6 cm, 1. 2 IAA, NAA, 2. 1

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1 30 6 ( ) V o l. 30 N o Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec Ξ 1, 2, 2, 3, 1, 4, 1 (1 ; 2 ; 3, ; 4, ) [],,,,,, ; 1g2 M S, 1g3M S ; 3 ; 1g2M S+ 62BA 1. 0 m ggl + IAA 0. 5 m ggl ; 1g3M S+ KT 0. 3 m ggl + NAA m ggl ; 1g3M S+ 62BA 0. 5 m ggl + NAA 0. 2 m ggl ; 1g4M S + NAA m ggl + IBA 0. 1 m ggl [] ; ; [ ] Q [ ] A [ ] (2002) (H ipp op hae rham noid es L. ) (E laeagnaceae) (H ipp op hae L inn),,, [1 ],,,, 1 2,,,, [1 6 ],, , 6 cm,, 70% 30 s, 0. 1% H gc l m in; (3 4 ) 4 8 m in, 4 5, 1. 2 M S, 1g2 M S, 1g3 M S, 1g4 M S ( ), IAA, NAA, IBA, 2, 42D, 62BA KT, 1g2M S IBA NAA 30 ggl, ph 5. 8, 6. 0 ggl (22 2), 11 h, lx ,, 11, 4, 90%,, 2 3 3, 100%, 4 5 d, Ξ [ ] [ ] ( ) [ ] (1969- ),,,,

2 6 : 163,,, 15 d 2. 5 cm, 5 9 1,,,, 6 8,,, 3 g T im e of inoculated 1 T able 1 Effect of different inoculation tim e on the grow th of buds gd T im e of germ i2 nation g% Percent of germ i2 nation 10 d 15 d gcm gcm Grow th Grow th after after 5 10 days days Rem arks, H igh rates of po llution, grow ing rap idly Grow ing slow ly N eed to relieve the do rm ancy , Germ inating early and grow ing rap idly H igh rates of po llution , H igh rates of po llution, faciliating the vitrification , Serious braw n change and difficult gem ination Basic m edium, 2 ( 2) T able 2 T he effect of basic m edium on the grow th of buds 15 d gcm Grow th after 15 days A verage N o. of regeneration shoo ts per exp lant M S g2M S Rem arks Shoo ts change brow n and die at later stage T he status of grow th is the best 1g3M S T he status of grow th is better 1g4M S T he status of grow th is slow (62BA 0. 5 m ggl, IAA 0. 2 m gg L ) M S, 1g2M S, 1g3M S, 1g4M S 15 d,, 1g2M S,,,, M S,,,,, , 0. 3, 0. 5, 0. 7, 1. 0, 1. 5, 2. 4 m ggl 62BA 0. 2 m ggl IAA,, 3,,,,, 3 cm, ;, 62BA 0. 2 m ggl,, IAA, 0. 05, 0. 1, 0. 2, 0. 5 m ggl IAA m ggl 62 BA, m ggl,,, [7 ],, 1g2M S, 62BA, IAA, 20 d,, 3

