( P < 0105) ; WANG Cong LIU Qiang DONG Kuan2hu ZHAO Xiang HUANG Ying2xiang DONG Qun

Μέγεθος: px

Εμφάνιση ξεκινά από τη σελίδα:

Download "( P < 0105) ; WANG Cong LIU Qiang DONG Kuan2hu ZHAO Xiang HUANG Ying2xiang DONG Qun"

É Φωτόπουλος
7 χρόνια πριν
Προβολές:

1 2008,22 (6) : Journal of Nuclear Agricultural Sciences 887 : (2008) (, ) :16 4,,, mgΠkg, :8 16mgΠkg 24mgΠkg ( P < 0105) ; ( P < 0105) ; Π ( P < 0105),16 24mgΠkg 8mgΠkg ( P < 0105) ( P < 0105), 16 24mgΠkg 8mgΠkg ( P < 0105) ; ( GOT) ( GPT) (AKP) (ACP) (CDH) ( P > 0105) ; (CP) (SOD) ( GSH2px) ( P < 0105) ; ( P < 0105) ( P < 0105), ;, ( P > 0105), 8 16mgΠkg : ; ; ; ; EFFECTS OF RUMEN BY2PASS COPPER ON NUTRIENT METABOLITY OF DIET AND SERUM PHYSICO2CHEMICAL PARAMETERS IN SIMMENTAL STEER WANG Cong LIU Qiang DONG Kuan2hu ZHAO Xiang HUANG Ying2xiang DONG Qun ( College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi ) Abstract :Sixteen Simmental steers consuming a corn straw diet were randomly assigned in a block design to treatments to evaluate the effects of different rumen by2pass copper levels on nutrients digestibility, nitrogen balance and serum parameter. Treatments consisted of 0 (control), 8, 16 or 24mg of supplemental Cu (as rumen by2pass copper) per kg diet dry matter. The results showed that the digestibility of organic matter in group supplemented with 8 and 16 mg CuΠkg diet dry matter were higher than control and 24 mgπkg groups( P < 0105). The digestibility of crude protein, ether extract, nitrogen free extract, neutral detergent fiber and acid detergent fiber in treatments increased significantly ( P < 0105) compared with those of control. Digestible nitrogen, retention nitrogen and the utilization of nitrogen in group supplemented 16 and 24 mg CuΠkg diet dry matter were higher than those of 8 mg group ( P < 0105). The apparent deposit rate of minerals and the concentrations of serum triglycerides, glucose, cholesterol total, albumin and total protein in treatments increased significantly ( P < 0105) compared with those of control. Urine nitrogen concentrations in treatments decreased, 16 and 24 mg groups obviously lower than control and 8 mg group ( P < 0105). The activities of serum glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, acid phosphatase and lactate dehydrogenase were not affected by supplemental Cu ( P > : : : ( ) ; (412551) : (19732),,,,, E2mail ' China Academic Journal Electronic Publishing House. All rights reserved.

2 ), however, the activities of serum ceruloplasmin, superoxide dismutase and glutathione peroxidase increased by supplemental Cu ( P < 0105) significantly. The concentrations of serum maleic dialdehyde significantly decreased by supplemental Cu (P < 0105), and serum Cu, Fe and Zn concentrations in treatments were significantly higher than control (P < 0105), however, no significant difference between treatments. Serum Mn concentrations decreased with no significant difference ( P > 0105). These data indicate that nutrients digestion and utilization are improved by supplemented 8 16mg Cu per kg diet dry matter. The activities of serum enzyme increased and maleic dialdehyde concentration decreased by supplemented 16 mg CuΠkg diet dry matter. The results indicated that optimum rumen by2pass Cu supplemented dose was 8 16 mg Cu per kg diet dry matter. Key words :Simmental steer ; rumen by2pass copper ; digestibility ; nitrogen balance ; physico2chemical parameters,, [1 ],,,,,,, [2 ],,,, [3 ], [4 10 ],,,,, kg 215, 4,, mgΠkg (rumen by2pass copper), Cu 10 % 10d, 10d ( ) 1 3 ( ),,, 3kg ( ) 6kg ( ), Table 1 Ingredient and nutrient composition of basal diet (dry matter basis) ingredient of basal diet ( %) nutrient composition corn straw 6010 net energy of maintain and finishing 2 (MJΠkg) 6154 corn grain 2018 crude protein, ( %) wheat bran 410 neutral detergent fiber, ( %) soybean meal 616 acid detergent fiber, ( %) cottonseed cake 418 Ca ( %) 0175 rapeseed meal 210 P( %) 0152 calcium carbonate 015 S( %) 0141 salt 014 Se (mgπkg) 0127 sicalcium phosphate 0135 Cu(mgΠkg) 5187 mineral and vitamin mix Zn(mgΠkg) Fe (mgπkg) Mn (mgπkg) Mo (mgπkg) 1179 :1 : kg : A 150, 000 ; D 60, 000 ; E 750 ; 1500mg ; 1500mg ; 2000mg ; 1215mg ; 15mg ; 5mg ;2 :, Note :1 :One kilogram mineral and vitamin mix contained as : V A 150, 000 IU ; V D3 60, 000IU ; V E 750 IU ; Fe 1500mg ; Zn 1500mg ; Mn 2000mg ; I 1215mg ; Se 15mg ; Co 5mg ;2 :The value of net energy of maintain and finishing was caculated and the other data in this colomu were measured.

