27 6 2009 12-665- [ ] 1000-1182 2009 06-0665-04 Notch1 1 2 3 4 (1., 310015; 2., 310008; 3. ; 4., 310006) [ ] TSCC Notch1 EGFR 41 TSCC 39 LP 7 Notch1 EGFR LP Notch1 EGFR TSCC Notch1 EGFR Notch1 TSCC EGFR Notch1 TSCC [ ] Notch1 [ ] R 739.8 [ ] A [doi] 10.3969/j.issn.1000-1182.2009.06.022 Expression of Notch1 and epidermal growth factor receptor in human tongue squamous cell carcinoma and precancerous lesion HUANG Hong-jie 1, PING Fei-yun 2, HU Ji-an 3, ZHAO Shi-fang 4. 1. Dept. of Stomatology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, China; 2. Dept. of Stomatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310008, China; 3. Dept. of Oral Pathology, The Affiliated Stomatological Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China; 4. Dept. of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China [Abstract] Objective To investigate the expression of Notch1 in human tongue squamous carcinoma TSCC and precancerous lesion, and to explore the potential relation between Notch1 and epidermal growth factor receptor EGFR. Methods The expression of Notch1 and EGFR was detected in human TSCC n=41, tongue leukoplakia LP n=39 and normal tongue mucosa n=7 by immunohistochemistry. Results In normal tongue mucosa and LP, the positive staining of Notch1 was mainly distributed in stratum corneum, partially in stratum granulosum and stratum spinosum, but not in stratum basale, while the positive staining of EGFR was mainly distributed in stratum basale, rarely in stratum spinosum, but not in stratum granulosum and stratum corneum. In TSCC, Notch1 expression was mainly distributed in locations of squamous metaplasia, but not in peripheral cells of carcinomas, while EGFR expression was detected mainly in peripheral cells of carcinomas, but not in locations of squamous metaplasia. Conclusion Notch1 promotes the differentiation of epithelial cells in tongue mucosa and acts as a tumor suppressor in TSCC. EGFR may act as a negative regulator of Notch1 expression in epithelium of tongue mucosa and TSCC, for maintaining cell proliferation and promoting the tumorigenesis and progression of TSCC. [Key words] factor receptor tongue squamous cell carcinoma precancerous lesion leukoplakia Notch1 epidermal growth [ ] 2008-12-27 [ ] 2009-02-27 [ ] Y206192 20061057 [ ] 1968 [ ] Tel 0571-87217218 Notch - [1-2]
-666-27 6 2009 12 Notch 4 Notch1-4 5 min 37 Notch1 30 min PBS 3 5 min DAB 3% [1-2] tongue squamous cell carcinoma TSCC Notch1 1.2.3 PBS Santa Cruz epidermal growth factor receptor, EGFR TSCC [3] [4] Notch1 EGFR Notch1 EGFR EGFR TSCC 2 TSCC Notch1 TSCC 1.3 1 10 10 LP TSCC Notch1 EGFR 1.1 41 TSCC 39 leukoplakia 掊 2 P<0.05 LP 2000 9 2006 6 2 TSCC 27 2.1 Notch1 EGFR 14 32~76 59.4 LP 23 16 28~73 52.7 1997 Notch1 EGFR TSCC 19 15 7 34 16 LP 13 5 11 8 Notch1 7 7 10% 4 μm P<0.05 1 1 2 5 1.2 Notch1 1.2.1 Notch1 sc- P>0.05 1 6014 EGFR sc-03 Santa Cruz Biotechnology 1 75 1 100 SP 1.2.2 SP phosphate buffered saline PBS ph= 7.4 3 5 min 0.1 mol L -1 EDTA ph=9.0 PBS 3%H 2 O 2 10 min PBS 3 5 min 10% min 4 PBS 3 5 min 37 30 min PBS 3 Notch1 EGFR 5 40 10 7 39 LP 1 Notch1 SP 400 Fig 1 Notch1 expression in human normal tongue mucosa SP 400
27 6 2009 12-667- Tab 1 1 Notch1 EGFR The expression of Notch1 and EGFR in human normal tongue mucosa and leukoplakia Notch1 /% EGFR /% 7 75.3±17.1 31.9±8.1 14.3±3.6 0 0 0 2.7±0.6 20.7±10.4 13 73.7±16.9 30.5±8.9 13.2±2.9 0 0 0 4.6±1.2 33.5±13.2 11 74.1±17.3 31.1±7.2 12.8±3.0 0 0 0 8.2±2.3 47.3±12.7 8 72.9±18.1 30.3±9.2 13.9±3.7 0 0 0 11.7±3.0 61.0±15.1 7 73.2±16.3 30.6±8.7 14.2±3.3 0 0 0 15.9±4.1 78.3±16.9 Notch1 P<0.05 Notch1 7 TSCC Notch1 5 2 34 16 TSCC Notch1 Notch1 2 Notch1 SP 200 Fig 2 Notch1 expression in human tongue LP SP 200 5 EGFR EGFR 3 4 5 EGFR P<0.05 1 4 EGFR SP 200 Fig 4 EGFR expression in human tongue LP SP 200 3 EGFR SP 400 Fig 3 EGFR expression in human normal tongue mucosa SP 400 2.2 TSCC Notch1 EGFR Fig 5 Notch1 expression in human TSCC SP 200 41 TSCC 41 TSCC EGFR Notch1 EGFR Notch1 Notch1 19 15 TSCC 6 5 Notch1 TSCC SP 200
-668-27 6 2009 12 [2] [7-8] TSCC Notch1 P<0.05 2 2 Notch1 EGFR [9] TSCC LP Tab 2 The expression of Notch1 and EGFR in [10] [8] human TSCC Notch1 /% EGFR /% 19 23.1±7.0 24.3±6.8 15 12.5±3.9 45.7±7.9 7 0 68.1±13.4 6 EGFR TSCC SP 200 Fig 6 EGFR expression in human TSCC SP 200 Notch1 Notch1 TSCC Notch1 Notch1 TSCC Notch1 Notch1 TSCC Notch1 Notch1 Notch1 TSCC [11] Notch1 TSCC Tca8113 Notch1 [4] Notch1 EGFR 3 TSCC EGFR Notch [12] [13] [1-2 5] Notch1 TSCC [3] EGFR EGFR LP [6] [3 12] TSCC EGFR Notch1 Notch1 EGFR [7] Notch1 Notch1 [14] EGFR Notch1 [8] Notch1 Notch1 Notch1 [8] EGFR Notch1 TSCC EGFR Notch1 [7-8] TSCC LP Notch1 Notch EGFR [2 15] Notch1 EGFR TSCC Notch1 Notch1 [ ] [1] Weng AP, Aster JC. Multiple niches for Notch in cancer Context is everything[j]. Curr Opin Genet Dev, 2004, 14 1 48-54. [9] Notch1 [2] Miele L. Notch signaling[j]. Clin Cancer Res, 2006, 12 4 1074-1079. Notch1 672
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