **** -1.33 *** NM_009788 Calb1 calbindin 1-4.18 **** -4.05 **** NM_007837 Ddit3 (Chop) DNA-damage inducible transcript 3 2.11 *** 9.11 **** NM_024207 Derl1 Der1-like domain family, member 1 1.16 * 1.51 *** NM_138677 Edem1 ER degradation enhancer, mannosidase alpha-like 1 1.19 * 1.53 *** NM_007906 Eef1a2 eukaryotic translation elongation factor 1 alpha 2-1.35 *** -1.77 *** NM_010120 Eif1a eukaryotic translation initiation factor 1A 1.86 **** 1.04 * NM_001005509 Eif2a eukaryotic translation initiation factor 2a -1.02 1.26 NM_011163 Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 2 6.36 **** 1.56 **** NM_010121 Eif2ak3 (Perk) eukaryotic translation initiation factor 2 alpha kinase 3 1.3 *** 1.52 * NM_153403 Eif2c1 eukaryotic translation initiation factor 2C, 1-1.7 **** -1.33 *** NM_153402 Eif2c3 eukaryotic translation initiation factor 2C, 3-1.36 **** 1.06 NM_153177 Eif2c4 eukaryotic translation initiation factor 2C, 4-1.42 *** -1.08 NM_001033269 Eif4e1b eukaryotic translation initiation factor 4E family member 1B -1.47 *** -1.1 NM_007918 Eif4ebp1 eukaryotic translation initiation factor 4E binding protein 1 1.35 **** 1.35 * NM_198303 Eif5b eukaryotic translation initiation factor 5B 1.43 **** 1.56 **** NM_010579 Eif6 eukaryotic translation initiation factor 6 2.75 **** 1.98 **** NM_023913 Ern1 (IRE1) endoplasmic reticulum (ER) to nucleus signalling 1-1.43 *** 1.27 * AK078618 Hspa1b (Hsp70) heat shock protein 1B -1.1 1.08 NM_022310 Hspa5 (Bip) heat shock protein 5-1.02-1.42 **** NM_031165 Hspa8 heat shock protein 8 1.14 *** 1.43 **** NM_010481 Hspa9 heat shock protein 9 1.16 2.01 **** NM_175111 Hspbap1 Hspb associated protein 1 1.2 *** 1.38 **** NM_010477 Hspd1 heat shock protein 1 (chaperonin) -1.24 *** 1.24 *** NM_013559 Hsph1 heat shock 105kDa/110kDa protein 1-1.26 * 1.45 * NM_021395 Hyou1 (Orp150) hypoxia up-regulated 1-1.24 **** -1.39 **** NM_008549 Man2a1 mannosidase 2, alpha 1-1.37 *** -2.63 **** NM_001003913 Mars methionine-trna synthetase -1.03-1.12 * NM_008943 Psen1 presenilin 1 1.69 **** 1.3 **** NM_016906 Sec61a1 Sec61 alpha 1 subunit (S. cerevisiae) -1.1 *** -2.02 **** NM_021305 Sec61a2 Sec61, alpha subunit 2 (S. cerevisiae) -1.16 **** -1.24 Apoptosis related NM_026531 Aen apoptosis enhancing nuclease 1.58 **** 2.42 **** NM_001042558 Apaf1 apoptotic peptidase activating factor 1 2.15 **** 1.86 **** NM_009736 Bag1 BCL2-associated athanogene 1 (oncogene; increases Bcl2 act.) 1.4 **** 1.68 **** NM_145392 Bag2 BCL2-associated athanogene 2-1.43 ** -1.38 *** NM_013863 Bag3 BCL2-associated athanogene 3 1.8 **** 1.27 NM_007523 Bak1 BCL2-antagonist/killer 1 2.55 **** 1.61 **** NM_007527 Bax BCL2-associated X protein 1.06 1.78 **** NM_133234 Bbc3 (Puma) BCL2 binding component 3-1.01 1.15 * NM_009743 Bcl2l1 (Bcl-XL) BCL2-like 1 2.14 **** 2.1 **** NM_007544 Bid BH3 interacting domain death agonist 3.73 **** 2.47 **** NM_007546 Bik BCL2-interacting killer 1.37 *** -1.28 NM_009807 Casp1 caspase 1 8.25 **** 6.54 **** NM_009808 Casp12 caspase 12 1.93 *** 2.24 *** NM_007610 Casp2 caspase 2 2.18 **** 1.31 ** NM_009810 Casp3 caspase 3 3.8 **** 2.55 **** NM_007609 Casp4 caspase 4, apoptosis-related cysteine peptidase 17.33 **** 8.34 ****

NM_007611 Casp7 caspase 7 6.67 **** 5.27 **** NM_009812 Casp8 caspase 8 1.93 **** 1.11 * NM_207653 Cflar CASP8 and FADD-like apoptosis regulator 1.57 **** -1.52 *** NM_146057 Dap death-associated protein 1.39 *** 1.42 **** NM_029723 Dapl1 death associated