31 1 2010 1 ENVIRONMENTAL SCIENCE Vol. 31,No. 1 Jan.,2010, 3, (,, 100084) :,.,, ( Microcystis aeruginosa),3 (A 2 O ) 10 6 ml - 1,> 0139 d - 1. A 2 O222,. TP ( K max ) ( R max ), Monod. :; ; ; ; :X173 :A :025023301 (2010) 0120076206 Gro wth Properties of Typical Water Bloom Algae in Reclaimed Water YANGJia, HU Hong2ying, LI Xin ( ESPC State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China) Abstract :Based on Algal Growth Potential Test, the water bloom risk of reclaimed water was studied. Microcystis aeruginosa was proved as the main algae under the experimental conditions in this study. In 3 kinds of secondary effluent (A 2 O, Oxidation Ditch and Activated Sludge), its maximum density could attain above 10 6 cells ml - 1, and the specific rate was above 0139 d - 1. Advanced treatment (A 2 O2Ultrafiltration Membrance Filter2Activated Carbon and Chlorine Disinfection) did not reduce the growth potential of Microcystis aeruginosa. The effects of initial concentration of phosphorus in reclaimed water on maximum density( K max ) and population growth rate ( R max ) of Microcystis aeruginosa can be described with Monod equation. Key words : Microcystis aeruginosa ; reclaimed water ; initial TP concentration ; algae growth potential ; water bloom control, [1,2 ]., [3 ]., [4,5 ], [6 8], [9 10].., TNΠmg L - 1,, TP. 1 111 11111 1 Q J G(Q J G), 1.. Table 1 Wastewater samples and related water quality TPΠmg L - 1 NH 4 + 2NΠmg L - 1 Q A 2 O 1515 111 0105 0101 215 0101 717 012 J 1617 112 0108 0112 213 0151 716 011 G 2711 214 219 0142 13 215 718 012 ph 11112 ( Microcystis aeruginosa) ( Selenastrum capricornutum) ( Scenedesmus dimorphus) ( Chlorella ellipsoidea). :2009202210 ; :2009206202 : (50825801) :(1983 ),,,,E2mail : yangjia1121 @gmail. com 3,E2mail :hyhu @tsinghua. edu. cn
1 : 77 BG11, SE. 25, 40 60 lx, 75 %, 14 h 10 h. 112 11211 (Algal Growth Potential Test) [11 ]. 250 ml 100 ml, (121, 30 min). 110 ml ( 15 mg L - 1 2 ), 25, 40 60 lx, 75 %, 14 h 10 h. 3.,., 105. 11212,TN TP NH + 4 2N 2 [12 ]. ph SG2 ph. 113 Logistic. Logistic [13 ] : N = dn dt K 1 + e a- rt (1) = rn K - N K, N t ( ml - 1 ) ; t (2) (d) ; K ( ml - 1 ) ; a, ; r (d - 1 ), ; dnπdt [ (ml d) - 1 ]. (1), S. (2),,, R max = rkπ4 [ (ml d) - 1 ]. 2 211 200 ml Q J G 5 ml, 4 10 23 28 30 38. 15 d, 1. 23 28,Q J G, 23 28. :15 d 1 Q J G Fig. 1 Growth potential of mixed algae in samples Q,J and G under different temperature, 2., ( Microcystis aeruginosa ) ( Chlorella ellipsoidea) ( Scenedesmus dimorphus) ( Selenastrum capricornutum).,4 Q, 2.,, 2 Q J G Table 2 Main species of algae in samples Q, J and G Π Q J G 4 10 23 28 ; ; ; 30 38 ;
78 31,. 3., 25, 3.,Q J G, 14 d 10 100.,G 3 d, ( 13 d) 111 10 7 ml - 1. Q J 5 d, ( 12 d) 216 10 6 ml - 1 215 10 6 ml - 1. 2 Q Fig. 2 Growth curve of mixed algae in sample Q,,,. [14 ],. [15 ],. [16 ],. 212 3 Q J G Fig. 3 Growth curve of Microcystis aeruginosa in samples Q, J and G Logistic Q J G, 3., Q J G 0139 0140 0155 d - 1,216 10 6 215 10 6 111 10 7 ml - 1. 3 Q J G Logistic Table 3 Logistic parameters of Microcystis aeruginosa s growth in samples Q,J,G R K ( r) 10 6 Π ml - 1 Πd - 1 R max 10 6 Π (ml d) - 1 Q 0198 2164 0159 0139 0126 0106 J 0197 2147 0188 0140 0125 0109 G 0198 10163 2138 0155 1145 0123, [17 ], TP 01018 118 mg L - 1, 01253 01345 d - 1,, Q J G.. 10 4 ml - 1 [18,19 ], 10 6 ml - 1 [9,10 ]. A 2 O,3 10 6 ml - 1,. 213,., 3
1 : 79., A 2 O + + +. 4 A 2 O222., 4., 3,10 d 10 6 ml - 1. 4 Table 4 Effluent qualities of different units of advanced treatment processes A 2 O( ) TNΠmg L - 1 TPΠmg L - 1 NH 4 + 2NΠmg L - 1 1515 111 0105 0101 215 0101 717 012 1614 012 0105 0101 214 0105 714 015 1312 114 0103 0102 212 0102 718 011 1216 118 0104 0101 212 0108 716 014 ph 4 Fig. 4 Growth potential of Microcystis aeruginosa in effluents of different advanced treatment processes 5 TP Fig. 5 Growth curves of Microcystis aeruginosa under different initial TP concentrations 214 TP N P., > 712, [20 23 ].,G Q J 913 310 209,. TP Q, TP ; N, TN 1510 mg L - 1. 5 TP.,TP,,., TP 011 012 015 mg L - 1, 4 d,315 10 6 414 10 6 811 10 6 ml - 1 ; TP 110 210 mg L - 1, 2 d, 912 10 6 ml - 1 110 10 7 ml - 1. Logistic TP, 6 7. TP 6 TP Fig. 6 Specific growth rate of Microcystis aeruginosa under different initial TP concentrations
80 31 7 TP Fig. 7 Maximum algal density and maximum growth rate of Microcystis aeruginosa under different initial TP concentrations,0181 0194 d - 1. TP ; ( R max = rkπ4), Monod. (3) TP Monod : R max,p = R max,p P (3) K P + P, R max,p, TP [ (ml d) - 1 ] ; P TP (mg L - 1 ) ; K P TP. (4) TP Monod : K max,p = K max,p P (4) K P + P, K max,p, TP ( ml - 1 ) ; K P TP. Monod TP, 5, : R max,p K max,p = 217 106 P 0130 + P = 111 107 P 0123 + P, 5 TP Monod Table 5 Monod parameters of the maximum growth rate and maximum algal density of Microcystis aeruginosa Monod Monod R max Π (ml d) - 1 K P R K Π ml - 1 K P R 217 10 6 0130 0198 111 10 7 0123 0197, TP. Monod, TP, TP,.,TP 0123 mg L - 1. Monod TP,. 10 6 ml - 1, TP 01023 mg L - 1. 3 ( 1 ), ( Microcystis aeruginosa),3 (A 2 O ) 10 6 ml - 1, 0139 d - 1,. (2) A 2 O222,. (3) TP ( K max ) ( R max ), Monod. (4) Monod, TN 15 mg L - 1,TP 0123 mg L - 1 TP. 10 6 ml - 1, TP 01023 mg L - 1.
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