EFFECTS OF SELENIUM PLEX ON SERUM REPRODUCTIVE HORMONE LEVEL DURING ESTROUS CYCLE IN SIMMENTAL HEIFERS

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1 2007,21 (3) : Journal of Nuclear Agricultural Sciences 317 : (2007) (, ) :, ( + ) ( mgΠkg), LH FSH P 4 E 2, mgΠkg 019mgΠkg,019mgΠkg ( P < 0105) 016mgΠkg,, 0167mgΠkg : ; ; ; EFFECTS OF SELENIUM PLEX ON SERUM REPRODUCTIVE HORMONE LEVEL DURING ESTROUS CYCLE IN SIMMENTAL HEIFERS LIU Qiang HUANG Ying2xiang YUE Wen2bin WANG Cong WANG Hao ( College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi ) Abstract :Sixteen Simmental heifers were chosen and allocated into four treatments and supplemented selenium with levels of 0, 013, 016 and 019mgΠkg by selenium plex to evaluate the effects of selenium plex on reproductive hormone excretion during the oestrous cycle. The results showed that serum estradiol, luteinizing2hormone, follicle2stimulating hormone and progesterone concentrations were increased significantly ( P < 0105) by supplemental selenium plex at 013 and 016mgΠkg DM treatments compared with 019mgΠkg and control group ; and 019mgΠkg group was significantly higher than control group ( P < 0105). It was indicated that selenium plex had positive effects on reproductive hormone during estrus cycle by supplemental selenium at 016mgΠkg. Considering the selenium content in basal diet, the optimum selenium level of diet is 0167mgΠkg DM. Key words :Selenium plex ; estrous cycle ; reproductive hormone ; simmental heifer GSH2px,, [1,2 ], [3 5 ] Henry,, GSH2px 100 %, 290 % [6 ],, (LH FSH P 4 E 2 ),, 1 111, kg 4, ( + ) ; : : (02EFN ) ; (042S2640) : (19712),,,,,,E2mail :

of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801) Abstract :Sixteen Simmental heifers were chosen and allocated into four treatments and supplemented

2 318 21, mgΠkg, 1gΠkg, Alltech,, 30d, 21d 112 ( %) : (V A IUΠkg V D IUΠkg V E 750IUΠ kg Fe 1500mgΠkg Cu 400mgΠkg Zn 1500mgΠkg Mn 2000mgΠkg I 1215mgΠkg Co 5mgΠkg) 018 : 6154MJΠkg % % % 0175 % 0152 % 0107mgΠkg 3 (07 :00 15 :00 23 : 00),,,, 113,,, 21h, 3h 1, 1, 1d 09 :00,, 20 ml, 30min, 2500rΠmin 10min,, GC (luteinizing2hormone, LH) (follicle2 stimulating hormone,fsh) (estradiol, E 2 ) (progesterone,p 4 ), 115 SPSS 1010 One2 way2anova LSD, SPSS 1010 Regression Curve Estimation , mgΠkg LH 019mgΠkg,019mgΠkg ( P < 0105) 12h LH, mgΠkg ( P < 0105) 10d 2, 16d 3, mgΠkg 019mgΠkg,019mgΠkg ( P < 0105) 17d, LH : y = x x ( R 2 = ) 1 Table 1 Effects of different supplemental selenium plex levels on serum LH at estrous period of Simmental heifers (miuπml) item b a a a c a a a c a a ab c a a b b a a ab c a a b c a a b c a a b c a a b c a a b 21 ( average) c a a b : ( P < 0105), Note : Data in the same row with different superscripts letters are significantly different ( P < 0105),the same as in the following tables.

(luteinizing2hormone, LH) (follicle2 stimulating hormone,fsh) (estradiol, E 2 ) (progesterone,p 4 ), 115 SPSS 1010 One2 way2anova LSD, SPSS 1010 Regression Curve Estimation 2 211 1 1, 013 016mgΠkg LH

3 mgΠkg ( P < 0105) 16 FSH : y = x x ( R 2 = ) Fig. 1 Effects of selenium plex on LH excretionduring the oestrous cycle of Simmental heifer 2 2, mgΠkg FSH 019mgΠkg,019mgΠkg ( P < 0105) 1 7, 8, mgΠkg 019mgΠkg ( P < 0105) 9 10, 12 15, mgΠkg 2 Fig. 2 Effects of selenium plex on FSH excretion 2 Table 2 Effects of supplemental selenium plex levels on serum FSH at estrous period of Simmental heifers (miuπml) c a a b c a a b c a a b c a a b c a a b c a a b c a a b c a a b b a a b b a a b b a a b 21d( average) c a a b , mgΠkg P 4 ( P < 0105) 1 7d,8 15d, mgΠ kg ( P < 0105), P 4 : y = x x ( R 2 = 01974) , mgΠkg E 2 019mgΠkg,019mgΠkg ( P < 0105) 12h, mgΠkg 019mgΠkg,019mgΠkg ( P < 0105), 4,5 8d, mgΠkg 019mgΠkg,019mgΠkg ( P < 0105) E 2 : y = x x ( R 2 = )

