LIU Qiang HUANG Ying2xiang WANG Cong ZHOU Yi WANG Hao

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1 2008,22 (2) : Journal of Nuclear Agricultural Sciences 233 : (2008) (, ) : kg 215, 4,, mgΠkg,, mgΠkg ( P < 0105) ; mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105), ;016mgΠkg GPT GOT GSH2px GSH ( P < 0105) mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105) 016mgΠkg,, 0160mgΠkg : ; ; ; EFFECTS OF SELENIUM METHIONINE ON NUTRIENT METABOLITY AND SERUM PHYSICO2CHEMICAL PARAMETERS IN SIMMENTAL STEER LIU Qiang HUANG Ying2xiang WANG Cong ZHOU Yi WANG Hao ( College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi ) Abstract :Sixteen Simmental steers ( 420kg and 215 years old) were stratified by weight and randomly assigned in a block design to treatments. Treatments consisted of 0 (control), 013, 016 or 019mgΠkg of supplemental Se ( selenium methionine) diet DM to study the effects of selenium methionine on nutrient metabolic rate and serum biochemical parameters. The digestibility of organic matter, crude protein, ether extract, neutral detergent fiber and acid detergent fiber in groups supplemented Se 013 and 016mgΠkg were higher than control group ( P < 0105). Digestion nitrogen, retention nitrogen and the utilization of nitrogen in groups supplemented of Se, 016 and 019mgΠkg were higher than control group ( P < 0105). In group of supplemented 016mgΠkg Se, the apparent deposit rate of Ca, P, Se, Cu, Mo and Mn increased ( P < 0105), however S and Zn apparent deposit rate was not affected ( P > 0105). Concentrations of serum triglycerides, glucose, albumin, total protein were higher than those of control groups ( P < 0105). The activities of serum GPT, GOT, GSH2px and the concentration of GSH in group supplemented 016mgΠkg Se were higher than those of control group ( P < 0105). The concentrations of serum MDA in group supplemented Se of 016 and 019mgΠkg decreased ( P < 0105). The concentration of GSH in group supplemented 016mg Se were higher than control group ( P < 0105). Nutrients digestion and utilization improved by supplemented Se of mg. The activities of serum enzyme increased and MDA concentration decreased with supplemented 016mgΠkg. The optimum supplemented Se dose is 016mg SeΠkg DM. Key words :Simmental steer ; selenium methionine ; metabolic rate ; serum biochemical parameters : : (02EFN ) (411480) : (19712),,,,, Tel : ; E2mail :

2 [1 ] [2 ] [3 ],, 100 %, 105 %, 245 %, 290 % [4 ] Fisher,,,, [5 ] (S), (Mo) [13 ] [6 ] [7,8 ], [9 ], kg %, 40 % 2018 % 410 % 616 % 418 % 210 % 016 % 014 % (VA IUΠkg VD IUΠkg VE 750 IUΠkg Fe 1 500mgΠkg Cu 400mgΠkg Zn 1 500mgΠkg Mn 2 000mgΠkg I 1215mgΠkg Co 5mgΠkg) 018 % 6154 MJΠkg % % % 0175 % 0152 % 0107mgΠkg 5800mgΠkg, 1 500mgΠkg 112 4, ; mgΠkg 3 (07 :00 15 :00 23 :00),,, 3kg, 6kg,,,, 10d, 10d, 2 %, 2, 1, ,, 10d 113 (DM) (OM) (CP) (Ca) ( P) [10,11 ] ; NDF ADF Van Soest [12 ] ; ( Cu) ( Fe) (Zn) (Mn) AA22610, ( %) = ( - - )Π 100 % 1d 40ml, 3000rΠmin 15min,, Eppendorf - 40 ( GLU) ( TP) (ALB) ( SUN) ( TG) (TC) (MDA) ( GSH2px) (ALTΠGPT) (ASTΠGOT) ( GSH), UV ; WFY2 28 [13 ] 114 SPSS1010 One2way2 anova, Duncan, P < 0101 ( ), P < 0105 ( ) , mgΠkg, mgΠkg 019mgΠkg ( P < 0105) ; ( P < 0105), 212 2, ; Π, mgΠkg ( P < 0105)