protein-like 1-2.89 **** -3.66 **** NM_007829 Daxx (Fas Fas death domain-associated protein 2.64 **** 1.27 *** associated) NM_007987 Fas Fas (TNF receptor superfamily member 6) 23.43 **** 14.95 **** NM_007545 Hrk (Dp5) harakiri, BCL2 interacting protein (contains only BH3 domain) -1.06-1.15 NM_022323 Moap1 modulator of apoptosis 1 (obs interacts with Bax) 1.6 **** 1.06 * NM_011052 Pdcd6ip programmed cell death 6 interacting protein (obs apoptosis 1.32 **** 1.61 **** NM_008654 Ppp1r15a protein phosphatase 1, regulatory (inhibitor) subunit 15A 1.34 *** 3.64 **** NM_027154 Tmbim1 transmembrane BAX inhibitor motif containing 1 1.41 *** 1.68 **** NM_175093 Trib3 tribbles homolog 3 (Drosophila) 1.7 ** 4.67 **** ENSMUST000000 29662 Kinases, phosphatases Alpk1 alpha-kinase 1 6.02 **** 2.46 **** Alpk1 alpha-kinase 1 9.95 **** 2.39 **** Alpk1 alpha-kinase 1 4.25 **** 2.14 *** NM_013642 Dusp1 dual specificity phosphatase 1 (obs dusps phosphatases modulate 2.18 ** 2.26 * ERK/MAPKs) NM_022019 Dusp10 dual specificity phosphatase 10 2.47 **** 1.54 NM_130447 Dusp16 dual specificity phosphatase 16 2.59 **** 2.53 ** NM_173745 Dusp18 dual specificity phosphatase 18-2.39 **** -2.31 **** NM_024438 Dusp19 dual specificity phosphatase 19-1.9 **** -1.73 *** NM_028207 Dusp3 dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-1.33 **** 1.08 related) NM_026268 Dusp6 dual specificity phosphatase 6-2.45 *** -1.47 NM_008748 Dusp8 dual specificity phosphatase 8 1.82 **** 3.55 ** NM_145527 Madd MAP-kinase activating death domain -1.74 **** -1.53 *** NM_015806 Mapk6 mitogen-activated protein kinase 6 2.48 **** 1.66 * NM_008551 Mapkapk2 MAP kinase-activated protein kinase 2 2.39 **** 2.27 **** NM_011035 Pak1 p21 protein (Cdc42/Rac)-activated kinase 1 2.23 **** 1.82 **** NM_011103 Prkcd protein kinase C, delta 1.47 *** 1.49 **** NM_011201 Ptpn1 protein tyrosine phosphatase, non-receptor type 1 2.94 **** 1.56 **** NM_001081043 Ptpn23 protein tyrosine phosphatase, non-receptor type 23 1.45 *** 1.61 *** Others NM_007405 Adcy6 adenylate cyclase 6-1.65 **** -1.34 *** NM_007440 Alox12 arachidonate 12-lipoxygenase 5.02 **** 2.56 **** NM_007600 Capn1 calpain 1 1.38 **** 1.32 **** NM_008077 Gad1 (Gad67) glutamic acid decarboxylase 1-2.45 **** -1.39 * NM_001146275 Iigp1 interferon inducible GTPase 1 466.26 **** 145.01 **** NM_008875 Pld1 phospholipase D1 2.35 **** 1.62 *** NM_023137 Ubd ubiquitin D 14.55 **** 21.93 **** NM_145500 Ubtd1 ubiquitin domain containing 1 1.58 *** 1.19 ** NM_011671 Ucp2 uncoupling protein 2 (mitochondrial, proton carrier) -1.44 *** -2.15 ****

6 h (fold change) 24 h (fold change) Cyt 1.25+Cyt 1.25 Cyt 1.25+Cyt 1.25 Accession Symbol Gene name / functional group vs Con vs Cyt vs Con vs Con vs Cyt vs Con Cell cycle and cell growth NM_016746 Ccnc cyclin C -1.01-1.13 * -1,15 * -1.45 ** 1.3 *** -1.02 NM_023243 Ccnh cyclin H -1.46 **** -1.09 1,1 ** -1.68 *** 1.45 *** 1,23 * NM_007659 Cdk1 cyclin-dependent kinase 1-1.16-1.08 1.09-1.48 **** -1.57 *** -1.23 NM_172409 Fmnl2 formin-like 2 2.79 **** 1.15 ** 2,16 **** 1.51 *** 1.54 *** 2,06 **** NM_019988 Mlst8 MTOR associated protein, LST8 homolog (S. cerevisiae) -1.22 * -1.04-1.06-1.75 **** 1.48 *** 1.01 Cell death NM_029723 Dapl1 death associated protein-like 1-2.89 **** -1.18-1,71 *** -3.66 **** -2.69 *** -1.73 NM_026637 Ggct gamma-glutamyl cyclotransferase 2.25 **** -1.4 *** -1.07 1.54 ** -1.01 1.07 Cellular stress, DNA damage NM_009804 Cat catalase 1.23 * 1.36 *** 1,57 *** 2.88 **** 1.95 *** 1,79 *** NM_011817 Gadd45g growth arrest and DNA-damage-inducible 45 gamma 1.72 *** 1.01 1,13 *** 1.85 **** -1.45 *** 1.06 NM_010442 Hmox1 heme oxygenase (decycling) 1 2.95 **** 1.41 ** 1.02 7 **** 1.71 1.15 NM_008706 Nqo1 NAD(P)H dehydrogenase, quinone 1-1.3-1.12-1.07 2.62 **** -1.39 *** -1,16 * NF-κB signaling NM_019777 Ikbke (IKKε) inhibitor of kappab kinase epsilon 9.22 **** -1.1 1.13 2.99 **** -1.31 *** 1.05 NM_019408 Nfkb2 (p52) nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, p49/p100 10.76 **** -1.13 1.09 3.86 **** -1.81 **** -1.07 NM_010908 Nfkbib nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta 2.7 **** -1.07-1.06 1.95 **** -1.26 *** -1 (IκBβ) NM_008690 Nfkbie nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon 3.26 **** -1.39 **** 1.08 1.49 * -1.56 *** -1.08 (IκBε) NM_177073 Relt RELT tumor necrosis factor receptor -2.15 **** 1.42 *** 1,7 **** -1.54 **** 1.33 ** 1,6 **** NM_134131 Tnfaip8 tumor necrosis factor, alpha-induced protein 8 4.22 **** 1.03 1.01 1.42 ** 1.37 **** -1.03 NM_008764 Tnfrsf11b tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) 6.81 **** 1.8 ** -1.06 2.59 *** 2.37 **** -1.03 NM_011612 Tnfrsf9 tumor necrosis factor receptor superfamily, member 9 4.38 **** 1.02 1.23 3.05 **** -1.6 ** -1.1 NM_175093 Trib3 tribbles homolog 3 (Drosophila) 1.7 ** -1.08-1.12 4.67 **** 1.39 *** 1.09 Chemokines and cytokines NM_011333 Ccl2 chemokine (C-C motif) ligand 2 70.4 **** -1.55 *** 2.13 15.19 **** -1.96 ** -1.01 (MCP-1) NM_013653 Ccl5 chemokine (C-C motif) ligand 5 3.99 **** -1.67 *** 1,13 * 4.21 **** -1.6 1.04 (RANTES) NM_021443 Ccl8 chemokine (C-C motif) ligand 8 2.15 **** -1.44 * 1.04 3 **** -1.66 *** 1.04 (MCP-2) NM_008176 Cxcl1 chemokine (C-X-C motif) ligand 1 14.57 **** -1.4 *** 1.24 4.06 **** -1.35 *** 1 NM_019494 Cxcl11 chemokine (C-X-C motif) ligand 11 43.34 **** -1.4 *** 1.42 13.57 **** -3 **** 1.05

NM_023158 Cxcl16 chemokine (C-X-C motif) ligand 16 6.24 **** -1.3 *** -1.03 8.57 **** -1.4 * -1 NM_009140 Cxcl2 chemokine (C-X-C motif) ligand 2 23.18 **** -1.9 *** 1.1 2.78 * -1.25-1.17 NM_008599 Cxcl9 chemokine (C-X-C motif) ligand 9 203.3 **** -1.17 2.2 191.01 **** -1.74 * 1 NM_008357 Il15 interleukin 15 2.55 **** -1.42 *** -1,1 *** 1.64 ** -1.31 * 1.01 NM_008360 Il18 interleukin 18 1.7 **** -1.44 *** -1.04-1.03 1.02-1.04 NM_008365 Il18r1 interleukin 18 receptor 1 13.81 **** 1.9 **** 1.04 3.51 **** 2.1 * 1.05 NM_133193 Il1rl2 interleukin 1 receptor-like 2 1.11-1.11-1.08 1.47 *** -1.45 *** 1.16 NM_010215 Il4i1 interleukin 4 induced 1 19.66 **** -1.4 **** 1.4 6.12 **** -1.72 **** -1,17 ** NM_031168 Il6 interleukin 6 26.26 **** -1.49 * 1.16 14.77 **** -1.56 1.02 NM_008371 Il7 interleukin 7 2.6 **** 1.46 *** 1.11 1.64 **** 2.09 **** 1.06 Other immune response components NM_178592 Bat5 HLA-B associated transcript 5 1.7 **** 1.01-1.03 1.84 *** -1.32 ** 1.04 NM_144938 C1s complement component 1, s subcomponent 11.63 **** -1.49 ** 1.03 19.77 **** -1.19-1.04 NM_009778 C3 complement component 3 3.76 **** 2.29 **** 1.01 10.61 **** 3.27 *** 1.08 NM_001039185 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 6.3 **** 1.54 ** 2,43 *** 2.98 **** 1.06 1,55 *** NM_007695 Chi3l1 chitinase 3-like 1 3.35 **** 2.36 **** 1.23 11.14 **** 5.12 **** 1.07 (Ykl-40) NM_130449 Colec12 collectin sub-family member 12-1.1 * 1.09 * -1.02 1.36 ** -1.45 **** 1.17 NM_007843 Defb1 defensin beta 1 4.03 **** 1.03 1,41 ** 3.06 *** 1.26 * 1.26 NM_032541 Hamp