ISSN 1007-7626 CN 11-3870 / Q http / / cjbmb bjmu edu cn Chinese Journal of Biochemistry and Molecular Biology 2011 3 27 3 212 ~ 217 * 100191 2 R589 2 Feedback Regulation of Adipocytokines in Proliferation and Differentiation of Adipocyte ZHOU Yan-Lan CHI Yu-Jing GUAN You-Fei YANG Ji-Chun * Department of Physiology and Pathophysiology Peking University Diabetes Center Peking University Health Science Center Beijing 100191 China Abstract Obesity has been demonstrated to be a causal factor of insulin resistance type 2 diabetes hypertension hyperlipidemia coronary heart disease and other metabolic diseases Obesity is generally indicated by abnormal increased number and mass of adipocytes leading to excessive deposition of lipids The proliferation and differentiation of preadipocyte is regulated by many factors including hormones nutrients and adipocytokines which are referred to the cytokines secreted by adipose tissues In animal models and a majority of patients with obesity abnormal profile of adipocytokines in the circulation and adipose tissues can be observed in association with the development and progression of obesity In the present paper the regulatory role of several important adipocytokines in adipocyte proliferation and differentiation are summarized and discussed Key words adipocytokines obesity adipocyte proliferation and differentiation 2 1 2 2 9 000 2010-11-19 2011-01-18 No 30771030 No 30870995 10 * Tel 010-82805027 E-mail yangj@ bjmu edu cn Received November 19 2010 Accepted January 18 2011 Supported by National Natural Science Foundation of China No 30771030 No 30870995 * Corresponding author 010-82805027 E-mail yangj@ bjmu edu cn
3 213 TNFα α Leptin Resistin TNF-α α TNF- Adiponectin Visfatin Chemerin TNF-α 1 α 2 α TNF-α Leptin 1994 TNF-α TNF-α TNF-α 9 TNF-α 2 10 TNF-α 2 TNF-α 1 TNF-α 2 3T3-L1 TNF-α TNF-α 1 3 TNF-α C / EBPδ PPARγ Leptin 4 Chae 5 LPL LPL JNK NF-kB TNF-α JNK SP600125 γ PGC-1a IkkB 2 UCP2 3 UCP3 NF-kB SP600125 TNF-α 12 IkkB NF-kB TNF-α PPARγ CEB-α PPAR-γ TNF-α NF-kB TNF-α 13 3T3-L1 GPDH 3T3-L1 TNF-α 3T3-L1 10 nmol / L ADRP perilipin ADRP 3T3-L1 14 Leptin PI3K / mtor perilipin 15 6 TNF-α Qian 7 TNF-α TNF-α Caspase-3 TNF-α 8 16 11 3T3-L1
214 27 2 Kim 17 COS 3T3- L1 Pref-1 C / EBPα 3T3-F442A PPAR-γ ap-2 FAS 80% Pref-1 C / EBPα 3T3-L1 Gong 18 5 3T3-L1 2005 Fukuhara 23 sirna 3T3-L1 8800 cdna Gu 19 3T3-L1 Visfatin Visfatin B pre-b-cell colonyenhancing factor PBEF 24 Nampt 25 Visfatin PBEF Nampt Fu 21 3T3- L1 3T3-L1 3 Resistin Bauche 22 COS Visfatin Fukuhara 23 Visfatin CD36 PPARγ C / EBPα FAS 20 3T3-L1 3T3- F442A 4 Visfatin LPL PPARγ ap2 FAS Adiponectin 26 Visfatin 10
3 215 Chemerin-Chemerin Chemerin Chemerin Ca 2 + ERK1 / 6 Chemerin 2 Chemerin Chemerin 2007 ERK1 /2 4 d Chemerin CHEMERIN CHEMERIN PPARγ 27 Chemerin-Chemerin Chemerin 3T3-L1 3 d 28 Muruganandan 29 Chemerin mrna 8 d Chemerin Chemerin mrna CHEMERIN Chemerin-Chemerin Chemerin RNAi Chemerin 3T3-L1 7 8 d Chemerin / Chemerin Chemerin 8 d Leptin Perilipin FAS GLUL4 HSL Resistin Visfatin Chemerin Adiponectin Chemerin PPARγ Adiponectin Fig 1 The feedback regulatory role of adipocytokines in the proliferation and differentiation of adipocyte In overnutrient conditions the disturbed balance in pro-differential and anti-differential adipocytokines play crucial roles in the development and progression of obesity
216 27 in obese rats J Clin Invest Med 1998 21 2 54-62 11 Ambati S Kim H K Yang J Y et al apoptosis and adipogenesis in 3T3-L1 adipocytes J Pharmacol 2007 73 3 378-384 12 Fig 1 mrna J Gui-Fen Wen Xu-Hui References 1 metabolism and gene expression of differentiation-associated J Cheng Hong Ding growth factors and transcription factors during differentiation and Xiu-Yuan Zhang Mei-Xian et al Relationship between maturation of 3T3-L1 preadipocytes J Endocr J 2008 55 adipocytokines and adiposity in children and adolescents J 5 827-837 Chin J Pract Pediatr 2010 25 4 271-274 2 / inhibition of the insulin-stimulated increase in fatty acid uptake in J Zhang Jun Chu Zhi-Hua differentiated 3T3-L1 adipocytes J Metabolism 2006 55 1 Lu Huan et al Compared the adipocyte factors concentration level of rat in obesity / diabetes process J Agric Reclam Med 2009 31 1 7-10 3 Xu H Sethi J K Hotamisligil G S Transmembrane tumor necrosis factor TNF -alpha inhibits adipocyte differentiation by selectively activating TNF receptor 1 J J Biol Chem 1999 274 37 26287-26295 4 Kudo M