3 %, 2, 1, ,, 10d ( dry matter, DM) (organic matter, OM) (crude protein, CP) (ether extract, EE) (nitrogen free extract, NFE) (Ca) ( P) [11 ] ; ( neutral detergent fiber, NDF) ( acid detergent fiber, ADF) Van Soest [12 ] ; (Cu) anova LSD (Fe) ( Zn) (Mn) AA22610, (S), (Mo) [11 ] 1d 40 ml, 3000 rπmin 15 min,, Eppendorf - 40 ( Glucose, GLU) ( Total protein, TP) (Albumin, ALB) ( Serum urine nitrogen, SUN) ( Total triglyceride, TG) ( Total cholesterol, TC) (maleic dialdehyde, MDA) (superoxide dismutase, SOD) ( glutathione peroxidase, GSH2px ) (ceruloplasmin, Cp) (alkaline phosphatase, AKP) (acid phosphatase, ACP) (lactate dehydrogenase, LDH) (glutamate pyruvate transaminase, GPT) ( glutamate oxaloacetate transaminase, GOT), UV ; WFY228 [13 ] 114 SPSS1010 One2way ,8 16mgΠkg 24mgΠkg ( P < 0105) ; ( P < 0105), ( P > 0105) 2 Table 2 Effects of supplemental rumen by2pass copper on nutrient apparent digestibility in Simmental steer ( %) nutrient organic matter crude protein ether extract nitrogen free extract neutral detergent fiber acid detergent fiber b a a b b a a a b a a a b a a a b a a a b a a a : 0105 Note :The data followed by common small letters indicated no significant at 0105 levels. The same as other tables , ( P > 0105), ( P < 0105),16 24mgΠkg ( P < 0105) ; (DN) (RN) RNΠDN ( P < 0105),16 24mgΠkg 8mgΠkg ( P < 0105) 213 4, Cu P Fe Zn Mn Mo ( P < 0105), ( P > 0105) ; Ca S, Ca 16 24mgΠkg 8mgΠkg ( P < 0105) ; S 16mgΠkg 8 24mgΠkg ( P < 0105) 214 5, TG GLU TC ALB TP ( P < 0105), ; SUN,16 24mgΠkg 8mgΠkg ( P < 0105),24mgΠkg 16mgΠkg ( P > 0105) GOT GPT AKP ACP LDH ( P > 0105) Cp SOD

4 Table 3 Effects of supplemental rumen by2pass copper on the nitrogen balance in Simmental steer nitrogen balance intake N(gΠd) feces N(gΠd) urine N(gΠd) digestible N(gΠd) retenion N(gΠd) Π retention NΠdigestible N ( %) a a a a a b b c a ab b b c b a a c b a a c b a a 4 Table 4 Effects of supplemental rumen by2pass copper on the apparent deposit rate of mineral in Simmental steer ( %) mineral Cu Ca P Fe Zn Mn Mo S b a a a c b a a b a a a b a a a b a a a b a a a b a a a c b a b 5 Table 5 Effects of rumen by2pass copper supplemental on serum parameters in Simmental steer serum parameter TG (mmolπl) GLU (mmolπl) TC (mmolπl) ALB (gπl) TP (gπl) SUN (mmolπl) GPT ( IUΠL) GOT ( IUΠL) AKP ( IUΠL) ACP ( IUΠL) LDH ( IUΠL) Cp ( IUΠL) SOD (UΠml) GSH2px (UΠml) MDA (mmolπl) Cu ( gπdl) Fe ( gπdl) Zn ( gπdl) Mn ( gπdl) 0 8mgΠkg 16mgΠkg 24mgΠkg b a a a b a a a b a a a b a a a b a a a a a b c a a a a a a a a a a a a a a a a a a a a b a a a b a a a c b a b a a b b b b a a a b a a a b a a a a a a a, ( P < 0105) ; GSH2px,,24mgΠkg 8 16mgΠkg ( P < 0105) ;MDA, ( P < 0105), ( P > 0105) Cu Fe Zn, ( P < 0105), ( P > 0105) ; Mn, ( P > 0105)