hepcidin antimicrobial peptide 57.48 **** -1.01 1.21 25.46 **** -1.63 * -1.1 NM_010493 Icam1 intercellular adhesion molecule 1 30.38 **** -1.15 * 1.49 11.77 **** -1.3 * -1.27 NM_008329 Ifi204 interferon activated gene 204 7.2 **** -1.33 1.04 4.05 **** -1.39 ** -1.08 (Ifi16) NM_001146275 Iigp1 interferon inducible GTPase 1 466.26 **** -1.11 2.81 145.01 **** 1.34 *** 1.02 NM_008392 Irg1 immunoresponsive gene 1 46.53 **** 1.27 *** 1.39 8.46 **** 1.32-1,15 *** NM_008326 Irgm1 immunity-related GTPase family M member 1 31.07 **** -1.03 1.31 15.32 **** 1.44 ** -1.08 NM_008522 Ltf lactotransferrin 8.3 **** 1.54 ** 1.2 11.92 **** 1.51 *** -1.02 NM_010846 Mx1 myxovirus (influenza virus) resistance 1 7.16 **** -1.59 **** 1,18 * 4.21 **** -1.21 1,21 ** NR_003508 Mx2 myxovirus (influenza virus) resistance 2 9.93 **** -1.21 1.2 8.56 **** -1.59 *** 1.1 NM_145227 Oas2 2'-5' oligoadenylate synthetase 2 13.71 **** -1.12 1.41 11.9 **** -1.64 * -1.21 NM_145209 Oasl1 2'-5' oligoadenylate synthetase-like 1 3.92 **** -1.08 1.04 1.99 **** -1.32 *** 1.03 NM_008768 Orm1 orosomucoid 1 1.26-1.04 1.07 2.23 ** 2.1 *** 1.03 NM_018851 Samhd1 SAM domain and HD domain, 1 6.27 **** 1.63 **** -1.06 3.01 **** 1.34 *** 1,08 * NM_011905 Tlr2 toll-like receptor 2 10.37 **** -1.13 1.04 3.05 **** 1.53 **** -1.06 NM_126166 Tlr3 toll-like receptor 3 5.97 **** -1.06-1,1 ** 1.62 **** 1.32 * -1.07 NM_009396 Tnfaip2 tumor necrosis factor, alpha-induced protein 2 20.36 **** -1.59 *** 1.21 5.76 **** -1.39 * -1.01 NM_011693 Vcam1 vascular cell adhesion molecule 1 4.01 **** -1.36 1.03 2.14 **** -1.39 **** 1.04 Ceramide, sphingomyelin, ganglioside NM_001004150 A4galt alpha 1,4-galactosyltransferase 1.57 *** 1.33 *** 1,19 * 1.94 *** -1.06 1.2 NM_133888 Smpdl3b sphingomyelin phosphodiesterase, acid-like 3B 2.25 **** -1.42 *** -1.04 1.64 ** -1.16 1.05 NM_011375 St3gal5 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 1.27 **** 1.49 *** 2,33 **** -1.74 **** 1.77 *** 1,73 ***

NM_011374 St8sia1 ST8 alpha-n-acetyl-neuraminide alpha-2,8-sialyltransferase 1-3.77 **** 1.57 **** 1,54 **** -2.03 **** 1.87 **** 1.52 Cell-to-cell contact, extracellular matrix NM_007403 Adam8 a disintegrin and metallopeptidase domain 8 1.45 *** -1.05-1,06 * 1.78 *** -1.35 ** -1 NM_172845 Adamts4 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 2.04 *** -1.13 1.16 1.71 *** -1.5 *** -1.01 NM_001081020 Adamts6 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 6 1.32 **** -1.43 ** -1.02 1.04-1.01 1.07 NM_178114 Amigo2 adhesion molecule with Ig like domain 2 1.21 2.69 **** 2,69 **** 2.15 *** 3.09 **** 2,41 **** NM_172862 Frem2 Fras1 related extracellular matrix protein 2-2.85 **** 1.61 **** 1.19-1.79 ** -1.07 1,25 * NM_008397 Itga6 integrin alpha 6 1.33-1.02-1.11-2.01 **** 1.36 *** -1.06 NM_178665 Lpp LIM domain containing preferred translocation partner in lipoma 1.53 *** -1.08 1.02 1.44 *** -1.31 **** 1.08 NM_008607 Mmp13 matrix metallopeptidase 13 2.65 **** 4.01 **** 1,58 * 5.92 **** 3.95 **** 3,45 *** NM_001081287 Mpp7 membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) -1.42 ** 2.37 **** 2,15 **** -1.87 **** 2.81 **** 1,78 **** NM_001111324 Nedd9 neural precursor cell expressed, developmentally down-regulated gene 9 1.16 2.26 **** 1,95 **** 1.86 **** 2.41 **** 1,59 *** NM_053145 Pcdhb20 protocadherin beta 20-1.18 * -1.04-1.01-1.98 **** 1.51 *** -1.12 NM_008873 Plau plasminogen activator, urokinase 1.26-1.14-1.1 1.71 *** -1.44 ** 1.07 NM_009369 Tgfbi