Progress in Studies of Collagen

ISSN 100727626 CN 1123870ΠQ 2001 12 Chinese Journal of Biochemistry and Molecular Biology 17 (6) :675 679 3 ( 130024) (collagen ) C Q249 Progress in Studies of Collagen CHEN Ting LU Jun ZHANGJing LI Yu2xin 3 HUANG Bai2qu ( Institute of Genetics and Cytology Northeast Normal University Changchun 130024 China) Abstract Collagen a new member of the collagen family has been classified as the subclass which col2 lagen belongs to It plays an important role in adhering basement membrane to the underlying connective tissue stroma Some results suggest that it is involved in the invasive process of carcinomas and it may provide a sensitive indicator of tumor invasion Recently restin a C2terminal fragment of the collagen has been reported as an angiogenesis inhibitor which shares homology with endostatin The gene structure and organiza2 tion tissue distribution and the activity of collagen were discussed Key words collagen molecular structure gene location tissue distribution bioactivity Gly2X2Y (collagens forming sheets) [1 ] Gly2X2Y 3 1 ( ) 2 ( ) 3 ( ) ( ) ( beaded fila2 [2 ] 19 20 ments) [3 19 ] (anchoring fibrils) (protein with multiple triple2helix domains and interrup2 tions multiplexins) [5 ] :2001204216 :2001207216 (973 ) (No G1999053902) 3 Tel : (0431) 5685085293502 Fax : (0431) 5687517 [4 ] 1974 1 (fibril associated collagens with interrupted tri2 Received :April 16 2001 ;Accepted :July 16 2001 Supported by a Major State Basic Research Program ( 973 Program ) Fund ple helices FACITs) (No G1999053902) Partially 3 Corresponding author Tel : (0431) 5685085293502 Fax : (0431) 5687517

676 17 SDS2PAGE 1 [7 ] cdna 45 % [6 ] 1388 ( Fig ( 1) 9 1) N 25 10 7 [7 ] 530 1 COL1 [9 ] C (NC1) ( thrombospondin NC10 NC11 [8 ] [10 577 9 ] (COL1 COL9) 8 16 % (NC2 NC9) 15 114 30 % 61 % [10 ] 183 [7 7 45 ] C 256 [11 (NC10) 4 ] NC10 NC11 Cys [7 ] ( Fig 1) [9 ] NC1 motifs) 2 Cys (179 235) 11 2Ser2Gly ( Fig 1) 46 % 181 36 [9 Kivirikko ( 1) 91 ] 1411922 kd [7 ] Myers N2 6 ( 1) 125 kd [6 ] Kivirikko 8 [8 ] Fig 1 The molecular structure of human collagen and mouse collagen (A) Human collagen ; (B) Mouse collagen Signal piptide ; collagen domain ; non2collagen domain ; tsp :thrombospondin 2 55 kb [12 ] 2 5 11 145 kb 42 5 90 kb 41 1 41 36 N 12 42 bp 548 bp 89 bp 55 kb 55 kb 12 36

6 : 677 [14 8 ] 16 17 20 23 24 4 26 27 32 4 36 bp 1 54 bp 2 63 bp 1 81 bp 32 411 37 42 C [15 N C ] Eklund [12 ] Hagg 3 + 1A [18 44 45 ] TT [12 ] - 398-142 TATAA - 474 ATG 4 SP1 G+ C(68 4 %) Huebner [13 9q212q22 ] 21 21q22 3 [14 10 ] 3 [15 16 ] [20 Kivirikko ] C NC11 Northern [21 ; ] mrna [21 ] ; Lewis mrna [16 ] Hagg [22 ] 1 C [17 ] NC10 C NC11 NC10 C [20 ] Col15 ( 1) [19 ] 412 [4 ] : 1999 John