5 Journal of Nuclear Agricultural Sciences 2008,22 (6) : ,,,,16 24mgΠkg ; Π,16 24mgΠkg 8mgΠkg, Radecki [14 ],, 16mgΠkg,24mgΠkg,, 16mgΠkg,, 312,,,,,,, [15 ] 8 16mgΠkg 8, 16mgΠkg,, mgΠkg :, [16 ],,,,,,, C, [17 ],,,,,Lauridsen [18 ], [19 ],, H 2 O 2,,,, [20 ],,,,,,,,,,,,,, ;,, [21 ] 4, [ 1 ],,.., 2006, (2) : [ 2 ].. :, 1996, [ 3 ],,,,,.., 2006, 27 (13) : [ 4 ],,,.., 2004, 25 (5) : [ 5 ],,,,,.., 2005, 24 (5) : [ 6 ],,.., 2002, 23 (8) : ( 828 )

6 828,,,, : [ 1 ] Wesley I J, Larroque O, Osborne B G, Azudin N, Allen H, Skerritt J H. Measurement of gliadin and glutenin content of flour by NIR spectroscopy. Journal of Cereal Science, 2001, 34 (2) : [ 2 ] Brian G, Osborne, Thomas Fearn, Andrew R Miller, Stuart Douglas. Application of near infrared reflectance spectroscopy to the compositional analysis of biscuits and biscuit doughs. Journal of the Science of Food and Agriculture, 35 (1) : [ 3 ] Wehling R L, Jackson D S, Hamaker B R. Prediction of corn dry2 milling quality by near2infrared spectroscopy. Cereal Chemistry, 1996, 73 (5) : [ 4 ],,. BP Π.,2007,26 (6) : [ 5 ],,.., 2005,19 (3) : [ 6 ],Nagamine T,,,,Hisashi Yoshida..,2005,31 (3) : [ 7 ],,,,,,..,2007,22 (3) : [ 8 ],,,,,,..,2007,21 (5) : [ 9 ]. AOAC. :,1991 [10 ],,,,Anna McClung.., 1999,11 (3) : [11 ],,,,..,2004, 24 (1) :45 49 [12 ],,,..,2004,24 (2) : [13 ],,..,2007,22 (3) : [14 ],,,..,2006,20 (4) : [15 ],,,,..,2006,39 (4) : [16 ],,..,2004,24 (10) : [17 ],,..,2001,21 (4) : [18 ],,,..,1990, (16) :37 48 [19 ]..,1996, (1) :24 25 [20 ],,..,2001,32 (3) :68 70 Journal of Nuclear Agricultural Sciences 2008,22 (6) : ( 891 ) [ 7 ] Du Z, Hempken R, Jackson J, Trammell D. Utilization of copper in copper proteinate, copper lysine and cupric sulfate using the rat as an experimental model. Journal of Animal Science, 1996, 74 : [ 8 ],,,,.., 2006, 42 (23) : [ 9 ],,,,,.., 2007, 49 (6) : [10 ],,,,.., 2008, 22 (2) : [11 ].. :,1996 : [12 ] Van Soest P J, Robertson J B, Lewis B A. Methods for dietary fiber, neutral detergent fiber and non2starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 1991, 74 (6) : [13 ],,,,.., 2008, 22 (2) : [14 ] Radecki. Effect of dietary copper on intestinal mucous enzyme activity, morphology and turnover rates in waling pigs. Journal of Animal Science, 1992, 70 : [15 ] Engle T E, Spears J W, Armstrong T A, Wright C L, Odle J. Effects of dietary copper source and concentration on carcass characteristics and lipid and cholesterol metabolism in growing and finishing steers. Journal of Animal Science, 2000, 78 : [16 ] Tetlow L C, Woolley D E. Histamine stimulates the proliferation of human articular chondrocytes in vitro and is expressed by chondrocytes in osteoarthritic cartilage. Annals of the Rheumatic Diseases, 2003, 62 (10) : [17 ] Blakley B. Immnotoxicity of pesticides : a review. Toxicol and Health, 1999, 15 : [18 ] Lauridsen C, Hojsgaard S, Sorensen M T. Effects of diretary rapeseed oil, vitamin E, and copper on the perforance and antioxidative and oxidative status of pigs. Journal of Animal Science, 1999, 77 : [19 ],,,. SOD., 2005, 41 (3) : [20 ] Paynter D I. The role of dietary coper, manganese, selenium and vitamin E in lipid peroxidationin tissues oh the rat. Biological Trace Element Research, 1980, 2 : [21 ] Elvehjem C A, Hart E B. The necessity of copper as a supplement to iron for hemoglobin formation in the pig. Journal of Biological Chemistry, 1932, 95 :

Σχετικά έγγραφα

LIU Qiang HUANG Ying2xiang WANG Cong ZHOU Yi WANG Hao

LIU Qiang HUANG Ying2xiang WANG Cong ZHOU Yi WANG Hao 2008,22 (2) :233 237 Journal of Nuclear Agricultural Sciences 233 :100028551 (2008) 022233205 (, 030801) : 16 420kg 215, 4,, 0 013 016 019mgΠkg,,013 016mgΠkg ( P < 0105) ;016 019mgΠkg ( P < 0105) ;016mgΠkg