transforming growth factor, beta induced 1.27 ** -1.05-1.1 1.52 **** -1.36 *** -1.06 Exocytosis, intracellular trafficking, cytoskeleton NM_175164 Arhgap26 Rho GTPase activating protein 26 2.27 **** -1.1 1.09 1.67 **** -1.49 **** 1.04 NM_019665 Arl6 ADP-ribosylation factor-like 6-1.28 *** 1.04-1.08-1.42 **** 1.32 *** -1.05 NM_009748 Bet1 blocked early in transport 1 homolog (S. cerevisiae) -1.38 **** -1.13 * -1,11 * -3.9 **** 1.51 *** 1 NM_007599 Capg capping protein (actin filament), gelsolin-like 1.29-1.12 1.02 1.64 *** -1.42 **** 1.04 NM_001081276 Clasp1 CLIP associating protein 1-1.05 1.2 * 1.03 1.31 **** 1.74 **** -1.01 NM_029793 Golga1 golgi autoantigen, golgin subfamily a, 1-1.3 **** -1.03-1.02-2.18 *** 1.45 *** 1.08 NM_175193 Golim4 golgi integral membrane protein 4-2.32 **** -1.17-1,14 ** -1.54 *** 1.33 ** -1.06 NM_146120 Gsn gelsolin -1.28 *** -1.04-1.08 1.42 *** -1.39 *** -1.07 NM_178920 Mal2 mal, T-cell differentiation protein 2 3.34 **** 1.08-1.1 1.58 *** 1.34 ** -1,15 * NM_153789 Mylip myosin regulatory light chain interacting protein 1.48 *** -1.41 **** -1,17 ** 1.62 **** -1.28 *** -1.02 NM_001033210 Pls1 plastin 1 (I-isoform) -1.62 **** -1.08 1,1 *** -1.91 **** 1.33 *** 1.06 NM_013831 Pstpip2 proline-serine-threonine phosphatase-interacting protein 2 1.32 ** -1.31 ** -1.07-1.22-1.26 * -1.09 NM_023537 Rab3b RAB3B, member RAS oncogene family -1.19-1.1-1.17 1.54 *** -1.53 *** -1.12 NM_176971 Rab9b RAB9B, member RAS oncogene family -1.84 **** 1.42 **** 1,8 **** -1.99 **** 1.44 *** 1.2 NM_028810 Rnd3 Rho family GTPase 3 7.11 **** -1.33 *** -1,18 *** 4.42 *** -1.05-1.16 NM_172926 Snx14 sorting nexin 14-1.57 **** -1.04-1,13 *** -2.23 *** 1.34 ** -1.02 NM_175483 Snx33 sorting nexin 33 1.07-1.02-1.08 1.33 *** 1.31 *** 1,18 ** NM_029655 Snx7 sorting nexin 7 1.35 *** -1.31 *** -1.09 1.34 * -1.19 ** -1.08 BC118516 Stx11 syntaxin 11 6.22 **** -1.53 **** 1.01 2.03 **** -1.3 *** -1.11 NM_016797 Stx7 syntaxin 7 1.55 **** 1.34 **** 1.12 1.36 *** 1.31 **** 1.15 NM_144552 Stxbp6 syntaxin binding protein 6 (amisyn) -1.85 **** -1.4 *** -1,31 *** -2.06 **** 1.04-1,2 *** NM_011523 Synj2 synaptojanin 2-1.46 *** 1.83 **** 2,05 **** -1.42 * 1.77 *** 1,84 **** NM_020626 Tmem27 transmembrane protein 27-1.23 **** 1.23 **** 1,13 *** -2.03 **** 1.69 **** 1,35 **** NM_172406 Trak2 trafficking protein, kinesin binding 2-1.11 ** 1.13 1,46 *** -1.35 *** 1.39 *** 1,34 ****

Hormone and growth factor signaling NM_207655 Egfr epidermal growth factor receptor 1.64 **** 1.82 **** 1,15 *** 2.48 **** 1.2 1.16 NM_007950 Ereg epiregulin 2.33 **** -1.09-1.04 5.61 **** -2.06 *** -1.03 NM_008008 Fgf7 fibroblast growth factor 7 1.34 * -1.25-1.1 2.58 **** -1.71 *** -1,25 * NM_001081212 Irs2 insulin receptor substrate 2-1.19 *** 1.04 1.06 1.36 *** -1.57 *** 1.08 NM_001122733 Kit kit oncogene -1.4 * 1.01-1,31 * 1.53 *** -1.35 ** -1.16 NM_001085376 Pappa2 pappalysin 2-1.71 *** 1-1.19-2.8 *** -1.39 *** -2.01 NM_001085376 Pappa2 pappalysin 2-1.8 **** -1.07-1,28 *** -3.02 *** -1.43 ** -2.02 NM_008808 Pdgfa platelet derived growth factor, alpha -1.37 **** 1.48 **** 1,52 **** 1.26 * 1.3 ** 1,46 **** NM_011057 Pdgfb platelet derived growth factor, B polypeptide 1.61 *** 1.53 **** -1.01 1.52 1.46-1.15 NM_001145835 Ralgds ral guanine nucleotide dissociation stimulator 3.2 **** 1.33 *** 1,52 *** 1.41 ** 1.09 1,2 **** NM_009511 Vipr2 (VPAC2) vasoactive intestinal peptide receptor 2 1.09 1.13 1,96 *** 1.92 **** 1.48 **** 1,51 * Transcription