J OU RNAL OF THE CHIN ESE RARE EAR TH SOCIET Y , ; 2. : (2004) d, mol L - 1 EuCl 3, mol L (CL SM)

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1 22 1 Vol J OU RNAL OF THE CHIN ESE RARE EAR TH SOCIET Y Feb Ξ 1, 1 1, 3 2, (1., ; 2.,, ,, ) :,,,,,,, : ; ; : R77. 5 ; O614 : A : (2004) [ ], LaCl 3, 90 d,, ( 1) [1 ], mol L - 1 EuCl 3,,, mol L - 1 2, 1,,,,, ( ), ( ), (CL SM), ( t a ) c mol L - 1, 1 LaCl 3 90 d La Ξ : ; : : ( ) : ( ),,, ; : 3 ( E2mail : bjmu. edu. cn)

2 2 22, Tb mol L - 1,, CL SM Tb 3 +, Tb %, L n (cit) (cit ) Tb c 2Tf Tb % ; Tb 3 +, (apo2tf), Tb2Cit, Tb c Tf Tb2Asp, Tb2HSA ( Tf R), Tb mol (ionop hore), Na + 2La 3 + L - 1 Tb 3 + ( [ 9 ] 2),, [12,, ], > 5, ( FPL C) 3 [9 ] 2,, ( ),, 1000 [ Gd, (D TPA ) ( H 2 O ) ] - 2, 1990 J ack2 son [10 ],, Gd mol L - 1 ( Tf ), [ Gd ( Tf ) ( HCO 3 ) ] ; mol L - 1 [13 16 ] L n 2 Tf [ Gd (Cit ) (O H) ],,,,, Yb 2 Tf apo2tf, CL SM [11 ] apo2tf ( Tf R) K C lg K c = , lg K N = ( Eu 3 + ) lg K c = , lg K N = ( Tb 3 + ) [9 ], J ackson 1,,,,,, 2 Gd 3 + Tb 3 + Gd 3 + La 3 + Eu 3 + Tb 3 + Gd 3 + / g K ( ) (1) Tb2CIT ; (2) Tb c2tf ; (3) Tb2Asp ; (4) Tb2HAS

3 1 3, L n 2 Tf K562 Tf R, Fe 2 Tf Tf R K562, Tf R U - 87M G Yb 2 Tf, Yb 2 Tf,, Tf R, Yb 2 Tf, C La 3 +,, mol L - 1 ( ), DPPC DNA, [9 ] Ni 2 O 3 DNA, (200 [17, ] mol L - 1, Ca % ED TA, [9 ] [18 ],, La (NO 3 ) 3 ( < mol L - 1 ) [27 29 ],, 3198, 10-7 mol L - 1,,, ED TA [28 ] F T2IR Ce 3 +, (A FM) : GdCl 3, Gd 3 + DNA, Ce mol L - 1 DNA B,, [29, ] Ce 4 + mol L - 1, DNA, mol L - 1 DNA DNA, ED TA,, DNA,, 20 mg CeCl 3/ kg 70 d, [19 20 ], ( mol L - 1 La 3 + ) B EL K562, ( Hb) [21 22 ],, P 0. 2 < ( mol L - 1 ) Pa, Hb < 5 %,, Ce 3 +, Pr 3 +, Tb 3 + ( mol mol L - 1, L - 1 ) 20 % 30 %, ( < mol L - 1 ), Hb, Hb 4 DNA DNA,, (La 3 +, Gd 3 +, Yb 3 + ) 7701 H 2O 2, 2 [22 27 ] 100 g ml - 1 ) DNA, 5, CeCl 3 Hb, 20 mg kg - 1, Hb, 40 d ( <

