Effects of Hyriopsis cumingii Plague Virus on Digestive Organs of the H1 cumingii

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1 Chinese Journal of Zoology 2009,44 (3) :1 8 3 ( ; ) : d, ( Hyriopsis cumingii), ( H. cumingii Plague Virus,HcPV) :, 7 d,,7 d ;,,, :, 7 d,7 d,, ;,,,,, : ; ; ; ; :Q955 :A : (2009) Effects of Hyriopsis cumingii Plague Virus on Digestive Organs of the H1 cumingii XIAO Tiao2Yi LIU Qiao2Lin ZHANG Huai2Yun 3 GE Xi2Kai XU Bao2Hong SU Jian2Ming ZHONGLei ( College of Animal Science and Technology, Hunan Agricultural University, Changsha ; Central South University of Forestry and Technology, Changsha , China) Abstract :The pathological changes of digestive organs were observed by optical microscopy and electron microscopy at the 3rd, 5th, 7th, 9th, and 11th day after artificial infection of Hyriopsis cumingii with the pathological tissue liquid. The results showed that the H. cumingii Plague virus damaged the structure of the digestive organs. The liver was most seriously damaged. This digestive gland was swelling and its lumen was smaller at the 7th day of infection,and after that vacuolation and coenocytes were observed. Under electron microscope,the organelles such as mitochondria and endoplasmic reticulum were damaged and the virus particles were frequently observed. Pathological changes also included stomach and intestine damages. Their structure damage was not obvious within 7 days of infection,but after the 7th day the columnar cells became swellen and the cilia were detached from the epithelial cells. Under electron microscope, organelles damage and even vacuolation were observed. The virus particles became more frequently observed at later stage of infection,but less than observed in the liver. Key words : Hyriopsis cumingii ; H. cumingii Plague Virus ; Digest gland ; Enteron ; Histopathology (No. 06NK3049), (No. 07A012) ; 3,E2mail cs. hn. cn ;,, ; : ; E2mail com. cn : , :

2 2 Chinese Journal of Zoology 44 ( Hyriopsis cumingii) (Mollusca) (Lamellibranchia) ( Eulamellibranchia) (Unionidae), [1 ],, [2 ] 20 80, Bouin,, [3 8 ] ,, [9 ], : ( ), 50 %, - 20 :, cm, cm, cm,,,, , 3, ( ), ph 712 PBS 1 10, rπmin 30 min,, ( ) IUΠ ml,4 16 h, ( 10 ) ( 15 ), 015 mlπ PBS ; 015 mlπ, , d 3,, 4, 50 %, Bouin, ( 4 6 m),h. E,Motic %, 1 %,, Epon 812,, 2,J EM kv ( :1 6),,,, H. E, ; ( :1) 3 d,, ( :2) ; 5 d, ( :3) ; 7 d ( :4) ; 9 d,,, ( :5) ; 11 d,,,, ( :6) ( :7 12),,,,

3 3 : 3,,, ( :7), 3 d, ( :8) ; 5 d ( :9) ; 7 d, ( :10) ; 9 d, ( :11) ; 11 d, ( :12) ( :13 18),,,, ( :13) 3 d,, ( :14) ; 5 d, ( : 15) ; 7 d, ( :16) ; 9 d, ( :17) ; 11 d,,, ( :18) ( :19 24),,,, ( :19) ; 3 d,,, ( :20) ; 5 d ( :21),,, ( :22) ; 9 d, ( :23) ; 11 d,, ( :24) (, :25 30), ( :25) ; 3 d,, ( :26) ; 5 d, ( :27) ; 7 d,, ( :28) ; 9 d ( :29) ; 11 d ( :30) 3,,, [10,11 ], ( ) [12 16 ], [17 ] ( Argopectens irradias lamarck) ; [18 ] ; [19 ] ( Chlamys f arreri) ; [20 ] ; [21 ] ; [9 ],,, 311,, ( Ostrea talienwhanensis crosse ) [22 ] ( Solen gouldii) [23 ] ( Mytilus galloprovincialis) [24 ] [25 ] ( Meretrix meretrix) [26 ],,,,

4 4 Chinese Journal of Zoology 44, 3 d,,,, [9 ], [18 ], [27 ] ( ),, [18 ] [28 ], [29 ] ( Haliotis diversicolor) ( ),,,,,, [30 ],,,9 d,9, [31 ],,, 312, :,, 7 d,7 d, [27 ], :,,, ;,,,,, [32 ] ( ) ( ) ( ),, [33 ],,,,,,,,, d,,,,,,,,,,,,, ( ),,,,,

