Satoshi MITARAI 2 1, 2 1 3 1ml 10,000 40 80 4 Polymerase Chain Reaction PCR 5, 6 PCR Realtime PCR Transcription-Reverse Transcription Concerted reaction TRC 50 70 7 9 1 3 3 Gene Xpert Cepheid, USA TB-LAMP Gene Xpert 0204-8533 3-1- 24 Professor/Head, Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA) (3-1-24, Matsuyama, Kiyose-shi, Tokyo)
DNA FR -MTB TMA Hybridization protection assay HPA 5 TaqMan MTB Real-time PCR TaqMan probe 3 TRC Rapid M. TB TRC INAF probe, M. avium complex, M. kansasii 1.5 GENECUBE PCR QProbe, M. avium complex 50 Loopamp LAMP 50 Xpert MTB Rif 2 RFP rpob Real-time PCR Molecular Beacon 98 95 CI, 97-99 68 95 CI, 59-75 RFP 94 95 CI, 87-97 98 95 CI, 97-99 10 11 TB-LAMP PURE Procedure for Ultra Rapid Extraction, 1 Isothermal 9 DNA-DNA hybridization DDH, mg 16S rrna 99 12 SPEED-OLIGO MYCOBACT- ERIA Vircell, Spain PCR 5 19 13 GenoType Mycobacterium CM AS Hain Lifescience, Germany 31 5 14 M. tuberculosis M. bovis M. africanum M. canetti M. caprae M. microti M. pinipedii M. tuberculosis BCG 15
1 2 6 16 17 RFP Xpert MTB Rif RFP DNA RNA RNA 2 α 1 1 -subunit rpob - subunit rpob RFP RNA RFP RRDR Refampicin Resistance Determining Region, 81bp RFP 95 rpob RFP Rifabutin 18 rpob Rif TB PCR rpob RRDR DNA Line Probe Assay ; LPA 95 100 19, 20 INH fura-katg, fabg1-inha, 21 European-American katg 315 inha -15 MTBDRplus Hain Lifescience, Germany LPA 22 embc-emba-embb / EMB R gid / SM R gyrb-gyra / FQs R rpob / RIF R rpsl / SM R M. tuberculosis H37Rv 4.4 Mbp rrs / KM R, AMK R, KM R, CPM R fabg1-inha / INH R oxyr ahpc / INH R pnca / PZA R fura-katg / INH R
PZA PZA ph 10 MGIT AST PZA 42.1 23 PZA 24 PZA PZA pnca Pyrazinoic acid pnca PZA pnca 2012 12 PZA TB LPA 308 PZA 58 89.7 96.0 PCR LPA 100 25 Genotyping Restriction Fragment Length Polymorphisms : RFLP Gold standard Variable Number Tandem Repeat : VNTR VNTR PCR VNTR Whole Genome Sequence : WGS pnca 5 pnca 7 pnca 1-47 pnca 20 pnca 21 pnca 20 pnca 21 pnca : WT pnca : D12A pnca : 59 deletion c g232a pnca : 365-374 deletion PZA TB PZA
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