3 Table 1 Effects of selenium methionine on nutrient apparent digestibility in Simmental steer ( %) organic matter ( %) crude protein ( %) ether extract fat ( %) nitrogen free extract ( %) neutral detergent fiber ( %) acid detergent fiber ( %) : ( P < 0105) b a a ab b a a ab b a a b b a a a c a a b c a a b Note : different superscripts letters in the same row means are significantly different ( P < 0105). The same as following tables. nitrogen intake (gπd) feces nitrogen(gπd) urine nitrogen (gπd) 2 Table 2 Effects of selenium methionine on nitrogen balance in Simmental steer digestible nitrogen (gπd) retenion nitrogen (gπd) Π retenion nitrogenπdigestible nitrogen ( %) a a a a a a b ab b b ab a c b a a c a a a c ab a a 213 3, mgΠkg 013mgΠkg,013mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105) ; mgΠkg 019mgΠkg ( P < 0105) ; ;016mgΠkg 013mgΠkg ( P < 0105) ; 016mgΠkg 019mgΠkg ( P < 0105) ;, ( P > 0105) 3 Table 3 Effects of selenium methionine on the apparent deposit rate of mineral in Simmental steer ( %) Se Ca P Cu Fe Zn Mn Mo S c b a a c b a a c b a b b a a b b b a ab a a a a c b a a b ab a b a a a a 214 4, ( P < 0105),016mgΠkg 013mgΠkg ( P < 0105) ;016mgΠkg ( P < 0105) ; mgΠkg ( P < 0105) ; ( P > 0105) ; mgΠkg GPT ( P < 0105) ; GOT ( P < 0105), ( P > 0105) ; mgΠkg GSH2px 019mgΠkg ( P < 0105) ;MDA

4 236 22, mgΠkg ( P < 0105) ; 016mgΠkg GSH ( P < 0105), mgΠkg ( P < 0105) 4 Table 4 Effects of selenium methionine on serum physico2chemical parameters in Simmental steer gotal triglyceride (mmolπl) glucose (mmolπl) glbumin(gπl) gotal protein(gπl) gerum urine nitrogen(mmolπl) glutamate pyruvate transaminase ( IUΠL) glutamate oxaloacetate transaminase ( IUΠL) glutathione peroxidase (UΠml) Maleic dialdehyde (nmolπl) glutathione (mgπl) Se ( gπdl) c b a ab b ab a ab b ab a a b ab a a a a a a b ab a a b a a a c a a b a a b b b ab a ab b ab a a [7,8 ], mgΠkg, mgΠkg,, [14 ] 019mgΠkg,,, 20 % 25 % [15 ],,,,,, [4 ] 312,,, 016mgΠkg,, 016mgΠkg,,,,, GOT GPT, mgΠkg, GSH2 px H 2 O 2, (ROOH),, ROOH ; [16 ], mgΠkg GSH2px,016mgΠkg,,,,,,, mgΠkg,,,, 019mgΠkg,,, 019mgΠkg,,, [17 ]

5 Journal of Nuclear Agricultural Sciences 2008,22 (2) : mgΠkg 016mgΠkg,,,, 0167mgΠkg : [ 1 ] Rotruck J T, Pope A L, Ganther H E, et al. Selenium : biochemical role as a component of glutaobione peroxidase. Science, 1973, 79 : [ 2 ].. :,1996 : [ 3 ] Serra A B, Serra S D, Shinchi K, et al. Bioavailability of rumen bacterial selenium in mice using tissue uptake technique. Biological trace element research, 1997, 58 (3) : [ 4 ] Henry P R, Ammerman C B. Selemium bioavailability in bioavailability of nutrents for animals, Amino Acids, Minerals and Vitamins C. New York :American Acidemin Press,1995 [ 5 ] Fisher D D. Comparative effects of inorganic and organic selenium sources (selenium yeast) on selenium status of lactating cows. Lyons T P, Jacques K A. ( eds. ). Biotechnology in the feed industry : Proceedings of Alltech s Eleventh Annual Symposium. Nottingham (United Kingdom) : Nottingham University Press, 1995 : [ 6 ],,,..,2007,21 (3) : [ 7 ],,..,2005,31 (4) : [ 8 ],,,..,2005,41 (9) :35 37 [ 9 ] Schrauzer G N. Selenomethionine : a review of its nutritional significance, metabolism and toxicity. Journal of nutrition, 2000, 130 (7) : [10 ].. : : [11 ],,..,2006,20 (5) : [12 ] Van Soest P J, Robertson J B, Lewis B A. Methods for dietary fiber, neutral detergent fiber and non2starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 1991, 74 (6) : [13 ] ( ). ( ). :, 2002 : 188,229,232 [ 14 ] Hidiroglou M, Heaney D P, Jenkins K J. Metabolism of inorganic selenium in rumen bacteria. Can Journal of Physioogy and Pharmacology, 1968, 46 (1), [15 ].. : : [16 ],,,..,1999,28 (1) : [17 ].. :, 1997, ( 241 ) [11 ],. DPS. :,2007 [12 ],,,.., 1999,25 (2) :l 3 [13 ] Kanno H, Hattori M, Sato A. Mating behaviour of the rice stem borer moth, Chilo suppressalis. Walker ( Lepidoptera : Pyralidae ). Appl Entomol Zool, 1980,15 (4) : [14 ] Urquijo C J L, Manzano GV. Mating disruption strategies for the control of pink bollworm ( Pectinophora gossypiella Saunders) in cotton fields. Bol Sanid Veg Plagas,2002,28 : [15 ] Boguslawski C V, Basedow T. Studies in cotton fields in Egypt on the effects of pheromone mating disruption on Pectinophora gossypiella ( Saund. ) ( Lepidoptera : Gelechiidae ), on the occurrence of other arthropods, and on yields. J Appl Entomol,2001,125 :

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