5 3 : 5, [34 ],,,,,, : 3 d, 5 d,, 7 d, d, [ 1 ],. ( ). :,1997, [ 2 ],,..,2000,12 (2) : [ 3 ]..,1986, 2 : [ 4 ]. I..,1986,26(4) : [ 5 ]...,l987,27 (2) : [ 6 ]..,1982, 5 : [ 7 ],,..,1984, (5) : [ 8 ],,..,1988, (324) : [ 9 ],,..,1995,19 (1) :1 71 [10 ] Thieltges D W, Krakau M,Andresen H, et al. Macroparasite community in molluscs of a tidal basin in the Wadden Sea. Helgoland Marine Research,2006,60 : [11 ] Sparague V. Comments on trends in research on parasitic disease of shellfish and fish. Marine Fisheries Review,1978,40 (10) : [12 ] Farley C A. Viruses and viruslike lesions in marine mollusks. Marine Fisheries Review,1978,40(10) : [13 ] Elston R. Virus2like particles associated with lesions in larval Pacific oysters ( Crassostrea gigas ). Journal of Invertebrate Pathology,1979,33 : [14 ] Elston R A, Wilkinson M T. Pathology, management and diagnosis of oyster velar virus disease (OVVD). Aquaculture, 1985,48 : [15 ] Azevedo C. Virus2like particles in Perkinsus atlanticus (Apicomplexa, Perkinsidae). Diseases of Aquatic Organisms, 1990,9 : [16 ] Comps M,Cochennec N. A herpes2like virus from the European oyster Ostrea edulis L. Journal of Invertebrate Pathology, 1993,62(2) : [17 ],,..,2004,28 (3) : [18 ],,..,2003,33(3) : [19 ],,..,2003, (7) : [20 ],..,2002, (1) : [21 ],,.. ( ),2001,27 (1) : [22 ],,.. :.. :,1999, [23 ],,..,2000,21 (2) : [24 ],,..,1999, 8( 1) : [25 ],,.. ( ),2001,14 (3) : [26 ],,..,2001, (2) : [27 ],,.. ( ), 2006,25(2) : [28 ],,..,2002,21 (4) : [29 ],,..,2007,31 (3) :28 321

6 6 Chinese Journal of Zoology 44 [30 ],.. ( ),1998,35 (1) : [31 ] Johnson M A, Paulet Y M, Donval A, et al. Histology, histochemistry and enzyme biochemistry in the digestive system of the endosymbiont bearing bivalve Loripes lucinalis (Lamarck ). Journal of Experimental Marine Biology and Ecology,1996,197 : [32 ],,.. ( ),1995,18(4) : [33 ],,..,1994, (5) :3471 [34 ],,..,1995,19 (2) : ,7 12,13 18, 400, = 50 m 11 ; 21 3 d ; 31 5 d ; 41 7 d ; 51 9 d ; d ; 71 ; 81 3 d ; 91 5 d ; d ; d ; d ; 131 ; d ; d ; d ; d ; d Explanation of Plate Microstructural pathological changes of digestive organs in the H. cumingii 1-6 are livers,7-12 are stomaches,and are midguts, 400,Bar = 50 m. 11Healthy mussels liver ; 21The liver 3 days after infection ; 31The liver 5 days after infection ; 41The liver 7 days after infection ; 51The liver 9 days after infection ; 61The liver 11 days after infection ; 71Healthy mussels stomach ; 81The stomach 3 days after infection ; 91The stomach 5 days after infection ; 101The stomach 7 days after infection ; 111The stomach 9 days after infection ; 121The stomach 11 days after infection ; 131Healthy mussels midgut ; 141The midgut 3 days after infection ; 151The midgut 5 days after infection ; 161The midgut 7 days after infection ; 171The midgut 9 days after infection ; 181The midgut 11 days after infection. GE. ; L. ; G. ; Va. ; EC. ; S. ; ML. ; MF. ; MC. ; CC. ; Pk. GE. Gland epithelium ; L.Lumen ; G. Gland ; Va. Vacuole ; EC. Epithelial cells ; S. Submucosa ; ML. Mucosal layer ; MF. Muscle fibers ; MC. Mucus cells ; CC. Columnar cells ; Pk. Polykaryon ,25 30, = 500 nm 191, ; d, ; d, ; d, ; d, ; d, ; 251, ; d, ; d, ; d, ; d, ; d, Explanation of Plate Ultrastructural pathological changes of digestive organs in the H. cumingii are livers, are visceral missions, Bar = 500 nm. 191Health mussels liver, ; 201The liver 3 days after infection, ; 211The liver 5 days after infection, ; 221The liver 7 days after infection, ; 231The liver 9 days after infection, ; 241The liver 11 days after infection, ; 251Health mussels visceral mission, ; 261The visceral mission 3 days after infection, ; 271The visceral mission 5 days after infection, ; 281The visceral mission 7 days after infection, ; 291The visceral mission 9 days after infection, ; 301The visceral mission 11 days after infection, ER. ; Cb. ; VP. ; Mi. ; Va. ; CT. ER. Endoplasmic reticulum ; Cb. Cytoblast ; VP. Virus particle ; Mi. Mitochondrion ; Va. Vacuole ; CT. Connective tissue.

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