factors etc. NM_172790 Ankrd52 ankyrin repeat domain 52-1.18 * -1.04-1.01-1.4 *** 1.33 *** 1.13 NM_028085 Anks4b ankyrin repeat and sterile alpha motif domain containing 4B -1.31 * 1.04-1.01-1.62 *** 1.35 *** -1.07 NM_010800 Bhlha15 basic helix-loop-helix family, member a15-1.14 1.13 * -1.1-3.99 **** 1.46 *** 1.15 NM_007865 Dll1 delta-like 1 (Drosophila) 3.99 **** -1.39 ** -1.04 1.98 *** -1.36 * -1,14 * NM_008235 Hes1 hairy and enhancer of split 1 (Drosophila) -1.39 *** 1.12-1,19 * 1.6 ** -1.41 ** -1.01 NM_010496 Id2 inhibitor of DNA binding 2 2.11 **** -1.34 *** -1,26 *** 2.01 *** -1.22-1.17 NM_021459 Isl1 ISL1 transcription factor, LIM/homeodomain -1.53 **** -1.04-1.06-3.63 **** 1.3 * -1.03 NM_013584 Lifr leukemia inhibitory factor receptor -1.54 ** 1.26 * 1,35 * -1.67 **** 1.39 *** 1,19 * NM_057173 Lmo1 LIM domain only 1 2.14 *** -1.16-1.07 1.61 *** -1.39 *** 1.06 NM_010849 Myc myelocytomatosis oncogene 1.54 *** -1.31 *** -1,33 * 4.74 *** 1.04-1 NM_010894 Neurod1 neurogenic differentiation 1-1.12 1.22 *** 1.04-2.15 *** 1.43 *** 1,09 *** NM_145434 Nr1d1 nuclear receptor subfamily 1, group D, member 1-1.14-1.01-1.13 6.04 **** -1.54 ** -1.22 NM_173440 Nrip1 nuclear receptor interacting protein 1 1.11 * 1.36 ** 1,2 **** 1.88 *** 1.31 ** 1,35 *** NM_013627 Pax6 paired box gene 6-1.35 **** 1.06 1.1-2.41 **** 1.31 *** -1.02 NM_011415 Snai2 snail homolog 2 (Drosophila) 1.09-1.06 1,66 *** 1.81 ** 1.52 **** 1,4 * NM_146236 Tceal1 transcription elongation factor A (SII)-like 1-2.15 **** -1.08-1,21 * -2.75 **** 1.34 ** -1.04 NM_007855 Twist2 twist homolog 2 (Drosophila) 1.24 * 1.03 1.14 1.33 *** -1.33 ** 1,07 * Glucose metabolism NM_001081326 Agl amylo-1,6-glucosidase, 4-alpha-glucanotransferase -2.11 **** 1.17 **** 1,07 *** -1.87 **** 1.48 *** 1.09 NM_021331 G6pc2 glucose-6-phosphatase, catalytic, 2-1.21 **** 1.22 **** 1.03-3.31 **** 1.54 **** 1,12 * NM_010292 Gck glucokinase 1.05 1.23 ** 1.04-1.46 *** 1.32 *** 1.07 NM_010497 Idh1 isocitrate dehydrogenase 1 (NADP+), soluble -1.49 **** 1.37 **** 1,26 * -2.42 **** 1.78 *** 1.05 NM_010699 Ldha lactate dehydrogenase A 1.11-1.01-1.06 1.94 ** -1.41 ** 1.09 NM_029760 Nubpl nucleotide binding protein-like -1.08-1.15-1,15 *** -1.32 ** 1.43 ** 1.02 NM_025399 Nudt14 nudix (nucleoside diphosphate linked moiety X)-type motif 14-1.26 **** -1.04 1.03-1.64 **** 1.3 *** 1.08 NM_008810 Pdha1 pyruvate dehydrogenase E1 alpha 1-1.92 *** 1.3 *** 1.02-3.46 **** 1.83 **** 1,25 ***

NM_133232 Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 9.8 **** -1.5 **** 1.14 4.08 **** -1.08 1.09 NM_175013 Pgm5 phosphoglucomutase 5 1.4 *** 2.01 ** 1,94 **** -1.27 1.03 1.12 Lipid and cholesterol metabolism NM_013454 Abca1 ATP-binding cassette, sub-family A (ABC1), member 1-1.49 **** -1.32 ** -1,31 **** 1.53 **** -1.38 *** -1.06 NM_009593 Abcg1 ATP-binding cassette, sub-family G (WHITE), member 1 1.27 *** -1.29 ** -1,29 **** 2.8 **** -1.38 **** -1.04 NM_207625 Acsl4 acyl-coa synthetase long-chain family member 4 1.69 **** 1.16 * -1.03 1.66 *** 1.47 **** -1.01 NM_009996 Cyp24a1 cytochrome P450, family 24, subfamily a, polypeptide 1 2.74 **** 56.2 **** 98,19 **** -1.05 119.02 **** 88,95 **** NM_010046 Dgat1 diacylglycerol O-acyltransferase 1-1.2 * 1.01-1.21 1.41 **** 1.32 *** 1 NM_026384 Dgat2 diacylglycerol O-acyltransferase 2 1.11 1.39 **** 1,34 **** 3.48 **** 3.1 **** 1,58 * NM_053272 Dhcr24 24-dehydrocholesterol reductase -1.59 **** 1.19 ** 1.14-1.98 **** 1.34 *** -1.04 NM_175512 