Human amniotic epithelial cells and embryonic stem cells

22 1 2010 1 Chinese Bulletin of Life Sciences Vol. 22, No. 1 Jan., 2010 1004-0374(2010)01-0064-05 1 200030 2 201203 (MEF) (HAEC) DNA Q813 A Human amniotic epithelial cells and embryonic stem cells LAI Dong-mei 1 *, GUO Li-he 2 (1 The International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University, Shanghai 200030, China; 2 Hehong (Shanghai) Biotechnology Ltd, Shanghai 201203, China) Abstract: Human embryonic stem cells have great prospects for medical applications, but the growth of human embryonic stem cells requires high quality of culturing conditions in vitro and it is difficult to simulate in vivo environment, therefore the control of human embryonic stem cells growth is often not ideal. The use of mouse embryonic fibroblasts (MEF) as feeder layer exists the problem of pollution of animal pathogens. In this paper, the characteristics of human amniotic epithelial cells (HAEC) and its use as a feeder layer of embryonic stem cells are described. The genomic DNA methylation in embryonic stem cell differentiation is also explored. Optimization and standardization of culture methods is needed for research as well as for clinical purpose. Key words: human amniotic epithelial cells; embryonic stem cells; epigenetic modification (human embryonic stem cells, HES ) HES (1) (2) HES HES [1] 2009-06-19 2009-08-17 2007 (07pj4090) * E-mail:Laidm2003@hotmail.com Tel 15900897917

65 HES HES HES HES [2] 1 (murine embryonic fibroblasts MEF) STO γ C MEF HES [3] HES MEF MEF (fibroblast growth factor, FGF) (leukemia inhibitory factor, LIF) [4] MEF HES (1) HES MEF HES (2) MEF MEF (3) HES (4) MEF HES MEF MEF HES [5-8] HES HES [9] HES HES [10] HES 2 (amnion) 0.02~0.5 mm (amniotic epithetical cells AEC) 2 m 2 2 20 (1) (2) EGF KGF HGF bfgf BDNF NT-3 NGF (3) (4) [11] (human amniotic cell HAE) (human amniotic epithelia cell, HAEC) Miyamoto [8] Ito [12] HAEC CMK6 Chen [13] HAEC

66 MEF HAEC 3~5 (HAECs ) LIF LIF MEF [14] HAEC ES Oct-4 Nanog Sox-2 Rex FGF TERT MEF ES HAECs ES 20 HAEC LIF LIF [15] HES HES (1) Oct-4 Nanog Sox-2 (2) (3) ES HES [16,17] 3 DNA DNA DNA DNA DNA CpG 5' Oct-4 Nanog (self-renewal) (pluripotency) [18,19] Nanog Oct-4 Nanog OcT-3/4 Nanog ES Oct-4 Nanog Sox2 Stat3 HES [20,21] Nanog ANTP, NK 12 12p13.31 7.84 Mb Nanog cdna 2 184 305 3 homeodomain DNA W Oct-4/ Sox-2 FoxD3 Nanog -180-270 bp Nanog [18] Darr [22], Nanog ES Oct-4 POU 6 6p21.31 Nanog Oct-4 HES (FGF-4 ) 1 (Utf-1) zfp42/rex-1 Oct-4 Sox2 c-myc Klf4 ES [23, 24] Oct-4 (ES ) (EC ) (EG ) Oct-4 [25] RA NT2 NT2 Oct-4 RA OTF9-63 Oct-4 1.3 kb DNA [26] Deb-Rinker [27] RA NT2 Nanog Oct-4 RA 8 Nanog ( OcT-4 Sox2 DNA ) Oct-4 5' Freberg [28] (NCCIT) 293T 1 h 293T Oct-4

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