3 164 ( ) 30 g(m g L - 1 ) Ho rmones 62BA KT NAA IAA 3 T able 3 Effect of ho rmones on p ro liferation of tube2p lantlets treatm ent 20 d gcm A verage heigh t of shoo ts afer 20 days A verage N o. of spouted shoo ts per tube2p lantlet ,, 20 d 1g2M S + 62BA 4 m ggl + IAA 0. 2 m ggl,,,,, ; 1g2M S + KT 1 m ggl + NAA 0. 2 m ggl,, ; 1g2M S+ 62BA m ggl + IAA 0. 5 m ggl,,,, 0. 5 m ggl IAA m ggl 62BA M edium,,, 62BA 1. 0 m ggl,,, 1g2M S+ 62BA 1. 0 m ggl + IAA 0. 5 m ggl , 1g2 M S, 1g3 M S,, 4 4 T able 4 T he effect of different ho rmones on inducem ent of calluses g(m g L - 1 ) Ho rmones IAA NAA 62BA KT g% R atio of bud inducem ent g% Ratio of death 1g2M S Developm ent and grow th of callus Changing brow n and dying at later stage 1g2M S Grow ing slow ly 1g3M S Inducing rap idly 1g3M S Inducing rap idly 1g3M S , IAA 0. 2 m ggl, 62BA 1 m ggl 1g3M S + 62BA 0. 5 m ggl + NAA 0. 1 m ggl,, 20 d 62BA 0. 5 m ggl, M edium Changing brow n and dying at later stage 1g3M S+ KT m ggl + NAA m gg L, 80%,, 2. 3, 5 5 T able 5 Subculture of buds and inducem ent of calluses inoculated buds 1g2M S+ 62BA 1 m ggl + IAA 2 m ggl g3M S+ 62BA 0. 5 m ggl + NAA 0. 2 m ggl 10 8 callus induced D evelopm ent and grow th of callus, Grow ing rap idly at initial stage, dying gradually at later stage, Grow ing rap idly at initial stage, grow ing slow ly at later stage 1g3M S+ KT 0. 2 m ggl + NAA m ggl 12 10, rap idly, Grow ing inducing green do ts of buds : N o te: Exp lants are stem s and leaves. 5,,

4 6 : 165, 15 d,,, 40 d 2. 0 cm,,,,, 20 d 1. 2 cm, 2 3, 0. 8 cm 1. 5 cm, 5 mm,, d 1 2 cm,,,, M S 2,,,, ; 1g3M S,, g4M S NAA IBA,, NAA m ggl, IBA 0. 1 m ggl,,, :,, ;,,, 3,,, :, 1g4 1g3M S ;,, ; 10 d ;,,, [4, 8 ] ;,, 6 9, (5 ) 5 d,, 3. 2 (6 8 ), [9 ] ;,, ;,,,, 3. 3,,,,,,, 3. 1 [ ] [ 1 ],,,. [J ]., 1998, 11 (3) : [ 2 ],,. [J ]., 1999, 12 (1) : [ 3 ],. [J ]., 2001, 21 (2) : [ 4 ],. [J ]., 2001, 21 (2) : [ 5 ],. [J ]., 1989, 25 (1) : 42. [ 6 ],. [J ]., 2000, 13 (1) : [ 7 ]. [M ]. :, [ 8 ]. [J ]., 1999, 35 (6) : [ 9 ],,,. [J ]., 1998, 5:

5 166 ( ) 30 Studies on tissue cu ltu re of fine variety of H ipp op hae rham noid es L. from R u ssia KANG B ing 1, ZHANG Guang- jun 2, L U Y ue- ling 2, L IW en-hua 3, ZHANG X iao-hong 1, ZHANG J in-xue 4, CAO J ian- jun 1 (1 College of L if e S ciences; 2 College of R esource and Env ironm ent; 3 College of Forestry, N orthw est S ci2t ech U niversity of A g riculture and Forestry, Yang ling, S haanx i , China; 4 Forestry B ureau of T ianzhu County, T ianzhu, Gansu , China) Abstract: C lu ster adven titiou s buds and callu ses w ere induced from do rm an t and grow ing stem segm en ts of fine variety nam ed YOU SH EN G of H ipp op hae rham noid es L. from R u ssia. T he tissue cu ltu re abou t o ther fine varieties can also be studied. budsw h ich w ere induced from do rm an t stem segm en ts w ere mo re, ob tained easily. T he resu lts show ed that the amoun t of adven titiou s the clone regenerated p lan ts also w ere T he basic cu ltu re m edium is 1g2 M S m edium, the basic m u ltip lication m edium is 1g3 M S m edium. M arch is the best inocu lation tim e. T he inocu lation m edium is 1g2 M S+ 62BA 1. 0 m ggl + IAA 0. 5 m ggl, the callu s inducing m edium is 1g3M S+ KT 0. 3 m ggl + NAA m ggl, the m u ltip lication m edium is 1g3 M S + 62BA 0. 5 m ggl + NAA 0. 2 m ggl, the m edium fo r roo ting is 1g4 M S + NAA m ggl + IBA 0. 1 m ggl. Key words: H ipp op hae rham noied s L. ; tissue cu ltu re; callu s,,,,,,,,,, ( ) ( )