Dhrs9 dehydrogenase/reductase (SDR family) member 9 3.87 **** 1.4 **** 1,56 **** 1.24 1.64 1.24 NM_026143 Far1 fatty acyl CoA reductase 1-1.08 *** 1.01 1.13-2.3 **** 1.37 *** 1,2 *** NM_178772 Nceh1 arylacetamide deacetylase-like 1-1.04 1.23 ** 1.02-1.42 **** 1.49 **** 1,29 *** NM_025972 Naaa N-acylethanolamine acid amidase 1.13 *** 1.7 **** 1.21 1.86 *** 1.66 **** 1.01 NM_008822 Pex7 peroxisomal biogenesis factor 7-1.74 *** -1.13-1.11-1.99 **** 1.37 ** 1.02 NM_013737 Pla2g7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) -1.04 1.06 1.09 1.8 *** -1.43 *** 1,8 *** NM_153133 Rdh9 retinol dehydrogenase 9 2.33 *** -1.03 1.01 1.9 ** -1.35 ** -1.05 Metabolism of amino acids and nucleic acids NM_026512 Bphl biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) -2.14 **** 1.18 1,7 **** -1.66 ** 1.64 *** 1,55 **** NM_030703 Cpn1 carboxypeptidase N, polypeptide 1-1.52 *** 1.04 1.02-2.33 **** 1.46 **** 1.06 NM_178676 Entpd3 ectonucleoside triphosphate diphosphohydrolase 3 1.31 *** 1.31 *** 1,34 *** 1.42 *** 1.41 *** 1,37 *** NM_173733 Suox sulfite oxidase -1.72 *** 1.03 1.08-2.74 **** 1.53 ** 1.07 NM_009477 Upp1 uridine phosphorylase 1 4.78 **** -1.05-1.15 2.1 **** -1.44 ** -1.09 Inositol phosphate metabolism NM_018864 Impa1 inositol (myo)-1(or 4)-monophosphatase 1-1.13 1.18 ** 1,36 **** -1.42 ** 1.67 *** 1,38 **** NM_010567 Inppl1 inositol polyphosphate phosphatase-like 1-1.68 **** 1.81 **** 1,54 **** -1.21 *** 1.26 *** 1,24 *** Metabolism of drugs etc. NM_007409 Adh1 alcohol dehydrogenase 1 (class I) -3.9 **** 1.8 **** 1,74 **** -2.65 **** 2.83 **** 1,57 ** NM_172532 Aldh5a1 aldhehyde dehydrogenase family 5, subfamily A1-1.38 *** 1.31 *** 1.25 1.13 1.08 1.17 NM_024495 Car13 carbonic anhydrase 13 11.43 **** -1.12 1.03 6.77 **** -1.47 *** 1.19 NM_007592 Car8 carbonic anhydrase 8 1.56 *** -1.33 ** -1,31 * -1.38 ** -1.58 *** -1.37 NM_026935 Sult1c2 sulfotransferase family, cytosolic, 1C, member 2-2.5 **** 2.06 **** 2,28 **** -1.22 3.18 **** 1,9 *** NM_153598 Ugt2b34 UDP glucuronosyltransferase 2 family, polypeptide B34-1.52 *** 1.17-1.03 1.83 **** 1.9 **** 1.07 Splicing machinery, RNA modification NR_004429 Adarb1 adenosine deaminase, RNA-specific, B1-1.54 **** 1.44 *** 1,15 ** -1.65 *** 1.67 **** 1.19 NM_145529 Cstf3 cleavage stimulation factor, 3' pre-rna, subunit 3-1.04-1.38 * -1.04-1.57 **** 1.33 *** 1,23 * NM_010413 Hdac6 histone deacetylase 6-1.97 **** 1.34 **** -1.12-3.79 **** 1.68 *** -1,1 ** NM_178632 Ints7 integrator complex subunit 7-1.33 **** -1.08-1.03-1.54 *** 1.31 *** -1 NM_008671 Nap1l2 nucleosome assembly protein 1-like 2-1.11-1.1-1.05-1.84 **** 1.31 *** -1,26 *** NM_013830 Prpf4b PRP4 pre-mrna processing factor 4 homolog B (yeast) -1.31 *** 1.37 *** 1,34 *** -1.14 1.37 **** 1,29 ***

NM_001081362 Trrap transformation/transcription domain-associated protein -1.43 * -1.09-1.01-1.77 **** 1.35 *** -1 Transport and homeostasis of ions etc. NM_013851 Abca8b ATP-binding cassette, sub-family A (ABC1), member 8b 2.06 **** -1.09-1,05 ** 1.95 **** 1.38 ** -1.07 NM_013806 Abcc2 ATP-binding cassette, sub-family C (CFTR/MRP), member 2-1.34 ** 1 1.06-1.55 **** 1.4 *** 1.11 NM_001007583 Best3 bestrophin 3-2.56 **** 1.24 **** 1,12 *** -2.28 *** 1.42 * 1,44 **** NM_001037712 Kcnh6 potassium voltage-gated channel, subfamily H (eag-related), member 6-2.7 **** 1.32 *** 1,64 **** -2.88 **** 1.66 *** 1,49 **** NM_175002 Mmgt2 membrane magnesium transporter 2-1.17 * 1.46 **** 1,6 **** -1.65 *** 