678 17 20 995 19 904 16 373 [11 ] Western blot C (NC10) (related to endostatin restin) Ram2 chandran res2 tin 185 22 kd restin 78620 restin 30 % restin [23 ] 2000 Sasaki restin NC10 VEGF FGF22 NC10 FGF22 restin NC10 NC10 ( ) [10 ] 220 224 ( References) 1 Eastoe J E Composition of collagen and allied proteins In :Ramachan2 dran G N ed Treatise on CollagenLondon : Academic 1967 1 72 2 Van der Rest M Garrone R Collagen family of proteins FAS EB J 1991 5 :1814 1823 3 Steinert P M Roop D R Molecular and cellular biology of intermediate filaments Annu Rev Biochem 1988 57 :593 625 4 Rehn M Pihlajaniemi T 1 ( ) a collagen chain with frequent in2 terruptions in the collagenous sequence a distinct tissue distribution and homology with type collagen Proc Natl Acad Sci USA 1994 91 : 4234 4238 5 Oh S P Kamagata Y Muragaki Y Timmons S Ooshima A Olsen B R Isolation and sequencing of cdnas for proteins with multiple domains of Gly2Xaa2Yaa repeats identify a distinct family of collagenous proteins Proc Natl Acad Sci USA 1994 91 :4229 4233 6 Myers J C Kivirikko S Gordon M K Dion A S Pinhlajaniemi T Identification of a previously unknown human collagen chain 1 ( ) characterized by extensive interruptions in the triple2helical region Proc Natl Acad Sci USA 1992 89 :10144 10148 7 Kivirikko S Heinamaki P Rehn M Honkanen N Myers J C Pihla2 janiemi T Primary structure of the 1 chain of human type collagen and exon2intron organization in the 3 region of the corresponding gene J Biol Chem 1994 269 (7) :4773 4779 8 Muragaki Y Abe N Ninomiya YOlsen B R Ooshima A The human 1 ( ) collagen chain contains a large amino2terminal non2triple heli2 cal domain with a tandem repeat structure and homology to 1 ( ) collagen J Biol Chem 1994 269 (6) : 4042 4046 9 Rehn M Hintikka E Pihlajaniemi T Primary structure of the 1 chain of mouse type collagen partial structure of the corresponding gene and comparision of the 1 ( ) chain with its homologue the 1 ( ) collagen chain J Biol Chem 1994 269 (19) : 13929 13935 10 Sasaki T Larsson H Tisi D Claesson - Welsh L Hohenester E Tim2 pl R Endostatins derived from collagens and differ in struc2 tural and binding properties tissue distribution and anti2angiogenic ac2 tivity J Mol Biol 2000 301(5) :1179 1190 11 John H Preissner K T Forssmann W G Standker L Novel glycosylat2 ed forms of human plasma endostatin and circulating endostaitin2related fragments of collagen Biochemistry 1999 38 (32) : 10217 10224 12 Hagg P M Muona A Lietard J Kivirikko S Pihlajaniemi T Complete exon2intron organization of the human gene for the 1 chain of type collagen ( COL15A1) and comparison with the homologous COL18 1 Gene J Biol Chem 1998 273(28) :17824 17831 13 Huebner K Cannizaro L A Jabs E W Kivirikko S Manzone H Pihlajaniemi T Myers J C Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 q22 Genomics 1992 14 : 14 Oh S P Warman M L Seldin M F Cheng S D Knoll J M Timmons S Olsen B R Cloning of cdna and genomic DNA encoding human type collagen and localization of the 1 ( ) collagen gene to mouse chromosome 10 and human chromosome 21 Genomics 1994 19 (3) : 494 499 15 Myers J C Dion A S Abraham V Amenta P S Type collagen exhibits a widespread distribution in human tissues but a distinct local2 ization in basement membrane zones Cell Tissue Res 1996 286 (3) : 493 505 16 Kivirikko S Saarela J Myers J C Autio2Harmainen H Pihlajaniemi T Distribution of type collagen transcripts in human tissue and their production by muscle cells and fibroblasts Am J Pathol 1995 147(5) :1500 1509 17 Hagg P M Hagg P O Peltonen S Autio2Harmainen H Pihlajaniemi T Location of type collagen in human tissue and its accumulation in the interstitial matrix of the fibrotic kidney Am J Pathol 1997 150 (6) :2075 2086 18 Eklund L Piuhola J Komulainen J Sormunen R Ongvarrasopone C Fassler R Muona A Ilves M Ruskoaho H Takala T E S Pihlajanie2 mi T Lack of type collagen causes a skeletal myopathy and cardio2

6 : 679 vascular defects in mice Proc Natl Acad Sci USA 2001 98 (3) :1194 1199 19 Amenta P S Briggs K Xu KGamboa E Jukkola A F Li D Myers J C Type collagen in human colonic adenocarcinomas has a different distribution than other basement membrane zone proteins Hum Pathol 2000 31(3) :359 366 20 Hanabal M D Volk R Ramchandran R Simons M Sukhatme V P Cloning expression and in vitro activity of human endostatin Biochem Biophys Res Commun 1999 258 :345 352 21 O Reilly M S Boehm T Shing Y Fukai N Vasios G Lane W S Flynn E Birkhead J R Olsen B R Folkmen J Endostatin an endoge2 nous inhibitor of angiogenesis and tumor growth Cell 1997b 88 (2) : 277 285 22 Boehm T Folkman J Browder T O Reilly M S Antiangiogenic thera2 py of experimental cancer does not induce acquired drug resistance Nature 1997 390 (6658) :404 407 23 Ramchandran R Dhanabal M Volk R Waterman MJ F Segal M Lu H Knebelmann B Sukhatme V P Antiangiogenic activity of restin NC10 domain of human collagen :Comparison to endostatin Biochem Bio2 phys Res Commun 1999 255 :735 739 : : : : : ( ) : : ( ) : ( ) 3 3 3 : 3 3 3 3 : 3 : 3 : 3 : 3 : ( ) : 320 :200031