4 Pa), Hb, 80 d, Hb Hb Hormesis,,,,, Hormesis [ 9 ],,,, Fe 2 + Fe 3 +, [31 33 ] 7 6 [34 38 ],, (biomarker),, 80 6, 1, 2 mg kg - 1, , La 3 + mg kg - 1[39 ], ng g - 1, Marker, ng g - 1, 012 mg kg - 1, La 3 +, 24171, ng g - 1,, 2 mg La (NO 3 ) 3 / kg La, 6 La > Ce > Pr, ( 2), 6,,,, 2 La, Ca, P ( 6 ) La/ (ng g - 1 ) Ca/ (mmol L - 1 ) P/ (mmol L - 1 ) Ca/ P 5. 71( 1. 96) ( 3. 96) ( 4. 37) 1. 76( 0. 49) ( 3. 81) ( 4. 11) ( 0. 78) 1. 24( 0. 14) La 3 + : La 3 + ( mol L - 1 ), mol L - 1 ; La 3 + 1, 252 D 3 La Hormesis, U M R 106, mol L - 1, mol L - 1, La 3 + G 0 / G 1 S, ; ( mol L - 1 ) [40 ] 2 2 mg kg - 1,, [1 ] Evans C H. Biochemistry of t he Lant hanides [ M ]. New York and London : Plenum Press, [ 2 ],,. LaCl 3, 1999, 17 ( ) : 37. Ca, K, Na [J ]. [3 ],,,. CeCl 3 Ce [J ]., 1999, 9 (6) : 512. [ 4 ],,. [J ]., 1999, 17 (

5 1 5 ) : 30. [5 ],,,., [22 ]. [J ]. ( ). [J ].. ( ), 2002, 34 (3) : 256. [ 6 ] Cheng Yi, Huo Qihua, L u Jingfen, et al. The transport ki2 netics of lanthanide species in a single erythrocyte probed by confocal laser scanning microscopy [ J ]. J. Biol. Inorg. Chin., 1999, 4 : 447. [ 7 ],,,. [D ]. ( [J ]., 1995, 27 (4) : 317. [8 ],,,. Eu (Cit) [J ]., 2002, 20 ( 4) : 342. [ 9 ]. ( ) [ M ]. :, [10 ] J ackson G E, Wynchank S, et al. Gadolinium ( ) com2 plex equlibria : The implications for Gd ( ) MRI contrast a2 gents [J ]. Magnetic Resonance in Medicine, 1990, 16 : 57. [ 11 ]. [ R ]. [ 27 ],,., [ 12 ],,,. [28 ]. [J ]., 1999, 20 (1) : 5. [ 13 ],,,. [ 29 ],,,. [J ]., 2001, 22 (1) : 26. [14 ],,. ( ) 2002, 20 (3) : 283. [J ]., 2001, 46 (5) : 394. [15 ] Du Xiulian, Zhang Tianlan, Yuan Lan, et al. Complexa2 tion of ytterbium to human transferrin and its uptake by K562 Cells [J ]. Eur J. Biochem. 2002, 269 : [ 16 ],,,. trated cerium chloride induces the conformational changes of U287M G [J ]., rat hemoglobin, t he hydrolysis of 2, 32DP G and t he oxida2 2003, 19 (7) : 902. [17 ],,. [J ]. Chemico2Biological Interactions, 2000, 125 : 191. [J ]. ( ), 2002, 34 (2) : 163. [18 ] [ 19 ],,,. ions induce hydrolysis of hemoglobin2bound 2, 32diphospho2 Wistar [J ]., 1999, 44 (14) : [20 ] Cheng Yi, Liu Maozi, Li Rongchang, et al. Gadolinium induces domain and pore formation of human eryt hrocyte membrane : an atomic force microscopic study [J ]. Biochim. Biop hys. Acta, 1999, 1421 : 249. [21 ] Cheng Y, Lin H K, L u J F, et al. The events relating to lanthanide ions enhanced permeability of human erythrocyte membrane : binding, conformational change, phase transi2 tion, perforation and ion transprot [J ]. Chem2Biol. Inter2 act., 1999, 121 (3) : [23 ],,,. (La 3 +, Ce 3 +, Y 3 +, Tb 3 + ) [J ]., 2000, 32 (3) : 203. [24 ]. ), [25 ] Liu H, Yuan L, Yang X, et al. La, Gd and Yb induced changes in mitochondrial structure, membrane permeability, cytochrome c release and intracellular ROS level [J ]. Chem. 2 Biol. Inter., 2003, 146 (1) : 27. [26 ] Wang Kui, Cheng Yi, Xiao Do, et al. Cell responses to lant hanides and potential p harmacological actions of lan2 thanides [J ]. Et al Ions in Biological Systems, 2003, 40 : 707. [J ]., 1998, 10 (4) : DNA [J ]., [30 ] Du X, Zhang T, Li R, et al. Nature of cerium ( )2 and lant hanum ( )2induced aggregation of human eryt hrocyte membrane proteins [J ]. J. Inorg. Biochem. 2001, 84 : 67. [31 ] Cheng Yi, Li You, Li Rongchang, et al. Orally adminis2 tion of heme2fe ( ), leading to changes of oxygen affinity [ 32 ],,,. [J ]., 1996, 14 (4) : [ 33 ] Cheng Yi, Lin Huakuan, Xue Deping, et al. Lant hanide glycerate (2,32DP G), conformational changes of globin and bidrectional changes of 2,32DP G2hemoglobin s oxygen affini2 ty [J ]. Biochim Biop hys Acta, 2001, 1535 : 200. [34 ],,. La ( NO 3 ) 3 La [J ]. ( ), 2003, 35 (6) : 622. [ 35 ],,,. [J ]., 2003, 48 ( 16) : [ 36 ] Zhang J C, Huang J, Xu S J, et al. Effects of Cu 2 + and p H