Sugawara A Uruno A et al Transcription suppression of peroxis proliferator-activated receptor gamma2 gene expression by tumor necrosis factor alpha via an inhibition of CCAAT / enhancer-binding protein delta during the early stage of adipocyte differentiation J Endocrinology 2004 145 11 4948-4956 5 Chae G N Kwak S J NF-kappaB is involved in the TNF-alpha induced inhibition of the differentiation of 3T3-L1 cells by reducing PPARgamma expression J Exp Mol Med 2003 35 5 431-437 6 -α Yi Chuan Xue Za Zhi 2007 24 3 251-255 J 20 Xu W Yu L Zhou W et al Resistin increases lipid Liu Mei-Fang Zu Lu-Xia Xu Chong et al Tumor necrosis factor-α upregulates the protein levels of adipose differentiation-related protein in rat differentiated adipocytes J J Peking Univ Health Sci 2008 40 6 578-582 7 Qian H Hausman D B Compton M M et al TNFalpha induces and insulin inhibits caspase 3-dependent adipocyte apoptosis J Biochem Biophys Res Commun 2001 284 5 1176-1183 8 Zhang H H Kumar S Barnett A H et al Dexamethasone inhibits tumor necrosis alpha-induced apoptosis and interleukin-1 beta release in human subcutaneous adipocytes and preadipocytes J J Clin Endocrinol Metab 2001 86 6 2817-2825 9 Campfield L A Smith J Burn P The OB protein leptin pathway--a link between adipose tissue mass and central neural networks J Horm Metab Res 1996 28 12 619-632 10 Shillabeer G Vydelingum S Hatch G et al Long-term regulation of leptin expression is correlated with adipocyte number Effects of leptin on Biochem Leptin PGC-1 alpha UCPs mrna UCPs Luo Yang Gong-She Role of leptin in porcine preadipocyte pifferentiation and mrna expression of PGC-1α and UCPs J Chin J Biochem Mol Biol 2007 23 1 51-55 13 Kim W K Lee C Y Kang M S et al Effects of leptin on lipid 14 Ho M Foxall S Higginbottom M et al Leptin-mediated 8-12 15 Maya-Monteiro C M Almeida P E D Avila H et al Leptin induces macrophage lipid body formation by a phosphatidylinositol 3-kinase-and mammalian target of rapamycin-dependent mechanism J J Biol Chem 2008 283 4 2203-2210 16 Palou A Pico C Leptin intake during lactation prevents obesity and affects food intake and food preferences in later life J Appetite 2009 52 1 249-252 17 Kim K H Lee K Moon Y S et al A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation J J Biol Chem 2001 276 14 11252-11256 18 Gong H Ni Y Guo X et al Resistin promotes 3T3-L1 preadipocyte differentiation J Eur J Endocrinol 2004 150 6 885-892 19 Gu N Guo X R Ni Y H et al Overexpression of resistin affect 3T3-L1 adipocyte lipid metabolism J Zhonghua Yi Xue accumulation and CD36 expression in human macrophages J Biochem Biophys Res Commun 2006 351 2 376-382 21 Fu Y Luo N Klein R L et al Adiponectin promotes adipocyte differentiation insulin sensitivity and lipid accumulation J J Lipid Res 2005 46 7 1369-1379 22 Bauche I B El Mkadem S A Pottier A M et al Overexpression of adiponectin targeted to adipose tissue in transgenic mice impaired adipocyte differentiation J Endocrinology 2007 148 4 1539-1549 23 Fukuhara A Matsuda M Nishizawa M et al Visfatin a protein secreted by visceral fat that mimics the effects of insulin J Science 2005 307 5708 426-430 24 Samal B Sun Y Stearns G et al Cloning and characterization of the cdna encoding a novel human pre-b-cell colony-enhancing factor J Mol Cell Biol 1994 14 2 1431-1437
3 217 25 Rongvaux A Shea R J Mulks M H et al Pre-B-cell colonyenhancing factor whose expression is up-regulated in activated lymphocytes is a nicotinamide phosphoribosyl transferase a cytosolic enzyme involved in NAD biosynthesis J Eur J Immunol 2002 32 11 3225-3234 26 Yang C C Deng S J Hsu C C et al Visfatin regulates genes related to lipid metabolism in porcine adipocytes J J Anim Sci 2010 88 10 3233-3241 27 Roh S G Song S H Choi K C et al Chemerin--a new adipokine that modulates adipogenesis via its own receptor J Biochem Biophys Res Commun 2007 362 4 1013-1018 28 Goralski B McCarthy T C Hanniman E A et al Chemerin a novel adipokine that regulates adipogenesis and adipocyte metabolism J J Biol Chem 2007 282 38 28175-28188 29 Muruganandan S Roman A A Sinal C J Role of chemerin / CMKLR1 signaling in adipogenesis and osteoblastogenesis of bone marrow stem cells J J Bone Miner Res 2010 25 2 222-234 2011-1 2 ISBN 978-7-03-030126-0 260 00 16 2011 1 010-64031535 email zhouwenyu@ mail sciencep com http / / shop sciencepress cn www lifescience com cn email lifescience@ mail sciencep com 010-64012501