1.74 *** 1,51 *** NM_011026 P2rx4 purinergic receptor P2X, ligand-gated ion channel 4-1.16 **** 1.32 **** 1,91 **** -1.99 *** 1.64 ** 1,54 **** NM_018852 Scn9a sodium channel, voltage-gated, type IX, alpha -2.24 **** -1.32 *** -1.27-2.33 *** 1.4 ** -1.07 NM_018852 Scn9a sodium channel, voltage-gated, type IX, alpha -1.98 **** -1.33 ** -1.22-2 **** 1.12-1.01 NM_011326 Scnn1g sodium channel, nonvoltage-gated 1 gamma 2.57 *** -1.34 **** -1.24 1.2-1.1-1,22 * NM_001161845 Sgk1 serum/glucocorticoid regulated kinase 1-1.93 **** 1.02-1,41 **** -1.32 ** -1.32 *** -1.11 NM_172838 Slc16a12 solute carrier family 16 (monocarboxylic acid transporters), member 12-2.16 **** 1.6 *** 1.05-2.64 **** 1.8 **** 1.05 NM_001033167 Slc22a23 solute carrier family 22, member 23-1.33 **** 1.51 **** -1-1.14 1.49 **** 1.07 NM_028048 Slc25a35 solute carrier family 25, member 35-1.02 1.05-1.16-1.91 **** 1.63 **** -1.08 NM_025286 Slc31a2 solute carrier family 31, member 2 1.69 **** 1.02 1,8 *** -1.33 ** 1.41 *** 1,56 **** NM_015728 Slc33a1 solute carrier family 33 (acetyl-coa transporter), member 1-1.14 **** -1.07 * -1,08 * -2.36 **** 1.35 *** 1.03 NM_028756 Slc35a5 solute carrier family 35, member A5-1.35 * -1.09-1.04-1.72 *** 1.32 ** -1.05 NM_029529 Slc35d3 solute carrier family 35, member D3-2.25 **** -1.07 * -1.07 2.32 **** 1.33 **** 1.1 NM_028060 Slc35f2 solute carrier family 35, member F2 2.11 **** 2.82 **** 1,25 *** 1.51 **** 1.02 1.12 NM_011406 Slc8a1 solute carrier family 8 (sodium/calcium exchanger), member 1-1.54 **** -1.13-1.12-1.8 **** 1.35 ** 1.07 NM_023718 Slco1a6 solute carrier organic anion transporter family, member 1a6-1.26 *** 1.56 **** 1,47 **** -2.58 *** 2.18 *** 1,72 **** NM_009285 Stc1 stanniocalcin 1 1.57 ** 1.54 ** 1.17 1.41 **** 1.12 1,03 * NM_027399 Steap1 six transmembrane epithelial antigen of the prostate 1 3.44 **** 1-1,41 *** 2.61 **** 1.41 ** 1.04 NM_054098 Steap4 STEAP family member 4 151.69 **** -1.04 2.15 12.6 **** 2.02 *** -1.06 Intracellular signaling NM_198111 Akap6 A kinase (PRKA) anchor protein 6-1.44 *** -1.52 *** -1,33 *** -1.22-1.39-1.26 NM_145429 Arrb2 arrestin, beta 2-1.82 **** 1.35 *** 1,76 **** -1.39 *** 1.63 *** 1,86 *** NM_023048 Asb4 ankyrin repeat and SOCS box-containing 4 14.5 **** -1.44 *** 1.09 3.14 **** -2.79 **** -1.02 NM_001081047 Cnksr1 connector enhancer of kinase suppressor of Ras 1 3.42 **** -1.15 * -1.11 3.44 **** -1.39 *** 1.07 NM_028785 Dock8 dedicator of cytokinesis 8-1.89 **** 1.15 * 1,14 * -2.03 **** 1.77 *** 1.12 NM_194057 Ffar1 free fatty acid receptor 1-2.21 **** -1.41 **** -1,3 ** -4.88 **** -1.17-1.11 NM_008137 Gna14 guanine nucleotide binding protein, alpha 14 5.68 **** 1.24 ** 1,6 *** 2.56 **** 1.38 ** 1.55 NM_018869 Grk5 G protein-coupled receptor kinase 5-1.39 **** 1.45 *** 1,29 *** -1.59 *** 1.66 **** 1,29 * NM_025768 Grtp1 GH regulated TBC protein 1-2.29 **** -1.54 **** -1,25 **** -1.92 **** -1.19-1.11 NM_008624 Mras muscle and microspikes RAS 1.25 * 1.01 1.09-1.36 *** 1.4 *** -1.07 NM_011055 Pde3b phosphodiesterase 3B, cgmp-inhibited -1.54 **** -1.01 1,46 *** -1.47 *** 1.44 **** 1,21 **** NM_001134480 Plcxd2 phosphatidylinositol-specific phospholipase C, X domain containing 2-1.19 *** -1.06 1.01 1.95 **** -1.39 *** 1,21 *** NM_029182 Rasd2 RASD family, member 2 1.48 *** 1.31-1.05 6.99 **** 1.39 ** -1 NM_011519 Sdc1 syndecan 1 1.56 **** 1.76 **** 1.06 1.6 *** 1.07-1.04 NM_011521 Sdc4 syndecan 4 3.3 **** -1.29 *** -1.1 2.08 **** -1.53 **** -1,18 *