6 6 22 on osteoclastic bone resorption in vitro [J ]. Progress Nat. Sci., 2003, 13 (4) : 265. [ 37 ] Zhang J C, Xu S T, Wang K, et al. Effecdts of rare eart h ions on rabbit mature osteoclastic bone resorption function in vitro [J ]. Chinese Sci. Bull., 2003, 13 : 266. [ R ]., 2003, 49. [ 39 ]. [J ]. ( ), [ 40 ],,,. [38 ].., 2000, 16 (2), 273. Research Progress of Bioinorganic Chemistry of Rare Earths Li Rongchang 1, Yang Xiaoda 1, Wang Kui 1, Ni Jiazuan 2 ( 1. Depart ment of Chemical Biology, Peking U niversity School of Pharm aceutical Science, Beiji ng , Chi na ; 2. A pplied Chemist ry, Chi nese Academy of Sciences, Changchun , Chi na) Abstract : The st udies o n t he bioinorganic chem2 ist ry of rare eart hs ( R E) in China have been fo2 cused on t he critical problems around t he applica2 tio n o n agricult ure and medical p ractice. Af ter long and extensive research works, conclusive and informative result s have been summarized in t he following aspects : t he transmembrane transport of R E, t he speciation in t he animal body, t he inter2 Key words : bioinorganic chemistry ; cell chemistry ; rare eart hs Changchun Instit ute of action wit h cell membrane, t he effect on t he struc2 t ure and o xygen2carrier f unctio n of hemoglobin, t he influence on t he bone cells and bone structure and also R E accumulation on rat liver. The results might be used in t he risk evaluation of R E applica2 tio n in agricult ure and medicine. They will p ro2 mote t he develop ment of cytochemistry of RR as well , 3,,,, 3,,, : 11 ; 21 ; 31 ; 41 ; 51 ; 61 ; 71 ; 81 ; 91 X ; 101 ; 111 ( ) ; 121 ; 131 ; 141 : : A4 1 : ( , 6 ) : : A4 4 5 : : ( ) : 16 (130033) : : E2mail : ac. cn