4 Fig. 3 Effects of selenium plex on P 4 excretion 4 Fig. 4 Effects of selenium plex on E 2 excretion 3 Table 3 Effects of supplemental selenium plex levels on serum P 4 at estrous period of Simmental heifers(ngπml) b a a ab b a a ab b a a ab b a a ab b a a ab b a a ab b a a ab b a a ab b a a b b a a b b a a b b a a b b a a b b a a b b a a b b a a c 21d( average) b a a ab FSH 3 [10 ] Akbar Bolt [11,12 ],, FSH LH FSH,, [7 ] Madej 5, ngΠml, 12h [7 ] P 4, P 4 FSH LH P 4 1 7,8 15,, ngΠml [8 ] 16, Larsson LH, LH [13 ] Smith Beckers 714h [9 ],, P 4, 6 12h P 4 [14,15 ]

0138 0108 ab 3 0138 0115 b 0155 0114 a 0163 0113 a 0148 0112 ab 6 0137 0117 b 0156 0121 a 0168 0111 a 0152 0113 ab 9 0142 0112 b 0154 0113 a 0163 0114 a 0145 0111 ab 12 0128 0111 b 0148 0112 a 0157

5 Journal of Nuclear Agricultural Sciences 2007,21 (3) : Table 4 4 Effects of supplemental selenium plex levels on serum E 2 at estrous period of Simmental heifers(pgπml) c a a b c a a b c a a b c a a b c a a b c a a b c a a b c a a b c a a b c a a b c a a b c a a b 21d(,average) c a a b E 2 P 4,P 4 E 2,, [16 ], [17 ] P 4,12h, [18 ], P 4 E 2 Smith E 2, 1 1d, 2 6 8d,, 016mgΠkg DM, 0167mgΠ kgdm : [ 1 ],,,. E., 2000, (5) : [ 2 ] Erskine R J, Eberhart R J, et al. Induction of Escherichia coli mastitis in cows fed selenium2deficient or selenium2supplemented diets. AmJ Vet Res, 1989, 50 : [ 3 ] Church D C, Hansard S L, et al. Whanger. The trace elements. In : Church D C ed. Digestion Physiology and Nutrition of Ruminants. O &B Books. Inc Corvallis OR, 1971, 100 l56 [ 4 ] Paulson G D, Bauman C A, Pope A L. Metabolism of 75 Se2selenite, 75 Se2selenate, and 75 Se2selenomethionine and 35 S2sulfate by rumen microorganisms in vitro. J Anim Sci, 1968, 27 : [ 5 ] Hudman J F, Glenn A R. Selenite uptake and incorporation by Selenomonas ruminatium. Arch Microbiol, 1984, 140 : [ 6 ] Henry P R, Ammerman C B. Selemium bioavailability in bioavailability of nutrents for animals, Amino Acids, Minerals and Vitamins C. New York : American Acidemin Press, 1995 [ 7 ],,,.., 1996, 11 (3) : [ 8 ] Madej A, Stupuicki R. Peripheral plasma levels of LH in the cow throught the estrus cycle. Endorkrionlogie, 1975, 65 (2) : [ 9 ] Larsson B. Determination of ovulation by ultrasound examination and its relation to the LH2peak in heifers. Zentralbl Veterinarmed A, 1987, 34 (10) : [10 ].. :, 1993 [11 ] Akbar A M, Reichert L E Jr, et al. Serum levels of follicle2stimulating hormone during the bovine estrus cycle. J Anim Sci, 1974, 39 (2) : [12 ] Bolt D J, Rollins R. Development and application of radioimmunoassay for bovine follicle2stimulating hormone. J Anim Sci, 1983, 56 (1) : [13 ],.. ( ), 2005, 23 (2) : ,142 [14 ] Smith J F, Fariclough R J, Payne E. Plasma hormone levels in the cow. 11 changes in progestone and oestrogen during normal estrus cycles. New Zealand J Agri Res, 1975, 18 : [15 ] Beckers J F, Ballman P, Ectors F. Radioimmunoa2ssay of progesterone in the cow. Sci Naturales, 1975, 28 (3) : [16 ] Henricks D M, Dickey J F, et al. Plasma estrogen and progesterone levels after mating and during late pregnancy and postpartum in cows. Endocrinology, 1972, 90 (5) : [17 ] Hafez E S E. Reproduction in Farm Animals 4th ed Edition. Publisher : Lea & Febigeer, 1980, 103 [18 ] Melampy R M, Emmerson M A, et al. The effect of progesterone on the estrous response of estrogencondition ovarieotomized cows. J Anim Sci, 1957, 16 :

12 17164 1164 c 32117 1137 a 36109 1142 a 31119 1149 b 15 4130 1128 c 17158 1154 a 20179 1138 a 16148 1137 b 18 5106 1135 c 17173 1144 a 19161 1129 a 15139 1141 b 21 3147 1146 c 17145 1133 a 19152

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