Application of bone marrow mesenchymal stem cells in hematopoietic stem cell transplantation for sensitized and non-sensitized BALB/c mice

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1 9 8 Chinese Journal of Tissue Engineering Research August, Vol.9, No. BALB/c ( ) BALB/c / () BALB/c BALB/c BALB/c BALB/c 8 h BALB/c BALB/c d d BALB/c d / 97 :R9. :A :9- () BALB/c [J]. 9():-9. doi:.99/j.issn Application of bone marrow mesenchymal stem cells in hematopoietic stem cell transplantation for sensitized and non-sensitized BALB/c mice Guo Xiao-wei, Guan Chen-xia ( Orthopedics Hospital of Zhengzhou, Zhengzhou, Henan Province, China; Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China) Abstract BACKGROUND: Hematopoietic stem cell transplantation has therapeutic effects on many diseases, but its application has some limitations, such as cell harvesting and age-limited number of cells. OBJECTIVE: To investigate the application value of bone marrow mesenchymal stem cells in hematopoietic stem cell transplantation for sensitized and non-sensitized BALB/c mice. METHODS: Bone marrow cells derived from BALB/c mice were isolated and cultured in vitro to harvest mesenchymal stem cells using adherent method. The cell surface markers were detected by flow cytometry. A murine model of sensitization was established by transfusion of allogeneic spleen cells. Mesenchymal stem cells labeled with green fluorescent dye were transplanted into non-sensitized and sensitized recipient mice, and the homing of mesenchymal stem cells in vivo was monitored at different time points post transplantation. Additionally, under irradiation pretreatment, sensitized BALB/c mice under irradiation were subjected to combined transplantation of allogeneic bone marrow cells and syngeneic mesenchymal stem cells. Survival rate of BALB/c mice was monitored daily. RESULTS AND CONCLUSION: At 8 hours after transplantation, mesenchymal stem cells in sensitized and non-sensitized recipients were homing to the spleen and bone marrow, respectively. In the experiment of hematopoietic stem cell transplantation, the sensitized recipients died at days after combined Guo Xiao-wei, Master, Associate chief physician, Orthopedics Hospital of Zhengzhou, Zhengzhou, Henan Province, China Accepted: -- ISSN 9- CN -8/R CODEN: ZLKHAH

2 . BALB/c transplantation, with a median of days; however, the sensitized recipients only undergoing allogeneic bone marrow cell transplantation had a survival median of days. These findings indicate that the transplanted mesenchymal stem cells in sensitized recipients are mainly homing to the spleen and bone marrow, but the combination transplantation cannot enhance the transplantation of allogeneic hematopoietic stem/progenitor cells in sensitized recipients. Subject headings: Immunization; Hematopoietic Stem Cell Transplantation; Spleen; Cell Transplantation Funding: the Medical Science Key Project of Henan Province in, No. Guo XW, Guan CX. Application of bone marrow mesenchymal stem cells in hematopoietic stem cell transplantation for sensitized and non-sensitized BALB/c mice. Zhongguo Zuzhi Gongcheng Yanjiu. ;9():-9. Introduction [-] B T [-] / [-9] / [] BALB/c Materials and methods SPF BALB/c8C7BL/8 9 g SYXK( )- RPMI-DMEM/F Invitrogen Anti-CD-PE Anti-CD-PE Anti-Sca- BD PharMingen -FITC Anti-CD9-PE Anti-CDb-FITC Anti-CD-FITC Co 8 BALB/c DMEM/F ml ml Hanks r/min min cm.. ml h.. h ml d r/min min / ml cm ( ) CD CD Sca- CD9 CDlb CD [] C7BL/ BALB/c 7 d d BALB/c mg/l U/L h BALB/c8 Gy Co ( Gy+ 8 Gy) µmol/l PBS ( ) L 7 DMEM/F % min min BALB/c P.O. Box, Shenyang 8

3 . BALB/c A B C (%) (%) (%) Figure The homing of mesenchymal stem cells in mice A h B h C 8 h BALB/c BALB/c 8 h Tris-NH Cl BALB/c ( ) C7BL/ C7BL// BALB/c BALB/c RPM- L BALB/c 7 BALB/c BALB/c 7 C7BL/BALB/c BALB/c SPSS 7. t α=. Results. ( ) Scal + CD9 + CD + CD + CDb CD ( ) Figure Morphology of passage bone marrow mesenchymal stem cells ( ). hbalb/c hbalb/c 8 h BALB/c BALB/c h. BALB/c h BALB/c 8 Gy Co 8 Gy Co 7 8 d 7 BALB/c8 Gy Co 7 d d 8 Gy Co 7 d d BALB/c (P >.) / ISSN 9- CN -8/R CODEN: ZLKHAH 7

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